Journal: Oncotarget

Article Title: Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes

doi: 10.18632/oncotarget.14056

Figure Lengend Snippet: ( A ) Immunofluorescence images of H3K4me2 in oocytes after different treatments. Red, H3K4me2; Blue, DAPI staining of DNA. Scale bar = 20 μm. ( B ) Average H3K4me2 fluorescence intensity in oocytes. ( C ) ASH1L mRNA levels in oocytes from each group. ( D ) Immunofluorescence images of H3K9me2 in oocytes after different treatment. Red, H3K9me2; Blue, DAPI staining of DNA. Scale bar = 20 μm. ( E ) Average H3K9me2 fluorescence intensity in oocytes. ( F ) SETDB1 mRNA levels in oocytes from each group. ( G ) Immunofluorescence images of H3K27me3 in oocytes after different treatment. Red, H3K27me3; Blue, DAPI staining of DNA. Scale bar = 20 μm. ( H ) Average H3K27me3 fluorescence intensity in oocytes. ( I ) EZH2 mRNA levels in oocytes from each group.

Article Snippet: Briefy, oocytes were fixed with 4% paraformaldehyde at room temperature for 30 min and then transferred to a permeabilization solution (0.5% Triton X-100) for 30 min. After 1 h in 1% bull serum albumin (BSA)-supplemented PBS, oocytes were incubated at 4°C overnight with the following primary antibodies: 1:100 rabbit polyclonal anti-Di-methyl-Histone H3 (Lys9) (H3K9me2) (Abcam, UK), 1:100 Rabbit monoclonal anti-Di-methyl-Histone H3 (Lys27) (H3K27me2) (Cell Signaling Technology, USA), 1:100 rabbit polyclonal anti-Di-methyl-Histone H3 (Lys4) (H3K4me2) (Cell Signaling Technology, USA), 1:200 mouse anti-α-tubulin-FITC antibody (Sigma, USA) or 1:100 Phalloidin-TRITC (Sigma, USA).

Techniques: Immunofluorescence, Staining, Fluorescence