rabbit monoclonal anti pkc ε  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti pkc ε
    (A). Novel <t>PKC</t> isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, <t>β1,</t> <t>δ,</t> ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.
    Rabbit Monoclonal Anti Pkc ε, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti pkc ε/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti pkc ε - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Quantitative Kinetic Study of the Actin-Bundling Protein L-Plastin and of Its Impact on Actin Turn-Over"

    Article Title: Quantitative Kinetic Study of the Actin-Bundling Protein L-Plastin and of Its Impact on Actin Turn-Over

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0009210

    (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.
    Figure Legend Snippet: (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.

    Techniques Used: Western Blot, Translocation Assay, Immunofluorescence, Labeling, Microscopy, Staining, Transfection, Negative Control

    rabbit monoclonal anti pkc ε  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti pkc ε
    (A). Novel <t>PKC</t> isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, <t>β1,</t> <t>δ,</t> ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.
    Rabbit Monoclonal Anti Pkc ε, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti pkc ε/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti pkc ε - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Quantitative Kinetic Study of the Actin-Bundling Protein L-Plastin and of Its Impact on Actin Turn-Over"

    Article Title: Quantitative Kinetic Study of the Actin-Bundling Protein L-Plastin and of Its Impact on Actin Turn-Over

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0009210

    (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.
    Figure Legend Snippet: (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.

    Techniques Used: Western Blot, Translocation Assay, Immunofluorescence, Labeling, Microscopy, Staining, Transfection, Negative Control

    monoclonal rabbit p65 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal rabbit p65 antibody
    Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB <t>p65</t> and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.
    Monoclonal Rabbit P65 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    monoclonal rabbit p65 antibody - by Bioz Stars, 2023-01
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    1) Product Images from "Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation"

    Article Title: Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S157109

    Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB p65 and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.
    Figure Legend Snippet: Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB p65 and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.

    Techniques Used: Expressing, Western Blot

    The association between miR-146a, cytokines and NF-κB. Correlation between ( A ) miR-146a and IL-1β ( r 2 =0.7683, P <0.01), ( B ) TNF-α ( r 2 =0.8491, P <0.01), ( C ) NF-κB p50 ( r 2 =0.8898, P <0.01) and ( D ) NF-κB p65 ( r 2 =0.8945, P <0.01). Abbreviations: miR-146a, microRNA-146a; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; IL-1β, interleukin 1 beta; TNF-α, tumor necrosis factor alpha.
    Figure Legend Snippet: The association between miR-146a, cytokines and NF-κB. Correlation between ( A ) miR-146a and IL-1β ( r 2 =0.7683, P <0.01), ( B ) TNF-α ( r 2 =0.8491, P <0.01), ( C ) NF-κB p50 ( r 2 =0.8898, P <0.01) and ( D ) NF-κB p65 ( r 2 =0.8945, P <0.01). Abbreviations: miR-146a, microRNA-146a; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; IL-1β, interleukin 1 beta; TNF-α, tumor necrosis factor alpha.

    Techniques Used:

    rabbit anti ribosomal large protein l7  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ribosomal large protein l7
    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
    Rabbit Anti Ribosomal Large Protein L7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus"

    Article Title: Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0026120

    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, L7 ribosomal protein and GAPDH. Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
    Figure Legend Snippet: A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, L7 ribosomal protein and GAPDH. Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)

    Techniques Used: Expressing

    Aliquots of native polyribosomes and EDTA treated polyribosomes were loaded onto linear 15–45% (w/w) sucrose gradients and centrifuged for 2 hr at 34 000 rpm at 4°C in a Beckman SW40 rotor. Each collected fraction was assayed for the presence of FXR1P and L7 ribosomal protein. Fractions from the top to the bottom of the gradient are shown from left to right and the position of the 80 S ribosome monomer is indicated. SS, LS: ribosomal small and large subunits, respectively.
    Figure Legend Snippet: Aliquots of native polyribosomes and EDTA treated polyribosomes were loaded onto linear 15–45% (w/w) sucrose gradients and centrifuged for 2 hr at 34 000 rpm at 4°C in a Beckman SW40 rotor. Each collected fraction was assayed for the presence of FXR1P and L7 ribosomal protein. Fractions from the top to the bottom of the gradient are shown from left to right and the position of the 80 S ribosome monomer is indicated. SS, LS: ribosomal small and large subunits, respectively.

    Techniques Used:

    rabbit monoclonal antibody against protein disulfide isomerase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal antibody against protein disulfide isomerase
    Rabbit Monoclonal Antibody Against Protein Disulfide Isomerase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ab 2636978  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ab 2636978
    Ab 2636978, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti nucleocapsid  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti nucleocapsid
    Anti Nucleocapsid, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti nucleocapsid rabbit monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti nucleocapsid rabbit monoclonal antibody
    Anti Nucleocapsid Rabbit Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nucleocapsid rabbit monoclonal antibody/product/Cell Signaling Technology Inc
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    ab 2636921  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ab 2636921
    Ab 2636921, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ab 2636921/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit monoclonal anti p65  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti p65
    (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and <t>p65</t> proteins in PC-3 cells.
    Rabbit Monoclonal Anti P65, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p65/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit monoclonal anti p65 - by Bioz Stars, 2023-01
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    1) Product Images from "Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression"

    Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2018.10.034

    (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and p65 proteins in PC-3 cells.
    Figure Legend Snippet: (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and p65 proteins in PC-3 cells.

    Techniques Used: Western Blot, Immunoprecipitation

    (A) (Top) Schematic diagram depicting CDK4/CYCLIN D phosphorylation sites (P letter) of RB-N. (Bottom) Western blot analysis of p65 proteins in PC-3 whole-cell lysate (WCL) pulled down by GST-RB. Asterisks indicate proteins at the expected molecular weight.
    Figure Legend Snippet: (A) (Top) Schematic diagram depicting CDK4/CYCLIN D phosphorylation sites (P letter) of RB-N. (Bottom) Western blot analysis of p65 proteins in PC-3 whole-cell lysate (WCL) pulled down by GST-RB. Asterisks indicate proteins at the expected molecular weight.

    Techniques Used: Western Blot, Molecular Weight

    (A) CYCLIN D/CDK4/6-mediated RB S249/T252 phosphorylation promotes specific RB-p65 interaction, thereby triggering inhibition of expression of NF-κB target genes such as PD-L1 and cancer immunity.
    Figure Legend Snippet: (A) CYCLIN D/CDK4/6-mediated RB S249/T252 phosphorylation promotes specific RB-p65 interaction, thereby triggering inhibition of expression of NF-κB target genes such as PD-L1 and cancer immunity.

    Techniques Used: Inhibition, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software

    antibodies anti nucleocapsid  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies anti nucleocapsid
    Antibodies Anti Nucleocapsid, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit monoclonal anti pkc ε
    (A). Novel <t>PKC</t> isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, <t>β1,</t> <t>δ,</t> ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.
    Rabbit Monoclonal Anti Pkc ε, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB <t>p65</t> and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.
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    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    Cell Signaling Technology Inc ab 2636978
    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    Cell Signaling Technology Inc anti nucleocapsid
    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    Cell Signaling Technology Inc ab 2636921
    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, <t>L7</t> ribosomal protein <t>and</t> <t>GAPDH.</t> Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)
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    Cell Signaling Technology Inc rabbit monoclonal anti p65
    (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and <t>p65</t> proteins in PC-3 cells.
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    (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and <t>p65</t> proteins in PC-3 cells.
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    Image Search Results


    (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.

    Journal: PLoS ONE

    Article Title: Quantitative Kinetic Study of the Actin-Bundling Protein L-Plastin and of Its Impact on Actin Turn-Over

    doi: 10.1371/journal.pone.0009210

    Figure Lengend Snippet: (A). Novel PKC isozymes regulate PMA-induced L-plastin phosphorylation in MCF-7 cells. MCF-7 cells were pretreated for 3 hours with 5 µM of GF109203X (specific for α, β1, δ, ε, and ζ) or 0.5 µM of Gö6976 (specific for α and β1) and then treated for 1 hour with or without 1 µM PMA at 37°C. Total cell extracts (50 µg) were analyzed by immunoblotting using anti-Ser5-P L-plastin (upper panel) or anti-GAPDH (lower panel) antibodies. (B). PMA-induced actin cytoskeleton remodeling and L-plastin translocation involves novel PKC isozymes. MCF-7 cells were pretreated for 3 hours with 5 µM GF109203X or 0.5 µM Gö6976, then treated for 1 hour with 1 µM PMA at 37°C. Cells were then fixed and processed for immunofluorescence. Labeled cells were analyzed with an epifluorescence microscope after staining with an anti-L-plastin antibody and Alexa 488-conjugated phalloidin. Scale bar, 10 µm. (C). SiRNA knock-down of PKC-δ decreased PMA-induced L-plastin phosphorylation in MCF-7. MCF-7 cells were transfected with either PKC-δ or PKC-ε siRNAs as well as with negative control siRNA (Ctrl) for 48 hours and then treated with PMA as indicated. Total cell extracts (50 µg) were analyzed by immunoblotting using antibodies specific for Ser5 phosphorylated L-plastin, PKC-ε, PKC-δ and total L-plastin. GAPDH was used to monitor equal protein loading.

    Article Snippet: Rabbit polyclonal anti-PKC-δ and rabbit monoclonal anti-PKC-ε were from Cell Signaling Technology, Inc. (Danvers, MA).

    Techniques: Western Blot, Translocation Assay, Immunofluorescence, Labeling, Microscopy, Staining, Transfection, Negative Control

    Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB p65 and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.

    Journal: Drug Design, Development and Therapy

    Article Title: Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation

    doi: 10.2147/DDDT.S157109

    Figure Lengend Snippet: Expression of NF-κB in the sciatic nerve of each group. ( A ) Western blot analysis showed the expression of NF-κB p65 and p50 in the sciatic nerve of each group. ( B ) The relative expression of NF-κB p65 and p50 normalized to β-actin ( # P <0.01). Abbreviations: NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.

    Article Snippet: The primary antibodies used were a monoclonal rabbit p65 antibody (1:1,000 dilution; Cell Signaling Technology, Danvers, MA, USA), p50 antibody (1:1,000 dilution; Cell Signaling Technology) and an anti-β-actin primary antibody (1:5,000 dilution; Proteintech Group Inc., Chicago, IL, USA).

    Techniques: Expressing, Western Blot

    The association between miR-146a, cytokines and NF-κB. Correlation between ( A ) miR-146a and IL-1β ( r 2 =0.7683, P <0.01), ( B ) TNF-α ( r 2 =0.8491, P <0.01), ( C ) NF-κB p50 ( r 2 =0.8898, P <0.01) and ( D ) NF-κB p65 ( r 2 =0.8945, P <0.01). Abbreviations: miR-146a, microRNA-146a; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; IL-1β, interleukin 1 beta; TNF-α, tumor necrosis factor alpha.

    Journal: Drug Design, Development and Therapy

    Article Title: Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation

    doi: 10.2147/DDDT.S157109

    Figure Lengend Snippet: The association between miR-146a, cytokines and NF-κB. Correlation between ( A ) miR-146a and IL-1β ( r 2 =0.7683, P <0.01), ( B ) TNF-α ( r 2 =0.8491, P <0.01), ( C ) NF-κB p50 ( r 2 =0.8898, P <0.01) and ( D ) NF-κB p65 ( r 2 =0.8945, P <0.01). Abbreviations: miR-146a, microRNA-146a; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; IL-1β, interleukin 1 beta; TNF-α, tumor necrosis factor alpha.

    Article Snippet: The primary antibodies used were a monoclonal rabbit p65 antibody (1:1,000 dilution; Cell Signaling Technology, Danvers, MA, USA), p50 antibody (1:1,000 dilution; Cell Signaling Technology) and an anti-β-actin primary antibody (1:5,000 dilution; Proteintech Group Inc., Chicago, IL, USA).

    Techniques:

    A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, L7 ribosomal protein and GAPDH. Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)

    Journal: PLoS ONE

    Article Title: Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus

    doi: 10.1371/journal.pone.0026120

    Figure Lengend Snippet: A. Hippocampal lysates were prepared from mice at different developmental stages (P2 = postnatal day 2) and analyzed for FXR1P, FXR2P, FMRP, L7 ribosomal protein and GAPDH. Isoforms of FXR1P (a.b,c,d), FXR2P, FMRP and ribosomal protein L7 were all highly expressed during early postnatal development in the hippocampus. B. FXR1P (isoforms c, d) expression across postnatal development was quantified and normalized against GAPDH expression. FXR1P levels decreased relative to GAPDH. C. We immunostained cryostat sections prepared from a P14 mouse with #ML13 and imaged the hippocampus at 10X (left panel). We found that FXR1P was highly expressed in neurons at P14. A 60X image of pyramidal neurons in area CA1 of the hippocampus (box in 10X image) showing FXR1P expression in the cell body and proximal dendrites of CA1 neurons (right panel). Arrow points to a proximal dendrite found in the plane of the image. Scale bar = 60 µm (low magnification) and 10 µm (high magnification)

    Article Snippet: Other antibodies used included mouse monoclonal antibodies against FMRP (mAb1C3; ), FXR1P (mAb3FX; ), FXR2P (mAbA42, Abcam), myc (Santa Cruz; 9E10), MAP-2 (Sigma-Aldrich; HM-2)and GAPDH (Abcam; ab9484), human anti-ribosomal P antibodies (Immunovision), a rabbit anti-ribosomal large protein L7 (Cell Signaling), a rabbit monoclonal antibody against S6 (Cell Signaling; 5G10) and a goat polyclonal antibody against TIA-1 (Santa Cruz; sc-1751).

    Techniques: Expressing

    Aliquots of native polyribosomes and EDTA treated polyribosomes were loaded onto linear 15–45% (w/w) sucrose gradients and centrifuged for 2 hr at 34 000 rpm at 4°C in a Beckman SW40 rotor. Each collected fraction was assayed for the presence of FXR1P and L7 ribosomal protein. Fractions from the top to the bottom of the gradient are shown from left to right and the position of the 80 S ribosome monomer is indicated. SS, LS: ribosomal small and large subunits, respectively.

    Journal: PLoS ONE

    Article Title: Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus

    doi: 10.1371/journal.pone.0026120

    Figure Lengend Snippet: Aliquots of native polyribosomes and EDTA treated polyribosomes were loaded onto linear 15–45% (w/w) sucrose gradients and centrifuged for 2 hr at 34 000 rpm at 4°C in a Beckman SW40 rotor. Each collected fraction was assayed for the presence of FXR1P and L7 ribosomal protein. Fractions from the top to the bottom of the gradient are shown from left to right and the position of the 80 S ribosome monomer is indicated. SS, LS: ribosomal small and large subunits, respectively.

    Article Snippet: Other antibodies used included mouse monoclonal antibodies against FMRP (mAb1C3; ), FXR1P (mAb3FX; ), FXR2P (mAbA42, Abcam), myc (Santa Cruz; 9E10), MAP-2 (Sigma-Aldrich; HM-2)and GAPDH (Abcam; ab9484), human anti-ribosomal P antibodies (Immunovision), a rabbit anti-ribosomal large protein L7 (Cell Signaling), a rabbit monoclonal antibody against S6 (Cell Signaling; 5G10) and a goat polyclonal antibody against TIA-1 (Santa Cruz; sc-1751).

    Techniques:

    (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and p65 proteins in PC-3 cells.

    Journal: Molecular cell

    Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

    doi: 10.1016/j.molcel.2018.10.034

    Figure Lengend Snippet: (A and B) Western blot analysis of reciprocally co-immunoprecipitated endogenous RB and p65 proteins in PC-3 cells.

    Article Snippet: Rabbit monoclonal anti-p65 , Cell Signaling Technology , Cat# 8242; RRID: AB_10859369.

    Techniques: Western Blot, Immunoprecipitation

    (A) (Top) Schematic diagram depicting CDK4/CYCLIN D phosphorylation sites (P letter) of RB-N. (Bottom) Western blot analysis of p65 proteins in PC-3 whole-cell lysate (WCL) pulled down by GST-RB. Asterisks indicate proteins at the expected molecular weight.

    Journal: Molecular cell

    Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

    doi: 10.1016/j.molcel.2018.10.034

    Figure Lengend Snippet: (A) (Top) Schematic diagram depicting CDK4/CYCLIN D phosphorylation sites (P letter) of RB-N. (Bottom) Western blot analysis of p65 proteins in PC-3 whole-cell lysate (WCL) pulled down by GST-RB. Asterisks indicate proteins at the expected molecular weight.

    Article Snippet: Rabbit monoclonal anti-p65 , Cell Signaling Technology , Cat# 8242; RRID: AB_10859369.

    Techniques: Western Blot, Molecular Weight

    (A) CYCLIN D/CDK4/6-mediated RB S249/T252 phosphorylation promotes specific RB-p65 interaction, thereby triggering inhibition of expression of NF-κB target genes such as PD-L1 and cancer immunity.

    Journal: Molecular cell

    Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

    doi: 10.1016/j.molcel.2018.10.034

    Figure Lengend Snippet: (A) CYCLIN D/CDK4/6-mediated RB S249/T252 phosphorylation promotes specific RB-p65 interaction, thereby triggering inhibition of expression of NF-κB target genes such as PD-L1 and cancer immunity.

    Article Snippet: Rabbit monoclonal anti-p65 , Cell Signaling Technology , Cat# 8242; RRID: AB_10859369.

    Techniques: Inhibition, Expressing

    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

    doi: 10.1016/j.molcel.2018.10.034

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit monoclonal anti-p65 , Cell Signaling Technology , Cat# 8242; RRID: AB_10859369.

    Techniques: Recombinant, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software