calpain 1  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc calpain 1
    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, <t>BiP,</t> <t>calpain-1</t> and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.
    Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis"

    Article Title: Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050407

    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.
    Figure Legend Snippet: (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Western Blot, Expressing, Immunohistochemistry

    calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc calpain 1
    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, <t>BiP,</t> <t>calpain-1</t> and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.
    Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis"

    Article Title: Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050407

    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.
    Figure Legend Snippet: (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Western Blot, Expressing, Immunohistochemistry

    anti calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc anti calpain 1
    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and <t>CAPN1)</t> genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression"

    Article Title: Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050786

    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Figure Legend Snippet: Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.

    Techniques Used: Western Blot

    anti calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc anti calpain 1
    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and <t>CAPN1)</t> genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression"

    Article Title: Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0050786

    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Figure Legend Snippet: Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.

    Techniques Used: Western Blot

    anti calpain-1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc anti calpain-1
    Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calpain-1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti calpain-1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc calpain 1
    Aloe-emodin modulated ER stress involving in cytosolic calcium overload and calpain/caspase-12 pathway in SW620 and HT29 cells. ( A ) SW620 cells and ( B ) HT29 cells were treated with indicated concentrations of Aloe-emodin for 24 hours, then stained with Fluo4-AM (2 μM); the cytosolic calcium levels were quantitatively analyzed through flow cytometry; ( C–F ) <t>The</t> <t>calpain-1,</t> calpain-2, and caspase-12 proteins expression in SW620 cells were analyzed by western blot; ( G–J ) The calpain-1, calpain-2, and caspase-12 proteins expression in HT29 cells were analyzed by western blot. * P <0.05, ** P <0.01, *** P <0.001, as compared with the control group; n=4.
    Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Aloe-Emodin Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Colorectal Cancer Cells"

    Article Title: Aloe-Emodin Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Colorectal Cancer Cells

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.908400

    Aloe-emodin modulated ER stress involving in cytosolic calcium overload and calpain/caspase-12 pathway in SW620 and HT29 cells. ( A ) SW620 cells and ( B ) HT29 cells were treated with indicated concentrations of Aloe-emodin for 24 hours, then stained with Fluo4-AM (2 μM); the cytosolic calcium levels were quantitatively analyzed through flow cytometry; ( C–F ) The calpain-1, calpain-2, and caspase-12 proteins expression in SW620 cells were analyzed by western blot; ( G–J ) The calpain-1, calpain-2, and caspase-12 proteins expression in HT29 cells were analyzed by western blot. * P <0.05, ** P <0.01, *** P <0.001, as compared with the control group; n=4.
    Figure Legend Snippet: Aloe-emodin modulated ER stress involving in cytosolic calcium overload and calpain/caspase-12 pathway in SW620 and HT29 cells. ( A ) SW620 cells and ( B ) HT29 cells were treated with indicated concentrations of Aloe-emodin for 24 hours, then stained with Fluo4-AM (2 μM); the cytosolic calcium levels were quantitatively analyzed through flow cytometry; ( C–F ) The calpain-1, calpain-2, and caspase-12 proteins expression in SW620 cells were analyzed by western blot; ( G–J ) The calpain-1, calpain-2, and caspase-12 proteins expression in HT29 cells were analyzed by western blot. * P <0.05, ** P <0.01, *** P <0.001, as compared with the control group; n=4.

    Techniques Used: Staining, Flow Cytometry, Expressing, Western Blot

    rabbit anti calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc rabbit anti calpain 1
    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and <t>for</t> <t>calpain-1</t> (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.
    Rabbit Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Calpain-mediated proteolysis of vimentin filaments is augmented in giant axonal neuropathy fibroblasts exposed to hypotonic stress"

    Article Title: Calpain-mediated proteolysis of vimentin filaments is augmented in giant axonal neuropathy fibroblasts exposed to hypotonic stress

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2022.1008542

    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and for calpain-1 (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.
    Figure Legend Snippet: Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and for calpain-1 (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.

    Techniques Used: Western Blot, Inhibition

    calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc calpain 1
    Antioxidants supplementation effects upon qualitative parameters of stored red blood cells. Hemolysis parameters (A), pH (B), calcium accumulation along <t>with</t> <t>calpain-1</t> recruitment to the membrane (C) and extracellular antioxidant capacity (D) during storage of red blood cells under standard conditions or upon supplementation with uric acid (UA) and/or ascorbic acid (AA). Representative immunoblots are shown (n = 6). 4.1R protein was used as internal loading control. Data are presented as mean ± SD. C: control samples. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Supplementation with uric and ascorbic acid protects stored red blood cells through enhancement of non-enzymatic antioxidant activity and metabolic rewiring"

    Article Title: Supplementation with uric and ascorbic acid protects stored red blood cells through enhancement of non-enzymatic antioxidant activity and metabolic rewiring

    Journal: Redox Biology

    doi: 10.1016/j.redox.2022.102477

    Antioxidants supplementation effects upon qualitative parameters of stored red blood cells. Hemolysis parameters (A), pH (B), calcium accumulation along with calpain-1 recruitment to the membrane (C) and extracellular antioxidant capacity (D) during storage of red blood cells under standard conditions or upon supplementation with uric acid (UA) and/or ascorbic acid (AA). Representative immunoblots are shown (n = 6). 4.1R protein was used as internal loading control. Data are presented as mean ± SD. C: control samples. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Antioxidants supplementation effects upon qualitative parameters of stored red blood cells. Hemolysis parameters (A), pH (B), calcium accumulation along with calpain-1 recruitment to the membrane (C) and extracellular antioxidant capacity (D) during storage of red blood cells under standard conditions or upon supplementation with uric acid (UA) and/or ascorbic acid (AA). Representative immunoblots are shown (n = 6). 4.1R protein was used as internal loading control. Data are presented as mean ± SD. C: control samples. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Western Blot

    rabbit anti calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc rabbit anti calpain 1
    Increased mitochondrial NOX4 expression in Nox4 TG618 mice is associated with upregulated L-type Ca 2+ current ( I CaL ), increased expression of calpain 2 and fractured sarcomeric Z-discs in cardiomyocytes. (A) Representative recordings of the I CaL in wild-type and Nox4 TG618 mice elicited at 10 mV, (B) voltage-current curves (Vm), (C) conductance (G), (D) normalized conductance (G/Gmax), and (E) membrane capacitance. For I CaL recordings, cardiomyocytes (n = 27 wild-type and 30 Nox4 TG618) from 4 mice of each genotype were used. (F) A normalized weight for the heart based on the length of the tibia; (N = 4). (G) Representative Western blot analysis and quantification of expression of calpain1/2 <t>(CAPN1/2)</t> and calpastatin (CAST) (N = 6) in LV lysates. (H) Representative TEM images of murine LV cross sections with focus on Z-disc (N = 3). The data are presented as mean ± SEM.
    Rabbit Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Mitochondrial oxidative stress contributes to diastolic dysfunction through impaired mitochondrial dynamics"

    Article Title: Mitochondrial oxidative stress contributes to diastolic dysfunction through impaired mitochondrial dynamics

    Journal: Redox Biology

    doi: 10.1016/j.redox.2022.102474

    Increased mitochondrial NOX4 expression in Nox4 TG618 mice is associated with upregulated L-type Ca 2+ current ( I CaL ), increased expression of calpain 2 and fractured sarcomeric Z-discs in cardiomyocytes. (A) Representative recordings of the I CaL in wild-type and Nox4 TG618 mice elicited at 10 mV, (B) voltage-current curves (Vm), (C) conductance (G), (D) normalized conductance (G/Gmax), and (E) membrane capacitance. For I CaL recordings, cardiomyocytes (n = 27 wild-type and 30 Nox4 TG618) from 4 mice of each genotype were used. (F) A normalized weight for the heart based on the length of the tibia; (N = 4). (G) Representative Western blot analysis and quantification of expression of calpain1/2 (CAPN1/2) and calpastatin (CAST) (N = 6) in LV lysates. (H) Representative TEM images of murine LV cross sections with focus on Z-disc (N = 3). The data are presented as mean ± SEM.
    Figure Legend Snippet: Increased mitochondrial NOX4 expression in Nox4 TG618 mice is associated with upregulated L-type Ca 2+ current ( I CaL ), increased expression of calpain 2 and fractured sarcomeric Z-discs in cardiomyocytes. (A) Representative recordings of the I CaL in wild-type and Nox4 TG618 mice elicited at 10 mV, (B) voltage-current curves (Vm), (C) conductance (G), (D) normalized conductance (G/Gmax), and (E) membrane capacitance. For I CaL recordings, cardiomyocytes (n = 27 wild-type and 30 Nox4 TG618) from 4 mice of each genotype were used. (F) A normalized weight for the heart based on the length of the tibia; (N = 4). (G) Representative Western blot analysis and quantification of expression of calpain1/2 (CAPN1/2) and calpastatin (CAST) (N = 6) in LV lysates. (H) Representative TEM images of murine LV cross sections with focus on Z-disc (N = 3). The data are presented as mean ± SEM.

    Techniques Used: Expressing, Western Blot

    LV samples of diastolic dysfunction patients show increased fibrosis and myofibroblast activation/ Representative immunofluorescent images and quantification of (A) picrosirius red staining, (B) collagen I, (C) periostin, and (D) ACTA2 in human LV cross sections and counterstained for cardiomyocyte marker MYH. Western blot analysis and quantification of expression of proteins involved in mitochondrial fission (E) , fusion (F), and of calpain 1/2 (CAPN1/2) and calpastatin (CAST) (G) in human LV myocardial lysates. The data are presented as mean ± SEM, N = 3. Scale is 100 μm. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: LV samples of diastolic dysfunction patients show increased fibrosis and myofibroblast activation/ Representative immunofluorescent images and quantification of (A) picrosirius red staining, (B) collagen I, (C) periostin, and (D) ACTA2 in human LV cross sections and counterstained for cardiomyocyte marker MYH. Western blot analysis and quantification of expression of proteins involved in mitochondrial fission (E) , fusion (F), and of calpain 1/2 (CAPN1/2) and calpastatin (CAST) (G) in human LV myocardial lysates. The data are presented as mean ± SEM, N = 3. Scale is 100 μm. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Activation Assay, Staining, Marker, Western Blot, Expressing

    rabbit anti calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc rabbit anti calpain 1
    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton-X insoluble pellet fractions from untreated or water-exposed GAN fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin <t>(top),</t> <t>calpain-1</t> (middle top), calpain-2 (middle), calpastatin (middle bottom), and actin (bottom; loading control) in reduced Triton-X insoluble and soluble fractions from GAN and control fibroblasts exposed to 8 minutes of hypotonic stress and treated with the DMSO vehicle control or MG-132. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full-length vimentin band for each cell line and treatment group (from panel B). ****p<0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels from panel B. **p<0.01; ****p<0.0001; two-way ANOVA.
    Rabbit Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Calpain-mediated proteolysis of vimentin filaments is augmented in Giant Axonal Neuropathy (GAN) fibroblasts exposed to hypotonic stress"

    Article Title: Calpain-mediated proteolysis of vimentin filaments is augmented in Giant Axonal Neuropathy (GAN) fibroblasts exposed to hypotonic stress

    Journal: bioRxiv

    doi: 10.1101/2022.07.31.501244

    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton-X insoluble pellet fractions from untreated or water-exposed GAN fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top), calpain-1 (middle top), calpain-2 (middle), calpastatin (middle bottom), and actin (bottom; loading control) in reduced Triton-X insoluble and soluble fractions from GAN and control fibroblasts exposed to 8 minutes of hypotonic stress and treated with the DMSO vehicle control or MG-132. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full-length vimentin band for each cell line and treatment group (from panel B). ****p<0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels from panel B. **p<0.01; ****p<0.0001; two-way ANOVA.
    Figure Legend Snippet: Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton-X insoluble pellet fractions from untreated or water-exposed GAN fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top), calpain-1 (middle top), calpain-2 (middle), calpastatin (middle bottom), and actin (bottom; loading control) in reduced Triton-X insoluble and soluble fractions from GAN and control fibroblasts exposed to 8 minutes of hypotonic stress and treated with the DMSO vehicle control or MG-132. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full-length vimentin band for each cell line and treatment group (from panel B). ****p<0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels from panel B. **p<0.01; ****p<0.0001; two-way ANOVA.

    Techniques Used: Western Blot, Inhibition

    antibodies against calpain 1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc antibodies against calpain 1
    <t>Calpain</t> <t>1</t> and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of <t>Calpain</t> <t>1</t> (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.
    Antibodies Against Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against calpain 1 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein"

    Article Title: Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2022.811980

    Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of Calpain 1 (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.
    Figure Legend Snippet: Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of Calpain 1 (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.

    Techniques Used: Immunohistochemical staining, Expressing

    Calpeptin inhibits AIM2 and NLRP3 inflammasome-mediated inflammation, and upregulates Klotho protein expression in IR mouse model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, IL-1β and IL-18 in the kidney of all mice. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1 and IL-18. (C) The mRNA levels of Calpain 2, Klotho, AIM2, ASC and GSDMD in the kidney among different groups. (D) The calpain activity of renal issues was measured by the relative fluorescence units (400/505 nm). (E) The cathepsin B activity of kidney issues was tested by the relative fluorescence units (400/505 nm). (F) Representative immunohistochemical micrographs from the kidney issues of different groups stained with Calpain 1, Calpain 2 and AIM2. ×400, bar = 50 μm. All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. sham group; ** p < 0.01 vs. sham group; *** p < 0.001 vs. sham group; # p < 0.05 vs. IR group; ## p < 0.01 vs. IR group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion; RFU, relative fluorescence units.
    Figure Legend Snippet: Calpeptin inhibits AIM2 and NLRP3 inflammasome-mediated inflammation, and upregulates Klotho protein expression in IR mouse model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, IL-1β and IL-18 in the kidney of all mice. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1 and IL-18. (C) The mRNA levels of Calpain 2, Klotho, AIM2, ASC and GSDMD in the kidney among different groups. (D) The calpain activity of renal issues was measured by the relative fluorescence units (400/505 nm). (E) The cathepsin B activity of kidney issues was tested by the relative fluorescence units (400/505 nm). (F) Representative immunohistochemical micrographs from the kidney issues of different groups stained with Calpain 1, Calpain 2 and AIM2. ×400, bar = 50 μm. All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. sham group; ** p < 0.01 vs. sham group; *** p < 0.001 vs. sham group; # p < 0.05 vs. IR group; ## p < 0.01 vs. IR group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion; RFU, relative fluorescence units.

    Techniques Used: Expressing, Western Blot, Activity Assay, Fluorescence, Immunohistochemical staining, Staining

    Calpeptin inhibits Calpain 1 activation and GSDMD cleavage in the tubules of IR mice, reduces cells apoptosis as well. (A,B) Representative immunofluorescence micrographs from mice kidneys of different groups stained Calpain 1 and GSDMD. ×400, bar = 50 μm. (C) Representative TUNEL staining micrographs in the mice kidneys of different groups. ×400, bar = 50 μm. (D) Quantitative determination of apoptosis cells amount. All Values were presented as mean ± SEM ( n = 3). *** p < 0.001 vs. sham group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion.
    Figure Legend Snippet: Calpeptin inhibits Calpain 1 activation and GSDMD cleavage in the tubules of IR mice, reduces cells apoptosis as well. (A,B) Representative immunofluorescence micrographs from mice kidneys of different groups stained Calpain 1 and GSDMD. ×400, bar = 50 μm. (C) Representative TUNEL staining micrographs in the mice kidneys of different groups. ×400, bar = 50 μm. (D) Quantitative determination of apoptosis cells amount. All Values were presented as mean ± SEM ( n = 3). *** p < 0.001 vs. sham group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, TUNEL Assay

    Calpeptin suppresses AIM2 and NLRP3 inflammasome signaling pathway, and increases Klotho protein expression in CoCl 2 -induced HK-2 cells hypoxia model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, ASC, IL-1β and LCN2 in the HK-2 cells. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1, IL-1β and LCN2. (C) The calpain activity of HK-2 cells was assessed by the relative fluorescence units (400/505 nm). (D) The cathepsin B activity of HK-2 cells was analyzed by the relative fluorescence units (400/505 nm). All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. control group; ** p < 0.01 vs. control group; *** p < 0.001 vs. control group; # p < 0.05 vs. CoCl 2 group; ## p < 0.01 vs. CoCl 2 group; ### p < 0.001 vs. CoCl 2 group. CP, calpeptin; RFU, relative fluorescence units.
    Figure Legend Snippet: Calpeptin suppresses AIM2 and NLRP3 inflammasome signaling pathway, and increases Klotho protein expression in CoCl 2 -induced HK-2 cells hypoxia model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, ASC, IL-1β and LCN2 in the HK-2 cells. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1, IL-1β and LCN2. (C) The calpain activity of HK-2 cells was assessed by the relative fluorescence units (400/505 nm). (D) The cathepsin B activity of HK-2 cells was analyzed by the relative fluorescence units (400/505 nm). All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. control group; ** p < 0.01 vs. control group; *** p < 0.001 vs. control group; # p < 0.05 vs. CoCl 2 group; ## p < 0.01 vs. CoCl 2 group; ### p < 0.001 vs. CoCl 2 group. CP, calpeptin; RFU, relative fluorescence units.

    Techniques Used: Expressing, Western Blot, Activity Assay, Fluorescence

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Cell Signaling Technology Inc calpain 1
    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, <t>BiP,</t> <t>calpain-1</t> and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.
    Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calpain 1 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti calpain 1
    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and <t>CAPN1)</t> genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti calpain-1
    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and <t>CAPN1)</t> genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.
    Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti calpain-1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti calpain-1 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc rabbit anti calpain 1
    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and <t>for</t> <t>calpain-1</t> (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.
    Rabbit Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti calpain 1 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc antibodies against calpain 1
    <t>Calpain</t> <t>1</t> and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of <t>Calpain</t> <t>1</t> (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.
    Antibodies Against Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against calpain 1/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against calpain 1 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    Image Search Results


    (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.

    Journal: PLoS ONE

    Article Title: Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis

    doi: 10.1371/journal.pone.0050407

    Figure Lengend Snippet: (A–E). Protein from colonic extracts was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by immunoblotting for CHOP, BiP, calpain-1 and caspase-12. CHOP, BiP, calpain-1 and caspase-12 were markedly expressed in rats treated with TNBS alone. However, glutamine administration partially abolished CHOP, BiP, calpain-1 and caspase-12 expression induced by TNBS. Results are representative of four independent experiments. Equal loading of proteins is illustrated by β-actin bands. (A) Representative Western-blot photographs for CHOP, calpain-1, BiP, caspase-12, and β-actin. (B) Densitometric quantification of CHOP. (C) Densitometric quantification of calpain-1. (D) Densitometric quantification of BiP. (E) Densitometric quantification of caspase-12. Data are expressed as mean ± S.E.M. from 8 rats. *P<0.05 compared with control group. # P<0.05 compared with TNBS group. & P<0.05 compared with same group 2 d. (F) Photomicrographs of immunohistochemistry for BiP in sections of colonic samples. Paraffin-embedded sections were immunostained with a BiP antibody. Original magnification: 200X.

    Article Snippet: The membranes were then blocked with 5% non-fat dry milk in phosphate buffered saline buffer containing 0.05% Tween 20 (PBST) for 1 hour at room temperature and probed overnight at 4°C with polyclonal anti-Bax, Bcl-2, Bcl-xL, poly(ADP-ribose) polymerase-1 (PARP-1), cytochrome c, transcription factor CHOP/GADD153 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), JNK, phospho-JNK, BiP/glucose-regulated protein 78 (GRP78), p53, phospho-p53, cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA), calpain-1, caspase-12 and phospho-IRE1 (Abcam, Cambridge, UK) antibodies at 1∶200–1∶1,000 dilution with PBST containing 3% non-fat dry milk.

    Techniques: Polyacrylamide Gel Electrophoresis, Western Blot, Expressing, Immunohistochemistry

    Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.

    Journal: PLoS ONE

    Article Title: Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression

    doi: 10.1371/journal.pone.0050786

    Figure Lengend Snippet: Immunoblot of anti-apoptotic (pAKT and PI3KR2) and pro-apoptotic (BAD, CASP6 and CAPN1) genes in GEM-R cell line and GEM-S cell lines. Cells were treated with gemcitabine and combination with gossypol for 48 hours. The results shown are representative of two independent experiments.

    Article Snippet: The respective primary antibodies were used as follows: anti-Bcl-xl (Cell Signaling; MA, US) at 1∶1000 dilution, anti-Bad (Cell Signaling; MA, US) at 1;1000 dilution, anti-Bak (Cell Signaling; MA, US) 1∶1000 dilution, anti-Bax (Cell Signaling; MA, US) 1∶3000 dilution, anti-caspase-6 (Cell Signaling; MA, US) 1∶1000 dilution, anti-P1K3R2 (Cell Signaling; MA, US) 1∶1000 dilution, anti-pAKT (Cell Signaling; MA, US) at 1∶1000 dilution, anti-calpain-1 (Cell Signaling; MA, US) at 1∶1000 dilution, anti-Actin (loading control; Cell Signaling; MA, US) at 1∶3000 dilution, anti-Bcl-2 (Santa Cruz Biotechnology; CA, USA) 1∶3000 dilution, and anti-Mcl-1 (Santa Cruz Biotechnology; CA, USA) at 1∶3000 dilution, and anti-Noxa (Calbiochem, Merck; Germany) 1∶1000 dilution.

    Techniques: Western Blot

    Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and for calpain-1 (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Calpain-mediated proteolysis of vimentin filaments is augmented in giant axonal neuropathy fibroblasts exposed to hypotonic stress

    doi: 10.3389/fcell.2022.1008542

    Figure Lengend Snippet: Vimentin cleavage is blocked by inhibitors of the proteasome and calpains, which are elevated in GAN. (A) Immunoblotting for vimentin (top) and actin (bottom; loading control) in Triton X insoluble pellet fractions from untreated (lanes 1–3, 7–9) or water-exposed (lanes 4–6, 10–12) control 56.3 and GAN 56.1 fibroblasts in the absence or presence of the DMSO vehicle control, calpain inhibitor (MDL-28170), or calpain/proteasome inhibitor (MG-132). Note partial cleavage inhibition by MDL and near-complete inhibition by MG-132. (B) Immunoblotting for vimentin (top) and actin (bottom; loading control) in reduced Triton X insoluble fractions and for calpain-1 (middle top), calpain-2 (middle), and calpastatin (middle bottom) in reduced Triton X soluble fractions from GAN and control fibroblasts exposed to 8 min of hypotonic stress and treated with the DMSO vehicle control or MG-132. Shown are technical replicates from an individual experiment. The experiment was independently repeated at least 3 times. (C) Quantification of the relative fragment 1 (FR1) and fragment 2 (FR2) band intensities normalized to the full length vimentin band for each cell line (GAN 56.1 fibroblasts; control 56.3 fibroblasts) and treatment group (from panel B). **** p < 0.0001; two-way ANOVA. (D) Quantification of calpain-1, calpain-2, and calpastatin levels (from panel B). ** p < 0.01; **** p < 0.0001; two-way ANOVA.

    Article Snippet: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1,000, IF 1:200), mouse anti-Vimentin (Invitrogen, V9, WB 1:1,000–1:2,500, IF 1:100), mouse anti-Actin (Thermo Fisher Scientific, ACTN05, WB 1:1,000), mouse anti-Actin (Santa Cruz, SPM161, WB 1:1,000), rabbit anti-Calpastatin (Cell Signaling Technology, WB 1:1,000), rabbit anti-Calpain 1 (Cell Signaling Technology, large subunit Mu-type, WB 1:1,000), rabbit anti-Calpain 2 (Cell Signaling Technology, large subunit M-type, WB 1:1,000), and rabbit anti-Calpain 2 (Cell Signaling Technology, large subunit M-type, E3M6E, IF 1:200).

    Techniques: Western Blot, Inhibition

    Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of Calpain 1 (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.

    Journal: Frontiers in Medicine

    Article Title: Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

    doi: 10.3389/fmed.2022.811980

    Figure Lengend Snippet: Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of Calpain 1 (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients ( n = 10) and normal control group ( n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM ( n = 3). ** p < 0.01; *** p < 0.001 vs. normal control group. AKI, acute kidney injury.

    Article Snippet: The membrane was blocked by protein free rapid blocking buffer (PS108P, Epizyme) for 20 min and then incubated overnight with primary antibodies against Calpain 1 (1:1000, #2556, Cell Signaling Technology), Calpain 2 (1:1000, # DF7807, Affinity), Klotho (1:500, # DF10309, Affinity), AIM2(1:500, # DF3514, Affinity), NLRP3 (1:500, # DF7438, Affinity), pro-Caspase 1 (1:500, #24232, Cell Signaling Technology), cleaved-Caspase 1 (1:500, #89332, Cell Signaling Technology), ASC (1:1000, sc-514414, Santa Cruz), IL-1β (1:500, #83186, Cell Signaling Technology), IL-18 (1:500, # DF6252, Affinity), LCN2 (1:500, #DF6816, Affinity), and GAPDH (1:10000, ab181602, Abcam).

    Techniques: Immunohistochemical staining, Expressing

    Calpeptin inhibits AIM2 and NLRP3 inflammasome-mediated inflammation, and upregulates Klotho protein expression in IR mouse model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, IL-1β and IL-18 in the kidney of all mice. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1 and IL-18. (C) The mRNA levels of Calpain 2, Klotho, AIM2, ASC and GSDMD in the kidney among different groups. (D) The calpain activity of renal issues was measured by the relative fluorescence units (400/505 nm). (E) The cathepsin B activity of kidney issues was tested by the relative fluorescence units (400/505 nm). (F) Representative immunohistochemical micrographs from the kidney issues of different groups stained with Calpain 1, Calpain 2 and AIM2. ×400, bar = 50 μm. All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. sham group; ** p < 0.01 vs. sham group; *** p < 0.001 vs. sham group; # p < 0.05 vs. IR group; ## p < 0.01 vs. IR group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion; RFU, relative fluorescence units.

    Journal: Frontiers in Medicine

    Article Title: Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

    doi: 10.3389/fmed.2022.811980

    Figure Lengend Snippet: Calpeptin inhibits AIM2 and NLRP3 inflammasome-mediated inflammation, and upregulates Klotho protein expression in IR mouse model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, IL-1β and IL-18 in the kidney of all mice. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1 and IL-18. (C) The mRNA levels of Calpain 2, Klotho, AIM2, ASC and GSDMD in the kidney among different groups. (D) The calpain activity of renal issues was measured by the relative fluorescence units (400/505 nm). (E) The cathepsin B activity of kidney issues was tested by the relative fluorescence units (400/505 nm). (F) Representative immunohistochemical micrographs from the kidney issues of different groups stained with Calpain 1, Calpain 2 and AIM2. ×400, bar = 50 μm. All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. sham group; ** p < 0.01 vs. sham group; *** p < 0.001 vs. sham group; # p < 0.05 vs. IR group; ## p < 0.01 vs. IR group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion; RFU, relative fluorescence units.

    Article Snippet: The membrane was blocked by protein free rapid blocking buffer (PS108P, Epizyme) for 20 min and then incubated overnight with primary antibodies against Calpain 1 (1:1000, #2556, Cell Signaling Technology), Calpain 2 (1:1000, # DF7807, Affinity), Klotho (1:500, # DF10309, Affinity), AIM2(1:500, # DF3514, Affinity), NLRP3 (1:500, # DF7438, Affinity), pro-Caspase 1 (1:500, #24232, Cell Signaling Technology), cleaved-Caspase 1 (1:500, #89332, Cell Signaling Technology), ASC (1:1000, sc-514414, Santa Cruz), IL-1β (1:500, #83186, Cell Signaling Technology), IL-18 (1:500, # DF6252, Affinity), LCN2 (1:500, #DF6816, Affinity), and GAPDH (1:10000, ab181602, Abcam).

    Techniques: Expressing, Western Blot, Activity Assay, Fluorescence, Immunohistochemical staining, Staining

    Calpeptin inhibits Calpain 1 activation and GSDMD cleavage in the tubules of IR mice, reduces cells apoptosis as well. (A,B) Representative immunofluorescence micrographs from mice kidneys of different groups stained Calpain 1 and GSDMD. ×400, bar = 50 μm. (C) Representative TUNEL staining micrographs in the mice kidneys of different groups. ×400, bar = 50 μm. (D) Quantitative determination of apoptosis cells amount. All Values were presented as mean ± SEM ( n = 3). *** p < 0.001 vs. sham group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion.

    Journal: Frontiers in Medicine

    Article Title: Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

    doi: 10.3389/fmed.2022.811980

    Figure Lengend Snippet: Calpeptin inhibits Calpain 1 activation and GSDMD cleavage in the tubules of IR mice, reduces cells apoptosis as well. (A,B) Representative immunofluorescence micrographs from mice kidneys of different groups stained Calpain 1 and GSDMD. ×400, bar = 50 μm. (C) Representative TUNEL staining micrographs in the mice kidneys of different groups. ×400, bar = 50 μm. (D) Quantitative determination of apoptosis cells amount. All Values were presented as mean ± SEM ( n = 3). *** p < 0.001 vs. sham group; ### p < 0.001 vs. IR group. CP, calpeptin; IR, ischemia/reperfusion.

    Article Snippet: The membrane was blocked by protein free rapid blocking buffer (PS108P, Epizyme) for 20 min and then incubated overnight with primary antibodies against Calpain 1 (1:1000, #2556, Cell Signaling Technology), Calpain 2 (1:1000, # DF7807, Affinity), Klotho (1:500, # DF10309, Affinity), AIM2(1:500, # DF3514, Affinity), NLRP3 (1:500, # DF7438, Affinity), pro-Caspase 1 (1:500, #24232, Cell Signaling Technology), cleaved-Caspase 1 (1:500, #89332, Cell Signaling Technology), ASC (1:1000, sc-514414, Santa Cruz), IL-1β (1:500, #83186, Cell Signaling Technology), IL-18 (1:500, # DF6252, Affinity), LCN2 (1:500, #DF6816, Affinity), and GAPDH (1:10000, ab181602, Abcam).

    Techniques: Activation Assay, Immunofluorescence, Staining, TUNEL Assay

    Calpeptin suppresses AIM2 and NLRP3 inflammasome signaling pathway, and increases Klotho protein expression in CoCl 2 -induced HK-2 cells hypoxia model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, ASC, IL-1β and LCN2 in the HK-2 cells. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1, IL-1β and LCN2. (C) The calpain activity of HK-2 cells was assessed by the relative fluorescence units (400/505 nm). (D) The cathepsin B activity of HK-2 cells was analyzed by the relative fluorescence units (400/505 nm). All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. control group; ** p < 0.01 vs. control group; *** p < 0.001 vs. control group; # p < 0.05 vs. CoCl 2 group; ## p < 0.01 vs. CoCl 2 group; ### p < 0.001 vs. CoCl 2 group. CP, calpeptin; RFU, relative fluorescence units.

    Journal: Frontiers in Medicine

    Article Title: Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein

    doi: 10.3389/fmed.2022.811980

    Figure Lengend Snippet: Calpeptin suppresses AIM2 and NLRP3 inflammasome signaling pathway, and increases Klotho protein expression in CoCl 2 -induced HK-2 cells hypoxia model. (A) Western blotting of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, pro-Caspase 1, cleaved-Caspase 1, ASC, IL-1β and LCN2 in the HK-2 cells. (B) Quantitative determination of Calpain 1, Calpain 2, Klotho, AIM2, NLRP3, cleaved-Caspase 1, IL-1β and LCN2. (C) The calpain activity of HK-2 cells was assessed by the relative fluorescence units (400/505 nm). (D) The cathepsin B activity of HK-2 cells was analyzed by the relative fluorescence units (400/505 nm). All data were presented as mean ± SEM ( n = 3). NS, no significance; * p < 0.05 vs. control group; ** p < 0.01 vs. control group; *** p < 0.001 vs. control group; # p < 0.05 vs. CoCl 2 group; ## p < 0.01 vs. CoCl 2 group; ### p < 0.001 vs. CoCl 2 group. CP, calpeptin; RFU, relative fluorescence units.

    Article Snippet: The membrane was blocked by protein free rapid blocking buffer (PS108P, Epizyme) for 20 min and then incubated overnight with primary antibodies against Calpain 1 (1:1000, #2556, Cell Signaling Technology), Calpain 2 (1:1000, # DF7807, Affinity), Klotho (1:500, # DF10309, Affinity), AIM2(1:500, # DF3514, Affinity), NLRP3 (1:500, # DF7438, Affinity), pro-Caspase 1 (1:500, #24232, Cell Signaling Technology), cleaved-Caspase 1 (1:500, #89332, Cell Signaling Technology), ASC (1:1000, sc-514414, Santa Cruz), IL-1β (1:500, #83186, Cell Signaling Technology), IL-18 (1:500, # DF6252, Affinity), LCN2 (1:500, #DF6816, Affinity), and GAPDH (1:10000, ab181602, Abcam).

    Techniques: Expressing, Western Blot, Activity Assay, Fluorescence