antibodies against phospho ampkα  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against phospho ampkα
    Antibodies Against Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibodies against phospho ampkα  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against phospho ampkα
    Antibodies Against Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against phospho ampkα/product/Cell Signaling Technology Inc
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    phospho ampkα  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho ampkα
    Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampkα/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    phospho ampk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho ampk
    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against <t>pmTor,</t> <t>T-mTor,</t> <t>p-Ampk,</t> T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
    Phospho Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampk/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho ampk - by Bioz Stars, 2023-01
    99/100 stars

    Images

    1) Product Images from "The Mechanism of Action of the Histone Deacetylase Inhibitor Vorinostat Involves Interaction with the Insulin-Like Growth Factor Signaling Pathway"

    Article Title: The Mechanism of Action of the Histone Deacetylase Inhibitor Vorinostat Involves Interaction with the Insulin-Like Growth Factor Signaling Pathway

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0024468

    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against pmTor, T-mTor, p-Ampk, T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
    Figure Legend Snippet: Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against pmTor, T-mTor, p-Ampk, T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.

    Techniques Used: Western Blot

    anti phospho ampkα thr172 ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho ampkα thr172 ab
    Anti Phospho Ampkα Thr172 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr172 ab/product/Cell Signaling Technology Inc
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    anti phospho ampkα thr172 ab - by Bioz Stars, 2023-01
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    anti phospho ampkα thr172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho ampkα thr172
    Anti Phospho Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr172/product/Cell Signaling Technology Inc
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    anti phospho ampkα thr172 - by Bioz Stars, 2023-01
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    phospho ampkα  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc phospho ampkα
    Total RNA and protein were extracted from liver tissue and (A) Nampt <t>,</t> <t>Sirt1</t> , Lkb1 , AMPK α1 and AMPK α1 mRNA and (B) GLP-1R, Sirt1, <t>phospho-AMPKα,</t> AMPK and β-actin protein expression were measured by quantitative real-time RT-PCR and western blot, respectively. Data were normalized to the β-actin of each sample. All values are expressed as the mean ± SE for n = 5–7 mice. * p <0.05, ** p <0.01 compared with a control and # p <0.05, ## p <0.01 compared with a HF.
    Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampkα/product/Cell Signaling Technology Inc
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    phospho ampkα - by Bioz Stars, 2023-01
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    1) Product Images from "Exendin-4 Improves Steatohepatitis by Increasing Sirt1 Expression in High-Fat Diet-Induced Obese C57BL/6J Mice"

    Article Title: Exendin-4 Improves Steatohepatitis by Increasing Sirt1 Expression in High-Fat Diet-Induced Obese C57BL/6J Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0031394

    Total RNA and protein were extracted from liver tissue and (A) Nampt , Sirt1 , Lkb1 , AMPK α1 and AMPK α1 mRNA and (B) GLP-1R, Sirt1, phospho-AMPKα, AMPK and β-actin protein expression were measured by quantitative real-time RT-PCR and western blot, respectively. Data were normalized to the β-actin of each sample. All values are expressed as the mean ± SE for n = 5–7 mice. * p <0.05, ** p <0.01 compared with a control and # p <0.05, ## p <0.01 compared with a HF.
    Figure Legend Snippet: Total RNA and protein were extracted from liver tissue and (A) Nampt , Sirt1 , Lkb1 , AMPK α1 and AMPK α1 mRNA and (B) GLP-1R, Sirt1, phospho-AMPKα, AMPK and β-actin protein expression were measured by quantitative real-time RT-PCR and western blot, respectively. Data were normalized to the β-actin of each sample. All values are expressed as the mean ± SE for n = 5–7 mice. * p <0.05, ** p <0.01 compared with a control and # p <0.05, ## p <0.01 compared with a HF.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    (A) Cells were treated with 50 nM, 100 nM, or 500 nM Ex-4 for 24 h. GLP-1R and β-actin were measured by western blot and real time PCR. GLP-1R was normalized to β-actin. (B) Cells given 0.4 mM palmitic acid (PA) were treated with either vehicle or 50 nM to 100 nM exendin-4 for 24 h. (C) Cells given 0.4 mM palmitic acid were treated with 100 nM exendin-4 in the absence or presence of 10 mM nicotinamice (NAM) or 10 uM compound C (CC) for 24 h. (B–C) Sirt1, phosphorylated AMPKα at threonine 172, AMPK and β-actin were measured by western blot in HepG2 cells. Sirt1 and phosphorylated AMPKα were normalized to the β-actin and total AMPK of each sample, respectively. * p <0.05, ** p <0.01 compared with control, # p <0.05, ## p <0.01 compared with PA, and † p <0.05, †† p <0.01 compared with Ex-4.
    Figure Legend Snippet: (A) Cells were treated with 50 nM, 100 nM, or 500 nM Ex-4 for 24 h. GLP-1R and β-actin were measured by western blot and real time PCR. GLP-1R was normalized to β-actin. (B) Cells given 0.4 mM palmitic acid (PA) were treated with either vehicle or 50 nM to 100 nM exendin-4 for 24 h. (C) Cells given 0.4 mM palmitic acid were treated with 100 nM exendin-4 in the absence or presence of 10 mM nicotinamice (NAM) or 10 uM compound C (CC) for 24 h. (B–C) Sirt1, phosphorylated AMPKα at threonine 172, AMPK and β-actin were measured by western blot in HepG2 cells. Sirt1 and phosphorylated AMPKα were normalized to the β-actin and total AMPK of each sample, respectively. * p <0.05, ** p <0.01 compared with control, # p <0.05, ## p <0.01 compared with PA, and † p <0.05, †† p <0.01 compared with Ex-4.

    Techniques Used: Western Blot, Real-time Polymerase Chain Reaction

    anti phospho ampkα thr 172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho ampkα thr 172
    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK <t>Thr</t> <t>172</t> ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Anti Phospho Ampkα Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr 172/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti phospho ampkα thr 172 - by Bioz Stars, 2023-01
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    1) Product Images from "The Metabolic Consequences of Hepatic AMP-Kinase Phosphorylation in Rainbow Trout"

    Article Title: The Metabolic Consequences of Hepatic AMP-Kinase Phosphorylation in Rainbow Trout

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0020228

    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Figure Legend Snippet: Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Techniques Used: Injection

    Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. mRNA levels were estimated using real-time RT-PCR. Expression levels were normalized to β-actin-expressed transcripts which did not change under the experimental conditions and are presented as fold-change against the saline solution-treated group set to 1. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Figure Legend Snippet: Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. mRNA levels were estimated using real-time RT-PCR. Expression levels were normalized to β-actin-expressed transcripts which did not change under the experimental conditions and are presented as fold-change against the saline solution-treated group set to 1. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Techniques Used: Quantitative RT-PCR, Expressing

    anti ampkα thr172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ampkα thr172
    Anti Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho ampk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho ampk
    (A) Analysis of phospho-αAMPK, total α and β <t>AMPK</t> expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-AMPK to AMPK ratio for western-blot expressed as mean ± SEM. (B) Analysis <t>of</t> <t>phospho-ACC</t> and total ACC expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-ACC to ACC ratio for western-blot expressed as mean ± SEM. Data are presented with WT set to one.
    Phospho Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampk/product/Cell Signaling Technology Inc
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    phospho ampk - by Bioz Stars, 2023-01
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    1) Product Images from "Absence of RIP140 Reveals a Pathway Regulating glut4-Dependent Glucose Uptake in Oxidative Skeletal Muscle through UCP1-Mediated Activation of AMPK"

    Article Title: Absence of RIP140 Reveals a Pathway Regulating glut4-Dependent Glucose Uptake in Oxidative Skeletal Muscle through UCP1-Mediated Activation of AMPK

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032520

    (A) Analysis of phospho-αAMPK, total α and β AMPK expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-AMPK to AMPK ratio for western-blot expressed as mean ± SEM. (B) Analysis of phospho-ACC and total ACC expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-ACC to ACC ratio for western-blot expressed as mean ± SEM. Data are presented with WT set to one.
    Figure Legend Snippet: (A) Analysis of phospho-αAMPK, total α and β AMPK expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-AMPK to AMPK ratio for western-blot expressed as mean ± SEM. (B) Analysis of phospho-ACC and total ACC expression on RIP140-null (KO), transgenic (TG) and WT soleus (SOL) by western-blot. Quantification of phospho-ACC to ACC ratio for western-blot expressed as mean ± SEM. Data are presented with WT set to one.

    Techniques Used: Expressing, Transgenic Assay, Western Blot

    Absence of RIP140 leads to expression of UCP1 and mitochondrial uncoupling. Then, alteration of the AMP to ATP ratio in favor of elevated AMP activates AMPK which in turn stimulates GLUT4 translocation to the plasma membrane enabling the entry of glucose from blood circulation.
    Figure Legend Snippet: Absence of RIP140 leads to expression of UCP1 and mitochondrial uncoupling. Then, alteration of the AMP to ATP ratio in favor of elevated AMP activates AMPK which in turn stimulates GLUT4 translocation to the plasma membrane enabling the entry of glucose from blood circulation.

    Techniques Used: Expressing, Translocation Assay

    phospho ampkα thr172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho ampkα thr172
    Phospho Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc antibodies against phospho ampkα
    Antibodies Against Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Phospho Ampkα, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against <t>pmTor,</t> <t>T-mTor,</t> <t>p-Ampk,</t> T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
    Phospho Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampk/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti phospho ampkα thr172 ab
    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against <t>pmTor,</t> <t>T-mTor,</t> <t>p-Ampk,</t> T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
    Anti Phospho Ampkα Thr172 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr172 ab/product/Cell Signaling Technology Inc
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    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against <t>pmTor,</t> <t>T-mTor,</t> <t>p-Ampk,</t> T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.
    Anti Phospho Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr172/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    Cell Signaling Technology Inc anti phospho ampkα thr 172
    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK <t>Thr</t> <t>172</t> ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Anti Phospho Ampkα Thr 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho ampkα thr 172/product/Cell Signaling Technology Inc
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    anti phospho ampkα thr 172 - by Bioz Stars, 2023-01
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    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK <t>Thr</t> <t>172</t> ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Anti Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ampkα thr172/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti ampkα thr172 - by Bioz Stars, 2023-01
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    Cell Signaling Technology Inc phospho ampkα thr172
    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK <t>Thr</t> <t>172</t> ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).
    Phospho Ampkα Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ampkα thr172/product/Cell Signaling Technology Inc
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    phospho ampkα thr172 - by Bioz Stars, 2023-01
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    Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against pmTor, T-mTor, p-Ampk, T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.

    Journal: PLoS ONE

    Article Title: The Mechanism of Action of the Histone Deacetylase Inhibitor Vorinostat Involves Interaction with the Insulin-Like Growth Factor Signaling Pathway

    doi: 10.1371/journal.pone.0024468

    Figure Lengend Snippet: Ishikawa and USPC-2 cells were treated with vorinostat and/or IGF-I, and cell lysates (100 µg) were prepared after 24 h. Western blot analysis was performed with antibodies against pmTor, T-mTor, p-Ampk, T-Ampk, p21, cyclin D1, PI3K and actin. The figure shows the results of a typical experiment, repeated three times with similar results.

    Article Snippet: Antibodies against phospho-IGF-IR (3024), IGF-IR β-subunit (3027), insulin receptor [(IR); 3025], phospho-AKT (9271), AKT (9272), phospho-ERK1/2 (9106), poly ADP ribose polymerase [(PARP); 9542], caspase 3 (9661), phospho-Ampk (2531), Ampk (2532), phospho-mTor (5536), mTor (2983), and PI3K p85 (4292) were obtained from Cell Signaling Technology.

    Techniques: Western Blot

    Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Journal: PLoS ONE

    Article Title: The Metabolic Consequences of Hepatic AMP-Kinase Phosphorylation in Rainbow Trout

    doi: 10.1371/journal.pone.0020228

    Figure Lengend Snippet: Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Article Snippet: Protein extraction (20 µg) and Western blotting were undertaken as in using anti-phospho-AMPKα Thr 172 and anti-AMPK antibodies (Cell Signaling Technology, France) that recognize both α-1 and α-2 isoforms of the enzyme.

    Techniques: Injection

    Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. mRNA levels were estimated using real-time RT-PCR. Expression levels were normalized to β-actin-expressed transcripts which did not change under the experimental conditions and are presented as fold-change against the saline solution-treated group set to 1. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Journal: PLoS ONE

    Article Title: The Metabolic Consequences of Hepatic AMP-Kinase Phosphorylation in Rainbow Trout

    doi: 10.1371/journal.pone.0020228

    Figure Lengend Snippet: Gels were loaded with 20 µg total protein per lane. Protein (total AMPK) and phosphorylation levels (AMPK Thr 172 ) were normalized to tissue β-tubulin levels. mRNA levels were estimated using real-time RT-PCR. Expression levels were normalized to β-actin-expressed transcripts which did not change under the experimental conditions and are presented as fold-change against the saline solution-treated group set to 1. Results are expressed as means ± s.e.m. ( n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference ( α <0.05).

    Article Snippet: Protein extraction (20 µg) and Western blotting were undertaken as in using anti-phospho-AMPKα Thr 172 and anti-AMPK antibodies (Cell Signaling Technology, France) that recognize both α-1 and α-2 isoforms of the enzyme.

    Techniques: Quantitative RT-PCR, Expressing