anti early endosome antigen 1 eea 1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti early endosome antigen 1 eea 1 antibody
    Anti Early Endosome Antigen 1 Eea 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti early endosome antigen 1 eea 1 antibody/product/Cell Signaling Technology Inc
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    anti early endosome antigen 1 eea 1 antibody - by Bioz Stars, 2023-02
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    anti early endosome antigen 1 eea 1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti early endosome antigen 1 eea 1 antibody
    Anti Early Endosome Antigen 1 Eea 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti early endosome antigen 1 eea 1 antibody/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
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    anti early endosome antigen 1 eea 1 antibody - by Bioz Stars, 2023-02
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    rabbit anti eea1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti eea1
    (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, <t>EEA1</t> and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.
    Rabbit Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti eea1/product/Cell Signaling Technology Inc
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    rabbit anti eea1 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Inhibition of PIKfyve by YM-201636 Dysregulates Autophagy and Leads to Apoptosis-Independent Neuronal Cell Death"

    Article Title: Inhibition of PIKfyve by YM-201636 Dysregulates Autophagy and Leads to Apoptosis-Independent Neuronal Cell Death

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0060152

    (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, EEA1 and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.
    Figure Legend Snippet: (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, EEA1 and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.

    Techniques Used: Electron Microscopy

    Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h, then supplemented with 5 µg/ml WGA-Alexa555 for the final 5 min (A,B) or 30 min (C,D). Cells were fixed, immunolabelled for EEA1 and LAMP1, and imaged by confocal microscopy. (B,D) The intensity of WGA-Alexa555 per µm 2 in the cell body and the total area of the cell body were determined in the YM-201636–treated cells relative to DMSO. (E,F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 in the presence of 5 µg/ml WGA-Alexa555 for the full 4 h. Cells were fixed, immunolabelled for GM130 and imaged by confocal microscopy. (F) The amount of WGA-Alexa555 fluorescent intensity in the cell body/µm 2 of the YM-2016363 treated cells was determined relative to DMSO. The area of the cell bodies analysed was also determined (mean ± SEM, n = 3 independent experiments, 9–28 cells per experiment). Significances relative to DMSO **p<0.01. Scale bar = 10 µm.
    Figure Legend Snippet: Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h, then supplemented with 5 µg/ml WGA-Alexa555 for the final 5 min (A,B) or 30 min (C,D). Cells were fixed, immunolabelled for EEA1 and LAMP1, and imaged by confocal microscopy. (B,D) The intensity of WGA-Alexa555 per µm 2 in the cell body and the total area of the cell body were determined in the YM-201636–treated cells relative to DMSO. (E,F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 in the presence of 5 µg/ml WGA-Alexa555 for the full 4 h. Cells were fixed, immunolabelled for GM130 and imaged by confocal microscopy. (F) The amount of WGA-Alexa555 fluorescent intensity in the cell body/µm 2 of the YM-2016363 treated cells was determined relative to DMSO. The area of the cell bodies analysed was also determined (mean ± SEM, n = 3 independent experiments, 9–28 cells per experiment). Significances relative to DMSO **p<0.01. Scale bar = 10 µm.

    Techniques Used: Confocal Microscopy

    rabbit anti eea1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti eea1 antibody
    BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker <t>EEA1</t> (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.
    Rabbit Anti Eea1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti eea1 antibody/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
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    rabbit anti eea1 antibody - by Bioz Stars, 2023-02
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    1) Product Images from "Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro"

    Article Title: Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro

    Journal: Theranostics

    doi: 10.7150/thno.20746

    BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker EEA1 (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.
    Figure Legend Snippet: BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker EEA1 (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.

    Techniques Used: Derivative Assay, Incubation, Confocal Microscopy, Marker

    polyclonal rabbit anti eea1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit anti eea1
    Polyclonal Rabbit Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti eea1/product/Cell Signaling Technology Inc
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    polyclonal rabbit anti eea1 - by Bioz Stars, 2023-02
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    anti eea1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti eea1
    Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), <t>EEA1</t> for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.
    Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti eea1/product/Cell Signaling Technology Inc
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    1) Product Images from "Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles"

    Article Title: Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles

    Journal: Journal of Extracellular Vesicles

    doi: 10.1080/20013078.2017.1354645

    Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), EEA1 for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.
    Figure Legend Snippet: Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), EEA1 for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.

    Techniques Used: Incubation, Confocal Microscopy, Software

    eea 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc eea 1
    Eea 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    eea 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc eea 1
    Eea 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal rabbit anti human eea1 immunoglobulin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit anti human eea1 immunoglobulin
    Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for <t>EEA1</t> and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).
    Polyclonal Rabbit Anti Human Eea1 Immunoglobulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti human eea1 immunoglobulin/product/Cell Signaling Technology Inc
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    polyclonal rabbit anti human eea1 immunoglobulin - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge"

    Article Title: Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/1477-3155-10-28

    Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for EEA1 and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).
    Figure Legend Snippet: Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for EEA1 and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).

    Techniques Used: Incubation, Concentration Assay, Staining, Fluorescence

    anti early endosome antigen 1 eea 1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti early endosome antigen 1 eea 1 antibody
    Anti Early Endosome Antigen 1 Eea 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti early endosome antigen 1 eea 1 antibody/product/Cell Signaling Technology Inc
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    rabbit derived monoclonal antibody against eea1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit derived monoclonal antibody against eea1
    GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). <t>EEA1,</t> Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.
    Rabbit Derived Monoclonal Antibody Against Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit derived monoclonal antibody against eea1/product/Cell Signaling Technology Inc
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    1) Product Images from "GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus"

    Article Title: GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2019.1677446

    GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). EEA1, Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.
    Figure Legend Snippet: GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). EEA1, Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.

    Techniques Used: Expressing, Western Blot, Staining

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    Cell Signaling Technology Inc anti early endosome antigen 1 eea 1 antibody
    Anti Early Endosome Antigen 1 Eea 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti early endosome antigen 1 eea 1 antibody/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc rabbit anti eea1
    (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, <t>EEA1</t> and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.
    Rabbit Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit anti eea1 antibody
    BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker <t>EEA1</t> (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.
    Rabbit Anti Eea1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti eea1 antibody/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc polyclonal rabbit anti eea1
    BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker <t>EEA1</t> (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.
    Polyclonal Rabbit Anti Eea1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), <t>EEA1</t> for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.
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    Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for <t>EEA1</t> and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).
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    GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). <t>EEA1,</t> Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.
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    Image Search Results


    (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, EEA1 and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.

    Journal: PLoS ONE

    Article Title: Inhibition of PIKfyve by YM-201636 Dysregulates Autophagy and Leads to Apoptosis-Independent Neuronal Cell Death

    doi: 10.1371/journal.pone.0060152

    Figure Lengend Snippet: (A) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h and processed for electron microscopy. Images show neuronal cell bodies at the level of the nucleus, demonstrating vacuolation in the presence of YM-201636. Grid size = 1 µm square. (B-D) Electron microscopic analysis of the vacuole area as a percentage of total cytoplasmic area (B) and the number of vacuoles per cell (C) shows a significant increase in vacuole size and number following 4 h treatment with 1 µM YM-201636, **p<0.01, ***p<0.001. (D) Histogram of number of vacuoles relative to their size (representative experiment), n = 12 (DMSO) or 11 (YM-201636) cells. (E) Examples of vacuole phenotypes detected in primary hippocampal neurons treated with 1 µM YM-201636 for 4 h. (F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h or 18 h and immunolabelled for LAMP1, EEA1 and ß3-tubulin. 3D projections are shown. Scale bar = 10 µm.

    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-LC3 (Novus Biologicals, NB100-2331), mouse anti-ß-actin (Sigma, S0644), mouse anti-ßIII tubulin (Covance, MMS-435P), rabbit anti-EEA1 (#2411) and rabbit anti-cleaved caspase-3 (#9661) (Cell Signaling Technology), mouse anti-LAMP1/LY1C6 (Sapphire Bioscience, #120-13523), rat anti-LAMP1/CD107a (#553792), and mouse anti-GM130 (#610822) (BD Biosciences).

    Techniques: Electron Microscopy

    Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h, then supplemented with 5 µg/ml WGA-Alexa555 for the final 5 min (A,B) or 30 min (C,D). Cells were fixed, immunolabelled for EEA1 and LAMP1, and imaged by confocal microscopy. (B,D) The intensity of WGA-Alexa555 per µm 2 in the cell body and the total area of the cell body were determined in the YM-201636–treated cells relative to DMSO. (E,F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 in the presence of 5 µg/ml WGA-Alexa555 for the full 4 h. Cells were fixed, immunolabelled for GM130 and imaged by confocal microscopy. (F) The amount of WGA-Alexa555 fluorescent intensity in the cell body/µm 2 of the YM-2016363 treated cells was determined relative to DMSO. The area of the cell bodies analysed was also determined (mean ± SEM, n = 3 independent experiments, 9–28 cells per experiment). Significances relative to DMSO **p<0.01. Scale bar = 10 µm.

    Journal: PLoS ONE

    Article Title: Inhibition of PIKfyve by YM-201636 Dysregulates Autophagy and Leads to Apoptosis-Independent Neuronal Cell Death

    doi: 10.1371/journal.pone.0060152

    Figure Lengend Snippet: Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 for 4 h, then supplemented with 5 µg/ml WGA-Alexa555 for the final 5 min (A,B) or 30 min (C,D). Cells were fixed, immunolabelled for EEA1 and LAMP1, and imaged by confocal microscopy. (B,D) The intensity of WGA-Alexa555 per µm 2 in the cell body and the total area of the cell body were determined in the YM-201636–treated cells relative to DMSO. (E,F) Primary hippocampal neurons were treated with DMSO or 1 µM YM-201636 in the presence of 5 µg/ml WGA-Alexa555 for the full 4 h. Cells were fixed, immunolabelled for GM130 and imaged by confocal microscopy. (F) The amount of WGA-Alexa555 fluorescent intensity in the cell body/µm 2 of the YM-2016363 treated cells was determined relative to DMSO. The area of the cell bodies analysed was also determined (mean ± SEM, n = 3 independent experiments, 9–28 cells per experiment). Significances relative to DMSO **p<0.01. Scale bar = 10 µm.

    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-LC3 (Novus Biologicals, NB100-2331), mouse anti-ß-actin (Sigma, S0644), mouse anti-ßIII tubulin (Covance, MMS-435P), rabbit anti-EEA1 (#2411) and rabbit anti-cleaved caspase-3 (#9661) (Cell Signaling Technology), mouse anti-LAMP1/LY1C6 (Sapphire Bioscience, #120-13523), rat anti-LAMP1/CD107a (#553792), and mouse anti-GM130 (#610822) (BD Biosciences).

    Techniques: Confocal Microscopy

    BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker EEA1 (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.

    Journal: Theranostics

    Article Title: Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro

    doi: 10.7150/thno.20746

    Figure Lengend Snippet: BMMSC-EVs are taken up by chondrocytes derived from OA patients. CFSE labelled EVs derived from TERT-bone marrow MSC were incubated for 30 min with OA chondrocytes and the uptake of EVs was analyzed by confocal microscopy. Left panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with late endosomal marker LAMP1 (red) in OA chondrocytes are shown. Arrow heads indicate the co-localization (yellow) of BMMSC-EVs with LAMP1. Right panel: Representative images of co-localization analyses of CFSE labelled (green) BMMSC-EVs with early endosomal marker EEA1 (red) in OA chondrocytes is shown. Images are representative of 3 independent experiments. Scale bar is 10 µm.

    Article Snippet: For BMMSC-EV uptake analysis, fixed OA chondrocytes were incubated with 2% BSA for 30 min followed by 1 h incubation with a rabbit anti-EEA1 antibody (1:200; Cell Signaling) and mouse anti-LAMP-1 (1:400, BD Pharmigen) followed by subsequent incubation with a donkey-anti-rabbit antibody labelled with DyLight 549 (Jackson) and a goat-anti-mouse antibody labelled with DyLight 649 (Jackson), respectively.

    Techniques: Derivative Assay, Incubation, Confocal Microscopy, Marker

    Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), EEA1 for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.

    Journal: Journal of Extracellular Vesicles

    Article Title: Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles

    doi: 10.1080/20013078.2017.1354645

    Figure Lengend Snippet: Localisation of the EV-associated DiIC 18 (5)-DS label in prostate cells. ( a ) Cells were incubated for 16 h with PC-3 and PNT2 DiIC 18 (5)-DS labelled EVs (DiIC 18 -EVs) (pseudo-coloured green). Cells were immunostained with antibodies against CD44 for the localisation of plasma membrane (PM), EEA1 for early endosomes (EE), Lamp1 for lysosomes (LYS), and GM130 for Golgi (red), and imaged by confocal microscopy. Scale bars 10 µm. Insets show a zoom into the organelle. ( b ) The percentage of the DiIC 18 dye co-localisation with each antibody was quantified by Imaris software ( n = 42–110 cells). Error bars represent the mean ± SEM of 8–36 image frames each, * p < 0.05 unpaired t-test.

    Article Snippet: Samples were stained at 4°C overnight with anti-CD44 (1:200, gift from Dr Jalkanen, Turku, Finland), anti-Lamp1 (H4A3, 1:100, IOWA University, Developmental Studies, USA), polyclonal anti-GM130 (1:100) or anti-EEA1 (1:100) (Cell Signaling, Danvers, MA, USA).

    Techniques: Incubation, Confocal Microscopy, Software

    Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for EEA1 and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).

    Journal: Journal of Nanobiotechnology

    Article Title: Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge

    doi: 10.1186/1477-3155-10-28

    Figure Lengend Snippet: Intracellular localization of SPIONs. Cells were incubated concomitantly with both SPIONs (γ-Fe 2 O 3 -PEI-FITC and γ-Fe 2 O 3 -PMA-Dy636) at a final concentration of 1 μg/ml [Fe] for time periods of 30 min, 1 h, 2 h, 4 h, 8 h, and 24 h. Cells were then stained with wheat germ agglutinin, for EEA1 and for LAMP-1 to visualize the Plasma Membrane (white), the Endosomes (yellow) and the Lysosomes (red), respectively. Fluorescence images were recorded with a LSM. Additionally, the same experiments were performed with cells incubated with each NP system (see ).

    Article Snippet: Lysosomes were stained using monoclonal mouse anti-human LAMP1/CD107a antibodies (Developmental Studies Hybridoma Bank), while early endosomes were labeled with polyclonal rabbit anti-human EEA1 immunoglobulin (Cell Signaling).

    Techniques: Incubation, Concentration Assay, Staining, Fluorescence

    GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). EEA1, Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.

    Journal: Emerging Microbes & Infections

    Article Title: GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus

    doi: 10.1080/22221751.2019.1677446

    Figure Lengend Snippet: GILT expression is upregulated by IFN-γ in lung cancer cells and has a lysosomal distribution. A549 and MRC-5 cells were treated with the indicated concentrations of IFN-α or IFN-γ for 12 h. (A) The levels of intracellular GILT and IFITM proteins were determined by Western blot assay. β-actin served as a loading control. (B) The localization of GILT in MRC-5 cells treated with IFN-γ was detected by immunofluorescent staining with anti-GILT polyclonal antibody (green). EEA1, Rab9 or LAMP1 were visualized by immunofluorescent staining with respective antibodies (red). Cell nuclei were stained blue with DAPI.

    Article Snippet: To visualize the subcellular localization of wild-type and mutant GILT proteins, A549 or TRex 293 cells were first fixed with 4% paraformaldehyde for 20 min and then permeabilized with 0.1% Triton X-100 for 10 min. After incubation in the blocking solution (1×PBS with 3% BSA and 5% FBS) for 1 h, cells were stained with a mouse monoclonal antibody (G-11, SANTA CRUZ sc-393507A) or rabbit polyclonal antibody recognizing GILT protein (sc-393507A) together with a Rabbit derived monoclonal antibody against EEA1 (Cell Signaling, 2411), Rab9 (Cell Signaling, 5118s) or mouse derived LAMP1 monoclonal antibody (Cell Signaling, 15665), respectively.

    Techniques: Expressing, Western Blot, Staining