anti ap2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ap2
    The primer sequences used for real-time PCR.
    Anti Ap2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ap2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ap2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells"

    Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2013/475386

    The primer sequences used for real-time PCR.
    Figure Legend Snippet: The primer sequences used for real-time PCR.

    Techniques Used:

    Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.
    Figure Legend Snippet: Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    anti ap2  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc anti ap2
    The primer sequences used for real-time PCR.
    Anti Ap2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ap2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ap2 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells"

    Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2013/475386

    The primer sequences used for real-time PCR.
    Figure Legend Snippet: The primer sequences used for real-time PCR.

    Techniques Used:

    Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.
    Figure Legend Snippet: Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    tfap2c  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tfap2c
    (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for <t>TFAP2C</t> (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .
    Tfap2c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfap2c/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    tfap2c - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Coordination Between Embryo Growth and Trophoblast Migration Upon Implantation Delineates Mouse Embryogenesis"

    Article Title: Coordination Between Embryo Growth and Trophoblast Migration Upon Implantation Delineates Mouse Embryogenesis

    Journal: bioRxiv

    doi: 10.1101/2022.06.13.495767

    (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .
    Figure Legend Snippet: (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .

    Techniques Used: Immunofluorescence, Staining, In Utero, Embryo Culture, In Vitro, MANN-WHITNEY, Immunostaining

    tfap2c  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tfap2c
    (a) Expression of trophoblast markers as measured by RT-PCR in pdTSCs that were maintained for prolonged time (10-15 Passages) in TSC-m medium, or in primed hPSCs, compared to native TSC lines bTS5 and CT30. (n=3) (b) Immunostaining of trophoblast markers CK7, GATA3 and <t>TFAP2C</t> in cells that were maintained for prolonged time (15 Passages) in TSC-m medium. (c) FACS analysis of H1, WIBR3 and JHiPS pdTSCs, measuring anti-ITGA6 or IgG (negative control), after 15 passages in TSC-m medium. (d) Methylation levels of ELF5 promoter, as measured in primed hPSC (left) and in pdTSC (right). (e) Karyotype analysis for H1, JHiPS and WIBR3 pdTSCs.
    Tfap2c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Human primed and naïve PSCs are both competent in differentiating into bona fide trophoblast stem cells"

    Article Title: Human primed and naïve PSCs are both competent in differentiating into bona fide trophoblast stem cells

    Journal: bioRxiv

    doi: 10.1101/2022.05.20.492766

    (a) Expression of trophoblast markers as measured by RT-PCR in pdTSCs that were maintained for prolonged time (10-15 Passages) in TSC-m medium, or in primed hPSCs, compared to native TSC lines bTS5 and CT30. (n=3) (b) Immunostaining of trophoblast markers CK7, GATA3 and TFAP2C in cells that were maintained for prolonged time (15 Passages) in TSC-m medium. (c) FACS analysis of H1, WIBR3 and JHiPS pdTSCs, measuring anti-ITGA6 or IgG (negative control), after 15 passages in TSC-m medium. (d) Methylation levels of ELF5 promoter, as measured in primed hPSC (left) and in pdTSC (right). (e) Karyotype analysis for H1, JHiPS and WIBR3 pdTSCs.
    Figure Legend Snippet: (a) Expression of trophoblast markers as measured by RT-PCR in pdTSCs that were maintained for prolonged time (10-15 Passages) in TSC-m medium, or in primed hPSCs, compared to native TSC lines bTS5 and CT30. (n=3) (b) Immunostaining of trophoblast markers CK7, GATA3 and TFAP2C in cells that were maintained for prolonged time (15 Passages) in TSC-m medium. (c) FACS analysis of H1, WIBR3 and JHiPS pdTSCs, measuring anti-ITGA6 or IgG (negative control), after 15 passages in TSC-m medium. (d) Methylation levels of ELF5 promoter, as measured in primed hPSC (left) and in pdTSC (right). (e) Karyotype analysis for H1, JHiPS and WIBR3 pdTSCs.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunostaining, Negative Control, Methylation

    rabbit anti tfap2c  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti tfap2c
    Rabbit Anti Tfap2c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tfap2c/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit anti tfap2c - by Bioz Stars, 2023-01
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    anti ap2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ap2
    Anti Ap2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ap2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    anti ap 2γ  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti ap 2γ

    Anti Ap 2γ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ap 2γ/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti ap 2γ - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures"

    Article Title: High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

    Journal: eLife

    doi: 10.7554/eLife.59445


    Figure Legend Snippet:

    Techniques Used: Recombinant, Multiplex Assay, Software

    tfap2c  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tfap2c
    Expression analysis of key genes involved in PGC specification. (A,B) Immunofluorescence of day 4 embryoids showing co-expression of <t>TFAP2C</t> with BLIMP1, and OCT4 with SOX17. Scale bars, 100 μm. (C) Box plots for the percentage of BLIMP1/TFAP2C (left), and SOX17/OCT4 (right) double-positive cells in day 4 embryoids differentiated from the NOA iPSCs, normal hiPSCs, and hESCs. The quantification of double-positive cells was performed for at least six independent embryoids. Black central line represents the median; boxes represent the 25th and 75th percentiles, and whiskers represent the maximum and minimum, respectively. (D) Relative expression levels of PGC-related genes in hiPSCs/hESCs before PGC induction (day -2) and in day 4 embryoids were measured by qRT-PCR and shown with normalization to housekeeping gene GAPDH. Error bars indicate mean ± SD of three independent experiments. Asterisk indicated statistically significant differences ( P < 0.05) between the NOA iPSCs and the normal iPSCs or H1 ESCs.
    Tfap2c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfap2c/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tfap2c - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Induced Pluripotent Stem Cells Derived From Two Idiopathic Azoospermia Patients Display Compromised Differentiation Potential for Primordial Germ Cell Fate"

    Article Title: Induced Pluripotent Stem Cells Derived From Two Idiopathic Azoospermia Patients Display Compromised Differentiation Potential for Primordial Germ Cell Fate

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.00432

    Expression analysis of key genes involved in PGC specification. (A,B) Immunofluorescence of day 4 embryoids showing co-expression of TFAP2C with BLIMP1, and OCT4 with SOX17. Scale bars, 100 μm. (C) Box plots for the percentage of BLIMP1/TFAP2C (left), and SOX17/OCT4 (right) double-positive cells in day 4 embryoids differentiated from the NOA iPSCs, normal hiPSCs, and hESCs. The quantification of double-positive cells was performed for at least six independent embryoids. Black central line represents the median; boxes represent the 25th and 75th percentiles, and whiskers represent the maximum and minimum, respectively. (D) Relative expression levels of PGC-related genes in hiPSCs/hESCs before PGC induction (day -2) and in day 4 embryoids were measured by qRT-PCR and shown with normalization to housekeeping gene GAPDH. Error bars indicate mean ± SD of three independent experiments. Asterisk indicated statistically significant differences ( P < 0.05) between the NOA iPSCs and the normal iPSCs or H1 ESCs.
    Figure Legend Snippet: Expression analysis of key genes involved in PGC specification. (A,B) Immunofluorescence of day 4 embryoids showing co-expression of TFAP2C with BLIMP1, and OCT4 with SOX17. Scale bars, 100 μm. (C) Box plots for the percentage of BLIMP1/TFAP2C (left), and SOX17/OCT4 (right) double-positive cells in day 4 embryoids differentiated from the NOA iPSCs, normal hiPSCs, and hESCs. The quantification of double-positive cells was performed for at least six independent embryoids. Black central line represents the median; boxes represent the 25th and 75th percentiles, and whiskers represent the maximum and minimum, respectively. (D) Relative expression levels of PGC-related genes in hiPSCs/hESCs before PGC induction (day -2) and in day 4 embryoids were measured by qRT-PCR and shown with normalization to housekeeping gene GAPDH. Error bars indicate mean ± SD of three independent experiments. Asterisk indicated statistically significant differences ( P < 0.05) between the NOA iPSCs and the normal iPSCs or H1 ESCs.

    Techniques Used: Expressing, Immunofluorescence, Quantitative RT-PCR

    ap 2γ  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ap 2γ
    KEY RESOURCES TABLE
    Ap 2γ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap 2γ/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ap 2γ - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment"

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    Journal: Cell stem cell

    doi: 10.1016/j.stem.2018.12.012

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

    ap 2γ antibody for if  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ap 2γ antibody for if
    KEY RESOURCES TABLE
    Ap 2γ Antibody For If, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment"

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    Journal: Cell stem cell

    doi: 10.1016/j.stem.2018.12.012

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

    2320s rrid ab 10695101  (Cell Signaling Technology Inc)


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  • 94

    Structured Review

    Cell Signaling Technology Inc 2320s rrid ab 10695101
    KEY RESOURCES TABLE
    2320s Rrid Ab 10695101, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2320s rrid ab 10695101/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Images

    1) Product Images from "TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment"

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    Journal: Cell stem cell

    doi: 10.1016/j.stem.2018.12.012

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

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    Cell Signaling Technology Inc anti ap2
    The primer sequences used for real-time PCR.
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    (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for <t>TFAP2C</t> (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .
    Tfap2c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for <t>TFAP2C</t> (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .
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    Image Search Results


    The primer sequences used for real-time PCR.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

    doi: 10.1155/2013/475386

    Figure Lengend Snippet: The primer sequences used for real-time PCR.

    Article Snippet: Anti-PPAR γ , anti-aP2, anti-adiponectin, anti-phospho LKB1, anti-phospho AMPK α anti-AMPK α , and anti-ACC antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques:

    Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Rubi Fructus ( Rubus coreanus ) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

    doi: 10.1155/2013/475386

    Figure Lengend Snippet: Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μ g/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where P < 0.05 was considered a statistically significant difference from the differentiated control.

    Article Snippet: Anti-PPAR γ , anti-aP2, anti-adiponectin, anti-phospho LKB1, anti-phospho AMPK α anti-AMPK α , and anti-ACC antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .

    Journal: bioRxiv

    Article Title: Coordination Between Embryo Growth and Trophoblast Migration Upon Implantation Delineates Mouse Embryogenesis

    doi: 10.1101/2022.06.13.495767

    Figure Lengend Snippet: (A) Left, representative immunofluorescence image of the E4.75 uterus cross-section, simultaneously stained for Fibronectin (FN1, white), GATA4 (green), and nuclei (DNA, blue). Right, 4x zoom into the interface between trophectoderm (TE), uterine epithelium (E), and stroma (S) (outlined). Red arrowheads mark uterine ECM. (B) Representative immunofluorescence image of the E5.25 uterus cross-section, simultaneously stained for pan-Laminin (panLAM, white), GATA4 (green), and nuclei (DNA, blue). Measurements of E5.25 in utero embryo dimension across n = 12. (C) Schematic illustration of peri-implantation embryo culture. Embryos are recovered at E3.5, treated by Tyrode’s solution to remove Zona pellucida (ZP), and embedded into the crypts on the day of recovery (D0). ICM, red; TE, blue; IVC1 and ICV2 stand for “In Vitro Culture” medium 1 and 2, respectively. Inset, schematic illustration of the hydrogel composition. 8-arm Poly(ethylene glycol) (PEG, gray) molecules, crosslinked via metalloprotease-cleavable peptides (Peptide, green), and functionalized with RGDSPG peptide (Arg-Gly-Asp-Ser-Pro-Gly, ‘RGD’, red). (D) Representative immunofluorescence images of the embryos developed in utero until E4.5 and E5.25, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (E) Representative immunofluorescence images of the 3E-uterus embryos from day 2 and 3, simultaneously stained for OCT3/4 (magenta), GATA4 (green), and nuclei (DAPI, blue). (F) Scatterplot showing numbers of epiblast (EPI) cells (x-axis) vs numbers of visceral endoderm (VE) cells (y-axis) that cover epiblast in the embryos developed in utero until E5.5 (E3.5 – E5.5) and the embryos developed by 3E-uterus until day 3 (D1 – 3). n = 5 (E3.5), n = 21 (E4.5), n = 28 (E4.75), n = 20 (E5.0), n = 20 (E5.25), n = 21 (E5.5), data from ; n = 20, two replicates pooled (D1), n = 13 of 28, three replicates pooled (D2), n = 12 of 26, three replicates pooled (D3). X/Y scale, log 10. Bottom right, the scheme of the quantified embryo region, EPI (magenta), VE (green). (G) From left to right, boxplots showing egg cylinder’s length, diameter, and the length-to-diameter ratio between embryos developed in utero until E5.25 and 3E-uterus embryos from day 3 (D3). n = 14 and 12, respectively. Data points, shown as black dots, correspond to individual embryos, midline marks the median, boxes indicate interquartile range. P-values were calculated using Student’s t-test and the Mann-Whitney U test. (H) Cell number-based correspondence between in utero and 3E-uterus embryo development. (I) Left, representative immunofluorescence image of the E4.75 uterus cross-section from the pregnant F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 2 (D2). Yellow arrowheads mark differentiated trophoblast cells. (J) Left, representative immunofluorescence image of the E5.25 uterus cross-section from the F1 female mated with the H2B-GFP male. Simultaneous immunostaining for TFAP2C (yellow), GFP (green), and nuclei (DNA, blue). Right, immunostaining of the 3E-uterus embryo from day 3 (D3). Scale bars, 50 μm, 12.5 μm (4x zoom). White asterisks mark epiblast of the implanted embryos. See also Materials and Methods, .

    Article Snippet: Primary antibodies against GATA4 biotinylated (R&D systems, AF2606), SOX2 (Cell Signaling, 23064), TFAP2C (Cell Signaling, #2320), CDX2 (Biogenex Laboratories, MU392AUC), PARD6B (Santa Cruz Biotechnology, sc-67393), pan-Laminin (Novus Biologicals, NB300-144SS), Collagen IV (Millipore, AB756P), Fibronectin (Proteintech, 15613-1-AP), ITGB1 (Millipore, MAB1997), GFP (chromotek, gb2AF488) were diluted at 1:200.

    Techniques: Immunofluorescence, Staining, In Utero, Embryo Culture, In Vitro, MANN-WHITNEY, Immunostaining

    Journal: eLife

    Article Title: High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

    doi: 10.7554/eLife.59445

    Figure Lengend Snippet:

    Article Snippet: Antibody , anti-AP-2γ (Rabbit polyclonal) , Cell Signaling Technology , Cat# 2320, RRID: AB_2202287 , (1:800).

    Techniques: Recombinant, Multiplex Assay, Software

    KEY RESOURCES TABLE

    Journal: Cell stem cell

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    doi: 10.1016/j.stem.2018.12.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Primary antibodies are: K18 (1:800, R&D AF7619), K14 (1:800, BioLegend SIG-3476–100); AP-2γ (1:100, Cell Signaling 2320), p63 (1:100 Gene Tex GTX102425), ITGA6 (1:200, Millipore, MAB1378).

    Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

    KEY RESOURCES TABLE

    Journal: Cell stem cell

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    doi: 10.1016/j.stem.2018.12.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: AP-2γ antibody for IF , Cell Signaling , Cat #2320S RRID:AB_10695101.

    Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software

    KEY RESOURCES TABLE

    Journal: Cell stem cell

    Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

    doi: 10.1016/j.stem.2018.12.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: AP-2γ antibody for IF , Cell Signaling , Cat #2320S RRID:AB_10695101.

    Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Clone Assay, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software