rabbit anti app antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app antibody
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Rabbit Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti app antibody - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Amyloid precursor protein modulates β-catenin degradation"

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-4-29

    APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Figure Legend Snippet: APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.

    Techniques Used: Expressing, Over Expression, Infection, Plasmid Preparation, Western Blot

    APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).
    Figure Legend Snippet: APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).

    Techniques Used: Infection, Western Blot

    Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.
    Figure Legend Snippet: Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.

    Techniques Used: Infection, shRNA

    APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.
    Figure Legend Snippet: APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.

    Techniques Used: Expressing, Isolation, Infection, Staining, Fluorescence

    β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.
    Figure Legend Snippet: β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.

    Techniques Used: Knock-Out, Staining, Microscopy

    rabbit anti app antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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  • 94

    Structured Review

    Cell Signaling Technology Inc rabbit anti app antibody
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Rabbit Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti app antibody - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Amyloid precursor protein modulates β-catenin degradation"

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-4-29

    APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Figure Legend Snippet: APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.

    Techniques Used: Expressing, Over Expression, Infection, Plasmid Preparation, Western Blot

    APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).
    Figure Legend Snippet: APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).

    Techniques Used: Infection, Western Blot

    Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.
    Figure Legend Snippet: Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.

    Techniques Used: Infection, shRNA

    APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.
    Figure Legend Snippet: APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.

    Techniques Used: Expressing, Isolation, Infection, Staining, Fluorescence

    β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.
    Figure Legend Snippet: β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.

    Techniques Used: Knock-Out, Staining, Microscopy

    anti app antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti app antibody
    Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti app antibody - by Bioz Stars, 2023-02
    94/100 stars

    Images

    rabbit anti app  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc rabbit anti app
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti app - by Bioz Stars, 2023-02
    94/100 stars

    Images

    rabbit anti app  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti app
    Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in <t>young</t> <t>APP/PS1</t> transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti app - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Hippocampal CysLT1R overexpression or activation accelerates memory deficits, synaptic dysfunction, and amyloidogenesis in young APP/PS1 transgenic mice"

    Article Title: Hippocampal CysLT1R overexpression or activation accelerates memory deficits, synaptic dysfunction, and amyloidogenesis in young APP/PS1 transgenic mice

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-21-4518

    Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in young APP/PS1 transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in young APP/PS1 transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Western Blot

    Hippocampal CysLT1R overexpression or activation aggravates damages to synaptic structure and function in APP/PS1 transgenic mice. (A,C) The induction of hippocampal LTP was assessed after high-frequency stimulation and recorded for 60 min post-induction, and (B,D) mean values of fEPSPs were calculated during 55–60 min following the induction of LTP. (E) The synaptic density in the hippocampus was determined by electron microscopy. The red arrows indicate postsynaptic density. Scale bar =1 µm. (F,G) statistical analysis of synaptic density calculated as the number of synapses per 25 µm2. (H,J) Representative images of Golgi-Cox staining of dendritic spines in the hippocampus. Scale bar =10 µm. (I,K) Statistical analysis of the average number of dendritic spines. (L,N) The protein levels of PSD-95 were detected in hippocampus using western blot and (M,O) quantified as ratio (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05, **P<0.01, ***P<0.001 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; LTP, long-term potentiation; fEPSPs, field excitatory postsynaptic potentials; WT, wild type; EGFP, enhanced green fluorescent protein; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R overexpression or activation aggravates damages to synaptic structure and function in APP/PS1 transgenic mice. (A,C) The induction of hippocampal LTP was assessed after high-frequency stimulation and recorded for 60 min post-induction, and (B,D) mean values of fEPSPs were calculated during 55–60 min following the induction of LTP. (E) The synaptic density in the hippocampus was determined by electron microscopy. The red arrows indicate postsynaptic density. Scale bar =1 µm. (F,G) statistical analysis of synaptic density calculated as the number of synapses per 25 µm2. (H,J) Representative images of Golgi-Cox staining of dendritic spines in the hippocampus. Scale bar =10 µm. (I,K) Statistical analysis of the average number of dendritic spines. (L,N) The protein levels of PSD-95 were detected in hippocampus using western blot and (M,O) quantified as ratio (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05, **P<0.01, ***P<0.001 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; LTP, long-term potentiation; fEPSPs, field excitatory postsynaptic potentials; WT, wild type; EGFP, enhanced green fluorescent protein; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Electron Microscopy, Staining, Western Blot

    Hippocampal CysLT1R Overexpression or activation exacerbates Aβ generation in young APP/PS1 transgenic mice. (A,B) The TBS-soluble forms of Aβ1-40 in hippocampus were assessed by ELISA. (C,D) Representative western blot of APP, BACE, and PS1 in hippocampus. Quantification of (E,F) APP, (G,I) BACE, (H,J) PS1 levels were expressed as ration (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; TBS, Tris-buffered saline; Aβ, amyloid β; ELISA, enzyme-linked immunosorbent assay; APP, amyloid precursor protein; BACE, β-secretase; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R Overexpression or activation exacerbates Aβ generation in young APP/PS1 transgenic mice. (A,B) The TBS-soluble forms of Aβ1-40 in hippocampus were assessed by ELISA. (C,D) Representative western blot of APP, BACE, and PS1 in hippocampus. Quantification of (E,F) APP, (G,I) BACE, (H,J) PS1 levels were expressed as ration (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; TBS, Tris-buffered saline; Aβ, amyloid β; ELISA, enzyme-linked immunosorbent assay; APP, amyloid precursor protein; BACE, β-secretase; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Enzyme-linked Immunosorbent Assay, Western Blot

    rabbit anti app  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit anti app
    Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in <t>young</t> <t>APP/PS1</t> transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Images

    1) Product Images from "Hippocampal CysLT1R overexpression or activation accelerates memory deficits, synaptic dysfunction, and amyloidogenesis in young APP/PS1 transgenic mice"

    Article Title: Hippocampal CysLT1R overexpression or activation accelerates memory deficits, synaptic dysfunction, and amyloidogenesis in young APP/PS1 transgenic mice

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-21-4518

    Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in young APP/PS1 transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R overexpression or activation accelerates cognitive impairment in young APP/PS1 transgenic mice. (A) Representative hippocampus area with LV-CysLT1R-EGFP. (B) The hippocampal CysLT1R protein, and (C) quantified as ration (in%) of WT + EGFP group. (D,E) The mean escape latency to the visible platform and the hidden platform, (F) the percentage of time spent in the target quadrant, and (G) the numbers of platform location crossings during the probe trial in the MWM task, and (H) the number of correct choices, (I) the latency to enter the correct compartment in the Y-maze test, and (J) the discrimination index in the NOR test in young APP/PS1 mice treated with LV-CysLT1R. (K) The percentage of time spent in the target quadrant, and (L) the numbers of platform location crossings during the probe trial in the MWM task in young APP/PS1 mice treated with YM-17690. Values shown are expressed as mean ± SEM; n=4/group for Western blot, n=9–10/group for behavior tests. *P<0.05, **P<0.01 vs. 6-month-old APP/PS1 + EGFP group or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; WT, wild type; EGFP, enhanced green fluorescent protein; NOR, novel object recognition; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Western Blot

    Hippocampal CysLT1R overexpression or activation aggravates damages to synaptic structure and function in APP/PS1 transgenic mice. (A,C) The induction of hippocampal LTP was assessed after high-frequency stimulation and recorded for 60 min post-induction, and (B,D) mean values of fEPSPs were calculated during 55–60 min following the induction of LTP. (E) The synaptic density in the hippocampus was determined by electron microscopy. The red arrows indicate postsynaptic density. Scale bar =1 µm. (F,G) statistical analysis of synaptic density calculated as the number of synapses per 25 µm2. (H,J) Representative images of Golgi-Cox staining of dendritic spines in the hippocampus. Scale bar =10 µm. (I,K) Statistical analysis of the average number of dendritic spines. (L,N) The protein levels of PSD-95 were detected in hippocampus using western blot and (M,O) quantified as ratio (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05, **P<0.01, ***P<0.001 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; LTP, long-term potentiation; fEPSPs, field excitatory postsynaptic potentials; WT, wild type; EGFP, enhanced green fluorescent protein; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R overexpression or activation aggravates damages to synaptic structure and function in APP/PS1 transgenic mice. (A,C) The induction of hippocampal LTP was assessed after high-frequency stimulation and recorded for 60 min post-induction, and (B,D) mean values of fEPSPs were calculated during 55–60 min following the induction of LTP. (E) The synaptic density in the hippocampus was determined by electron microscopy. The red arrows indicate postsynaptic density. Scale bar =1 µm. (F,G) statistical analysis of synaptic density calculated as the number of synapses per 25 µm2. (H,J) Representative images of Golgi-Cox staining of dendritic spines in the hippocampus. Scale bar =10 µm. (I,K) Statistical analysis of the average number of dendritic spines. (L,N) The protein levels of PSD-95 were detected in hippocampus using western blot and (M,O) quantified as ratio (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05, **P<0.01, ***P<0.001 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; LTP, long-term potentiation; fEPSPs, field excitatory postsynaptic potentials; WT, wild type; EGFP, enhanced green fluorescent protein; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Electron Microscopy, Staining, Western Blot

    Hippocampal CysLT1R Overexpression or activation exacerbates Aβ generation in young APP/PS1 transgenic mice. (A,B) The TBS-soluble forms of Aβ1-40 in hippocampus were assessed by ELISA. (C,D) Representative western blot of APP, BACE, and PS1 in hippocampus. Quantification of (E,F) APP, (G,I) BACE, (H,J) PS1 levels were expressed as ration (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; TBS, Tris-buffered saline; Aβ, amyloid β; ELISA, enzyme-linked immunosorbent assay; APP, amyloid precursor protein; BACE, β-secretase; SEM, standard error of the mean.
    Figure Legend Snippet: Hippocampal CysLT1R Overexpression or activation exacerbates Aβ generation in young APP/PS1 transgenic mice. (A,B) The TBS-soluble forms of Aβ1-40 in hippocampus were assessed by ELISA. (C,D) Representative western blot of APP, BACE, and PS1 in hippocampus. Quantification of (E,F) APP, (G,I) BACE, (H,J) PS1 levels were expressed as ration (in%) of WT + EGFP/WT + Vehicle group. Values shown are expressed as mean ± SEM; n=4–6/group; *P<0.05 vs. 6-month-old APP/PS1 + EGFP or 6-month-old APP/PS1 + Vehicle group. CysLT1R, cysteinyl leukotriene receptor 1; TBS, Tris-buffered saline; Aβ, amyloid β; ELISA, enzyme-linked immunosorbent assay; APP, amyloid precursor protein; BACE, β-secretase; SEM, standard error of the mean.

    Techniques Used: Over Expression, Activation Assay, Transgenic Assay, Enzyme-linked Immunosorbent Assay, Western Blot

    rabbit anti app antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app antibody
    Effects of spinosin on the expression of <t>APP</t> <t>(A),</t> <t>BACE1</t> (B) and ADAM10 (C) proteins. Protein levels were determined by western blot. All western blot data were normalized by β-actin. Values are the mean ± SEM from experiments performed in triplicate. Significance was determined by Tukey’s multiple comparisons test. * p <0.05, ** p <0.01, *** p <0.001 versus untreated N2a/APP695 cells; # p <0.05, ## p <0.01, ### p <0.001 versus untreated N2a/WT cells.
    Rabbit Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Spinosin Inhibits Aβ 1-42 Production and Aggregation via Activating Nrf2/HO-1 Pathway"

    Article Title: Spinosin Inhibits Aβ 1-42 Production and Aggregation via Activating Nrf2/HO-1 Pathway

    Journal: Biomolecules & Therapeutics

    doi: 10.4062/biomolther.2019.123

    Effects of spinosin on the expression of APP (A), BACE1 (B) and ADAM10 (C) proteins. Protein levels were determined by western blot. All western blot data were normalized by β-actin. Values are the mean ± SEM from experiments performed in triplicate. Significance was determined by Tukey’s multiple comparisons test. * p <0.05, ** p <0.01, *** p <0.001 versus untreated N2a/APP695 cells; # p <0.05, ## p <0.01, ### p <0.001 versus untreated N2a/WT cells.
    Figure Legend Snippet: Effects of spinosin on the expression of APP (A), BACE1 (B) and ADAM10 (C) proteins. Protein levels were determined by western blot. All western blot data were normalized by β-actin. Values are the mean ± SEM from experiments performed in triplicate. Significance was determined by Tukey’s multiple comparisons test. * p <0.05, ** p <0.01, *** p <0.001 versus untreated N2a/APP695 cells; # p <0.05, ## p <0.01, ### p <0.001 versus untreated N2a/WT cells.

    Techniques Used: Expressing, Western Blot

    Nrf2 inhibitor treatment reversed the role of spinosin in N2a cells. The expressions of APP (A, B), BACE1 (C, D), ADAM10 (E, F) and HO-1 (G, H) were detected by western blot. And they were normalized by β-actin. Values are the mean ± SEM from experiments performed in triplicate. * p <0.05, ** p <0.01, *** p <0.001 versus untreated N2a/APP695 cells; # p <0.05, ## p <0.01, ### p <0.001 versus untreated N2a/WT cells.
    Figure Legend Snippet: Nrf2 inhibitor treatment reversed the role of spinosin in N2a cells. The expressions of APP (A, B), BACE1 (C, D), ADAM10 (E, F) and HO-1 (G, H) were detected by western blot. And they were normalized by β-actin. Values are the mean ± SEM from experiments performed in triplicate. * p <0.05, ** p <0.01, *** p <0.001 versus untreated N2a/APP695 cells; # p <0.05, ## p <0.01, ### p <0.001 versus untreated N2a/WT cells.

    Techniques Used: Western Blot

    app rabbit antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc app rabbit antibody
    App Rabbit Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti app antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app antibody
    Rabbit Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    rabbit anti app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
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    rabbit anti app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app
    Immunoblot analysis (A) and protein levels of <t>mature</t> <t>BACE1</t> (B) and <t>APP</t> (C) and quantification of Aβ 1–42 by ELISA (D) in WT and PKR -/- mice treated with saline or LPS. LPS induces BACE1 maturation and Aβ production without altering APP levels in WT mice. PKR inhibition prevents BACE1 maturation and Aβ production after LPS treatment. Transcriptional activity on BACE1 in hippocampus assessed with qRT-PCR and normalized to GAPDH mRNA levels (E). Immunoblots (A) have been cropped in this figure. Full length version of BACE1 and APP blots are available in the . WT [n = 4], PKR -/- [n = 4], **p < 0.01.
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    1) Product Images from "Neuroinflammation and Aβ Accumulation Linked To Systemic Inflammation Are Decreased By Genetic PKR Down-Regulation"

    Article Title: Neuroinflammation and Aβ Accumulation Linked To Systemic Inflammation Are Decreased By Genetic PKR Down-Regulation

    Journal: Scientific Reports

    doi: 10.1038/srep08489

    Immunoblot analysis (A) and protein levels of mature BACE1 (B) and APP (C) and quantification of Aβ 1–42 by ELISA (D) in WT and PKR -/- mice treated with saline or LPS. LPS induces BACE1 maturation and Aβ production without altering APP levels in WT mice. PKR inhibition prevents BACE1 maturation and Aβ production after LPS treatment. Transcriptional activity on BACE1 in hippocampus assessed with qRT-PCR and normalized to GAPDH mRNA levels (E). Immunoblots (A) have been cropped in this figure. Full length version of BACE1 and APP blots are available in the . WT [n = 4], PKR -/- [n = 4], **p < 0.01.
    Figure Legend Snippet: Immunoblot analysis (A) and protein levels of mature BACE1 (B) and APP (C) and quantification of Aβ 1–42 by ELISA (D) in WT and PKR -/- mice treated with saline or LPS. LPS induces BACE1 maturation and Aβ production without altering APP levels in WT mice. PKR inhibition prevents BACE1 maturation and Aβ production after LPS treatment. Transcriptional activity on BACE1 in hippocampus assessed with qRT-PCR and normalized to GAPDH mRNA levels (E). Immunoblots (A) have been cropped in this figure. Full length version of BACE1 and APP blots are available in the . WT [n = 4], PKR -/- [n = 4], **p < 0.01.

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Inhibition, Activity Assay, Quantitative RT-PCR

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    Cell Signaling Technology Inc rabbit anti app antibody
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Rabbit Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    Cell Signaling Technology Inc anti app antibody
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Anti App Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti app antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti app antibody - by Bioz Stars, 2023-02
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    94
    Cell Signaling Technology Inc rabbit anti app
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    Rabbit Anti App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti app/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti app - by Bioz Stars, 2023-02
    94/100 stars
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    94
    Cell Signaling Technology Inc app rabbit antibody
    <t>APP</t> expression induced degradation <t>of</t> <t>β-catenin</t> . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.
    App Rabbit Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/app rabbit antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    app rabbit antibody - by Bioz Stars, 2023-02
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    Image Search Results


    APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.

    Journal: Journal of Neuroinflammation

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    doi: 10.1186/1742-2094-4-29

    Figure Lengend Snippet: APP expression induced degradation of β-catenin . A. Overexpression of APP reduces β-catenin levels. Human APP 695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control ( p < 0.05, t -Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.

    Article Snippet: The following antibodies were used: rabbit anti-β-catenin and mouse anti-γ-tubulin (both from Sigma); anti-phosphorylated β-catenin antibodies, S33/37/T41 and S45 (both from Cell Signaling); rabbit anti-APP antibody (369, a gift from Dr. Sam Gandy).

    Techniques: Expressing, Over Expression, Infection, Plasmid Preparation, Western Blot

    APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).

    Journal: Journal of Neuroinflammation

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    doi: 10.1186/1742-2094-4-29

    Figure Lengend Snippet: APP facilitates β-catenin degradation by increasing β-catenin phosphorylation at S33/37/T41 residues . A. Primary neurons infected with APP contained less steady-state β-catenin and higher levels of phosphorylated S33/37/T41 than did neurons infected with control LacZ (2 IU virus per cell). Levels of phosphorylated S45 did not appear to change. B. Quantitative analyses of western blots from 3 independent experiments. APP significantly increased β-catenin phosphorylation at S33/37/T41 residues (p = 0.02, t -Test), but not at S45 residue (p = 0.19, t -Test).

    Article Snippet: The following antibodies were used: rabbit anti-β-catenin and mouse anti-γ-tubulin (both from Sigma); anti-phosphorylated β-catenin antibodies, S33/37/T41 and S45 (both from Cell Signaling); rabbit anti-APP antibody (369, a gift from Dr. Sam Gandy).

    Techniques: Infection, Western Blot

    Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.

    Journal: Journal of Neuroinflammation

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    doi: 10.1186/1742-2094-4-29

    Figure Lengend Snippet: Suppression of endogenous APP results in increased total β-catenin and decreased S33/37/T41-phosphorylated β-catenin . Primary neurons were infected with APPshRNA (APP1996) or missense shRNA viruses (0.5 IU/cell) for 14 h before harvest and protein analyses. The mean optical densities of 3 independent experiments for each protein analyzed are presented in the Y-axis in the bar graphs. The statistical significance of difference is also presented in each bar graph ( p values from t -Test) for each protein analyzed (labelled on the Y-axis). Representative blots are shown on the right. Loading controls were γ-tubulin reprobed from the same blots.

    Article Snippet: The following antibodies were used: rabbit anti-β-catenin and mouse anti-γ-tubulin (both from Sigma); anti-phosphorylated β-catenin antibodies, S33/37/T41 and S45 (both from Cell Signaling); rabbit anti-APP antibody (369, a gift from Dr. Sam Gandy).

    Techniques: Infection, shRNA

    APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.

    Journal: Journal of Neuroinflammation

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    doi: 10.1186/1742-2094-4-29

    Figure Lengend Snippet: APP expression downregulates β-catenin levels in membrane and cytosolic fractions but not in the nuclear fraction . A. β-Catenin levels from membrane and cytosolic fractions isolated from primary neurons. Wild type or FAD APPs were overexpressed in primary neurons via HSV-mediated gene transfer using LacZ as the control. 10 μg protein per sample was analyzed in each fraction. B. Selected z-series of single cells. Scale bar, 2 μm. C. Composite images from z-series. Scale bar, 5 μm. In both B and C, primary neurons were infected with APP or the control LacZ viruses at 2 IU/cell for 14 h. Cells were then fixed and stained with rabbit anti-β-catenin and Alexa fluor 488 goat anti-rabbit. Images were collected at the same settings for fluorescence intensity comparison.

    Article Snippet: The following antibodies were used: rabbit anti-β-catenin and mouse anti-γ-tubulin (both from Sigma); anti-phosphorylated β-catenin antibodies, S33/37/T41 and S45 (both from Cell Signaling); rabbit anti-APP antibody (369, a gift from Dr. Sam Gandy).

    Techniques: Expressing, Isolation, Infection, Staining, Fluorescence

    β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.

    Journal: Journal of Neuroinflammation

    Article Title: Amyloid precursor protein modulates β-catenin degradation

    doi: 10.1186/1742-2094-4-29

    Figure Lengend Snippet: β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls . Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.

    Article Snippet: The following antibodies were used: rabbit anti-β-catenin and mouse anti-γ-tubulin (both from Sigma); anti-phosphorylated β-catenin antibodies, S33/37/T41 and S45 (both from Cell Signaling); rabbit anti-APP antibody (369, a gift from Dr. Sam Gandy).

    Techniques: Knock-Out, Staining, Microscopy