Journal: PLoS ONE

Article Title: Molecular Basis for Vulnerability to Mitochondrial and Oxidative Stress in a Neuroendocrine CRI-G1 Cell Line

doi: 10.1371/journal.pone.0014485

Figure Lengend Snippet: A . At baseline, Aldh2 protein level (apparent MW, 51 kDa) in the whole cell lysate is significantly lower in CRI-G1-RS than in CRI-G1-RR cells. Statistical significance was determined by two-tailed Student's t-test; scatter plot of all data points is shown. B. Aldh2 expression was not affected by 24 h treatment with 5 µM SF or 10 µM tBHQ (two activators of Nrf2 pathway) in either cell type; regardless of the treatment, Aldh2 expression level was significantly lower in CRI-G1-RS than in CRI-G1-RR cells. Statistical significance was determined by two-way ANOVA; data are plotted as mean ± S.E.M. (n = 5). ***, p<0.001.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-PARK7/DJ-1, 1 µg/mL (GenWay Biotech); mouse monoclonal anti-Keap1, 1 µg/mL (ProteinTech Group); mouse monoclonal anti-COXIV 20E8, 0.1 µg/mL (Abcam); mouse monoclonal anti-cytochrome c, 0.5 µg/mL (BD Pharmingen); rabbit polyclonal anti-Tom20 FL-145, 0.07 µg/mL, and rabbit polyclonal Nrf2 H300, 0.5 µg/mL (Santa Cruz Biotechnology); rabbit polyclonal anti-Aldh2, 1∶10000 (gift of Dr. Henry Weiner, Purdue University); rabbit polyclonal anti-Akt and anti-phospho-Akt, 1∶1000 (Cell Signaling); mouse monoclonal anti-tubulin clone DM1A (ascites fluid), 1 µg/mL, and rabbit polyclonal anti-lamin A C-terminal, 1 µg/mL (Sigma).

Techniques: Two Tailed Test, Expressing

Journal: ecancermedicalscience

Article Title: Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients

doi: 10.3332/ecancer.2014.404

Figure Lengend Snippet: CK 8/18 cytoplasmic staining (loss of membranous pattern) in invasive duct carcinoma (immunoperoxidase ×400).

Article Snippet: CK 8/18 is important for cellular processes, such as cell signalling [ ], mitosis [ ], cell cycle progression [ ], and protection from apoptosis [ , ].

Techniques: Staining

Journal: ecancermedicalscience

Article Title: Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients

doi: 10.3332/ecancer.2014.404

Figure Lengend Snippet: CK 8/18 with preserved membranous pattern in invasive duct carcinoma (immunoperoxidase ×400).

Article Snippet: CK 8/18 is important for cellular processes, such as cell signalling [ ], mitosis [ ], cell cycle progression [ ], and protection from apoptosis [ , ].

Techniques:

Journal: ecancermedicalscience

Article Title: Relationship of CK8/18 expression pattern to breast cancer immunohistochemical subtyping in Egyptian patients

doi: 10.3332/ecancer.2014.404

Figure Lengend Snippet: Association between CK 8/18 pattern of expression and the clinico-pathological parameters.

Article Snippet: CK 8/18 is important for cellular processes, such as cell signalling [ ], mitosis [ ], cell cycle progression [ ], and protection from apoptosis [ , ].

Techniques: Expressing, Staining

Journal: Cell Reports Medicine

Article Title: Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer

doi: 10.1016/j.xcrm.2022.100604

Figure Lengend Snippet:

Article Snippet: anti-human-Cytokeratin 8/18 (C51) , Cell Signaling Technologies , Cat# 4546; RRID: AB_2134843.

Techniques: Recombinant, Blocking Assay, Electron Microscopy, Labeling, Mass Cytometry, Software

Journal: iScience

Article Title: EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer

doi: 10.1016/j.isci.2022.104193

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Keratin 8/18 , Cell Signaling Technology , Cat# 4546; RRID: AB_2134843.

Techniques: Plasmid Preparation, shRNA, Microarray, Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Glutathione Assay, Enzyme-linked Immunosorbent Assay, Expressing, Isolation, Software, Transfection