Journal: Biomolecules & Therapeutics

Article Title: Effects of Dextran Sulfate Sodium-Induced Ulcerative Colitis on the Disposition of Tofacitinib in Rats

doi: 10.4062/biomolther.2022.049

Figure Lengend Snippet: Immunoblot analyses of (A) CYP450 isozymes and (B) PXR, CAR and P-gp of hepatic and intestinal microsomes in control (CON) and dextran sulfate sodium-induced ulcerative colitis (DSS) rats. β-actin was determined as a loading control. This experiment was completed three times. Band density was measured using ImageJ1.45s software (NIH). CAR, constitutive androstane receptor; PXR, pregnane X receptor; P-gp, P-glycoprotein.

Article Snippet: Primary antibodies against pregnane X receptor (PXR), constitutive androstane receptor (CAR), P-gp and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Western Blot, Software

Journal:

Article Title: Effective High-Capacity Gutless Adenoviral Vectors Mediate Transgene Expression in Human Glioma Cells

doi: 10.1016/j.ymthe.2006.05.006

Figure Lengend Snippet: Expression of coxsackie and adenovirus receptor (CAR) and integrin αvβ3 (INT) in human and murine glioma cells. (A) CAR and (B) integrin αvβ3 expression was determined by Western blot analysis in human glioma cell lines (U251, U87), cultures from human glioma biopsies (IN859, IN 2045), rat (CNS-1), and mouse (GL26) glioma cells. CHO and HeLa cells were used as negative and positive controls, respectively. β-Actin was used as loading control. The tops show Western blots for CAR (A), INT (B), and β-actin from glioma, CHO, and HeLa cell lysates. The bottom panels show the integrated density values of CAR or INT in relation to β-actin expression for all cells analyzed.

Article Snippet: Primary anti-CAR (Upstate Cell Signaling Solutions), anti-integrin α v β 3 (Chemicon International), or anti-actin antibodies (Sigma) were detected with HRP-conjugated antibodies and developed with an ECL kit (Amersham Biosciences).

Techniques: Expressing, Western Blot