epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and <t>EpCAM</t> <t>antibodies,</t> detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Deep Visual Proteomics defines single-cell identity and heterogeneity"

    Article Title: Deep Visual Proteomics defines single-cell identity and heterogeneity

    Journal: Nature Biotechnology

    doi: 10.1038/s41587-022-01302-5

    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).
    Figure Legend Snippet: a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).

    Techniques Used: Microscopy, Immunofluorescence, Staining, Marker, Derivative Assay

    epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and <t>EpCAM</t> <t>antibodies,</t> detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Deep Visual Proteomics defines single-cell identity and heterogeneity"

    Article Title: Deep Visual Proteomics defines single-cell identity and heterogeneity

    Journal: Nature Biotechnology

    doi: 10.1038/s41587-022-01302-5

    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).
    Figure Legend Snippet: a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).

    Techniques Used: Microscopy, Immunofluorescence, Staining, Marker, Derivative Assay

    anti epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti epcam
    a Dot plot showing <t>relative</t> <t>FAP</t> expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an <t>EpCAM</t> staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).
    Anti Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Fibroblast activation protein identifies Consensus Molecular Subtype 4 in colorectal cancer and allows its detection by 68 Ga-FAPI-PET imaging"

    Article Title: Fibroblast activation protein identifies Consensus Molecular Subtype 4 in colorectal cancer and allows its detection by 68 Ga-FAPI-PET imaging

    Journal: British Journal of Cancer

    doi: 10.1038/s41416-022-01748-z

    a Dot plot showing relative FAP expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an EpCAM staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).
    Figure Legend Snippet: a Dot plot showing relative FAP expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an EpCAM staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).

    Techniques Used: Expressing, Derivative Assay, Immunohistochemistry, Staining

    a Immunohistochemistry for FAP on peritoneal metastases tissue that was resected one year prior to FAPI-PET imaging. b Immunohistochemistry of EpCAM-positive tumour cells in the peritoneum show a strong ZEB1 and HTR2B staining. c FAPI-PET image shows tracer accumulation in pelvic peritoneal depositions and along the abdominal peritoneal lining (white arrows). See also Supplementary Fig. . d FAPI-PET image (same coronal section, same scaling)) after 2 months of chemotherapy shows a reduction of FAPI tracer uptake in the pelvic lesion and the serosal lesion and a complete loss of diffuse FAPI tracer uptake along the peritoneum.
    Figure Legend Snippet: a Immunohistochemistry for FAP on peritoneal metastases tissue that was resected one year prior to FAPI-PET imaging. b Immunohistochemistry of EpCAM-positive tumour cells in the peritoneum show a strong ZEB1 and HTR2B staining. c FAPI-PET image shows tracer accumulation in pelvic peritoneal depositions and along the abdominal peritoneal lining (white arrows). See also Supplementary Fig. . d FAPI-PET image (same coronal section, same scaling)) after 2 months of chemotherapy shows a reduction of FAPI tracer uptake in the pelvic lesion and the serosal lesion and a complete loss of diffuse FAPI tracer uptake along the peritoneum.

    Techniques Used: Immunohistochemistry, Imaging, Staining

    anti epcam antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti epcam antibody
    Anti Epcam Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epcam/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    Anti-tumor capability of epithelial cell adhesion molecule-chimeric antigen receptor-T <t>(EpCAM-CAR-T)</t> at the in vitro level. A) The cytotoxicity of EpCAM-CAR-T cells against SKOV3 cells was analyzed by Real Time Cell Analysis assay. B) Levels of Interferon-γ (IFNγ) released by EpCAM-CAR-T cells analyzed by ELISA after incubated with ovarian cancer (OC) cells for 24 hr. Untransduced T cells were used as NC-T. Error bars denote the s.e.m., and the results were compared with two-way ANOVA test. *** P <0.001.
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epcam/product/Cell Signaling Technology Inc
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    Images

    1) Product Images from "Chimeric Antigen receptor-T (CAR-T) cells targeting Epithelial cell adhesion molecule (EpCAM) can inhibit tumor growth in ovarian cancer mouse model"

    Article Title: Chimeric Antigen receptor-T (CAR-T) cells targeting Epithelial cell adhesion molecule (EpCAM) can inhibit tumor growth in ovarian cancer mouse model

    Journal: The Journal of Veterinary Medical Science

    doi: 10.1292/jvms.20-0455

    Anti-tumor capability of epithelial cell adhesion molecule-chimeric antigen receptor-T (EpCAM-CAR-T) at the in vitro level. A) The cytotoxicity of EpCAM-CAR-T cells against SKOV3 cells was analyzed by Real Time Cell Analysis assay. B) Levels of Interferon-γ (IFNγ) released by EpCAM-CAR-T cells analyzed by ELISA after incubated with ovarian cancer (OC) cells for 24 hr. Untransduced T cells were used as NC-T. Error bars denote the s.e.m., and the results were compared with two-way ANOVA test. *** P <0.001.
    Figure Legend Snippet: Anti-tumor capability of epithelial cell adhesion molecule-chimeric antigen receptor-T (EpCAM-CAR-T) at the in vitro level. A) The cytotoxicity of EpCAM-CAR-T cells against SKOV3 cells was analyzed by Real Time Cell Analysis assay. B) Levels of Interferon-γ (IFNγ) released by EpCAM-CAR-T cells analyzed by ELISA after incubated with ovarian cancer (OC) cells for 24 hr. Untransduced T cells were used as NC-T. Error bars denote the s.e.m., and the results were compared with two-way ANOVA test. *** P <0.001.

    Techniques Used: In Vitro, Enzyme-linked Immunosorbent Assay, Incubation

    Anti-tumor capability of epithelial cell adhesion molecule-chimeric antigen receptor-T (EpCAM-CAR-T) at the level of in vivo . A) Schema of the experimental events and nodes. In this process, model mice were injected with 1 × 10 6 ovarian cancer (OC) cells SKOV3. Seven days after injection, 12 mice were randomly divided into 3 groups: EpCAM-CAR-T cells treated group, control-T cells treated group and Phosphate buffer saline (PBS) of the same volume treated group. B) The tumor size variation with EpCAM-CAR-T cells, control T cells or PBS injection among the 41 days. Untransduced T cells were used as NC-T. Error bars denote the s.e.m., and the results were compared with two-way ANOVA test. *** P <0.001.
    Figure Legend Snippet: Anti-tumor capability of epithelial cell adhesion molecule-chimeric antigen receptor-T (EpCAM-CAR-T) at the level of in vivo . A) Schema of the experimental events and nodes. In this process, model mice were injected with 1 × 10 6 ovarian cancer (OC) cells SKOV3. Seven days after injection, 12 mice were randomly divided into 3 groups: EpCAM-CAR-T cells treated group, control-T cells treated group and Phosphate buffer saline (PBS) of the same volume treated group. B) The tumor size variation with EpCAM-CAR-T cells, control T cells or PBS injection among the 41 days. Untransduced T cells were used as NC-T. Error bars denote the s.e.m., and the results were compared with two-way ANOVA test. *** P <0.001.

    Techniques Used: In Vivo, Injection

    epcam  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam
    Western blot analysis of tumor-associated markers. ( A ) Carbonic anhydrase <t>9</t> <t>(CA9),</t> CD70, and epithelial cell adhesion molecule <t>(EpCAM);</t> ( B ) CD147) on exosomes from ccRCC (786-O, Caki2, Caki1, and RCC53) and control (MCF7) cell lines.
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Characterization of CD147, CA9, and CD70 as Tumor-Specific Markers on Extracellular Vesicles in Clear Cell Renal Cell Carcinoma"

    Article Title: Characterization of CD147, CA9, and CD70 as Tumor-Specific Markers on Extracellular Vesicles in Clear Cell Renal Cell Carcinoma

    Journal: Diagnostics

    doi: 10.3390/diagnostics10121034

    Western blot analysis of tumor-associated markers. ( A ) Carbonic anhydrase 9 (CA9), CD70, and epithelial cell adhesion molecule (EpCAM); ( B ) CD147) on exosomes from ccRCC (786-O, Caki2, Caki1, and RCC53) and control (MCF7) cell lines.
    Figure Legend Snippet: Western blot analysis of tumor-associated markers. ( A ) Carbonic anhydrase 9 (CA9), CD70, and epithelial cell adhesion molecule (EpCAM); ( B ) CD147) on exosomes from ccRCC (786-O, Caki2, Caki1, and RCC53) and control (MCF7) cell lines.

    Techniques Used: Western Blot

    Western blot analysis of tumor-associated markers (CD147, CA9, CD70, EpCAM) in samples derived from renal tumors and normal tissues (NTB1175 and NTB1302). L = lysate, H = homogenate, F0–F3 = fractions 0–3, MW = molecular weight, kDa.
    Figure Legend Snippet: Western blot analysis of tumor-associated markers (CD147, CA9, CD70, EpCAM) in samples derived from renal tumors and normal tissues (NTB1175 and NTB1302). L = lysate, H = homogenate, F0–F3 = fractions 0–3, MW = molecular weight, kDa.

    Techniques Used: Western Blot, Derivative Assay, Molecular Weight

    The detection of tumor-associated markers (CD147,  CA9,  CD70, and  EpCAM)  by Western blot in cells and tumor-derived exosomes from patient samples. The number of positive samples is related to the total sample number analyzed.
    Figure Legend Snippet: The detection of tumor-associated markers (CD147, CA9, CD70, and EpCAM) by Western blot in cells and tumor-derived exosomes from patient samples. The number of positive samples is related to the total sample number analyzed.

    Techniques Used: Western Blot

    Histopathological characteristics and Western blot results on tumor tissue samples. Semiquantitative evaluation: “-” = no expression; “+” = low expression; “++” = moderate expression; “+++” = high expression; n.e. = not examined. TNM = Tumor Node Metastasis Classification of Malignant Tumors.
    Figure Legend Snippet: Histopathological characteristics and Western blot results on tumor tissue samples. Semiquantitative evaluation: “-” = no expression; “+” = low expression; “++” = moderate expression; “+++” = high expression; n.e. = not examined. TNM = Tumor Node Metastasis Classification of Malignant Tumors.

    Techniques Used: Western Blot, Expressing

    Representative images of the immunohistochemical staining of tumor markers (CA9, CD147, CD70, and EpCAM) on formalin-fixed paraffin-embedded (FFPE) sections of tumor and normal primary tissue samples from patients with clear cell renal carcinoma (ccRCC). Yellow arrow indicates an infiltrating immune cell. Scale bar = 100 µm.
    Figure Legend Snippet: Representative images of the immunohistochemical staining of tumor markers (CA9, CD147, CD70, and EpCAM) on formalin-fixed paraffin-embedded (FFPE) sections of tumor and normal primary tissue samples from patients with clear cell renal carcinoma (ccRCC). Yellow arrow indicates an infiltrating immune cell. Scale bar = 100 µm.

    Techniques Used: Immunohistochemical staining, Staining, Formalin-fixed Paraffin-Embedded

    epcam d9s3p  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc epcam d9s3p
    Epcam D9s3p, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti epcam
    Anti Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti epcam/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    Cell Signaling Technology Inc anti epcam
    Anti Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti epcam/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc epcam
    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and <t>EpCAM</t> <t>antibodies,</t> detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).
    Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epcam/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    Cell Signaling Technology Inc anti epcam
    a Dot plot showing <t>relative</t> <t>FAP</t> expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an <t>EpCAM</t> staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).
    Anti Epcam, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti epcam/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti epcam antibody
    a Dot plot showing <t>relative</t> <t>FAP</t> expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an <t>EpCAM</t> staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).
    Anti Epcam Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti epcam antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti epcam antibody - by Bioz Stars, 2023-02
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    Cell Signaling Technology Inc epcam d9s3p
    a Dot plot showing <t>relative</t> <t>FAP</t> expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an <t>EpCAM</t> staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).
    Epcam D9s3p, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epcam d9s3p/product/Cell Signaling Technology Inc
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    Image Search Results


    a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).

    Journal: Nature Biotechnology

    Article Title: Deep Visual Proteomics defines single-cell identity and heterogeneity

    doi: 10.1038/s41587-022-01302-5

    Figure Lengend Snippet: a , AI-driven nucleus and cytoplasm segmentation of normal-appearing and cancer cells and tissue using BIAS. b , We benchmarked the accuracy of its segmentation approach using the F1 metric and compared results to three additional methods—M 1 is unet4nuclei , M 2 is CellProfiler and M is Cellpose —while OUR refers to nucleAIzer . Bars show mean F1 scores with s.e.m.; n = 10 independent images for melanoma tissue and (U2OS) cells, and n = 20 for salivary gland tissue. Visual representation of the segmentation results: green areas correspond to true positive, blue to false positive and red to false negative. c , BIAS serves as the interface between the scanning and an LMD microscope, allowing high-accuracy transfers of cell contours between the microscopes. Illustration of cutting offset with respect to the object of interest and optimal path finding. d , Practical illustration of the functions in the upper panel. e , Immunofluorescence staining of the human fallopian tube epithelium with FOXJ1 and EpCAM antibodies, detecting ciliated and epithelial cells, respectively. Left panel: Ciliated (FOXJ1-positive) and secretory (FOXJ1-negative) cells. Right panel: Cell classification based on FOXJ1 intensity. Class 1 (FOXJ1-positive) and class 2 (FOXJ1-negative); magnification factor = ×387. f , PCA of FOXJ1-positive and FOXJ1-negative cell proteomes. g , Heat map of known protein markers for secretory and ciliated cells. Protein levels are z-scored. Asterisks represent imputed data. The marker list was derived from the Human Protein Atlas project and based on literature mining. h , Volcano plot of the pairwise proteomic comparison between FOXJ1-positive and FOXJ1-negative cells. Cell-type-specific marker proteins are highlighted in green and turquoise, and black represents potential novel marker proteins. Significant enriched cell-type-specific proteins are displayed above the black lines (two-sided t -test, FDR < 0.05, s 0 = 0.1, n = 4 biological replicates).

    Article Snippet: Primary antibodies targeting FOXJ1 (mouse, dilution 1:200, 14-9965-80, Invitrogen) and EpCAM (rabbit, dilution 1:200, 14452, Cell Signaling Technology) were diluted in 10% goat serum and incubated overnight at 4 °C in a humidified chamber.

    Techniques: Microscopy, Immunofluorescence, Staining, Marker, Derivative Assay

    a Dot plot showing relative FAP expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an EpCAM staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).

    Journal: British Journal of Cancer

    Article Title: Fibroblast activation protein identifies Consensus Molecular Subtype 4 in colorectal cancer and allows its detection by 68 Ga-FAPI-PET imaging

    doi: 10.1038/s41416-022-01748-z

    Figure Lengend Snippet: a Dot plot showing relative FAP expression levels in primary CRC tumours in the TCGA cohort ( n = 592) versus primary tumours with peritoneal involvement ( n = 35) and the peritoneal metastases derived from them ( n = 59). b Dot plot showing FAP expression levels in peritoneal metastases and their matched primary tumours. CMS subtypes are colour-coded. Tissue type is annotated by shape. c Scatter plot showing the correlation of FAP mRNA levels with CMS4 probabilities of all tumours in the primary tumour/peritoneal metastasis cohort. d Immunohistochemistry for FAP expression in peritoneal metastasis. See Supplementary Fig. for a comprehensive overview of FAP protein expression in peritoneal metastases. e Boxplot of FAP expression quantification on IHC slides of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). FAP expression is measured as the number of FAP-positive cells and corrected for tumour size using an EpCAM staining of the sequential slide. f Boxplot of the percentage of FAP-positive stromal cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19). g Boxplot of the percentage of FAP-positive tumour cells of colorectal liver metastasis (CRLM, n = 21) and peritoneal metastasis (PM, n = 19).

    Article Snippet: Sections were incubated overnight at 4 C with one of the following primary antibodies: anti-FAP (ab207178 Abcam, dilution 1:400), anti-EpCAM (CS 14452 Cell Signaling, dilution 1:200), anti-ZEB1 (HPA027524 Sigma, 1:500) or anti-HTR2B (HPA012867 Sigma, 1:75).

    Techniques: Expressing, Derivative Assay, Immunohistochemistry, Staining

    a Immunohistochemistry for FAP on peritoneal metastases tissue that was resected one year prior to FAPI-PET imaging. b Immunohistochemistry of EpCAM-positive tumour cells in the peritoneum show a strong ZEB1 and HTR2B staining. c FAPI-PET image shows tracer accumulation in pelvic peritoneal depositions and along the abdominal peritoneal lining (white arrows). See also Supplementary Fig. . d FAPI-PET image (same coronal section, same scaling)) after 2 months of chemotherapy shows a reduction of FAPI tracer uptake in the pelvic lesion and the serosal lesion and a complete loss of diffuse FAPI tracer uptake along the peritoneum.

    Journal: British Journal of Cancer

    Article Title: Fibroblast activation protein identifies Consensus Molecular Subtype 4 in colorectal cancer and allows its detection by 68 Ga-FAPI-PET imaging

    doi: 10.1038/s41416-022-01748-z

    Figure Lengend Snippet: a Immunohistochemistry for FAP on peritoneal metastases tissue that was resected one year prior to FAPI-PET imaging. b Immunohistochemistry of EpCAM-positive tumour cells in the peritoneum show a strong ZEB1 and HTR2B staining. c FAPI-PET image shows tracer accumulation in pelvic peritoneal depositions and along the abdominal peritoneal lining (white arrows). See also Supplementary Fig. . d FAPI-PET image (same coronal section, same scaling)) after 2 months of chemotherapy shows a reduction of FAPI tracer uptake in the pelvic lesion and the serosal lesion and a complete loss of diffuse FAPI tracer uptake along the peritoneum.

    Article Snippet: Sections were incubated overnight at 4 C with one of the following primary antibodies: anti-FAP (ab207178 Abcam, dilution 1:400), anti-EpCAM (CS 14452 Cell Signaling, dilution 1:200), anti-ZEB1 (HPA027524 Sigma, 1:500) or anti-HTR2B (HPA012867 Sigma, 1:75).

    Techniques: Immunohistochemistry, Imaging, Staining