sod2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc sod2
    SA attenuates oxidative stress caused by 6-OHDA-induced neurotoxicity in SH-SY5Y neuroblastoma cells. Representative photograph of DCFH-DA staining assay ( A ) and fluorescence intensity ( B ) in cells treated with 6-OHDA for 24 h with or without 100, 200, and 400 μM SA pretreatment for 24 h. ( C ) Western blot analysis was performed to measure superoxide dismutase1 (SOD 1), SOD 2, and catalase protein expression in SA-pretreated/6-OHDA-treated cells. The expression levels of the SOD 1 ( D ), <t>SOD</t> <t>2</t> ( E ), and catalase ( F ) proteins were quantified using HD imaging software. β-actin was used as the loading control. Western blot analysis was performed in triplicate with three independent samples. All data are reported as the mean ± standard error of the mean (SEM). Significance was determined via a one-way ANOVA coupled with Bonferroni’s post hoc test. ## p < 0.01 and ### p < 0.001 vs. control group. ** p < 0.01 and *** p < 0.001 vs. 6-OHDA-only group. Cont, control; SA, sinapic acid; 6-OHDA, 6-hydroxydopamine; SOD, superoxide dismutase. Scale bar, 100 μm.
    Sod2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sod2/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
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    sod2 - by Bioz Stars, 2023-02
    97/100 stars

    Images

    1) Product Images from "Sinapic Acid Protects SH-SY5Y Human Neuroblastoma Cells against 6-Hydroxydopamine-Induced Neurotoxicity"

    Article Title: Sinapic Acid Protects SH-SY5Y Human Neuroblastoma Cells against 6-Hydroxydopamine-Induced Neurotoxicity

    Journal: Biomedicines

    doi: 10.3390/biomedicines9030295

    SA attenuates oxidative stress caused by 6-OHDA-induced neurotoxicity in SH-SY5Y neuroblastoma cells. Representative photograph of DCFH-DA staining assay ( A ) and fluorescence intensity ( B ) in cells treated with 6-OHDA for 24 h with or without 100, 200, and 400 μM SA pretreatment for 24 h. ( C ) Western blot analysis was performed to measure superoxide dismutase1 (SOD 1), SOD 2, and catalase protein expression in SA-pretreated/6-OHDA-treated cells. The expression levels of the SOD 1 ( D ), SOD 2 ( E ), and catalase ( F ) proteins were quantified using HD imaging software. β-actin was used as the loading control. Western blot analysis was performed in triplicate with three independent samples. All data are reported as the mean ± standard error of the mean (SEM). Significance was determined via a one-way ANOVA coupled with Bonferroni’s post hoc test. ## p < 0.01 and ### p < 0.001 vs. control group. ** p < 0.01 and *** p < 0.001 vs. 6-OHDA-only group. Cont, control; SA, sinapic acid; 6-OHDA, 6-hydroxydopamine; SOD, superoxide dismutase. Scale bar, 100 μm.
    Figure Legend Snippet: SA attenuates oxidative stress caused by 6-OHDA-induced neurotoxicity in SH-SY5Y neuroblastoma cells. Representative photograph of DCFH-DA staining assay ( A ) and fluorescence intensity ( B ) in cells treated with 6-OHDA for 24 h with or without 100, 200, and 400 μM SA pretreatment for 24 h. ( C ) Western blot analysis was performed to measure superoxide dismutase1 (SOD 1), SOD 2, and catalase protein expression in SA-pretreated/6-OHDA-treated cells. The expression levels of the SOD 1 ( D ), SOD 2 ( E ), and catalase ( F ) proteins were quantified using HD imaging software. β-actin was used as the loading control. Western blot analysis was performed in triplicate with three independent samples. All data are reported as the mean ± standard error of the mean (SEM). Significance was determined via a one-way ANOVA coupled with Bonferroni’s post hoc test. ## p < 0.01 and ### p < 0.001 vs. control group. ** p < 0.01 and *** p < 0.001 vs. 6-OHDA-only group. Cont, control; SA, sinapic acid; 6-OHDA, 6-hydroxydopamine; SOD, superoxide dismutase. Scale bar, 100 μm.

    Techniques Used: Staining, Fluorescence, Western Blot, Expressing, Imaging, Software