acat2 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc acat2 antibody
    Acat2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2 antibody/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
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    acat2 antibody - by Bioz Stars, 2023-01
    92/100 stars

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    acat2 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc acat2 antibody
    Acat2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2 antibody/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
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    acat2 antibody - by Bioz Stars, 2023-01
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    acat2 13294s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc acat2 13294s
    Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and <t>ACAT2</t> in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group
    Acat2 13294s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2 13294s/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acat2 13294s - by Bioz Stars, 2023-01
    92/100 stars

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    1) Product Images from "Pioglitazone prevents cholesterol gallstone formation through the regulation of cholesterol homeostasis in guinea pigs with a lithogenic diet"

    Article Title: Pioglitazone prevents cholesterol gallstone formation through the regulation of cholesterol homeostasis in guinea pigs with a lithogenic diet

    Journal: Lipids in Health and Disease

    doi: 10.1186/s12944-019-1159-4

    Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and ACAT2 in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group
    Figure Legend Snippet: Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and ACAT2 in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group

    Techniques Used: Western Blot

    acat2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc acat2
    Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and <t>ACAT2</t> ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean
    Acat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
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    1) Product Images from "Systems analysis of intracellular pH vulnerabilities for cancer therapy"

    Article Title: Systems analysis of intracellular pH vulnerabilities for cancer therapy

    Journal: Nature Communications

    doi: 10.1038/s41467-018-05261-x

    Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and ACAT2 ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean
    Figure Legend Snippet: Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and ACAT2 ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean

    Techniques Used: Inhibition, Transfection, Amplification, Viability Assay, Standard Deviation

    jbc c110 132944 hitoshi kawaguchi  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jbc c110 132944 hitoshi kawaguchi
    Jbc C110 132944 Hitoshi Kawaguchi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jbc c110 132944 hitoshi kawaguchi  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jbc c110 132944 hitoshi kawaguchi
    Jbc C110 132944 Hitoshi Kawaguchi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc acat2 antibody
    Acat2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2 antibody/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc acat2 13294s
    Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and <t>ACAT2</t> in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group
    Acat2 13294s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2 13294s/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc acat2
    Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and <t>ACAT2</t> ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean
    Acat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acat2/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc jbc c110 132944 hitoshi kawaguchi
    Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and <t>ACAT2</t> ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean
    Jbc C110 132944 Hitoshi Kawaguchi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and ACAT2 in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group

    Journal: Lipids in Health and Disease

    Article Title: Pioglitazone prevents cholesterol gallstone formation through the regulation of cholesterol homeostasis in guinea pigs with a lithogenic diet

    doi: 10.1186/s12944-019-1159-4

    Figure Lengend Snippet: Pioglitazone increases ABCG5 and ABCG8 and decreases NPC1L1 and ACAT2 in intestine. a Western blot analysis of ABCG5, ABCG8, NPC1L1 and ACAT2 protein; b Quantification of ABCG5 expressed as the ratio (as a percentage) of control; c Quantification of ABCG8 expressed as the ratio (as a percentage) of control; d Quantification of NPC1L1 expressed as the ratio (as a percentage) of control; e Quantification of ACAT2 expressed as the ratio (as a percentage) of control. Values shown are means ±SD, n = 4. *p < 0.05 and **p < 0.01 versus LD group

    Article Snippet: The antibodies of ACAT2 (13294S) and PPARγ (2443S) were from Cell Signaling Technology Inc. (Boston, USA).

    Techniques: Western Blot

    Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and ACAT2 ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean

    Journal: Nature Communications

    Article Title: Systems analysis of intracellular pH vulnerabilities for cancer therapy

    doi: 10.1038/s41467-018-05261-x

    Figure Lengend Snippet: Validation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. a pHi measurements of naïve and acid-adapted (AA) breast cancer MCF7 cells, under normoxia and following the inhibition of NHE1 , by cariporide treatment. For pHi measurments at least 30 cells were analyzed. b Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. qPCR was repeated at least three times with three replicates. c The effect of gene inhibition in normal and low extracellular pH (pHe) shown for naïve and AA MCF7 breast cancer cells. Similar color code to Fig. is applied. At low pHe where the lowest pHi was obtained there is a large reduction in the viability of cells. In AA cells, only the selective and pH-specific targets ( GAPDH , GPI , and ACAT2 ) achieve amplified anti-proliferative effects following NHE1 inhibition, despite the smaller reduction in the pHi of these cells. PFAS , a selective but not pH-specific target, is similar to control cells following NHE1 inhibition. Knockdown of RPIA had a weak effect in naïve cells and no/opposite effects in AA cells. The viability assay was done three times with four replicates each time. The bars depict the mean and the error bars depict the standard deviation of the mean

    Article Snippet: Membranes were incubated with primary antibodies against GAPDH (Cat# 2118 Cell Signaling, 1:2000), GPI (ab68643, Abcam, 1:1000), ACAT2 (Cat# 13294s Cell Signaling 1:1000), RPIA (ab181235, abcam, 1:500), PFAS (PA554628, Thermofisher, 1:200), MCT1 (sc-365501, Santa Cruz Biotechnology, 1:500), MCT4 (sc-376140, Santa Cruz Biotechnology, 1:500), and β-Actin (A5441, Sigma, 1:6000).

    Techniques: Inhibition, Transfection, Amplification, Viability Assay, Standard Deviation