cd147 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd147 antibody
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Cd147 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147 antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd147 antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness"

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    Journal: BioMed Research International

    doi: 10.1155/2020/7035847

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Figure Legend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Techniques Used: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
    Figure Legend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Techniques Used: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
    Figure Legend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Techniques Used: Expressing

    cd147 antibody  (Cell Signaling Technology Inc)


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  • 94

    Structured Review

    Cell Signaling Technology Inc cd147 antibody
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Cd147 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147 antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd147 antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness"

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    Journal: BioMed Research International

    doi: 10.1155/2020/7035847

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Figure Legend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Techniques Used: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
    Figure Legend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Techniques Used: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
    Figure Legend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Techniques Used: Expressing

    cd147  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc cd147
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd147 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness"

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    Journal: BioMed Research International

    doi: 10.1155/2020/7035847

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
    Figure Legend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Techniques Used: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
    Figure Legend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Techniques Used: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
    Figure Legend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Techniques Used: Expressing

    antibodies against emmprin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against emmprin
    Decrease in invasion activities of SaOS-2 cells transfected by <t>EMMPRIN</t> siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, <t>the</t> <t>membranes</t> were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
    Antibodies Against Emmprin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against emmprin/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against emmprin - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "EMMPRIN expression is associated with metastatic progression in osteosarcoma"

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    Journal: BMC Cancer

    doi: 10.1186/s12885-021-08774-9

    Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
    Figure Legend Snippet: Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Techniques Used: Transfection, Incubation, Light Microscopy, Matrigel Assay, Invasion Assay

    EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40
    Figure Legend Snippet: EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Techniques Used: Injection, shRNA, Transfection, Staining, Western Blot, Microscopy

    rabbit monoclonal antibodies against cd147  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc rabbit monoclonal antibodies against cd147
    mRNA and protein expression levels of <t>CD147</t> in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Rabbit Monoclonal Antibodies Against Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against cd147 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway"

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2020.9058

    mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, shRNA

    CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Incubation, Cell Counting, Standard Deviation, shRNA

    CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Migration, Standard Deviation, shRNA

    CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
    Figure Legend Snippet: CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Techniques Used: Expressing, Marker, Western Blot, Standard Deviation, shRNA

    CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.
    Figure Legend Snippet: CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Techniques Used: Expressing, Western Blot, Standard Deviation, shRNA

    13287s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 13287s
    KEY RESOURCES TABLE
    13287s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/13287s/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    13287s - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism"

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.10.100

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software

    rabbit anti human cd147  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti human cd147
    Rabbit Anti Human Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human cd147 - by Bioz Stars, 2023-01
    94/100 stars

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    cd147  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cd147
    Transwell migration assays of MM1S-luc cells incubated under different conditions: ( a ) increased concentrations of recombinant eCyPA.; ( b ) medium alone or BMEC-60 cells lentivirally transduced with Control-shRNA or shRNAs against CyPA (CyPA-shRNA). Migration data was normalized based on data of medium alone. Results are means ± SD for assays performed in triplicate. Statistical significance of differences between groups was determined by unpaired Student's t-test. ( c ) Immunoblot of total protein extracts from H929 and MM1S cells incubated in the absence (-) or presence (+) of eCyPA at 50ng per ml. Xenogen data ( d ), time course ( e ), and histologic analysis ( f ) of MM1S-luc cell growth within scaffolds coated with BMEC-60 lentivirally transduced with ControlshRNAs or CyPA-shRNA. Bars: Top and midle 20μm, Bottom 100μm. The results of one representative of three independent experiment is shown Xenogen data ( g ), time course ( h ), and histologic analysis ( i ) of cell growth of MM1S-luc transduced with Control-shRNA or <t>CD147-shRNA</t> within empty scaffolds or scaffolds coated with BMEC-60 cells. Bar: 100μm. Statistical analyses of tumor burden were done using factorial analysis in SPSS 13.0. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).
    Cd147, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd147/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd147 - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "The Cyclophilin A-CD147 complex promotes bone marrow colonization of B-cell malignancies: implications for therapy"

    Article Title: The Cyclophilin A-CD147 complex promotes bone marrow colonization of B-cell malignancies: implications for therapy

    Journal: Nature medicine

    doi: 10.1038/nm.3867

    Transwell migration assays of MM1S-luc cells incubated under different conditions: ( a ) increased concentrations of recombinant eCyPA.; ( b ) medium alone or BMEC-60 cells lentivirally transduced with Control-shRNA or shRNAs against CyPA (CyPA-shRNA). Migration data was normalized based on data of medium alone. Results are means ± SD for assays performed in triplicate. Statistical significance of differences between groups was determined by unpaired Student's t-test. ( c ) Immunoblot of total protein extracts from H929 and MM1S cells incubated in the absence (-) or presence (+) of eCyPA at 50ng per ml. Xenogen data ( d ), time course ( e ), and histologic analysis ( f ) of MM1S-luc cell growth within scaffolds coated with BMEC-60 lentivirally transduced with ControlshRNAs or CyPA-shRNA. Bars: Top and midle 20μm, Bottom 100μm. The results of one representative of three independent experiment is shown Xenogen data ( g ), time course ( h ), and histologic analysis ( i ) of cell growth of MM1S-luc transduced with Control-shRNA or CD147-shRNA within empty scaffolds or scaffolds coated with BMEC-60 cells. Bar: 100μm. Statistical analyses of tumor burden were done using factorial analysis in SPSS 13.0. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).
    Figure Legend Snippet: Transwell migration assays of MM1S-luc cells incubated under different conditions: ( a ) increased concentrations of recombinant eCyPA.; ( b ) medium alone or BMEC-60 cells lentivirally transduced with Control-shRNA or shRNAs against CyPA (CyPA-shRNA). Migration data was normalized based on data of medium alone. Results are means ± SD for assays performed in triplicate. Statistical significance of differences between groups was determined by unpaired Student's t-test. ( c ) Immunoblot of total protein extracts from H929 and MM1S cells incubated in the absence (-) or presence (+) of eCyPA at 50ng per ml. Xenogen data ( d ), time course ( e ), and histologic analysis ( f ) of MM1S-luc cell growth within scaffolds coated with BMEC-60 lentivirally transduced with ControlshRNAs or CyPA-shRNA. Bars: Top and midle 20μm, Bottom 100μm. The results of one representative of three independent experiment is shown Xenogen data ( g ), time course ( h ), and histologic analysis ( i ) of cell growth of MM1S-luc transduced with Control-shRNA or CD147-shRNA within empty scaffolds or scaffolds coated with BMEC-60 cells. Bar: 100μm. Statistical analyses of tumor burden were done using factorial analysis in SPSS 13.0. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).

    Techniques Used: Migration, Incubation, Recombinant, Transduction, shRNA, Western Blot

    ( a ) Time course of Xenogen imaging of MM1S-luc cell growth in scaffolds implanted inCB17.Cg-PrkdcscidLystbg-J-Crl mice and treated with local injections of isotype control or anti-CD147 Abs. Xenogen data ( b ) of MM1S-luc cell growth within scaffolds coated with BMEC-60 cells and implanted subcutaneously in CB17.Cg- Prkdc scid Lyst bg-J -Crl mice. Groups of 4 mice were subsequently treated with either isotype Ab or anti-CD147 Ab, and tumor growth within the scaffolds was evaluated by Xenogen imaging every five days. ( c ) Left panel, Immunofluorescence analysis of CD147 expression in MM plasma cells from BM (top) and PB (bottom) from one person with MM (Case 1). Right panel, Immunofluorescence analysis of CD147 expression in normal plasma cells from BM (top) and lymph node (LN) (bottom) in two different normal donors (Case 3 and 4). Bars: 5μm. ( d ) Proposed model of BM homing of MM cells based on eCyPA secreted by BMECs and on CD147 expression by MM cells. Statistical analysis of tumor burden were done using factorial analysis in SPSS 13.0. (*P<0.05, **P<0.01).
    Figure Legend Snippet: ( a ) Time course of Xenogen imaging of MM1S-luc cell growth in scaffolds implanted inCB17.Cg-PrkdcscidLystbg-J-Crl mice and treated with local injections of isotype control or anti-CD147 Abs. Xenogen data ( b ) of MM1S-luc cell growth within scaffolds coated with BMEC-60 cells and implanted subcutaneously in CB17.Cg- Prkdc scid Lyst bg-J -Crl mice. Groups of 4 mice were subsequently treated with either isotype Ab or anti-CD147 Ab, and tumor growth within the scaffolds was evaluated by Xenogen imaging every five days. ( c ) Left panel, Immunofluorescence analysis of CD147 expression in MM plasma cells from BM (top) and PB (bottom) from one person with MM (Case 1). Right panel, Immunofluorescence analysis of CD147 expression in normal plasma cells from BM (top) and lymph node (LN) (bottom) in two different normal donors (Case 3 and 4). Bars: 5μm. ( d ) Proposed model of BM homing of MM cells based on eCyPA secreted by BMECs and on CD147 expression by MM cells. Statistical analysis of tumor burden were done using factorial analysis in SPSS 13.0. (*P<0.05, **P<0.01).

    Techniques Used: Imaging, Immunofluorescence, Expressing

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    Cell Signaling Technology Inc cd147 antibody
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
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    Cell Signaling Technology Inc cd147
    Expressions of CyPA and <t>CD147</t> are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.
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    Cell Signaling Technology Inc antibodies against emmprin
    Decrease in invasion activities of SaOS-2 cells transfected by <t>EMMPRIN</t> siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, <t>the</t> <t>membranes</t> were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm
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    mRNA and protein expression levels of <t>CD147</t> in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.
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    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Article Snippet: After the blockade of nonspecific binding, tissue sections were incubated with the Cyclophilin A antibody (cat. no. 5360s; 1 : 150 dilution; Santa Cruz Biotechnology, Inc.) and CD147 antibody (cat. no. 13287; 1 : 400 dilution; Cell Signaling Technology, Inc.) for overnight at 4 centigrade.

    Techniques: Expressing

    Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns stands for no significance.

    Article Snippet: The primary antibodies used were as follows: Cyclophilin A (cat. no. 5360), CD147 (cat. no. 13287) (all from Cell Signaling Technology, Inc., Danvers, MA, USA, and used at a dilution of 1 : 1,000) and β -actin (cat. no. SC-130300, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: Expressing

    Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

    Article Snippet: The primary antibodies used were as follows: Cyclophilin A (cat. no. 5360), CD147 (cat. no. 13287) (all from Cell Signaling Technology, Inc., Danvers, MA, USA, and used at a dilution of 1 : 1,000) and β -actin (cat. no. SC-130300, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.

    Article Snippet: The primary antibodies used were as follows: Cyclophilin A (cat. no. 5360), CD147 (cat. no. 13287) (all from Cell Signaling Technology, Inc., Danvers, MA, USA, and used at a dilution of 1 : 1,000) and β -actin (cat. no. SC-130300, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: EdU Assay, Inhibition, Flow Cytometry

    Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Journal: BioMed Research International

    Article Title: Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

    doi: 10.1155/2020/7035847

    Figure Lengend Snippet: Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.

    Article Snippet: The primary antibodies used were as follows: Cyclophilin A (cat. no. 5360), CD147 (cat. no. 13287) (all from Cell Signaling Technology, Inc., Danvers, MA, USA, and used at a dilution of 1 : 1,000) and β -actin (cat. no. SC-130300, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: Expressing

    Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Journal: BMC Cancer

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    doi: 10.1186/s12885-021-08774-9

    Figure Lengend Snippet: Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

    Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

    Techniques: Transfection, Incubation, Light Microscopy, Matrigel Assay, Invasion Assay

    EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Journal: BMC Cancer

    Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

    doi: 10.1186/s12885-021-08774-9

    Figure Lengend Snippet: EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

    Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

    Techniques: Injection, shRNA, Transfection, Staining, Western Blot, Microscopy

    mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, shRNA

    CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Incubation, Cell Counting, Standard Deviation, shRNA

    CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Migration, Standard Deviation, shRNA

    CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Marker, Western Blot, Standard Deviation, shRNA

    CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Journal: Experimental and Therapeutic Medicine

    Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

    doi: 10.3892/etm.2020.9058

    Figure Lengend Snippet: CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

    Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

    Techniques: Expressing, Western Blot, Standard Deviation, shRNA

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism

    doi: 10.1016/j.celrep.2018.10.100

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-human Basigin (E1S1V) , Cell Signaling Technology , Cat. # 13287S.

    Techniques: Recombinant, Protease Inhibitor, Mutagenesis, Lysis, Transfection, Enzyme-linked Immunosorbent Assay, Lactate Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, RNA Sequencing Assay, Binding Assay, Software