mrp4 abcc4  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mrp4 abcc4
    Primer sequences.
    Mrp4 Abcc4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrp4 abcc4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mrp4 abcc4 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway"

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2022/3032407

    Primer sequences.
    Figure Legend Snippet: Primer sequences.

    Techniques Used: Sequencing

    Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.
    Figure Legend Snippet: Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.
    Figure Legend Snippet: Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Techniques Used: Expressing, In Vivo, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.
    Figure Legend Snippet: Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Techniques Used: Expressing, In Vivo, Immunofluorescence, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    mrp4 abcc4  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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  • 94

    Structured Review

    Cell Signaling Technology Inc mrp4 abcc4
    Primer sequences.
    Mrp4 Abcc4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrp4 abcc4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mrp4 abcc4 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway"

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2022/3032407

    Primer sequences.
    Figure Legend Snippet: Primer sequences.

    Techniques Used: Sequencing

    Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.
    Figure Legend Snippet: Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.
    Figure Legend Snippet: Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Techniques Used: Expressing, In Vivo, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.
    Figure Legend Snippet: Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Techniques Used: Expressing, In Vivo, Immunofluorescence, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    mrp4 abcc4  (Cell Signaling Technology Inc)


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  • 94

    Structured Review

    Cell Signaling Technology Inc mrp4 abcc4
    Intermittent fasting (IF) suggests reversal of DNA methylation status in the promoter region under chronic cerebral hypoperfusion (CCH). The methylation status of the differentially methylated genes were compared to the methylation levels of the respective time specific IF Sham group. Venn diagram of differentially methylated genes across all gene regions in the AL BCAS mice compared to AL Sham mice (A) and in the IF BCAS mice compared to AL BCAS mice (B) with respect to their corresponding Sham groups. The breakdown of specific methylation trends of differentially methylated genes were represented in Venn diagrams. A total of 9 hypermethylated genes and 7 hypomethylated genes were identified to be overlapping across all three timepoints in the AL BCAS mice and a total of 3 hypermethylated and 18 hypomethylated genes were identified to be overlapping across all three timepoints in the IF BCAS mice. (C) Representative immunoblots and quantification of overlapping differentially methylated genes to validate findings in DNA methylation sequencing. This includes protein abundance of ACTN1 in AL CCH compared to AL Sham mice, GNAS in AL CCH compared to AL Sham mice, <t>MRP4</t> in AL CCH compared to AL Sham mice and IF CCH compared to IF Sham mice. Vinculin and β-actin were used as loading controls. Data are represented as mean ± SD. n=5-7 mice in each experimental group. Unpaired t-test, Bonferroni correction, *p<0.05 compared to ALB30D. Analysing specific differentially methylated genes in the promoter region by presenting the spread in the form of a Venn diagram in the (D) AL BCAS mice compared to the AL Sham mice and (E) IF BCAS mice compared to the AL BCAS mice. (F) 5 differentially methylated genes were identified to be overlapping across three different timepoints in the AL BCAS mice. The methylation levels of the 5 genes were illustrated in the form of a heatmap to show its temporal regulation which was present in Ddx3y, Gnas and Pax6. (G) 4 differentially methylated genes were identified to be overlapping across the three different timepoints in the promoter region of the IF BCAS mice. The methylation levels of the 4 genes were illustrated in the form of a heatmap to show the temporal regulation which was present in Zrsr1, Gng10, Actg1 and Speg.
    Mrp4 Abcc4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrp4 abcc4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mrp4 abcc4 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "Time-restricted feeding modulates the DNA methylation landscape, attenuates hallmark neuropathology and cognitive impairment in a mouse model of vascular dementia"

    Article Title: Time-restricted feeding modulates the DNA methylation landscape, attenuates hallmark neuropathology and cognitive impairment in a mouse model of vascular dementia

    Journal: Theranostics

    doi: 10.7150/thno.71815

    Intermittent fasting (IF) suggests reversal of DNA methylation status in the promoter region under chronic cerebral hypoperfusion (CCH). The methylation status of the differentially methylated genes were compared to the methylation levels of the respective time specific IF Sham group. Venn diagram of differentially methylated genes across all gene regions in the AL BCAS mice compared to AL Sham mice (A) and in the IF BCAS mice compared to AL BCAS mice (B) with respect to their corresponding Sham groups. The breakdown of specific methylation trends of differentially methylated genes were represented in Venn diagrams. A total of 9 hypermethylated genes and 7 hypomethylated genes were identified to be overlapping across all three timepoints in the AL BCAS mice and a total of 3 hypermethylated and 18 hypomethylated genes were identified to be overlapping across all three timepoints in the IF BCAS mice. (C) Representative immunoblots and quantification of overlapping differentially methylated genes to validate findings in DNA methylation sequencing. This includes protein abundance of ACTN1 in AL CCH compared to AL Sham mice, GNAS in AL CCH compared to AL Sham mice, MRP4 in AL CCH compared to AL Sham mice and IF CCH compared to IF Sham mice. Vinculin and β-actin were used as loading controls. Data are represented as mean ± SD. n=5-7 mice in each experimental group. Unpaired t-test, Bonferroni correction, *p<0.05 compared to ALB30D. Analysing specific differentially methylated genes in the promoter region by presenting the spread in the form of a Venn diagram in the (D) AL BCAS mice compared to the AL Sham mice and (E) IF BCAS mice compared to the AL BCAS mice. (F) 5 differentially methylated genes were identified to be overlapping across three different timepoints in the AL BCAS mice. The methylation levels of the 5 genes were illustrated in the form of a heatmap to show its temporal regulation which was present in Ddx3y, Gnas and Pax6. (G) 4 differentially methylated genes were identified to be overlapping across the three different timepoints in the promoter region of the IF BCAS mice. The methylation levels of the 4 genes were illustrated in the form of a heatmap to show the temporal regulation which was present in Zrsr1, Gng10, Actg1 and Speg.
    Figure Legend Snippet: Intermittent fasting (IF) suggests reversal of DNA methylation status in the promoter region under chronic cerebral hypoperfusion (CCH). The methylation status of the differentially methylated genes were compared to the methylation levels of the respective time specific IF Sham group. Venn diagram of differentially methylated genes across all gene regions in the AL BCAS mice compared to AL Sham mice (A) and in the IF BCAS mice compared to AL BCAS mice (B) with respect to their corresponding Sham groups. The breakdown of specific methylation trends of differentially methylated genes were represented in Venn diagrams. A total of 9 hypermethylated genes and 7 hypomethylated genes were identified to be overlapping across all three timepoints in the AL BCAS mice and a total of 3 hypermethylated and 18 hypomethylated genes were identified to be overlapping across all three timepoints in the IF BCAS mice. (C) Representative immunoblots and quantification of overlapping differentially methylated genes to validate findings in DNA methylation sequencing. This includes protein abundance of ACTN1 in AL CCH compared to AL Sham mice, GNAS in AL CCH compared to AL Sham mice, MRP4 in AL CCH compared to AL Sham mice and IF CCH compared to IF Sham mice. Vinculin and β-actin were used as loading controls. Data are represented as mean ± SD. n=5-7 mice in each experimental group. Unpaired t-test, Bonferroni correction, *p<0.05 compared to ALB30D. Analysing specific differentially methylated genes in the promoter region by presenting the spread in the form of a Venn diagram in the (D) AL BCAS mice compared to the AL Sham mice and (E) IF BCAS mice compared to the AL BCAS mice. (F) 5 differentially methylated genes were identified to be overlapping across three different timepoints in the AL BCAS mice. The methylation levels of the 5 genes were illustrated in the form of a heatmap to show its temporal regulation which was present in Ddx3y, Gnas and Pax6. (G) 4 differentially methylated genes were identified to be overlapping across the three different timepoints in the promoter region of the IF BCAS mice. The methylation levels of the 4 genes were illustrated in the form of a heatmap to show the temporal regulation which was present in Zrsr1, Gng10, Actg1 and Speg.

    Techniques Used: DNA Methylation Assay, Methylation, Western Blot, Sequencing

    anti abcc4 d2q2o rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti abcc4 d2q2o rabbit mab
    I-CBP 112 decreased the expression of ABCC1, ABCC3, <t>ABCC4,</t> ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.
    Anti Abcc4 D2q2o Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti abcc4 d2q2o rabbit mab/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti abcc4 d2q2o rabbit mab - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "CBP/p300 Bromodomain Inhibitor–I–CBP112 Declines Transcription of the Key ABC Transporters and Sensitizes Cancer Cells to Chemotherapy Drugs"

    Article Title: CBP/p300 Bromodomain Inhibitor–I–CBP112 Declines Transcription of the Key ABC Transporters and Sensitizes Cancer Cells to Chemotherapy Drugs

    Journal: Cancers

    doi: 10.3390/cancers13184614

    I-CBP 112 decreased the expression of ABCC1, ABCC3, ABCC4, ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.
    Figure Legend Snippet: I-CBP 112 decreased the expression of ABCC1, ABCC3, ABCC4, ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.

    Techniques Used: Expressing, Incubation, Real-time Polymerase Chain Reaction, Western Blot, Confocal Microscopy, Staining

    mrp4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mrp4
    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, <t>MRP4,</t> Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Mrp4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrp4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mrp4 - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer"

    Article Title: The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer

    Journal: Molecular Medicine

    doi: 10.1186/s10020-020-00174-2

    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, MRP4, Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Figure Legend Snippet: DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, MRP4, Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Techniques Used: Activity Assay, Transplantation Assay, Migration, Expressing, Western Blot

    Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1 , MRP4 , Snail , Slug , 12LOX , COX1 , and CYP2J2 . b SP1 binding sequences in the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). c ChIP assay of SP1 binding on the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). d, e The mRNA ( d ) and protein ( e ) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in ( d ) are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and * P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P -values of 0.05 or less were considered significant
    Figure Legend Snippet: Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1 , MRP4 , Snail , Slug , 12LOX , COX1 , and CYP2J2 . b SP1 binding sequences in the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). c ChIP assay of SP1 binding on the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). d, e The mRNA ( d ) and protein ( e ) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in ( d ) are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and * P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P -values of 0.05 or less were considered significant

    Techniques Used: Binding Assay, Expressing

    Inhibition of SP1 or 12LOX dampens metastasis and DDP-resistance of SKOV3-R cells in vitro. a-d After incubation with Bai (20 μM) or MA (30 nM) for 24 h, migration and invasion were tested by wound-healing assays ( a ) and transwell assays ( b ); the cell viability percentages were measured by CCK-8 assay ( c, d ). siNC denoted SP1 siRNA negative control, si-SP1 denoted SP1 siRNA. e-g After SKOV3-R cells were treated with these siRNAs, the gene expression levels of SP1 was evaluated by qPCR ( e ) and migration and invasion were tested by wound-healing assays ( f ) and transwell assays ( g ). h-j After administration with Bai (20 μM) or MA (30 nM) for 24 h, the heatmap for gene expression of MRP1 , MRP4 , SP1 , 12LOX , Slug and Snail ( h ) and the protein level of MRP1, MRP4, Slug and Snail were evaluated by western blotting ( i,j ). Data are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001
    Figure Legend Snippet: Inhibition of SP1 or 12LOX dampens metastasis and DDP-resistance of SKOV3-R cells in vitro. a-d After incubation with Bai (20 μM) or MA (30 nM) for 24 h, migration and invasion were tested by wound-healing assays ( a ) and transwell assays ( b ); the cell viability percentages were measured by CCK-8 assay ( c, d ). siNC denoted SP1 siRNA negative control, si-SP1 denoted SP1 siRNA. e-g After SKOV3-R cells were treated with these siRNAs, the gene expression levels of SP1 was evaluated by qPCR ( e ) and migration and invasion were tested by wound-healing assays ( f ) and transwell assays ( g ). h-j After administration with Bai (20 μM) or MA (30 nM) for 24 h, the heatmap for gene expression of MRP1 , MRP4 , SP1 , 12LOX , Slug and Snail ( h ) and the protein level of MRP1, MRP4, Slug and Snail were evaluated by western blotting ( i,j ). Data are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001

    Techniques Used: Inhibition, In Vitro, Incubation, Migration, CCK-8 Assay, Negative Control, Expressing, Western Blot

    rabbit anti mrp4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti mrp4
    Rabbit Anti Mrp4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti abcc4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti abcc4
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    mrp4 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mrp4 antibodies
    Bcl2 and <t>MRP4</t> are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.
    Mrp4 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma"

    Article Title: Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2019.00027

    Bcl2 and MRP4 are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.
    Figure Legend Snippet: Bcl2 and MRP4 are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.

    Techniques Used: Binding Assay, Expressing, Luciferase, Reporter Assay, Transfection

    Enhancement of Bcl2 and MRP4 partially reversed the suppressive effects of up-regulation of miR-125a-5p on paclitaxel resistance in EC cells. (A) The expression of Bcl2 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (B) The expression of MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (C,D) : The IC50 of paclitaxel in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; ## P < 0.01 vs. agomiR-125a-5p + pUC group.
    Figure Legend Snippet: Enhancement of Bcl2 and MRP4 partially reversed the suppressive effects of up-regulation of miR-125a-5p on paclitaxel resistance in EC cells. (A) The expression of Bcl2 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (B) The expression of MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (C,D) : The IC50 of paclitaxel in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; ## P < 0.01 vs. agomiR-125a-5p + pUC group.

    Techniques Used: Expressing

    anti mrp4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti mrp4
    ISL pretreatment induced expressions of (A) MRP2, (B) <t>MRP4,</t> and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.
    Anti Mrp4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mechanisms of Triptolide-Induced Hepatotoxicity and Protective Effect of Combined Use of Isoliquiritigenin: Possible Roles of Nrf2 and Hepatic Transporters"

    Article Title: Mechanisms of Triptolide-Induced Hepatotoxicity and Protective Effect of Combined Use of Isoliquiritigenin: Possible Roles of Nrf2 and Hepatic Transporters

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00226

    ISL pretreatment induced expressions of (A) MRP2, (B) MRP4, and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.
    Figure Legend Snippet: ISL pretreatment induced expressions of (A) MRP2, (B) MRP4, and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effects of TP, ISL, or ISL + TP on the protein levels of (A) MRP2, (B) MRP4, and (C) P-gp and the mRNA levels of (D) Bsep and (E) Oatp2 in mouse livers. Cell lysates were analyzed by Western blot and mRNAs by RT-PCR. Data were presented as means ± SE ( n = 5); ∗ P < 0.05 vs. the control group, ∗∗∗ P < 0.001 vs. the control group, # P < 0.05 vs. the TP group, and ### P < 0.001 vs. the TP group.
    Figure Legend Snippet: Effects of TP, ISL, or ISL + TP on the protein levels of (A) MRP2, (B) MRP4, and (C) P-gp and the mRNA levels of (D) Bsep and (E) Oatp2 in mouse livers. Cell lysates were analyzed by Western blot and mRNAs by RT-PCR. Data were presented as means ± SE ( n = 5); ∗ P < 0.05 vs. the control group, ∗∗∗ P < 0.001 vs. the control group, # P < 0.05 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Techniques Used: Western Blot, Reverse Transcription Polymerase Chain Reaction

    anti mrp4 abcc4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti mrp4 abcc4
    Anti Mrp4 Abcc4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit mrp 4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit mrp 4
    Rabbit Mrp 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc mrp4 abcc4
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    I-CBP 112 decreased the expression of ABCC1, ABCC3, <t>ABCC4,</t> ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.
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    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, <t>MRP4,</t> Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, <t>MRP4,</t> Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, <t>MRP4,</t> Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    Bcl2 and <t>MRP4</t> are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.
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    ISL pretreatment induced expressions of (A) MRP2, (B) <t>MRP4,</t> and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.
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    ISL pretreatment induced expressions of (A) MRP2, (B) <t>MRP4,</t> and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.
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    Cell Signaling Technology Inc rabbit mrp 4
    ISL pretreatment induced expressions of (A) MRP2, (B) <t>MRP4,</t> and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.
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    Primer sequences.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    doi: 10.1155/2022/3032407

    Figure Lengend Snippet: Primer sequences.

    Article Snippet: The primary antibodies used were MDR1/ABCB1 (CST, USA), ABCG2 (CST, USA), MRP4/ABCC4 (CST, USA), JNK2 (CST, USA), phospho-SAPK/JNK (CST, USA), and GAPDH (CST, USA).

    Techniques: Sequencing

    Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    doi: 10.1155/2022/3032407

    Figure Lengend Snippet: Effect of ZJW on the expression of the drug resistance-related proteins MDR1/ABCB1, MRP4/ABCC4, and ABCG2 in HCT116/L-OHP cells. (a and b) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 proteins in HCT116 cells and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. (c) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in HCT116 and HCT116/L-OHP cells treated with L-OHP combined with different concentrations of ZJW. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001 compared to HCT116/L.

    Article Snippet: The primary antibodies used were MDR1/ABCB1 (CST, USA), ABCG2 (CST, USA), MRP4/ABCC4 (CST, USA), JNK2 (CST, USA), phospho-SAPK/JNK (CST, USA), and GAPDH (CST, USA).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    doi: 10.1155/2022/3032407

    Figure Lengend Snippet: Effects of ZJW on the JNK signaling pathway, miR-200s, and MALAT1 expression in vivo. (a and d) Immunohistochemistry detection of MRP4 protein in vivo. (b and c) Western blot and real-time PCR assays of JNK and p-JNK levels in vivo. (e and f) Real-time PCR assay of miR-200s levels in vivo. (g) Real-time PCR assay of MALAT1 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Article Snippet: The primary antibodies used were MDR1/ABCB1 (CST, USA), ABCG2 (CST, USA), MRP4/ABCC4 (CST, USA), JNK2 (CST, USA), phospho-SAPK/JNK (CST, USA), and GAPDH (CST, USA).

    Techniques: Expressing, In Vivo, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Zuo Jin Wan Reverses the Resistance of Colorectal Cancer to Oxaliplatin by Regulating the MALAT1/miR-200s/JNK Signaling Pathway

    doi: 10.1155/2022/3032407

    Figure Lengend Snippet: Effect of ZJW on the expression of drug resistance-related proteins in vivo. (a and b) Immunofluorescence detection of MDR1/ABCB1 and ABCG2 proteins in vivo. (c and d) Immunohistochemistry detection of MRP4/ABCC4 protein in vivo. (e and f) Western blot assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. (g) Real-time PCR assay of ABCG2, MDR1/ABCB1, and MRP4/ABCC4 levels in vivo. ∗ , P < 0.05; ∗∗ , P < 0.01; ∗∗∗ , P < 0.001; ∗∗∗∗ , P < 0.0001; ns, no significance compared to MODEL.

    Article Snippet: The primary antibodies used were MDR1/ABCB1 (CST, USA), ABCG2 (CST, USA), MRP4/ABCC4 (CST, USA), JNK2 (CST, USA), phospho-SAPK/JNK (CST, USA), and GAPDH (CST, USA).

    Techniques: Expressing, In Vivo, Immunofluorescence, Immunohistochemistry, Western Blot, Real-time Polymerase Chain Reaction

    I-CBP 112 decreased the expression of ABCC1, ABCC3, ABCC4, ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.

    Journal: Cancers

    Article Title: CBP/p300 Bromodomain Inhibitor–I–CBP112 Declines Transcription of the Key ABC Transporters and Sensitizes Cancer Cells to Chemotherapy Drugs

    doi: 10.3390/cancers13184614

    Figure Lengend Snippet: I-CBP 112 decreased the expression of ABCC1, ABCC3, ABCC4, ABCC5, and ABCC10 in the MDA-MB-231 cell line. ( A – C ) Cells were incubated with 10 µM I-CBP112 for 72 h. ( A ) The mRNA levels of selected ABC transporters compared between the control and I-CBP112-treated cells by real-time PCR. ABC gene expression was normalized to the mRNA levels of ACTB and GAPDH. ( B ) The same transporters were visualized in cell lysates by Western blotting, and H3 was used as a loading control. ( C ) ABC protein level and localization were compared through confocal microscopy using AlexaFluor488-conjugated secondary antibody (green). DNA was stained with DAPI (blue). ( A ) Bars represent mean ± standard error of the mean (SEM). The differences between 2 means were tested with Student’s t -test and are marked with * when p < 0.05.

    Article Snippet: Anti-ABCB1 (E1Y7B) rabbit mAb (#13342), anti-ABCC1 (D7O8N) rabbit mAb (#14685), ABCG2 (D5V2K) XP ® rabbit mAb (#42078), anti-p300 (D2 × 6N) rabbit mAb (#54062), anti-ABCC4 (D2Q2O) rabbit mAb (#12705), anti-ABCC3 (D8V8J) rabbit mAb (#39909), anti-LSD1 (#2139), anti-histone H3 (#4620; ChIP grade), anti-H3K27ac (#4353), anti-H3K4me3 (#9751), normal rabbit IgG (#2729), anti-histone H3 (1B1B2; for Western blot) mouse mAb (#14269), anti-mouse IgG (H + L), F (ab′)2 fragment (PE conjugate) (#59997), anti-rabbit IgG (H + L), F (ab′)2 fragment (Alexa Fluor ® 488 Conjugate) (#4412), anti-rabbit IgG, HRP-linked antibody (#7074) were from Cell Signaling Technologies (LabJOT, Warsaw, Poland).

    Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction, Western Blot, Confocal Microscopy, Staining

    DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, MRP4, Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Molecular Medicine

    Article Title: The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer

    doi: 10.1186/s10020-020-00174-2

    Figure Lengend Snippet: DDP-resistant ovarian cancer cells possess enhanced metastatic activity and arachidonic acid metabolism. a The procedure for the induction of DDP-resistant SKOV3 cells (upper panel); IC50 and the cell viabilities of SKOV3 and SKOV3-R with different concentrations of DDP treatment (lower panel). b, c The mRNA ( b ) and protein ( c ) levels of MRP1, MRP4, Slug and Snail in SKOV3 and SKOV3-R. d Liver metastasis in nude mice after SKOV3 or SKOV3-R subcutaneous transplantation. e, f . Migration and invasion of SKOV3 and SKOV3-R were tested by wound-healing ( e ) and transwell assays ( f ). g MRM chromatograms of AA, TXB 2 , 12-HETE, 5,6-EET, and 14,15-EET. h The supernatant levels of LTB 4 , 5-HETE, 12-HETE, PGE 2 , TXB 2 , 5,6-EET, 14,15-EET, 14,15-DHET, 11-HETE, and 11,12-DHET in SKOV3 and SKOV3-R. i The heatmap for gene expression of 12LOX , COX1 , CYP2J2 , COX2 , 5LOX , 15LOX , LTA4H and PGDH . j the protein levels of 12LOX and COX1 were assessed by western blotting. Data are expressed as mean ± SEM of 4 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: The primary antibodies include MRP1 [catalog no. 14685S, Cell Signaling Technology, (CST) Danvers, MA, USA], MRP4 (catalog no. 12705, CST), SP1 (catalog no. 9389, CST), 12LOX (catalog no. ab167372, Abcam, Cambridge, UK), Snail (catalog no. 3879, CST), Slug (catalog no. 9585, CST) and β-Actin (catalog no. 3700, CST).

    Techniques: Activity Assay, Transplantation Assay, Migration, Expressing, Western Blot

    Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1 , MRP4 , Snail , Slug , 12LOX , COX1 , and CYP2J2 . b SP1 binding sequences in the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). c ChIP assay of SP1 binding on the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). d, e The mRNA ( d ) and protein ( e ) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in ( d ) are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and * P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P -values of 0.05 or less were considered significant

    Journal: Molecular Medicine

    Article Title: The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer

    doi: 10.1186/s10020-020-00174-2

    Figure Lengend Snippet: Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1 , MRP4 , Snail , Slug , 12LOX , COX1 , and CYP2J2 . b SP1 binding sequences in the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). c ChIP assay of SP1 binding on the promoters of MRP1 ( ABCC1 ), MRP4 ( ABCC4 ), and 12LOX ( ALOX12 ). d, e The mRNA ( d ) and protein ( e ) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in ( d ) are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and * P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P -values of 0.05 or less were considered significant

    Article Snippet: The primary antibodies include MRP1 [catalog no. 14685S, Cell Signaling Technology, (CST) Danvers, MA, USA], MRP4 (catalog no. 12705, CST), SP1 (catalog no. 9389, CST), 12LOX (catalog no. ab167372, Abcam, Cambridge, UK), Snail (catalog no. 3879, CST), Slug (catalog no. 9585, CST) and β-Actin (catalog no. 3700, CST).

    Techniques: Binding Assay, Expressing

    Inhibition of SP1 or 12LOX dampens metastasis and DDP-resistance of SKOV3-R cells in vitro. a-d After incubation with Bai (20 μM) or MA (30 nM) for 24 h, migration and invasion were tested by wound-healing assays ( a ) and transwell assays ( b ); the cell viability percentages were measured by CCK-8 assay ( c, d ). siNC denoted SP1 siRNA negative control, si-SP1 denoted SP1 siRNA. e-g After SKOV3-R cells were treated with these siRNAs, the gene expression levels of SP1 was evaluated by qPCR ( e ) and migration and invasion were tested by wound-healing assays ( f ) and transwell assays ( g ). h-j After administration with Bai (20 μM) or MA (30 nM) for 24 h, the heatmap for gene expression of MRP1 , MRP4 , SP1 , 12LOX , Slug and Snail ( h ) and the protein level of MRP1, MRP4, Slug and Snail were evaluated by western blotting ( i,j ). Data are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001

    Journal: Molecular Medicine

    Article Title: The SP1-12LOX axis promotes chemoresistance and metastasis of ovarian cancer

    doi: 10.1186/s10020-020-00174-2

    Figure Lengend Snippet: Inhibition of SP1 or 12LOX dampens metastasis and DDP-resistance of SKOV3-R cells in vitro. a-d After incubation with Bai (20 μM) or MA (30 nM) for 24 h, migration and invasion were tested by wound-healing assays ( a ) and transwell assays ( b ); the cell viability percentages were measured by CCK-8 assay ( c, d ). siNC denoted SP1 siRNA negative control, si-SP1 denoted SP1 siRNA. e-g After SKOV3-R cells were treated with these siRNAs, the gene expression levels of SP1 was evaluated by qPCR ( e ) and migration and invasion were tested by wound-healing assays ( f ) and transwell assays ( g ). h-j After administration with Bai (20 μM) or MA (30 nM) for 24 h, the heatmap for gene expression of MRP1 , MRP4 , SP1 , 12LOX , Slug and Snail ( h ) and the protein level of MRP1, MRP4, Slug and Snail were evaluated by western blotting ( i,j ). Data are expressed as mean ± SEM of 4 independent experiments. *** P < 0.001

    Article Snippet: The primary antibodies include MRP1 [catalog no. 14685S, Cell Signaling Technology, (CST) Danvers, MA, USA], MRP4 (catalog no. 12705, CST), SP1 (catalog no. 9389, CST), 12LOX (catalog no. ab167372, Abcam, Cambridge, UK), Snail (catalog no. 3879, CST), Slug (catalog no. 9585, CST) and β-Actin (catalog no. 3700, CST).

    Techniques: Inhibition, In Vitro, Incubation, Migration, CCK-8 Assay, Negative Control, Expressing, Western Blot

    Bcl2 and MRP4 are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.

    Journal: Frontiers in Oncology

    Article Title: Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma

    doi: 10.3389/fonc.2019.00027

    Figure Lengend Snippet: Bcl2 and MRP4 are target genes of miR-125a-5p in EC cells . (A) The predicted miR-125a-5p binding site in the 3′UTR of Bcl2 and MRP4 mRNA. Short vertical lines indicated complementary nucleotides. (B) The expression of Bcl2 and MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC group. ** P < 0.01 vs. agomiR-NC group. (C) The relative luciferase reporter assay of HEK 293T cells co-transfected with Bcl2-W or Bcl2-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. Bcl2-W + agomiR-NC group. (D) The relative luciferase reporter assay of HEK 293T cells co-transfected with MRP4-W or MRP4-M and agomiR-125a-5p or agomiR-NC. ** P < 0.01 vs. MRP4-W + agomiR-NC group.

    Article Snippet: PVDF membrane was blocked with Tween-Tris-buffered saline (TTBS), containing 5% non-fat milk at 25°C for 2 h, and hybridized with Bcl2 and MRP4 antibodies (#2872 and #12705; Cell Signaling Technology, Danvers, MA, USA) at 4°C overnight.

    Techniques: Binding Assay, Expressing, Luciferase, Reporter Assay, Transfection

    Enhancement of Bcl2 and MRP4 partially reversed the suppressive effects of up-regulation of miR-125a-5p on paclitaxel resistance in EC cells. (A) The expression of Bcl2 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (B) The expression of MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (C,D) : The IC50 of paclitaxel in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; ## P < 0.01 vs. agomiR-125a-5p + pUC group.

    Journal: Frontiers in Oncology

    Article Title: Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma

    doi: 10.3389/fonc.2019.00027

    Figure Lengend Snippet: Enhancement of Bcl2 and MRP4 partially reversed the suppressive effects of up-regulation of miR-125a-5p on paclitaxel resistance in EC cells. (A) The expression of Bcl2 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (B) The expression of MRP4 protein in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; # P < 0.05 vs. agomiR-125a-5p + pUC group. (C,D) : The IC50 of paclitaxel in Ishikawa/PA and HEC-1A/PA cells. * P < 0.05 vs. agomiR-NC + pUC group; ** P < 0.01 vs. agomiR-NC + pUC group; ## P < 0.01 vs. agomiR-125a-5p + pUC group.

    Article Snippet: PVDF membrane was blocked with Tween-Tris-buffered saline (TTBS), containing 5% non-fat milk at 25°C for 2 h, and hybridized with Bcl2 and MRP4 antibodies (#2872 and #12705; Cell Signaling Technology, Danvers, MA, USA) at 4°C overnight.

    Techniques: Expressing

    ISL pretreatment induced expressions of (A) MRP2, (B) MRP4, and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Journal: Frontiers in Pharmacology

    Article Title: Mechanisms of Triptolide-Induced Hepatotoxicity and Protective Effect of Combined Use of Isoliquiritigenin: Possible Roles of Nrf2 and Hepatic Transporters

    doi: 10.3389/fphar.2018.00226

    Figure Lengend Snippet: ISL pretreatment induced expressions of (A) MRP2, (B) MRP4, and (C) P-gp to protect against TP-induced cytotoxicity. L02 cells were treated with 50 nM TP with or without 2.5, 5, and 7.5 μM ISL pretreatment. The tert -butylhydroquinone (15 μM) was used as an Nrf2 inducer. RT-PCR was used to analyze mRNAs and proteins by Western blot. Data were presented as means ± SE ( n = 3); ∗∗∗ P < 0.001 vs. the control group, ## P < 0.01 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Article Snippet: The antibodies included anti-Nrf2 (sc-722, Santa Cruz), anti-NQO1 (ab28947, Abcam), anti-P-gp (ab170904, Abcam), anti-MRP2 (ab203397, Abcam), anti-MRP4 (#12705, Cell Signaling Technology), anti-GAPDH (#MAB374, Merck Millipore), and anti-Lamin B2 (#12255, Cell Signaling Technology).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effects of TP, ISL, or ISL + TP on the protein levels of (A) MRP2, (B) MRP4, and (C) P-gp and the mRNA levels of (D) Bsep and (E) Oatp2 in mouse livers. Cell lysates were analyzed by Western blot and mRNAs by RT-PCR. Data were presented as means ± SE ( n = 5); ∗ P < 0.05 vs. the control group, ∗∗∗ P < 0.001 vs. the control group, # P < 0.05 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Journal: Frontiers in Pharmacology

    Article Title: Mechanisms of Triptolide-Induced Hepatotoxicity and Protective Effect of Combined Use of Isoliquiritigenin: Possible Roles of Nrf2 and Hepatic Transporters

    doi: 10.3389/fphar.2018.00226

    Figure Lengend Snippet: Effects of TP, ISL, or ISL + TP on the protein levels of (A) MRP2, (B) MRP4, and (C) P-gp and the mRNA levels of (D) Bsep and (E) Oatp2 in mouse livers. Cell lysates were analyzed by Western blot and mRNAs by RT-PCR. Data were presented as means ± SE ( n = 5); ∗ P < 0.05 vs. the control group, ∗∗∗ P < 0.001 vs. the control group, # P < 0.05 vs. the TP group, and ### P < 0.001 vs. the TP group.

    Article Snippet: The antibodies included anti-Nrf2 (sc-722, Santa Cruz), anti-NQO1 (ab28947, Abcam), anti-P-gp (ab170904, Abcam), anti-MRP2 (ab203397, Abcam), anti-MRP4 (#12705, Cell Signaling Technology), anti-GAPDH (#MAB374, Merck Millipore), and anti-Lamin B2 (#12255, Cell Signaling Technology).

    Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction