Journal: Nutrients

Article Title: Antidiabetic Effect of Casein Glycomacropeptide Hydrolysates on High-Fat Diet and STZ-Induced Diabetic Mice via Regulating Insulin Signaling in Skeletal Muscle and Modulating Gut Microbiota

doi: 10.3390/nu12010220

Figure Lengend Snippet: GHP supplementation promotes glycogen synthesis in T2D mice. ( A ) Skeletal muscle glycogen content. ( B ) Protein contents of GSK-3β, p-GSK-3β, p-GS and GS in skeletal muscle and ( C ) quantification data. Protein contents were adjusted to β-tubulin as the loading control. Results were expressed as means ± SEM, n = 8. Different letters (a–c) indicate significant difference ( p < 0.05).

Article Snippet: Blocking the membranes with Tris-buffer saline with 0.1% Tween 20 (TBST) containing 5% skim milk for 1 h, and then incubating membranes with primary antibodies against p-IRS-1 (#2381), IRS-1 (#3407), p-PI3K p85 (#), PI3K p85 (#4257), p-Akt (#4060), Akt (#4691), p-GSK-3β (#9323), GSK-3β (#12356), p-GS (#3891), GS (#3886), GLUT4 (#2213), Na, K-ATPase (#23565), β-tubulin (#2146) (1:1000, Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight.

Techniques: