viability  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc viability
    Viability, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viability/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    viability - by Bioz Stars, 2023-02
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    viability  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc viability
    Viability, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viability/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    viability - by Bioz Stars, 2023-02
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    resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin cell viability kit
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    resazurin cell viability kit - by Bioz Stars, 2023-02
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    resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin cell viability kit
    Inhibitory effects of NAE_2022C on TNFα- and UVB-induced inflammatory responses. ( A ) Brightfield micrographs of C. zofingiensis cultivated at standard growth conditions (stage 1; Upper) and after nitrogen deprivation stress (stage 2; Lower). Scale bar, 10 μm. ( B ) NAE_2022C inhibited TNFα- but not UVB-induced NF-κB responses. HaCaT cells were incubated with 100 nM DM, 750 nM WFA or 10 µg mL −1 NAE_2022C for 1 h and subsequently stimulated with 0.75 ng mL −1 TNFα or irradiated with 0.15 J/cm 2 UVB. The luciferase activity was measured in <t>cell</t> lysates 6 or 24 h post-stimulation, respectively (left y-axis). The cell <t>viability</t> was evaluated using a <t>resazurin</t> assay and shown as orange squares (right y-axis). Data are presented as mean ± SD ( n = 3). ( C ) The suppression of TNFα-induced pro-inflammatory mediators in reconstituted human epidermis (epiCS) by NAE_2022C. The release of cytokines and chemokines was determined in the medium after the treatment of epiCS with 0.75 ng mL −1 TNFα for 24 h (MCP1, RANTES and TNFα) and 48 h (CXCL10) using the Luminex Assay. Data represent the mean ± SD of at least three independent biological replicates. * p < 0.05 vs. samples treated with DMSO and TNFα.
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    resazurin cell viability kit - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "Anti-Inflammatory Extract from Soil Algae Chromochloris zofingiensis Targeting TNFR/NF-κB Signaling at Different Levels"

    Article Title: Anti-Inflammatory Extract from Soil Algae Chromochloris zofingiensis Targeting TNFR/NF-κB Signaling at Different Levels

    Journal: Cells

    doi: 10.3390/cells11091407

    Inhibitory effects of NAE_2022C on TNFα- and UVB-induced inflammatory responses. ( A ) Brightfield micrographs of C. zofingiensis cultivated at standard growth conditions (stage 1; Upper) and after nitrogen deprivation stress (stage 2; Lower). Scale bar, 10 μm. ( B ) NAE_2022C inhibited TNFα- but not UVB-induced NF-κB responses. HaCaT cells were incubated with 100 nM DM, 750 nM WFA or 10 µg mL −1 NAE_2022C for 1 h and subsequently stimulated with 0.75 ng mL −1 TNFα or irradiated with 0.15 J/cm 2 UVB. The luciferase activity was measured in cell lysates 6 or 24 h post-stimulation, respectively (left y-axis). The cell viability was evaluated using a resazurin assay and shown as orange squares (right y-axis). Data are presented as mean ± SD ( n = 3). ( C ) The suppression of TNFα-induced pro-inflammatory mediators in reconstituted human epidermis (epiCS) by NAE_2022C. The release of cytokines and chemokines was determined in the medium after the treatment of epiCS with 0.75 ng mL −1 TNFα for 24 h (MCP1, RANTES and TNFα) and 48 h (CXCL10) using the Luminex Assay. Data represent the mean ± SD of at least three independent biological replicates. * p < 0.05 vs. samples treated with DMSO and TNFα.
    Figure Legend Snippet: Inhibitory effects of NAE_2022C on TNFα- and UVB-induced inflammatory responses. ( A ) Brightfield micrographs of C. zofingiensis cultivated at standard growth conditions (stage 1; Upper) and after nitrogen deprivation stress (stage 2; Lower). Scale bar, 10 μm. ( B ) NAE_2022C inhibited TNFα- but not UVB-induced NF-κB responses. HaCaT cells were incubated with 100 nM DM, 750 nM WFA or 10 µg mL −1 NAE_2022C for 1 h and subsequently stimulated with 0.75 ng mL −1 TNFα or irradiated with 0.15 J/cm 2 UVB. The luciferase activity was measured in cell lysates 6 or 24 h post-stimulation, respectively (left y-axis). The cell viability was evaluated using a resazurin assay and shown as orange squares (right y-axis). Data are presented as mean ± SD ( n = 3). ( C ) The suppression of TNFα-induced pro-inflammatory mediators in reconstituted human epidermis (epiCS) by NAE_2022C. The release of cytokines and chemokines was determined in the medium after the treatment of epiCS with 0.75 ng mL −1 TNFα for 24 h (MCP1, RANTES and TNFα) and 48 h (CXCL10) using the Luminex Assay. Data represent the mean ± SD of at least three independent biological replicates. * p < 0.05 vs. samples treated with DMSO and TNFα.

    Techniques Used: Incubation, Irradiation, Luciferase, Activity Assay, Resazurin Assay, Luminex

    resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin cell viability kit
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    resazurin cell viability kit - by Bioz Stars, 2023-02
    94/100 stars

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    resazurin cell viability kit  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc resazurin cell viability kit
    a Heat maps displaying the relative <t>cell</t> number for each cell line-sgRNA pair 72 h after treatment with 4-Hydroxytamoxifen (4-OHT), Lapatinib, or YM155 as measured using QMAP-Seq. Data are represented as mean of two biologically independent replicates. Asterisks denote positive controls. b Scatterplot of the dose–response curve AUC as determined using QMAP-Seq with one cell line versus QMAP-Seq with multiple cell lines. Common compounds for MDA-MB-231 cells are shown. Statistical significance of Pearson correlation was determined using a two-tailed test ( n = 220 compound-sgRNA combinations). c Volcano plot depicting cell line–gene–drug interactions. Magnitude was determined by calculating the difference in mean AUC between sgRNA and sgNT for every cell line-compound combination. Statistical significance was determined using an unpaired, two-tailed t test ( n = 2 biologically independent replicates). Blue dots indicate interactions where the knockout confers greater sensitivity than sgNT. Red dots indicate interactions where the knockout confers greater resistance than sgNT. d Pathways targeted by compounds involved in the top 60 sensitivity interactions (blue circles) or top 124 resistance interactions (red circles) that were significantly enriched compared to expected pathway representation. Statistical significance of pathway enrichment was determined using a one-tailed binomial test to compare observed distribution with expected distribution ( n = 180 compounds). e List of the top 12 conditions that confer compound sensitivity or resistance. f Top: Dose–response curves for four of the top chemical–genetic interactions as measured using QMAP-Seq. Each data point represents one of two biologically independent replicates. Bottom: Dose–response curves as measured using <t>Resazurin</t> Cell <t>Viability</t> <t>Kit.</t> Each data point represents one of three biologically independent replicates. For clarity, individual proteostasis factor knockout curves are partioned across four panels; sgNT is same in all cases.
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    resazurin cell viability kit - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq"

    Article Title: Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq

    Journal: Nature Communications

    doi: 10.1038/s41467-020-19553-8

    a Heat maps displaying the relative cell number for each cell line-sgRNA pair 72 h after treatment with 4-Hydroxytamoxifen (4-OHT), Lapatinib, or YM155 as measured using QMAP-Seq. Data are represented as mean of two biologically independent replicates. Asterisks denote positive controls. b Scatterplot of the dose–response curve AUC as determined using QMAP-Seq with one cell line versus QMAP-Seq with multiple cell lines. Common compounds for MDA-MB-231 cells are shown. Statistical significance of Pearson correlation was determined using a two-tailed test ( n = 220 compound-sgRNA combinations). c Volcano plot depicting cell line–gene–drug interactions. Magnitude was determined by calculating the difference in mean AUC between sgRNA and sgNT for every cell line-compound combination. Statistical significance was determined using an unpaired, two-tailed t test ( n = 2 biologically independent replicates). Blue dots indicate interactions where the knockout confers greater sensitivity than sgNT. Red dots indicate interactions where the knockout confers greater resistance than sgNT. d Pathways targeted by compounds involved in the top 60 sensitivity interactions (blue circles) or top 124 resistance interactions (red circles) that were significantly enriched compared to expected pathway representation. Statistical significance of pathway enrichment was determined using a one-tailed binomial test to compare observed distribution with expected distribution ( n = 180 compounds). e List of the top 12 conditions that confer compound sensitivity or resistance. f Top: Dose–response curves for four of the top chemical–genetic interactions as measured using QMAP-Seq. Each data point represents one of two biologically independent replicates. Bottom: Dose–response curves as measured using Resazurin Cell Viability Kit. Each data point represents one of three biologically independent replicates. For clarity, individual proteostasis factor knockout curves are partioned across four panels; sgNT is same in all cases.
    Figure Legend Snippet: a Heat maps displaying the relative cell number for each cell line-sgRNA pair 72 h after treatment with 4-Hydroxytamoxifen (4-OHT), Lapatinib, or YM155 as measured using QMAP-Seq. Data are represented as mean of two biologically independent replicates. Asterisks denote positive controls. b Scatterplot of the dose–response curve AUC as determined using QMAP-Seq with one cell line versus QMAP-Seq with multiple cell lines. Common compounds for MDA-MB-231 cells are shown. Statistical significance of Pearson correlation was determined using a two-tailed test ( n = 220 compound-sgRNA combinations). c Volcano plot depicting cell line–gene–drug interactions. Magnitude was determined by calculating the difference in mean AUC between sgRNA and sgNT for every cell line-compound combination. Statistical significance was determined using an unpaired, two-tailed t test ( n = 2 biologically independent replicates). Blue dots indicate interactions where the knockout confers greater sensitivity than sgNT. Red dots indicate interactions where the knockout confers greater resistance than sgNT. d Pathways targeted by compounds involved in the top 60 sensitivity interactions (blue circles) or top 124 resistance interactions (red circles) that were significantly enriched compared to expected pathway representation. Statistical significance of pathway enrichment was determined using a one-tailed binomial test to compare observed distribution with expected distribution ( n = 180 compounds). e List of the top 12 conditions that confer compound sensitivity or resistance. f Top: Dose–response curves for four of the top chemical–genetic interactions as measured using QMAP-Seq. Each data point represents one of two biologically independent replicates. Bottom: Dose–response curves as measured using Resazurin Cell Viability Kit. Each data point represents one of three biologically independent replicates. For clarity, individual proteostasis factor knockout curves are partioned across four panels; sgNT is same in all cases.

    Techniques Used: Two Tailed Test, Knock-Out, One-tailed Test

    resazurin 242 cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin 242 cell viability kit
    Resazurin 242 Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin 242 cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    resazurin 242 cell viability kit - by Bioz Stars, 2023-02
    94/100 stars

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    resazurin solution  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin solution
    Resazurin Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin solution/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin cell viability kit
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resazurin cell viability kit
    Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resazurin cell viability kit/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    fluorometric resazurin cell viability kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc fluorometric resazurin cell viability kit
    Fluorometric Resazurin Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorometric resazurin cell viability kit/product/Cell Signaling Technology Inc
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