Journal: OncoTargets and therapy

Article Title: Paradoxical effects of cellular senescence-inhibited gene involved in hepatocellular carcinoma migration and proliferation by ERK pathway and mesenchymal-like markers

doi: 10.2147/OTT.S188449

Figure Lengend Snippet: Cellular senescence-inhibited gene (CSIG) could promote P-ERK activation and levels of mesenchymal-like markers in SMMC7721 cells. Notes: ( A ) Western blot showed the protein levels of P-ERK in SMMC7721 transfected with control vector (PIRES-flag) and CSIG overexpressed plasmid (PIRES-flag-CSIG). ( B ) P-ERK protein levels in SMMC7721 transfected with negative control and CSIG siRNAs. ( C ) The mRNA levels of CSIG, E-cadherin, Vimentin, N-cadherin, and MMP9 in SMMC7721 cells transfected with CSIG overexpressed plasmid and control vector. ( D ) MMP9 protein levels in SMMC7721 transfected with negative control and CSIG siRNAs. *** P <0.001.

Article Snippet: Anti-phospho-ERK, anti-ERK, anti-MMP9, anti-E-cadherin, anti-Vimentin, anti-N-cadherin, and anti-GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Transfection, Plasmid Preparation, Negative Control

Journal: OncoTargets and therapy

Article Title: Paradoxical effects of cellular senescence-inhibited gene involved in hepatocellular carcinoma migration and proliferation by ERK pathway and mesenchymal-like markers

doi: 10.2147/OTT.S188449

Figure Lengend Snippet: Cellular senescence-inhibited gene (CSIG) could inhibit P-ERK activation and levels of mesenchymal-like markers in Huh7 cells. Notes: ( A ) Western blot showed the protein levels of P-ERK in Huh7 cells transfected with control vector (PIRES-flag) and CSIG overexpressed plasmid (PIRES-flag-CSIG). ( B ) The mRNA levels of CSIG, E-cadherin, Vimentin, N-cadherin, and MMP9 in Huh7 cells transfected with CSIG overexpressed plasmid and control vector. ( C , D ) The protein levels of E-cadherin, Vimentin, N-cadherin, and MMP9 in Huh7 cells transfected with CSIG overexpressed plasmid and control vector. ** P <0.01, *** P <0.001.

Article Snippet: Anti-phospho-ERK, anti-ERK, anti-MMP9, anti-E-cadherin, anti-Vimentin, anti-N-cadherin, and anti-GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Transfection, Plasmid Preparation

Journal: OncoTargets and therapy

Article Title: Paradoxical effects of cellular senescence-inhibited gene involved in hepatocellular carcinoma migration and proliferation by ERK pathway and mesenchymal-like markers

doi: 10.2147/OTT.S188449

Figure Lengend Snippet: Cellular senescence-inhibited gene (CSIG) could promote P-ERK activation and levels of mesenchymal-like markers in SMMC7721 cells. Notes: ( A ) Western blot showed the protein levels of P-ERK in SMMC7721 transfected with control vector (PIRES-flag) and CSIG overexpressed plasmid (PIRES-flag-CSIG). ( B ) P-ERK protein levels in SMMC7721 transfected with negative control and CSIG siRNAs. ( C ) The mRNA levels of CSIG, E-cadherin, Vimentin, N-cadherin, and MMP9 in SMMC7721 cells transfected with CSIG overexpressed plasmid and control vector. ( D ) MMP9 protein levels in SMMC7721 transfected with negative control and CSIG siRNAs. *** P <0.001.

Article Snippet: Anti-phospho-ERK, anti-ERK, anti-MMP9, anti-E-cadherin, anti-Vimentin, anti-N-cadherin, and anti-GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Transfection, Plasmid Preparation, Negative Control

Journal: OncoTargets and therapy

Article Title: Paradoxical effects of cellular senescence-inhibited gene involved in hepatocellular carcinoma migration and proliferation by ERK pathway and mesenchymal-like markers

doi: 10.2147/OTT.S188449

Figure Lengend Snippet: Cellular senescence-inhibited gene (CSIG) could inhibit P-ERK activation and levels of mesenchymal-like markers in Huh7 cells. Notes: ( A ) Western blot showed the protein levels of P-ERK in Huh7 cells transfected with control vector (PIRES-flag) and CSIG overexpressed plasmid (PIRES-flag-CSIG). ( B ) The mRNA levels of CSIG, E-cadherin, Vimentin, N-cadherin, and MMP9 in Huh7 cells transfected with CSIG overexpressed plasmid and control vector. ( C , D ) The protein levels of E-cadherin, Vimentin, N-cadherin, and MMP9 in Huh7 cells transfected with CSIG overexpressed plasmid and control vector. ** P <0.01, *** P <0.001.

Article Snippet: Anti-phospho-ERK, anti-ERK, anti-MMP9, anti-E-cadherin, anti-Vimentin, anti-N-cadherin, and anti-GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Transfection, Plasmid Preparation

Journal: Cancers

Article Title: From Proteomic Mapping to Invasion-Metastasis-Cascade Systemic Biomarkering and Targeted Drugging of Mutant BRAF-Dependent Human Cutaneous Melanomagenesis

doi: 10.3390/cancers13092024

Figure Lengend Snippet: Engagement of hybrid EMT/MET programs during BRAF V600D -dependent melanomagenesis. ( A , B ) Immunofluorescence profiles of epithelial (MET) and mesenchymal (EMT) program protein markers in WM115 (primary) and WM266-4 (metastatic) melanoma cells. ( C ) Western blotting-derived patterns of protein expression being critically implicated in the acquisition of epithelial or mesenchymal (molecular/phenotypic) traits. ( A ) “Vimentin” (M), “N-Cadherin” (M), “E-Cadherin” (E), “ZEB1” (M), “ZEB2” (M), “SLUG” (M) and “p63α” (E). ( B ) “PRRX1” (M), “Keratin-5” (E), “Keratin-8/18” (E/M), “PDGFRβ” (E/M), and “LOXL2” (M). ( C ) “Vimentin” (M), “LOX” (M), “LOXL2” (M), and “Pan-Actin” (protein of reference). E: Epithelial (MET) marker. M: Mesenchymal (EMT) marker. Arrowheads: ( A ) “Vimentin”-positive Invadopodia and ( B ) “PRRX1”-positive globular “specks”; “Keratin-5”-positive Invadopodia. ( A , B ) Scale bars: 2 or 5 μm. ( C ) Molecular weights of identified proteins (major bands) are denoted by numbers at the right side of each respective panel. Protein quantification values (in bar-chart format) are shown in .

Article Snippet: Rabbit monoclonal antibodies raised against (alphabetically ordered) ATF4, ATG7, cleaved (activated) Caspase-3 (Asp175) (a-Caspase-3), DR5, HIF1α, Keratin-5, LC3B, LOX, LOXL2, N-Cadherin, p63α, PDGFRβ, p-GSK3β-Ser 9 (p-GSK3β), p-H2AX-Ser 139 (p-H2AX), p-S6-Ser 235/236 (p-S6), p-SMAD2-Ser 465/467 (p-SMAD2), SLUG, SOX2, SOX9, SOX10, Vimentin, YAP, ZEB1, and ZEB2 were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

Techniques: Immunofluorescence, Western Blot, Derivative Assay, Expressing, Marker

Journal: Cancers

Article Title: From Proteomic Mapping to Invasion-Metastasis-Cascade Systemic Biomarkering and Targeted Drugging of Mutant BRAF-Dependent Human Cutaneous Melanomagenesis

doi: 10.3390/cancers13092024

Figure Lengend Snippet: IMC melanoma signaturing. Immunophenotyping of protein expression in WM115 (primary) and WM266-4 (metastatic) human melanoma cells.

Article Snippet: Rabbit monoclonal antibodies raised against (alphabetically ordered) ATF4, ATG7, cleaved (activated) Caspase-3 (Asp175) (a-Caspase-3), DR5, HIF1α, Keratin-5, LC3B, LOX, LOXL2, N-Cadherin, p63α, PDGFRβ, p-GSK3β-Ser 9 (p-GSK3β), p-H2AX-Ser 139 (p-H2AX), p-S6-Ser 235/236 (p-S6), p-SMAD2-Ser 465/467 (p-SMAD2), SLUG, SOX2, SOX9, SOX10, Vimentin, YAP, ZEB1, and ZEB2 were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

Techniques: Expressing