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tempest dispenser  (Formulatrix)

 
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    Structured Review

    Formulatrix tempest dispenser
    Tempest Dispenser, supplied by Formulatrix, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tv3/pmc11566907-141-60-62
    Average 94 stars, based on 1 article reviews
    tempest dispenser - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Concentration Assay:


    Article Title: Triazolopyridinyl compounds as kinase inhibitors
    Article Snippet: Protein was aliquoted and stored at −80° C. PI3Kδ HTRF Binding Assay A solution was prepared containing 0.2 nM Anti GST-Tb (Cisbio, 61GSTTLB), 40 nM probe and 1 nM GST-tagged PIK3Cδ in complex with PIK3R1 (Invitrogen #PV5273) in FRET Buffer (20 mM HEPES, 10 mM MgCl2, 0.015% Brij-35, 4 mM DTT, 0.05 mg/mL BSA). .. Using Formulatrix Tempest, the detection antibody/enzyme/probe solution (2 mL) was dispensed into wells of a 1536 plate (Black Low Binding Polystyrene 1536 Plate (Corning, 3724)) containing 10 nL of compounds of interest at appropriate concentration in DMSO. ..

    Article Title: 1H-indazole carboxamides as receptor-interacting protein kinase 1 inhibitors (RIPK1)
    Article Snippet: RIPK1 HTRF Binding Assay A solution was prepared containing 0.2 nM Anti GST-Tb (Cisbio, 61GSTTLB), 90.6 nM probe and 1 nM His-GST-TVMV-hRIPK1 (1-324) in FRET Buffer (20 mM HEPES, 10 mM MgCl2, 0.015% Brij-35, 4 mM DTT, 0.05 mg/mL BSA). .. Using Formulatrix Tempest, the detection antibody/enzyme/probe solution (2 mL) was dispensed into wells of a 1536 plate (Black Low Binding Polystyrene 1536 Plate (Corning, 3724)) containing 10 nL of compounds of interest at appropriate concentration in DMSO. ..

    Mutagenesis:


    Staining:

    Article Title: Screening for variable drug responses using human iPSC cohorts
    Article Snippet: .. Cell fixation, permeabilisation, staining and washing steps (2x, wash volume 50 μl) were performed using automated liquid handling systems (405 plate washer, BioTek– Tempest, Formulatrix). .. Drug dosing on 384-well plates was performed using an Echo acoustic liquid handler and corresponding software (Labcyte).

    Article Title: Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa
    Article Snippet: .. A TEMPEST® liquid dispenser (FORMULATRIX) was used to dispense 2μL of the intermediate staining stock solution into each well of the assay plate, to achieve a final dilution of 1μL per 1mL Hoeschst 33342, 0.5μL per 1mL PNA 647, and 0.1μL per 1mL MitoTracker green FM. ..

    Binding Assay:

    Article Title: Triazolopyridinyl compounds as kinase inhibitors
    Article Snippet: Protein was aliquoted and stored at −80° C. PI3Kδ HTRF Binding Assay A solution was prepared containing 0.2 nM Anti GST-Tb (Cisbio, 61GSTTLB), 40 nM probe and 1 nM GST-tagged PIK3Cδ in complex with PIK3R1 (Invitrogen #PV5273) in FRET Buffer (20 mM HEPES, 10 mM MgCl2, 0.015% Brij-35, 4 mM DTT, 0.05 mg/mL BSA). .. Using Formulatrix Tempest, the detection antibody/enzyme/probe solution (2 mL) was dispensed into wells of a 1536 plate (Black Low Binding Polystyrene 1536 Plate (Corning, 3724)) containing 10 nL of compounds of interest at appropriate concentration in DMSO. ..

    Article Title: 1H-indazole carboxamides as receptor-interacting protein kinase 1 inhibitors (RIPK1)
    Article Snippet: RIPK1 HTRF Binding Assay A solution was prepared containing 0.2 nM Anti GST-Tb (Cisbio, 61GSTTLB), 90.6 nM probe and 1 nM His-GST-TVMV-hRIPK1 (1-324) in FRET Buffer (20 mM HEPES, 10 mM MgCl2, 0.015% Brij-35, 4 mM DTT, 0.05 mg/mL BSA). .. Using Formulatrix Tempest, the detection antibody/enzyme/probe solution (2 mL) was dispensed into wells of a 1536 plate (Black Low Binding Polystyrene 1536 Plate (Corning, 3724)) containing 10 nL of compounds of interest at appropriate concentration in DMSO. ..

    Bacteria:

    Article Title: Discovery of an anti-virulence compound that targets the Staphylococcus aureus SaeRS two-component system to inhibit toxic shock syndrome toxin 1 (TSST-1) production
    Article Snippet: .. Briefly, bacteria were grown and the OD 600 was adjusted to 0.01 in fresh media and distributed in 384-wells microplates using the Tempest dispenser (Formulatrix). ..



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    Image Search Results


    Axonal Rtn-1 KD increases outgrowth by promoting Spastin’s microtubule severing. A. Representative fluorescent STED images of distal axons from microfluidic chambers containing control cortical axons or forty-four hours post-axotomy, which were stained for Rtn-1C (green), Spastin (red), and F-actin (phalloidin, blue). Insets show colocalization of Rtn-1C and Spastin. Right: Line scan analysis of Rtn-1C and Spastin highlighted in the white rectangle and their distribution along the axon. Bar graphs show the quantification of colocalization in control and axotomy conditions. Scale bar= 5 µm. Data are displayed as mean ± standard error of 6-7 axons. Unpaired t test; ∗∗p < 0.01 B. Representative images of axonal fields from microfluidic chambers 48 hours post-axotomy under different treatments: control sequence (Scrambled, Scr), Rtn-1 knockdown (Rtn-1 siRNA), and Spastin pharmacological inhibition (SPZT), and labeled with β3-tubulin (β3-tub). Scale bar represents 200 µm. C. Quantification of axon outgrowth under the treatments shown in A. The Area under the curve (AUC) was measured as the sum of the relative number of intersections at different distance ranges from the groove in the microfluidic chambers (0-500 µm; 500-1000 µm and 1000-1500 µm). Scale bar represents 10 µm. Data are displayed as mean ± standard error of 22-24 axonal fields for condition from N=3 biological replicates. One-way ANOVA followed by Tukey’s posthoc test; ∗p < 0.05; ∗∗p < 0.01. D. Fluorescence intensity profiles of β3-tubulin, Rtn-1C, and Spastin in axons and quantifications of intensity changes of indicated proteins as the fluorescence AUC fold change of the last 50 µm compared to Scr. Data are displayed as mean ± standard error of 24-40 axons for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗∗∗p < 0.001 E. Axonal kymographs showing dynamic microtubule behavior in neurons with indicated treatments after axotomy, dashes represent the traces of EB3-GFP (end-binding protein 3). Scale bars represent 10 µm and 30 seconds, respectively. F. Analysis of microtubule dynamics parameters in non-injured and injured axons with different treatments: growth rate, track length, and lifetime of microtubule plus-end tracks. Data are displayed as mean ± standard error of 146-377 comets for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.001.

    Journal: bioRxiv

    Article Title: Local Synthesis of Reticulon-1C Lessens the Outgrowth of Injured Axons by Controlling Spastin Activity

    doi: 10.1101/2024.08.11.607514

    Figure Lengend Snippet: Axonal Rtn-1 KD increases outgrowth by promoting Spastin’s microtubule severing. A. Representative fluorescent STED images of distal axons from microfluidic chambers containing control cortical axons or forty-four hours post-axotomy, which were stained for Rtn-1C (green), Spastin (red), and F-actin (phalloidin, blue). Insets show colocalization of Rtn-1C and Spastin. Right: Line scan analysis of Rtn-1C and Spastin highlighted in the white rectangle and their distribution along the axon. Bar graphs show the quantification of colocalization in control and axotomy conditions. Scale bar= 5 µm. Data are displayed as mean ± standard error of 6-7 axons. Unpaired t test; ∗∗p < 0.01 B. Representative images of axonal fields from microfluidic chambers 48 hours post-axotomy under different treatments: control sequence (Scrambled, Scr), Rtn-1 knockdown (Rtn-1 siRNA), and Spastin pharmacological inhibition (SPZT), and labeled with β3-tubulin (β3-tub). Scale bar represents 200 µm. C. Quantification of axon outgrowth under the treatments shown in A. The Area under the curve (AUC) was measured as the sum of the relative number of intersections at different distance ranges from the groove in the microfluidic chambers (0-500 µm; 500-1000 µm and 1000-1500 µm). Scale bar represents 10 µm. Data are displayed as mean ± standard error of 22-24 axonal fields for condition from N=3 biological replicates. One-way ANOVA followed by Tukey’s posthoc test; ∗p < 0.05; ∗∗p < 0.01. D. Fluorescence intensity profiles of β3-tubulin, Rtn-1C, and Spastin in axons and quantifications of intensity changes of indicated proteins as the fluorescence AUC fold change of the last 50 µm compared to Scr. Data are displayed as mean ± standard error of 24-40 axons for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗∗∗p < 0.001 E. Axonal kymographs showing dynamic microtubule behavior in neurons with indicated treatments after axotomy, dashes represent the traces of EB3-GFP (end-binding protein 3). Scale bars represent 10 µm and 30 seconds, respectively. F. Analysis of microtubule dynamics parameters in non-injured and injured axons with different treatments: growth rate, track length, and lifetime of microtubule plus-end tracks. Data are displayed as mean ± standard error of 146-377 comets for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.001.

    Article Snippet: A 63x objective and GFP single band exciters ET filter set (excitation 470/40, emission 59022m, dichroic 59022BS) were used. mEmerald-EB3-C-20 was a gift from Michael Davidson (Addgene plasmid # 54076; http://n2t.net/addgene:54076 ; RRID:Addgene_54076)

    Techniques: Control, Staining, Sequencing, Knockdown, Inhibition, Labeling, Fluorescence, Binding Assay