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tempest liquid dispenser system  (Formulatrix)

 
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    Formulatrix tempest liquid dispenser system
    Tempest Liquid Dispenser System, supplied by Formulatrix, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tv3/TEMPEST/pmc11575580-248-1-5
    Average 94 stars, based on 1 article reviews
    tempest liquid dispenser system - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: High-throughput screening reveals diverse lambdoid prophage inducers
    Article Snippet: Subsequently, 30 μl of culture was added using the Tempest Liquid Dispenser (Formulatrix, Dubai, UAE).

    Article Title: Synthesis of 3'-modified xylofuranosyl nucleosides bearing 5'-silyl or -butyryl groups and their antiviral effect against RNA viruses.
    Article Snippet: Tempest liquid dispenser system (Formulatrix) was used for all dispensing steps.

    Article Title: Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform.
    Article Snippet: The customer supplied component consists of various peripheral instruments, including Brooks Automation X-peel desealer, Biotek EL406 series washer/dispenser/shaker, Agilent PlateLoc sealer, BioNEX HIG 5000 centrifuge, Biotek MultifloFX multimode dispenser, BMG Labtech Pherastar (plate reader), Cytomat incubator, and Formulatrix Tempest (multichannel dispenser).

    Article Title: Amides of moronic acid and morolic acid with the tripeptides MAG and GAM targeting antimicrobial, antiviral and cytotoxic effects
    Article Snippet: The Tempest liquid dispenser system (Formulatrix) was used for all dispensing steps.

    Incubation:

    Article Title: Synthesis, H 2 S releasing properties, antiviral and antioxidant activities and acute cardiac effects of nucleoside 5'-dithioacetates.
    Article Snippet: .. Following two days incubation at 37 °C in 5% CO2 the cell viability was determined by adding CellTiter-Glo luminescent cell reagent (Promega) using Tempest liquid dispenser (Formulatrix). .. The luminescent signal produced by ATP-dependent oxidation of luciferin by viable cells was measured using EnVision plate reader (Perkin Elmer).

    Article Title: Synthesis, H2S releasing properties, antiviral and antioxidant activities and acute cardiac effects of nucleoside 5’-dithioacetates
    Article Snippet: .. Following two days incubation at 37°C in 5% CO2 the cell viability was determined by adding CellTiter-Glo luminescent cell reagent (Promega) using Tempest liquid dispenser (Formulatrix). .. The luminescent signal produced by ATPdependent oxidation of luciferin by viable cells was measured using EnVision plate reader (Perkin Elmer).

    Article Title: Synthesis, H 2 S releasing properties, antiviral and antioxidant activities and acute cardiac effects of nucleoside 5′-dithioacetates
    Article Snippet: .. Following two days incubation at 37 °C in 5% CO 2 the cell viability was determined by adding CellTiter-Glo luminescent cell reagent (Promega) using Tempest liquid dispenser (Formulatrix). .. The luminescent signal produced by ATP-dependent oxidation of luciferin by viable cells was measured using EnVision plate reader (Perkin Elmer).

    Activity Assay:

    Article Title: Amides of moronic acid and morolic acid with the tripeptides MAG and GAM targeting antimicrobial, antiviral and cytotoxic effects.
    Article Snippet: .. The anti-HIV-1 and anti-HSV-1 activity and cytotoxicity in the MT-4 cells or Vero cells, respectively, were determined as recently described.18 The Tempest liquid dispenser system (Formulatrix) was used for all dispensing steps. .. The cytotoxicity screening tests were performed according to the standard experimental procedure published earlier.15 Cell viability was measured in CCRF-CEM, human T-lymphoblastic leukemia; MCF7, human breast adenocarcinoma; HeLa, human cervical cancer; G-361, malignant melanoma or BJ, normal human foreskin fibroblasts after 72 h using resazurin (Sigma-Aldrich).



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    Image Search Results


    Axonal Rtn-1 KD increases outgrowth by promoting Spastin’s microtubule severing. A. Representative fluorescent STED images of distal axons from microfluidic chambers containing control cortical axons or forty-four hours post-axotomy, which were stained for Rtn-1C (green), Spastin (red), and F-actin (phalloidin, blue). Insets show colocalization of Rtn-1C and Spastin. Right: Line scan analysis of Rtn-1C and Spastin highlighted in the white rectangle and their distribution along the axon. Bar graphs show the quantification of colocalization in control and axotomy conditions. Scale bar= 5 µm. Data are displayed as mean ± standard error of 6-7 axons. Unpaired t test; ∗∗p < 0.01 B. Representative images of axonal fields from microfluidic chambers 48 hours post-axotomy under different treatments: control sequence (Scrambled, Scr), Rtn-1 knockdown (Rtn-1 siRNA), and Spastin pharmacological inhibition (SPZT), and labeled with β3-tubulin (β3-tub). Scale bar represents 200 µm. C. Quantification of axon outgrowth under the treatments shown in A. The Area under the curve (AUC) was measured as the sum of the relative number of intersections at different distance ranges from the groove in the microfluidic chambers (0-500 µm; 500-1000 µm and 1000-1500 µm). Scale bar represents 10 µm. Data are displayed as mean ± standard error of 22-24 axonal fields for condition from N=3 biological replicates. One-way ANOVA followed by Tukey’s posthoc test; ∗p < 0.05; ∗∗p < 0.01. D. Fluorescence intensity profiles of β3-tubulin, Rtn-1C, and Spastin in axons and quantifications of intensity changes of indicated proteins as the fluorescence AUC fold change of the last 50 µm compared to Scr. Data are displayed as mean ± standard error of 24-40 axons for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗∗∗p < 0.001 E. Axonal kymographs showing dynamic microtubule behavior in neurons with indicated treatments after axotomy, dashes represent the traces of EB3-GFP (end-binding protein 3). Scale bars represent 10 µm and 30 seconds, respectively. F. Analysis of microtubule dynamics parameters in non-injured and injured axons with different treatments: growth rate, track length, and lifetime of microtubule plus-end tracks. Data are displayed as mean ± standard error of 146-377 comets for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.001.

    Journal: bioRxiv

    Article Title: Local Synthesis of Reticulon-1C Lessens the Outgrowth of Injured Axons by Controlling Spastin Activity

    doi: 10.1101/2024.08.11.607514

    Figure Lengend Snippet: Axonal Rtn-1 KD increases outgrowth by promoting Spastin’s microtubule severing. A. Representative fluorescent STED images of distal axons from microfluidic chambers containing control cortical axons or forty-four hours post-axotomy, which were stained for Rtn-1C (green), Spastin (red), and F-actin (phalloidin, blue). Insets show colocalization of Rtn-1C and Spastin. Right: Line scan analysis of Rtn-1C and Spastin highlighted in the white rectangle and their distribution along the axon. Bar graphs show the quantification of colocalization in control and axotomy conditions. Scale bar= 5 µm. Data are displayed as mean ± standard error of 6-7 axons. Unpaired t test; ∗∗p < 0.01 B. Representative images of axonal fields from microfluidic chambers 48 hours post-axotomy under different treatments: control sequence (Scrambled, Scr), Rtn-1 knockdown (Rtn-1 siRNA), and Spastin pharmacological inhibition (SPZT), and labeled with β3-tubulin (β3-tub). Scale bar represents 200 µm. C. Quantification of axon outgrowth under the treatments shown in A. The Area under the curve (AUC) was measured as the sum of the relative number of intersections at different distance ranges from the groove in the microfluidic chambers (0-500 µm; 500-1000 µm and 1000-1500 µm). Scale bar represents 10 µm. Data are displayed as mean ± standard error of 22-24 axonal fields for condition from N=3 biological replicates. One-way ANOVA followed by Tukey’s posthoc test; ∗p < 0.05; ∗∗p < 0.01. D. Fluorescence intensity profiles of β3-tubulin, Rtn-1C, and Spastin in axons and quantifications of intensity changes of indicated proteins as the fluorescence AUC fold change of the last 50 µm compared to Scr. Data are displayed as mean ± standard error of 24-40 axons for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗∗∗p < 0.001 E. Axonal kymographs showing dynamic microtubule behavior in neurons with indicated treatments after axotomy, dashes represent the traces of EB3-GFP (end-binding protein 3). Scale bars represent 10 µm and 30 seconds, respectively. F. Analysis of microtubule dynamics parameters in non-injured and injured axons with different treatments: growth rate, track length, and lifetime of microtubule plus-end tracks. Data are displayed as mean ± standard error of 146-377 comets for condition from N=3 biological replicates. Kruskal-Wallis followed by Dunn’s posthoc test; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.001.

    Article Snippet: A 63x objective and GFP single band exciters ET filter set (excitation 470/40, emission 59022m, dichroic 59022BS) were used. mEmerald-EB3-C-20 was a gift from Michael Davidson (Addgene plasmid # 54076; http://n2t.net/addgene:54076 ; RRID:Addgene_54076)

    Techniques: Control, Staining, Sequencing, Knockdown, Inhibition, Labeling, Fluorescence, Binding Assay