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Promega superscriptii reverse transcriptase
Superscriptii Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscriptiii+transcriptase/superscriptii+reverse+transcriptase/pmc03822633-98-28-31
Average 90 stars, based on 1 article reviews
superscriptii reverse transcriptase - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Construct:

Article Title: Soluble T-cell receptor design influences functional yield in an E . coli chaperone-assisted expression system
Article Snippet: .. The ct-dsTCR construct [ ] was generated from mRNA previously isolated from TCC380.E48 using random hexamer primers (Promega) and SuperScriptII reverse transcriptase. ..

Generated:

Article Title: Soluble T-cell receptor design influences functional yield in an E . coli chaperone-assisted expression system
Article Snippet: .. The ct-dsTCR construct [ ] was generated from mRNA previously isolated from TCC380.E48 using random hexamer primers (Promega) and SuperScriptII reverse transcriptase. ..

Isolation:

Article Title: Soluble T-cell receptor design influences functional yield in an E . coli chaperone-assisted expression system
Article Snippet: .. The ct-dsTCR construct [ ] was generated from mRNA previously isolated from TCC380.E48 using random hexamer primers (Promega) and SuperScriptII reverse transcriptase. ..

Article Title: The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells
Article Snippet: .. Total RNA was isolated from sub-confluent cell cultures and cell lines using Qiazol reagent (Qiagen, Hilden, Germany) and purified via RNeasy columns (Qiagen). cDNA synthesis was performed with SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo-dT primers as described [ ]. .. High molecular weight genomic DNA from cell lines was isolated using the blood and cell culture DNA kit (Qiagen) with additional proteinase K treatment.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. RNA extraction Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Reverse transcription–polymerase chain reaction (RT‐PCR) Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Random Hexamer:

Article Title: Soluble T-cell receptor design influences functional yield in an E . coli chaperone-assisted expression system
Article Snippet: .. The ct-dsTCR construct [ ] was generated from mRNA previously isolated from TCC380.E48 using random hexamer primers (Promega) and SuperScriptII reverse transcriptase. ..

Reverse Transcription:

Article Title: Soluble T-cell receptor design influences functional yield in an E . coli chaperone-assisted expression system
Article Snippet: .. The ct-dsTCR construct [ ] was generated from mRNA previously isolated from TCC380.E48 using random hexamer primers (Promega) and SuperScriptII reverse transcriptase. ..

Article Title: MTDH/AEG-1 contributes to central features of the neoplastic phenotype in bladder cancer.
Article Snippet: Objectives: Carcinoma of the bladder is the fifth most common cancer whose incidence continues to rise.. MTDH/AEG-1 is associated with the initiation and progression of many cancers including breast, hepatocellular, ovarian, and colorectal carcinomas.. However, the expression and functional importance of MTDH/AEG-1 in bladder cancer remains unknown.

Article Title: The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells
Article Snippet: .. Total RNA was isolated from sub-confluent cell cultures and cell lines using Qiazol reagent (Qiagen, Hilden, Germany) and purified via RNeasy columns (Qiagen). cDNA synthesis was performed with SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo-dT primers as described [ ]. .. High molecular weight genomic DNA from cell lines was isolated using the blood and cell culture DNA kit (Qiagen) with additional proteinase K treatment.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Article Title: Anticancer and anti-angiogenic activities of extract from Actinidia eriantha Benth root.
Article Snippet: Ethnopharmacological relevance: The roots of Actinidia eriantha Benth (AER) are commonly used traditional folk medicine for the treatment of gastric carcinoma, nasopharyngeal carcinoma, and breast carcinoma.. Besides, the anti-proliferative and immunomodulatory effects of AER polysaccharides on tumor-bearing mice have been reported previously.. Aim of the study: This work was carried out to investigate the anticancer and anti-angiogenic activities of AER.

Article Title: Absence of PIWIL2 (HILI) expression in human bladder cancer cell lines and tissues.
Article Snippet: Background: PIWIL2, a member of Argonaute family of proteins, is exclusively expressed in testis and functions in development and maintenance of germline stem cells.. Recently, ectopic expression of PIWIL2 has been reported in a variety of human and mouse tumors.. To investigate a potential involvement of PIWIL2 in human bladder cancer, we examined its expression in several human bladder cancer cell lines, normal uroepithelial cell cultures, and some bladder tissues.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. RNA extraction Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Reverse transcription–polymerase chain reaction (RT‐PCR) Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Article Title: GSK3β regulates Bcl2L12 and Bcl2L12A anti-apoptosis signaling in glioblastoma and is inhibited by LiCl.
Article Snippet: Total RNA was prepared from tissue samples and cultured cells using the acidic guanidine isothiosulfate phenol-chloroform technique followed by DNase I treatment (Promega). .. Total RNA (2 μg) from each sample was reverse transcribed in a total volume of 22 μl using SuperscriptII reverse transcriptase (Promega) according to the manufacturer’s protocol. ..

DNA Synthesis:

Article Title: MTDH/AEG-1 contributes to central features of the neoplastic phenotype in bladder cancer.
Article Snippet: Objectives: Carcinoma of the bladder is the fifth most common cancer whose incidence continues to rise.. MTDH/AEG-1 is associated with the initiation and progression of many cancers including breast, hepatocellular, ovarian, and colorectal carcinomas.. However, the expression and functional importance of MTDH/AEG-1 in bladder cancer remains unknown.

Purification:

Article Title: The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells
Article Snippet: .. Total RNA was isolated from sub-confluent cell cultures and cell lines using Qiazol reagent (Qiagen, Hilden, Germany) and purified via RNeasy columns (Qiagen). cDNA synthesis was performed with SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo-dT primers as described [ ]. .. High molecular weight genomic DNA from cell lines was isolated using the blood and cell culture DNA kit (Qiagen) with additional proteinase K treatment.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. RNA extraction Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Reverse transcription–polymerase chain reaction (RT‐PCR) Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

cDNA Synthesis:

Article Title: The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells
Article Snippet: .. Total RNA was isolated from sub-confluent cell cultures and cell lines using Qiazol reagent (Qiagen, Hilden, Germany) and purified via RNeasy columns (Qiagen). cDNA synthesis was performed with SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo-dT primers as described [ ]. .. High molecular weight genomic DNA from cell lines was isolated using the blood and cell culture DNA kit (Qiagen) with additional proteinase K treatment.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Article Title: Absence of PIWIL2 (HILI) expression in human bladder cancer cell lines and tissues.
Article Snippet: Background: PIWIL2, a member of Argonaute family of proteins, is exclusively expressed in testis and functions in development and maintenance of germline stem cells.. Recently, ectopic expression of PIWIL2 has been reported in a variety of human and mouse tumors.. To investigate a potential involvement of PIWIL2 in human bladder cancer, we examined its expression in several human bladder cancer cell lines, normal uroepithelial cell cultures, and some bladder tissues.

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. RNA extraction Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Reverse transcription–polymerase chain reaction (RT‐PCR) Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).

Extraction:

Article Title: Absence of PIWIL2 (HILI) expression in human bladder cancer cell lines and tissues.
Article Snippet: Background: PIWIL2, a member of Argonaute family of proteins, is exclusively expressed in testis and functions in development and maintenance of germline stem cells.. Recently, ectopic expression of PIWIL2 has been reported in a variety of human and mouse tumors.. To investigate a potential involvement of PIWIL2 in human bladder cancer, we examined its expression in several human bladder cancer cell lines, normal uroepithelial cell cultures, and some bladder tissues.

RNA Extraction:

Article Title: Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710
Article Snippet: .. RNA extraction Total RNA was isolated using Qiazol reagent and purified using RNeasy columns (both from Qiagen, Hilden, Germany). cDNA synthesis was performed using SuperScriptII reverse transcriptase (Promega, Mannheim, Germany) with oligo‐dT primers, as described elsewhere ( 24 ). .. Reverse transcription–polymerase chain reaction (RT‐PCR) Real‐time RT‐PCR assays were performed using the LightCycler II apparatus (Roche, Mannheim, Germany).



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