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stc  (Bio-Rad)


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    Structured Review

    Bio-Rad stc
    Stc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stc-751/Liquichek+Spinal+Fluid+Control/pm25176419-49-5-67
    Average 92 stars, based on 7 article reviews
    stc - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Digital PCR:

    Article Title: An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue
    Article Snippet: Please cite this article as: Boxman, I.L.A., Molin, R., Persson, S., Juréus, A., Jansen, C.C.C., Sosef, N.P., Le Guyader, S.F., Ollivier, J., Summa, M., Hautaniemi, M., Suffredini, E., Di Pasquale, S., Myrmel, M., Khatri, M., Jamnikar-Ciglenecki, U., Kusar, D., Moor, D., Butticaz, L., Lowther, J.A., Walker, D.I., Stapleton, T., Simonsson, M., Dirks, René.A.M., An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue, Food Microbiology (2024), doi: https://doi.org/10.1016/j.fm.2024.104478.

    Blocking Assay:

    Article Title: Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants?
    Article Snippet: The proteins in the gels were either visualized by silver staining (ProteoSilver; Sigma-Aldrich) or transferred from the gel to a nitrocellulose membrane (Bio-Rad) using a Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad). .. After blocking D ow nloaded from https://academ ic.oup.com /jxb/article/75/1/334/7273981 by guest on 16 January 2024 in TBS-T containing 5% BSA overnight, the membranes were incubated with the primary antibody for 1 h at room temperature, and after washing, the membrane was incubated with the secondary antibody: goat antirabbit IgG (H + L) horseradish peroxidase conjugate (Bio-Rad). .. Blots were visualized and chemiluminescence was quantified by an Amersham Imager 600 gel scanner (GE Healthcare Life Sciences, Tokyo, Japan).

    Incubation:

    Article Title: Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants?
    Article Snippet: The proteins in the gels were either visualized by silver staining (ProteoSilver; Sigma-Aldrich) or transferred from the gel to a nitrocellulose membrane (Bio-Rad) using a Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad). .. After blocking D ow nloaded from https://academ ic.oup.com /jxb/article/75/1/334/7273981 by guest on 16 January 2024 in TBS-T containing 5% BSA overnight, the membranes were incubated with the primary antibody for 1 h at room temperature, and after washing, the membrane was incubated with the secondary antibody: goat antirabbit IgG (H + L) horseradish peroxidase conjugate (Bio-Rad). .. Blots were visualized and chemiluminescence was quantified by an Amersham Imager 600 gel scanner (GE Healthcare Life Sciences, Tokyo, Japan).

    Membrane:

    Article Title: Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants?
    Article Snippet: The proteins in the gels were either visualized by silver staining (ProteoSilver; Sigma-Aldrich) or transferred from the gel to a nitrocellulose membrane (Bio-Rad) using a Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad). .. After blocking D ow nloaded from https://academ ic.oup.com /jxb/article/75/1/334/7273981 by guest on 16 January 2024 in TBS-T containing 5% BSA overnight, the membranes were incubated with the primary antibody for 1 h at room temperature, and after washing, the membrane was incubated with the secondary antibody: goat antirabbit IgG (H + L) horseradish peroxidase conjugate (Bio-Rad). .. Blots were visualized and chemiluminescence was quantified by an Amersham Imager 600 gel scanner (GE Healthcare Life Sciences, Tokyo, Japan).

    Article Title: Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats.
    Article Snippet: .. Proteins were transferred onto a nitrocellulose blotting membrane (Bio-Rad, Hercules, CA, USA) by immersing cassettes in a tank filled with blotting/transfer buffer (3.03 g of trizma base, 14.4 g of glycine, 0.375 g of SDS, 200 mL of MeOH, and 800 mL of dH2O) and left overnight at 25 V. The blots were washed for 5 min with TBST (6.6 g of NaCl, 25 mL of 1 M Tris HCl pH 7.5, and 0.05% Tween 20 added to 1L of dH2O). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Long COVID neuropsychological follow-up: Is cognitive rehabilitation relevant?
    Article Snippet: .. 2x qPCRBIO SyGreen Mix Lo-ROX (PCRBIOSYSTEMS, UK) was used for qPCR 752 analysis. qPCR analyses were performed using a CFX96 or CFX384 connect real-time PCR 753 detection system (Bio-Rad). .. 754 IAA measurements 755 Seven-day old seedlings pre-grown on a mesh strip on agar plates containing half-756 strength MS medium under a 16-h light/8-h dark photoperiod at 22°C were transferred to 757 fresh half-strength MS agar plates alone or containing 125 mM NaCl (Duchefa).

    Nucleic Acid Electrophoresis:

    Article Title: Cellular Pre-Adaptation to the High O 2 Concentration Used in Standard Cell Culture Confers Resistance to Subsequent H 2 O 2 -Induced Cell Death
    Article Snippet: .. The proteins were separated by gel electrophoresis at 100 V for 90 min using pre-cast 8–16% polyacrylamide gels (BioRad, Watford, UK) in sodium dodecyl sulphate (SDS) buffer (30.3 g Tris base, 144.2 g glycine, 10 g SDS made up to 1 L in dH 2 O). .. Protein transfer was performed utilising a nitrocellulose TransBlot Turbo transfer system kit (BioRad).

    Bioprocessing:

    Article Title: Method of treating solid cancers with bispecific interleukin-anti-TCRß molecules
    Article Snippet: .. TABLE 13 Exemplary anti-TCRβV antibody molecules TRBV name TRBV Reagents monoclonal antibodies gene allele name Clone name and Specificity Company product Isotype TRBV2 TRBV2*01 IsMMU 546 (TRBV2) Serotec V BETA 22 Mouse TRBV2*02 Coulter Vbeta22 IgG1 TRBV2*03 TRBV3-1 TRBV3-1*01 FIN9 (TRBV3-1) Serotec Vbeta9 Mouse Coulter Vbeta9 IgG2a TRBV3-1*02 AMKB1-2 (TRBV3-1) BD Biosciences Mouse Vbeta9 IgG1 TRBV4-1 TRBV4-1*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-1*02 3G5 (TRBV4-1) Pierce Endogen V beta Mouse 7.1 IgG2b TRBV4-2 TRBV4-2*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse TRBV4-2*02 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-3 TRBV4-3*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse TRBV4-3*02 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-3*03 TRBV4-3*04 ZIZOU4 (TRBV4-3) Coulter Vbeta7.2 Mouse IgG2a TRBV5-1 TRBV5-1*01 IMMU157 (TRBV5-1) Serotec Vbeta5.1 Mouse Coulter Vbeta5.1 IgG2a TRBV5-1*02 LC4 (TRBV5-1) Pierce Endogen V beta Mouse 5(c) IgG1 BD Biosciences Vbeta5(c) TRBV5-4 TRBV5-4*01 TRBV5-4*02 TRBV5-4*03 TRBV5-4*04 TRBV5-5 TRBV5-5*01 3D11 (TRBV5-5) Serotec VBETA5.3 Mouse Coulter Vbeta5.3 IgG1 TRBV5-5*02 1C1 (TRBV5-5, TRBV5-6) Pierce Endogen V beta Mouse 5(a) IgG1 BD Biosciences Vbeta5(a) TRBV5-5*03 W112 (TRBV5-5) Pierce Endogen V beta Mouse 5(b) IgG1 Serotec V beta 5.2/5.3 MH3-2 (TRBV5-5, BD Biosciences TRBV5-6) Vbeta5(b) BD Biosciences Mouse Vbeta5 IgG2a 4H11 (TM27) as disclosed in U.S. Pat. ..

    Article Title: Anti-TCR antibody molecules and uses thereof
    Article Snippet: .. TABLE 13 Exemplary anti-TCRBV antibody molecules TRBV name TRBV Reagents monoclonal antibodies gene allele name Clone name and Specificity Company product Isotype TRBV2 TRBV2*01 IsMMU 546 (TRBV2) Serotec V BETA 22 Mouse TRBV2*02 Coulter Vbeta22 IgG1 TRBV2*03 TRBV3-1 TRBV3-1*01 FIN9 (TRBV3-1) Serotec Vbeta9 Mouse Coulter Vbeta9 IgG2a TRBV3-1*02 AMKB1-2 (TRBV3-1) BD Biosciences Mouse Vbeta9 IgG1 TRBV4-1 TRBV4-1*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-1*02 3G5 (TRBV4-1) Pierce Endogen V beta Mouse 7.1 IgG2b TRBV4-2 TRBV4-2*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse TRBV4-2*02 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-3 TRBV4-3*01 ZOE (TRBV4-1, TRBV4- Serotec V BETA 7 Mouse TRBV4-3*02 2, TRBV4-3) Coulter Vbeta7 IgG2a TRBV4-3*03 TRBV4-3*04 ZIZOU4 (TRBV4-3) Coulter Vbeta7.2 Mouse IgG2a TRBV5-1 TRBV5-1*01 IMMU157 (TRBV5-1) Serotec Vbeta5.1 Mouse Coulter Vbeta5.1 IgG2a TRBV5-1*02 LC4 (TRBV5-1) Pierce Endogen V beta Mouse 5(c) IgG1 BD Biosciences Vbeta5(c) TRBV5-4 TRBV5-4*01 TRBV5-4*02 TRBV5-4*03 TRBV5-4*04 TRBV5-5 TRBV5-5*01 3D11 (TRBV5-5) Serotec VBETA5.3 Mouse Coulter Vbeta5.3 IgG1 TRBV5-5*02 1C1 (TRBV5-5, TRBV5-6) Pierce Endogen V beta Mouse 5(a) IgG1 BD Biosciences Vbeta5(a) TRBV5-5*03 W112 (TRBV5-5) Pierce Endogen V beta Mouse 5(b) IgG1 Serotec V beta 5.2/5.3 MH3-2 (TRBV5-5, BD Biosciences TRBV5-6) Vbeta5(b) BD Biosciences Mouse Vbeta5 IgG2a 4H11 (TM27) as disclosed in U.S. Pat. ..

    Quantitative RT-PCR:

    Article Title: HIF1α-glycolysis engages activation-induced cell death to drive IFN-γ induction in hypoxic T cells
    Article Snippet: .. First-strand cDNA was 752 diluted 20 times for quantitative RT-PCR which was performed on Bio-Rad CFX96 instrument. ..



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    Comparisons of effects of GV-58, GV-58 plus <t>ω-conotoxin</t> <t>MVIID,</t> and GV-58 plus tetrodotoxin (TTX) on the peak amplitude of I Na recorded from GH 3 cells. Cells were immersed in Ca 2+ -free Tyrode’s solution containing 10 mM TEA, and the pipettes used were filled with Cs + -containing solution. Current amplitude was taken at the beginning of the brief depolarizing pulse from −80 to −10 mV. Each bar indicates the mean ± SEM (n = 8 for each bar). Of notice, the peak I Na in GH 3 cells is subject to inhibition by TTX but not by ω-conotoxin MVIID. * Significantly different from control ( p < 0.05) and ** Significantly different from GV-58 (3 μM) alone group ( p < 0.05). Statistical analyses were made by one-way ANOVA among different groups ( p < 0.05).
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    Image Search Results


    Comparisons of effects of GV-58, GV-58 plus ω-conotoxin MVIID, and GV-58 plus tetrodotoxin (TTX) on the peak amplitude of I Na recorded from GH 3 cells. Cells were immersed in Ca 2+ -free Tyrode’s solution containing 10 mM TEA, and the pipettes used were filled with Cs + -containing solution. Current amplitude was taken at the beginning of the brief depolarizing pulse from −80 to −10 mV. Each bar indicates the mean ± SEM (n = 8 for each bar). Of notice, the peak I Na in GH 3 cells is subject to inhibition by TTX but not by ω-conotoxin MVIID. * Significantly different from control ( p < 0.05) and ** Significantly different from GV-58 (3 μM) alone group ( p < 0.05). Statistical analyses were made by one-way ANOVA among different groups ( p < 0.05).

    Journal: Biomedicines

    Article Title: Activation of Voltage-Gated Na + Current by GV-58, a Known Activator of Ca V Channels

    doi: 10.3390/biomedicines10030721

    Figure Lengend Snippet: Comparisons of effects of GV-58, GV-58 plus ω-conotoxin MVIID, and GV-58 plus tetrodotoxin (TTX) on the peak amplitude of I Na recorded from GH 3 cells. Cells were immersed in Ca 2+ -free Tyrode’s solution containing 10 mM TEA, and the pipettes used were filled with Cs + -containing solution. Current amplitude was taken at the beginning of the brief depolarizing pulse from −80 to −10 mV. Each bar indicates the mean ± SEM (n = 8 for each bar). Of notice, the peak I Na in GH 3 cells is subject to inhibition by TTX but not by ω-conotoxin MVIID. * Significantly different from control ( p < 0.05) and ** Significantly different from GV-58 (3 μM) alone group ( p < 0.05). Statistical analyses were made by one-way ANOVA among different groups ( p < 0.05).

    Article Snippet: GV-58 (1402821-41-3, CHEMBL2206242, SCHEMBL17628602, (2 R )-2-[(6-{[(5-methylthiophen-2-yl)methyl]amino}-9-propyl-9H-purin-2-yl)amino]butan-1-ol, C 18 H 26 N 6 OS, https://pubchem.ncbi.nlm.nih.gov/compound/71463101 , accessed on 1 December 2022), ω-conotoxin MVIID, and tetrodotoxin (TTX) were acquired from Alomone Labs (Genechain, Kaohsiung, Taiwan), while 4-aminopyridine, E-4031, nimodipine, ranolazine, retinoic acid, riluzole, and tetraethylammonium chloride (TEA) were from Sigma-Aldrich (Merck, Taipei, Taiwan).

    Techniques: Inhibition

    Effects of GV−58 on spontaneous action potentials (APs) recorded from GH 3 cells. Cells were bathed in normal Tyrode’s solution, and the recording pipettes were filled with K + −containing solution. When whole−cell configuration was established, we switched to the whole−cell current clamp recordings to measure changes in membrane potential, as current was set at zero. ( A ) Representative potential traces achieved in the absence (upper) and presence of 1 μM GV−58 (middle) or 3 μM GV−58 (lower). ( B ) Summary graph demonstrating effect of ω−conotoxin MVIID, GV−58, and GV−58 plus ranolazine (Ran) on the firing frequency of APs in GH 3 cells (mean ± SEM; n = 8). * Significantly different from control ( p < 0.05) and ** Significantly different from GV−58 (3 μM) alone group ( p < 0.05). Statistical analyses were made in one−way ANOVA among different groups ( p < 0.05).

    Journal: Biomedicines

    Article Title: Activation of Voltage-Gated Na + Current by GV-58, a Known Activator of Ca V Channels

    doi: 10.3390/biomedicines10030721

    Figure Lengend Snippet: Effects of GV−58 on spontaneous action potentials (APs) recorded from GH 3 cells. Cells were bathed in normal Tyrode’s solution, and the recording pipettes were filled with K + −containing solution. When whole−cell configuration was established, we switched to the whole−cell current clamp recordings to measure changes in membrane potential, as current was set at zero. ( A ) Representative potential traces achieved in the absence (upper) and presence of 1 μM GV−58 (middle) or 3 μM GV−58 (lower). ( B ) Summary graph demonstrating effect of ω−conotoxin MVIID, GV−58, and GV−58 plus ranolazine (Ran) on the firing frequency of APs in GH 3 cells (mean ± SEM; n = 8). * Significantly different from control ( p < 0.05) and ** Significantly different from GV−58 (3 μM) alone group ( p < 0.05). Statistical analyses were made in one−way ANOVA among different groups ( p < 0.05).

    Article Snippet: GV-58 (1402821-41-3, CHEMBL2206242, SCHEMBL17628602, (2 R )-2-[(6-{[(5-methylthiophen-2-yl)methyl]amino}-9-propyl-9H-purin-2-yl)amino]butan-1-ol, C 18 H 26 N 6 OS, https://pubchem.ncbi.nlm.nih.gov/compound/71463101 , accessed on 1 December 2022), ω-conotoxin MVIID, and tetrodotoxin (TTX) were acquired from Alomone Labs (Genechain, Kaohsiung, Taiwan), while 4-aminopyridine, E-4031, nimodipine, ranolazine, retinoic acid, riluzole, and tetraethylammonium chloride (TEA) were from Sigma-Aldrich (Merck, Taipei, Taiwan).

    Techniques: