Review




Structured Review

IMEM Inc diffquick staining
( A–D ) BALB/c mice were infected with PR8 (10LD 50 ) i.n.. Cells were collected from 4 dpi animals ( A ) BAL fluid flow profile of percent of neutrophils (Ly6G + CD11b hi ). Cells gated previously as CD45 + Thy1.2 − . ( B ) <t>DiffQuick</t> staining of sorted neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ) ( C ) Representative flow profile of HA + NP + neutrophils at 4 dpi (Cells gated as CD45 + Thy1.2 − Ly6G + CD11b hi ). B/Lee infected mice were utilized to set flow cytometric gate. ( D ) Influenza M2 mRNA expression (relative to GAPDH) from sorted HA + and HA − neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ). Table is representative of one experiment. Additional experiment yielded similar results. ( A–D ) Two independent experiments (n = 2–4/expt) represented as mean ± SEM (where applicable).
Diffquick Staining, supplied by IMEM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stain+diffquick/diffquick+staining/pmc03466305-153-6-8
Average 90 stars, based on 1 article reviews
diffquick staining - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Influenza-Infected Neutrophils within the Infected Lungs Act as Antigen Presenting Cells for Anti-Viral CD8 + T Cells"

Article Title: Influenza-Infected Neutrophils within the Infected Lungs Act as Antigen Presenting Cells for Anti-Viral CD8 + T Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0046581

( A–D ) BALB/c mice were infected with PR8 (10LD 50 ) i.n.. Cells were collected from 4 dpi animals ( A ) BAL fluid flow profile of percent of neutrophils (Ly6G + CD11b hi ). Cells gated previously as CD45 + Thy1.2 − . ( B ) DiffQuick staining of sorted neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ) ( C ) Representative flow profile of HA + NP + neutrophils at 4 dpi (Cells gated as CD45 + Thy1.2 − Ly6G + CD11b hi ). B/Lee infected mice were utilized to set flow cytometric gate. ( D ) Influenza M2 mRNA expression (relative to GAPDH) from sorted HA + and HA − neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ). Table is representative of one experiment. Additional experiment yielded similar results. ( A–D ) Two independent experiments (n = 2–4/expt) represented as mean ± SEM (where applicable).
Figure Legend Snippet: ( A–D ) BALB/c mice were infected with PR8 (10LD 50 ) i.n.. Cells were collected from 4 dpi animals ( A ) BAL fluid flow profile of percent of neutrophils (Ly6G + CD11b hi ). Cells gated previously as CD45 + Thy1.2 − . ( B ) DiffQuick staining of sorted neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ) ( C ) Representative flow profile of HA + NP + neutrophils at 4 dpi (Cells gated as CD45 + Thy1.2 − Ly6G + CD11b hi ). B/Lee infected mice were utilized to set flow cytometric gate. ( D ) Influenza M2 mRNA expression (relative to GAPDH) from sorted HA + and HA − neutrophils (CD45 + Ly6G + Ly6C int CD11b hi ). Table is representative of one experiment. Additional experiment yielded similar results. ( A–D ) Two independent experiments (n = 2–4/expt) represented as mean ± SEM (where applicable).

Techniques Used: Infection, Staining, Hi-C, Expressing

Related Articles

Staining:

Article Title: Influenza-Infected Neutrophils within the Infected Lungs Act as Antigen Presenting Cells for Anti-Viral CD8 + T Cells
Article Snippet: .. Purity of neutrophils was confirmed by DiffQuick staining (IMEM Inc.) as per manufacturers' instructions. ..



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