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quantitative reverse transcriptase polymerase chain reaction qrt pcr  (Thermo Fisher)


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    Thermo Fisher quantitative reverse transcriptase polymerase chain reaction qrt pcr
    Quantitative Reverse Transcriptase Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single-cell+spatial+transcriptomics+data/Single+Cell-to-CT+qRT-PCR+Kit/pmc07858242-1264-0-8
    Average 98 stars, based on 888 article reviews
    quantitative reverse transcriptase polymerase chain reaction qrt pcr - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Vibrio cholerae Cytolysin Causes an Inflammatory Response in Human Intestinal Epithelial Cells That Is Modulated by the PrtV Protease
    Article Snippet: .. The 18S rRNA content was determined in all samples using a real-time quantitative RT-PCR kit (Applied Biosystems) and results were expressed as mRNA copies per 18S rRNA unit. .. The 18S rRNA content was determined in all samples using a real-time quantitative RT-PCR kit (Applied Biosystems) and results were expressed as mRNA copies per 18S rRNA unit.

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCode miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)
    Article Snippet: .. Total RNA was extracted from peripheral blood with commercially available kits (Qiagen, Hilden, Germany), and cDNA synthesized from 1 μ g of RNA with SuperScript III First-Strand Synthesis SuperMix for qRT-PCR Kit (Invitrogen) according to manufacturer's instructions. .. NPSR1 messenger RNA (mRNA) expression levels were measured by performing quantitative real-time PCR in an ABI Prism 7500 Fast Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with SYBRGreen mix according to the manufacturer's instructions.

    Expressing:

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCode miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Comparison:

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    cDNA Synthesis:

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCode miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Analysis:

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCode miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Spectrophotometry:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Reverse Transcription:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
    Article Snippet: Total RNA was extracted from cells using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction. .. Three μ g of RNA from each sample was then reverse-transcribed into first-strand cDNA in 20 μ L of reaction mixture using the SuperScript First-Strand Synthesis System with the Real-time PCR kit (Invitrogen). .. Measurements were performed by an ABI PRISM 9700 HT sequence detection system (Applied Biosystems, Foster City, CA) using a predeveloped Taqman probe/primer combination for M1-related genes and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from the same cDNA samples.

    Real-time Polymerase Chain Reaction:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
    Article Snippet: Total RNA was extracted from cells using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction. .. Three μ g of RNA from each sample was then reverse-transcribed into first-strand cDNA in 20 μ L of reaction mixture using the SuperScript First-Strand Synthesis System with the Real-time PCR kit (Invitrogen). .. Measurements were performed by an ABI PRISM 9700 HT sequence detection system (Applied Biosystems, Foster City, CA) using a predeveloped Taqman probe/primer combination for M1-related genes and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from the same cDNA samples.

    other:


    Polymerase Chain Reaction:

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Synthesized:

    Article Title: Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)
    Article Snippet: .. Total RNA was extracted from peripheral blood with commercially available kits (Qiagen, Hilden, Germany), and cDNA synthesized from 1 μ g of RNA with SuperScript III First-Strand Synthesis SuperMix for qRT-PCR Kit (Invitrogen) according to manufacturer's instructions. .. NPSR1 messenger RNA (mRNA) expression levels were measured by performing quantitative real-time PCR in an ABI Prism 7500 Fast Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with SYBRGreen mix according to the manufacturer's instructions.



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    Image Search Results


    (a) Simplified cross-section of the human epidermis, highlighting squamous cells, melanocytes and basal cells. Coloured regions represent cSCC (green), which originates from squamous cells, melanoma (orange), which originates from melanocytes, and BCC (blue), which originates from basal cells. Two orange melanocytes are shown in the dermal region as occurs in invasive melanoma; other cells in the lower dermis layer are not depicted. (b) Overview of sample design and technologies used to generate data for this project. ROI - region of interest; FOV - field of view; S - cSCC; B - BCC; M - melanoma; HC - healthy (cancer patient); HNC - healthy (non-cancer patient donor). Technologies included are single cell RNA sequencing for fresh samples, single nuclei sequencing for formalin-fixed samples, Visium, Xenium, CosMX, GeoMX DSP for whole transcriptome, GeoMX DSP for proteins, Polaris, RNAscope, the proximal ligation assay, spatial glycomics and CODEX.

    Journal: bioRxiv

    Article Title: Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types

    doi: 10.1101/2025.07.25.666708

    Figure Lengend Snippet: (a) Simplified cross-section of the human epidermis, highlighting squamous cells, melanocytes and basal cells. Coloured regions represent cSCC (green), which originates from squamous cells, melanoma (orange), which originates from melanocytes, and BCC (blue), which originates from basal cells. Two orange melanocytes are shown in the dermal region as occurs in invasive melanoma; other cells in the lower dermis layer are not depicted. (b) Overview of sample design and technologies used to generate data for this project. ROI - region of interest; FOV - field of view; S - cSCC; B - BCC; M - melanoma; HC - healthy (cancer patient); HNC - healthy (non-cancer patient donor). Technologies included are single cell RNA sequencing for fresh samples, single nuclei sequencing for formalin-fixed samples, Visium, Xenium, CosMX, GeoMX DSP for whole transcriptome, GeoMX DSP for proteins, Polaris, RNAscope, the proximal ligation assay, spatial glycomics and CODEX.

    Article Snippet: Cells expressing the two genes are visualized on single-cell level resolution spatial data from STOmics and Curio-Seeker (Takara Bio, USA) melanoma samples and appear to be in spatial proximity ( ).

    Techniques: RNA Sequencing, Sequencing, RNAscope, Ligation

    (a) Gene specificity score (GSS) and association of spatial spots with skin cancer heritability. GSS score for each gene in a spot/cell represents the enrichment of the gene as a top rank most abundant gene in the spot/cell and its neighbour spots/cells in an anatomical region, a spatial domain, or a cell type. The p-value shows the spatial heritability enrichment significance of a spot with a trait based on SNPs mapped to the genes with high GSS scores (one-sided Z-test for stratified coefficient different to 0). The p-value is more significant if the SNPs that are mapped to the high GSS genes explain a higher proportion of heritability for the trait. (b) Cell types with the highest enrichment of heritability explained by SNPs tagged to GSS genes of cells in a cell type. The white asterisks indicate the most enriched cell-type for heritability of cutaneous melanoma, cSCC and BCC traits. (c) gsMAP significance spatial heritability enrichment is shown at single-cell resolution across the tissue (upper tissue plots) or per annotated skin regions (lower violin plots) from the cosMx data of the sample mel48974. (d) LR pairs with significant association with SNP heritability explained by the corresponding cell types. The rectangles show cases where both L and R genes had PCC >0.3 between GSS of the gene and the gsMAP P-values (the significance level for the LD stratified coefficients for the spot bigger than 0). The results suggest which LR pairs are related with the heritability of a cell type pairs. (e) GSS of two LR pairs showing specificity of the L and R genes to tissue regions at the immune-rich dermal layers and the epidermis of the skin. (f) Manhattan plot showing top significant GWAS SNPs co-localizing with genes in melanocytes (red) and T cells (blue) that had the highest Pearson correlation between GSS and the gsMAP trait association P-value or associated with SNPs with genome-wide significance. The Y-axis shows the -log(P-value) from GWAS analysis.

    Journal: bioRxiv

    Article Title: Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types

    doi: 10.1101/2025.07.25.666708

    Figure Lengend Snippet: (a) Gene specificity score (GSS) and association of spatial spots with skin cancer heritability. GSS score for each gene in a spot/cell represents the enrichment of the gene as a top rank most abundant gene in the spot/cell and its neighbour spots/cells in an anatomical region, a spatial domain, or a cell type. The p-value shows the spatial heritability enrichment significance of a spot with a trait based on SNPs mapped to the genes with high GSS scores (one-sided Z-test for stratified coefficient different to 0). The p-value is more significant if the SNPs that are mapped to the high GSS genes explain a higher proportion of heritability for the trait. (b) Cell types with the highest enrichment of heritability explained by SNPs tagged to GSS genes of cells in a cell type. The white asterisks indicate the most enriched cell-type for heritability of cutaneous melanoma, cSCC and BCC traits. (c) gsMAP significance spatial heritability enrichment is shown at single-cell resolution across the tissue (upper tissue plots) or per annotated skin regions (lower violin plots) from the cosMx data of the sample mel48974. (d) LR pairs with significant association with SNP heritability explained by the corresponding cell types. The rectangles show cases where both L and R genes had PCC >0.3 between GSS of the gene and the gsMAP P-values (the significance level for the LD stratified coefficients for the spot bigger than 0). The results suggest which LR pairs are related with the heritability of a cell type pairs. (e) GSS of two LR pairs showing specificity of the L and R genes to tissue regions at the immune-rich dermal layers and the epidermis of the skin. (f) Manhattan plot showing top significant GWAS SNPs co-localizing with genes in melanocytes (red) and T cells (blue) that had the highest Pearson correlation between GSS and the gsMAP trait association P-value or associated with SNPs with genome-wide significance. The Y-axis shows the -log(P-value) from GWAS analysis.

    Article Snippet: Cells expressing the two genes are visualized on single-cell level resolution spatial data from STOmics and Curio-Seeker (Takara Bio, USA) melanoma samples and appear to be in spatial proximity ( ).

    Techniques: Genome Wide

    Journal: Cell

    Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution

    doi: 10.1016/j.cell.2020.12.016

    Figure Lengend Snippet:

    Article Snippet: STAR-FINDer Single Cell and Spatial Transcriptomics Data Portal , This study , https://simmonslab.shinyapps.io/FetalAtlasDataPortal.

    Techniques: Conjugation Assay, Recombinant, Saline, Modification, Plasmid Preparation, Gene Expression, RNAscope, Generated, Software