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runx2 s 19  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology runx2 s 19
    Runx2 S 19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/runx2+s+19/RUNX2+Antibody/pmc07261149-55-27-30
    Average 96 stars, based on 1560 article reviews
    runx2 s 19 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: Protein concentration was determined with a BCA assay kit (Thermo Fisher Scientific).

    Blocking Assay:

    Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells.
    Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: anti-RUNX2 mAb (1:1000, sc-390351, Santa Cruz Biotechnology), anti-Histidine mAb (1:2500, Ab18184, Abcam) and anti-14-3-3γ polyAb (1:5000) (35). .. An anti-β-Tubulin mAb (1:1000, T-4026, Sigma Aldrich) was used as loading control.

    Incubation:

    Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells.
    Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: anti-RUNX2 mAb (1:1000, sc-390351, Santa Cruz Biotechnology), anti-Histidine mAb (1:2500, Ab18184, Abcam) and anti-14-3-3γ polyAb (1:5000) (35). .. An anti-β-Tubulin mAb (1:1000, T-4026, Sigma Aldrich) was used as loading control.

    Article Title: SERCA2 dysfunction drives vascular calcification via coupling with TSPO-MCU at mitochondria-associated endoplasmic reticulum membranes.
    Article Snippet: The VSMC or histology were subsequently incubated with immunofluorescence staining blocking solution (Beyotime, Cat# P0260) for 1 h at room temperature. .. The samples were then incubated with primary antibodies against SERCA2 C674-SO3H (Bethyl Laboratories, Cat# A300-BL2103, 1:200), Runx2 (Santa Cruz, Cat# sc-101145, 1:50), and SM22α (Proteintech, Cat# 10493–1-AP, 1:200) at 4◦C overnight. ..

    Article Title: Lactobacillus fermentum induces osteoblast differentiation via activation of p38 MAPK
    Article Snippet: The samples were separated on 10%–12% SDS‒PAGE gels and transferred to nitrocellulose membranes (Bio-Rad). .. The membranes were blocked for 1 h with 0.05% Tween-20 and 3% BSA and then incubated overnight at 4 °C with primary antibodies (1:1,000) against phospho-p38 MAPK, p38 MAPK, ALP, type I collagen, bone sialoprotein (CST), Osteocalcin, RUNX2, Osterix, Dlx5, β-actin (Santa Cruz), Ang1, ZO-1, Occludin, Claudin1, and Claudin4 (Abcam). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch).

    Article Title: XCR1 + Conventional Type 1 Dendritic Cells Exacerbate the Inflammation in Osteogenic Arthritis Through IL-17A + CD8 + T Cells.
    Article Snippet: Objective.. Ankylosing spondylitis (AS) and enthesitis-related arthritis (ERA) are autoimmune bone diseases characterized by prominent heterotopic ossification and both have poor prognoses.. The pathologic mechanisms of these diseases remain poorly understood.

    Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration
    Article Snippet: .. Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, RUNX2 (1:150, Cat. sc390351, Santa Cruz Biotechnology, USA) and OCN (1:150, Cat. sc390877, Santa Cruz Biotechnology, USA) for osteogenesis markers, VEGF (1:50, Cat. sc57496, Santa Cruz Biotechnology, USA) and CD31 (1:50, Cat. sc20071, Santa Cruz Biotechnology, USA) for angiogenesis markers, and CD146 (1:200, Cat. Ab75769, Abcam, UK) for stem cell surface marker, overnight at 4 °C. ..

    Gentle:

    Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells.
    Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: anti-RUNX2 mAb (1:1000, sc-390351, Santa Cruz Biotechnology), anti-Histidine mAb (1:2500, Ab18184, Abcam) and anti-14-3-3γ polyAb (1:5000) (35). .. An anti-β-Tubulin mAb (1:1000, T-4026, Sigma Aldrich) was used as loading control.

    Cell-Signaling:

    Article Title: The Role of Runx2 in Microtubule Dynamics and Its Effects on Osteoblast Migration.
    Article Snippet: The migration of osteoblasts (OBs) is crucial for bone formation, remodeling, and healing.. This requires the coordinated activity of cytoskeletal components, including microtubules (MTs).. MTs complement actin filaments by regulating focal adhesion turnover and facilitating the delivery of essential proteins and cargo.

    ALP Assay:

    Article Title: Lactobacillus fermentum induces osteoblast differentiation via activation of p38 MAPK
    Article Snippet: The samples were separated on 10%–12% SDS‒PAGE gels and transferred to nitrocellulose membranes (Bio-Rad). .. The membranes were blocked for 1 h with 0.05% Tween-20 and 3% BSA and then incubated overnight at 4 °C with primary antibodies (1:1,000) against phospho-p38 MAPK, p38 MAPK, ALP, type I collagen, bone sialoprotein (CST), Osteocalcin, RUNX2, Osterix, Dlx5, β-actin (Santa Cruz), Ang1, ZO-1, Occludin, Claudin1, and Claudin4 (Abcam). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (1:10,000; Jackson ImmunoResearch).

    Marker:

    Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration
    Article Snippet: .. Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, RUNX2 (1:150, Cat. sc390351, Santa Cruz Biotechnology, USA) and OCN (1:150, Cat. sc390877, Santa Cruz Biotechnology, USA) for osteogenesis markers, VEGF (1:50, Cat. sc57496, Santa Cruz Biotechnology, USA) and CD31 (1:50, Cat. sc20071, Santa Cruz Biotechnology, USA) for angiogenesis markers, and CD146 (1:200, Cat. Ab75769, Abcam, UK) for stem cell surface marker, overnight at 4 °C. ..



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    Oligodeoxynucleotide primers used for qRT-PCR.
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    Image Search Results


    TABLE 1

    Journal: The Journal of Biological Chemistry

    Article Title: Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B *

    doi: 10.1074/jbc.M115.657825

    Figure Lengend Snippet: TABLE 1

    Article Snippet: Primaryantibodies used were the following: αTFIIB C-18 (sc-225, Santa Cruz Biotechnology), RNA-PolII N-20 (sc-899, Santa Cruz Biotechnology), Runx2 S-19 (sc-12488, Santa Cruz Biotechnology), WDR5 (ab56919, Abcam), NO66 3354c5a (sc-81341, Santa Cruz Biotechnology), JARID1B (ab50958, Abcam), EZH2 (39901, Active Motif), UTX/KDM6A (ab91231, Abcam), PRMT5/JBP1 (611539, BD Biosciences), P300 N-15 (sc-584, Santa Cruz Biotechnology).

    Techniques: Expressing, Knockdown

    TABLE 1

    Journal: The Journal of Biological Chemistry

    Article Title: Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B *

    doi: 10.1074/jbc.M115.657825

    Figure Lengend Snippet: TABLE 1

    Article Snippet: Primaryantibodies used were the following: αTFIIB C-18 (sc-225, Santa Cruz Biotechnology), RNA-PolII N-20 (sc-899, Santa Cruz Biotechnology), Runx2 S-19 (sc-12488, Santa Cruz Biotechnology), WDR5 (ab56919, Abcam), NO66 3354c5a (sc-81341, Santa Cruz Biotechnology), JARID1B (ab50958, Abcam), EZH2 (39901, Active Motif), UTX/KDM6A (ab91231, Abcam), PRMT5/JBP1 (611539, BD Biosciences), P300 N-15 (sc-584, Santa Cruz Biotechnology).

    Techniques: Expressing

    Oligodeoxynucleotide primers used for qRT-PCR.

    Journal: PLoS ONE

    Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

    doi: 10.1371/journal.pone.0068926

    Figure Lengend Snippet: Oligodeoxynucleotide primers used for qRT-PCR.

    Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques:

    (A). hPDL cells were cultured in the presence and absence of daily LIPUS stimulation. BMP-2 mRNA expression was determined using qRT-PCR. (B). BMP-2 protein (ROW 1; 45 kDa) can be detected in the control and LIPUS groups (1: CON; 2: LIPUS). The LIPUS groups showed higher expression of BMP-2 from day 5. (C). Quantification of BMP-2 protein expression in the LIPUS stimulation group was greater than that in the control group on days 5, 7, and 14. *indicates P<0.05 . (D–F). Rat upper first molars were stimulated with or without LIPUS for different time intervals, and HGF, Runx2, BMP-2 were determined by qRT-PCR and Western blot, respectively. The data indicated that LIPUS increased HGF, Runx2, and BMP-2 mRNA (D: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) and protein expression (E, F: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) in vivo . The data are the mean ± SD of three separate experiments.

    Journal: PLoS ONE

    Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

    doi: 10.1371/journal.pone.0068926

    Figure Lengend Snippet: (A). hPDL cells were cultured in the presence and absence of daily LIPUS stimulation. BMP-2 mRNA expression was determined using qRT-PCR. (B). BMP-2 protein (ROW 1; 45 kDa) can be detected in the control and LIPUS groups (1: CON; 2: LIPUS). The LIPUS groups showed higher expression of BMP-2 from day 5. (C). Quantification of BMP-2 protein expression in the LIPUS stimulation group was greater than that in the control group on days 5, 7, and 14. *indicates P<0.05 . (D–F). Rat upper first molars were stimulated with or without LIPUS for different time intervals, and HGF, Runx2, BMP-2 were determined by qRT-PCR and Western blot, respectively. The data indicated that LIPUS increased HGF, Runx2, and BMP-2 mRNA (D: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) and protein expression (E, F: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01 ) in vivo . The data are the mean ± SD of three separate experiments.

    Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Control, Western Blot, In Vivo

    (A). hPDL cells were incubated with HGF for 24 h, and BMP-2 mRNA was examined by qRT-PCR. (B and C) hPDL cells were incubated with HGF for 48 h, and BMP-2 protein amounts were detected by Western blotting. The data shows that HGF significantly increased BMP-2 expression. (D). Cells were transfected with Runx2 siRNA for 24 h followed by stimulation with LIPUS for 5 days, and BMP-2 mRNA expression was examined by qRT-PCR. (E and F) hPDL cells were transfected with Runx2 siRNA for 5 days, and BMP-2 protein expression was examined by Western blot. Transfection of cells with Runx2 siRNA reduced LIPUS-increased BMP-2 expression. * P<0.05 (** P<0.01 ) as compared with the control group.

    Journal: PLoS ONE

    Article Title: Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway

    doi: 10.1371/journal.pone.0068926

    Figure Lengend Snippet: (A). hPDL cells were incubated with HGF for 24 h, and BMP-2 mRNA was examined by qRT-PCR. (B and C) hPDL cells were incubated with HGF for 48 h, and BMP-2 protein amounts were detected by Western blotting. The data shows that HGF significantly increased BMP-2 expression. (D). Cells were transfected with Runx2 siRNA for 24 h followed by stimulation with LIPUS for 5 days, and BMP-2 mRNA expression was examined by qRT-PCR. (E and F) hPDL cells were transfected with Runx2 siRNA for 5 days, and BMP-2 protein expression was examined by Western blot. Transfection of cells with Runx2 siRNA reduced LIPUS-increased BMP-2 expression. * P<0.05 (** P<0.01 ) as compared with the control group.

    Article Snippet: Primary α-tubulin antibody was purchased from Millipore (MAB5566, Billerica, MA, USA); anti-β-actin (N-21), anti-HGF (H-170), and anti-Runx2 (S-19) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Incubation, Quantitative RT-PCR, Western Blot, Expressing, Transfection, Control