runx2 s 19 (Santa Cruz Biotechnology)
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Runx2 S 19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/runx2+s+19/RUNX2+Antibody/pmc07261149-55-27-30
Average 96 stars, based on 1560 article reviews
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other:Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D Article Snippet: Protein concentration was determined with a BCA assay kit (Thermo Fisher Scientific). Blocking Assay:Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells. Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: Incubation:Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells. Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: Article Title: SERCA2 dysfunction drives vascular calcification via coupling with TSPO-MCU at mitochondria-associated endoplasmic reticulum membranes. Article Snippet: The VSMC or histology were subsequently incubated with immunofluorescence staining blocking solution (Beyotime, Cat# P0260) for 1 h at room temperature. .. The samples were then incubated with primary antibodies against SERCA2 C674-SO3H (Bethyl Laboratories, Cat# A300-BL2103, 1:200), Article Title: Lactobacillus fermentum induces osteoblast differentiation via activation of p38 MAPK Article Snippet: The samples were separated on 10%–12% SDS‒PAGE gels and transferred to nitrocellulose membranes (Bio-Rad). .. The membranes were blocked for 1 h with 0.05% Tween-20 and 3% BSA and then incubated overnight at 4 °C with primary antibodies (1:1,000) against phospho-p38 MAPK, p38 MAPK, ALP, type I collagen, bone sialoprotein (CST), Osteocalcin, Article Title: XCR1 + Conventional Type 1 Dendritic Cells Exacerbate the Inflammation in Osteogenic Arthritis Through IL-17A + CD8 + T Cells. Article Snippet: Objective.. Ankylosing spondylitis (AS) and enthesitis-related arthritis (ERA) are autoimmune bone diseases characterized by prominent heterotopic ossification and both have poor prognoses.. The pathologic mechanisms of these diseases remain poorly understood. Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration Article Snippet: .. Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, Gentle:Article Title: 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells. Article Snippet: .. After blocking with 5% skimmed milk in PBS for 1 hour at room temperature, membranes were incubated overnight under gentle agitation at 4°C with the following primary antibodies: Cell-Signaling:Article Title: The Role of Runx2 in Microtubule Dynamics and Its Effects on Osteoblast Migration. Article Snippet: The migration of osteoblasts (OBs) is crucial for bone formation, remodeling, and healing.. This requires the coordinated activity of cytoskeletal components, including microtubules (MTs).. MTs complement actin filaments by regulating focal adhesion turnover and facilitating the delivery of essential proteins and cargo. ALP Assay:Article Title: Lactobacillus fermentum induces osteoblast differentiation via activation of p38 MAPK Article Snippet: The samples were separated on 10%–12% SDS‒PAGE gels and transferred to nitrocellulose membranes (Bio-Rad). .. The membranes were blocked for 1 h with 0.05% Tween-20 and 3% BSA and then incubated overnight at 4 °C with primary antibodies (1:1,000) against phospho-p38 MAPK, p38 MAPK, ALP, type I collagen, bone sialoprotein (CST), Osteocalcin, Marker:Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration Article Snippet: .. Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, |

