reverse transcriptase reaction kit (Promega)
90
Structured Review
Promega
reverse transcriptase reaction kit
Reverse Transcriptase Reaction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+reaction/reverse+transcriptase+reaction+kit/pmc06496449-55-20-24
Average 90 stars, based on 1 article reviews
Reverse Transcriptase Reaction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+reaction/reverse+transcriptase+reaction+kit/pmc06496449-55-20-24
Average 90 stars, based on 1 article reviews
reverse transcriptase reaction kit - by Bioz Stars,
2026-10
90/100 stars
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cDNA Synthesis:Article Title: RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression. Article Snippet: Total RNA was isolated using the TRIzol reagent (Life Technologies Inc.) following manufacturer’s instructions. .. One microgram RNA was used for cDNA synthesis using a Article Title: KIF18A is a novel target of JNK1/c-Jun signaling pathway involved in tumorigenesis Article Snippet: RNA Isolation and Quantitative Real-time PCR Total RNAs were extracted from siRNAs or plasmids-transfected cells utilizing TRIZOL reagent (Invitrogen, USA). .. One microgram RNA was used for cDNA synthesis with the aid of a Reverse Transcription:Article Title: RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression. Article Snippet: Total RNA was isolated using the TRIzol reagent (Life Technologies Inc.) following manufacturer’s instructions. .. One microgram RNA was used for cDNA synthesis using a Article Title: HOXA13 serves as a biomarker to predict neoadjuvant therapy efficacy in advanced colorectal cancer patients. Article Snippet: DDP (Solarbio, Beijing, China) and 5- FU (Merck Millipore) were dissolved in the corresponding medium at the indicated doses, and the cells were treated for the indicated time. .. Quantitative real-time PCR (qRT-PCR) RNA extraction and reverse transcription reactions were performed as described previously [15] using a Article Title: Mycobacterium tuberculosis transcriptional regulator Rv1019 is upregulated in hypoxia, and negatively regulates Rv3230c-Rv3229c operon encoding enzymes in the oleic acid biosynthetic pathway. Article Snippet: .. RNA was resuspended in nuclease-free water and treated with DNase I. Complementary DNA (cDNA) was prepared using Article Title: KIF18A is a novel target of JNK1/c-Jun signaling pathway involved in tumorigenesis Article Snippet: RNA Isolation and Quantitative Real-time PCR Total RNAs were extracted from siRNAs or plasmids-transfected cells utilizing TRIZOL reagent (Invitrogen, USA). .. One microgram RNA was used for cDNA synthesis with the aid of a Article Title: A Phosphate-Starvation Enhanced Purple Acid Phosphatase, GmPAP23 Mediates Intracellular Phosphorus Recycling and Yield in Soybean. Article Snippet: Plant internal phosphorus (P) recycling is a complex process, which is vital for improving plant P use efficiency.. However, the mechanisms underlying phosphate (Pi) release from internal organic‐P form remains to be deciphered in crops.. Here, we functionally characterised a Pi‐starvation responsive purple acid phosphatase (PAP), GmPAP23 in soybean (Glycine max). Article Title: Lipid exchange at ER–trans-Golgi contact sites governs polarized cargo sorting Article Snippet: Cluster scattering was quantified by measuring object perimeters over time-lapse imaging by CellProfiler 4.1.3 image analyses software. .. To analyze the mRNA expression of selected genes, 0.2 × 10 6 A549 cells were seeded into 6 cm–diameter culture dishes and then EMT was induced with 5 ng/ml TGF-β for 72 h. Total RNA was purified using the RNeasy Plus Mini kit (Qiagen) and a total amount of 2 μg RNA was reverse transcribed with a GO Article Title: Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1. Article Snippet: .. Isolation, cloning, and plasmid construction for PHS1 and BGC1 Total RNA was extracted from leaves of 2-wk-old wheat seedlings using RNeasy kit (Qiagen) with on-column DNase I digestion (Qiagen). cDNA was synthesized (using 2 μg RNA) 4104 | THE PLANT CELL 2023: 35; 4091–4110 Kamble et al. D ow nloaded from https://academ ic.oup.com /plcell/article/35/11/4091/7246009 by guest on 19 January 2024 using the Real-time Polymerase Chain Reaction:Article Title: RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression. Article Snippet: Total RNA was isolated using the TRIzol reagent (Life Technologies Inc.) following manufacturer’s instructions. .. One microgram RNA was used for cDNA synthesis using a Article Title: HOXA13 serves as a biomarker to predict neoadjuvant therapy efficacy in advanced colorectal cancer patients. Article Snippet: DDP (Solarbio, Beijing, China) and 5- FU (Merck Millipore) were dissolved in the corresponding medium at the indicated doses, and the cells were treated for the indicated time. .. Quantitative real-time PCR (qRT-PCR) RNA extraction and reverse transcription reactions were performed as described previously [15] using a Article Title: KIF18A is a novel target of JNK1/c-Jun signaling pathway involved in tumorigenesis Article Snippet: RNA Isolation and Quantitative Real-time PCR Total RNAs were extracted from siRNAs or plasmids-transfected cells utilizing TRIZOL reagent (Invitrogen, USA). .. One microgram RNA was used for cDNA synthesis with the aid of a SYBR Green Assay:Article Title: RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression. Article Snippet: Total RNA was isolated using the TRIzol reagent (Life Technologies Inc.) following manufacturer’s instructions. .. One microgram RNA was used for cDNA synthesis using a Article Title: HOXA13 serves as a biomarker to predict neoadjuvant therapy efficacy in advanced colorectal cancer patients. Article Snippet: DDP (Solarbio, Beijing, China) and 5- FU (Merck Millipore) were dissolved in the corresponding medium at the indicated doses, and the cells were treated for the indicated time. .. Quantitative real-time PCR (qRT-PCR) RNA extraction and reverse transcription reactions were performed as described previously [15] using a Article Title: KIF18A is a novel target of JNK1/c-Jun signaling pathway involved in tumorigenesis Article Snippet: RNA Isolation and Quantitative Real-time PCR Total RNAs were extracted from siRNAs or plasmids-transfected cells utilizing TRIZOL reagent (Invitrogen, USA). .. One microgram RNA was used for cDNA synthesis with the aid of a RNA Extraction:Article Title: HOXA13 serves as a biomarker to predict neoadjuvant therapy efficacy in advanced colorectal cancer patients. Article Snippet: DDP (Solarbio, Beijing, China) and 5- FU (Merck Millipore) were dissolved in the corresponding medium at the indicated doses, and the cells were treated for the indicated time. .. Quantitative real-time PCR (qRT-PCR) RNA extraction and reverse transcription reactions were performed as described previously [15] using a Sequencing:Article Title: KIF18A is a novel target of JNK1/c-Jun signaling pathway involved in tumorigenesis Article Snippet: RNA Isolation and Quantitative Real-time PCR Total RNAs were extracted from siRNAs or plasmids-transfected cells utilizing TRIZOL reagent (Invitrogen, USA). .. One microgram RNA was used for cDNA synthesis with the aid of a Expressing:Article Title: Lipid exchange at ER–trans-Golgi contact sites governs polarized cargo sorting Article Snippet: Cluster scattering was quantified by measuring object perimeters over time-lapse imaging by CellProfiler 4.1.3 image analyses software. .. To analyze the mRNA expression of selected genes, 0.2 × 10 6 A549 cells were seeded into 6 cm–diameter culture dishes and then EMT was induced with 5 ng/ml TGF-β for 72 h. Total RNA was purified using the RNeasy Plus Mini kit (Qiagen) and a total amount of 2 μg RNA was reverse transcribed with a GO Purification:Article Title: Lipid exchange at ER–trans-Golgi contact sites governs polarized cargo sorting Article Snippet: Cluster scattering was quantified by measuring object perimeters over time-lapse imaging by CellProfiler 4.1.3 image analyses software. .. To analyze the mRNA expression of selected genes, 0.2 × 10 6 A549 cells were seeded into 6 cm–diameter culture dishes and then EMT was induced with 5 ng/ml TGF-β for 72 h. Total RNA was purified using the RNeasy Plus Mini kit (Qiagen) and a total amount of 2 μg RNA was reverse transcribed with a GO other:Article Title: Lipid exchange at ER-trans-Golgi contact sites governs polarized cargo sorting. Article Snippet: To analyze the mRNA expression of selected genes, 0.2 × 106 A549 cells were seeded into 6 cm–diameter culture dishes and then EMT was induced with 5 ng/ml Isolation:Article Title: Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1. Article Snippet: .. Isolation, cloning, and plasmid construction for PHS1 and BGC1 Total RNA was extracted from leaves of 2-wk-old wheat seedlings using RNeasy kit (Qiagen) with on-column DNase I digestion (Qiagen). cDNA was synthesized (using 2 μg RNA) 4104 | THE PLANT CELL 2023: 35; 4091–4110 Kamble et al. D ow nloaded from https://academ ic.oup.com /plcell/article/35/11/4091/7246009 by guest on 19 January 2024 using the Cloning:Article Title: Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1. Article Snippet: .. Isolation, cloning, and plasmid construction for PHS1 and BGC1 Total RNA was extracted from leaves of 2-wk-old wheat seedlings using RNeasy kit (Qiagen) with on-column DNase I digestion (Qiagen). cDNA was synthesized (using 2 μg RNA) 4104 | THE PLANT CELL 2023: 35; 4091–4110 Kamble et al. D ow nloaded from https://academ ic.oup.com /plcell/article/35/11/4091/7246009 by guest on 19 January 2024 using the Plasmid Preparation:Article Title: Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1. Article Snippet: .. Isolation, cloning, and plasmid construction for PHS1 and BGC1 Total RNA was extracted from leaves of 2-wk-old wheat seedlings using RNeasy kit (Qiagen) with on-column DNase I digestion (Qiagen). cDNA was synthesized (using 2 μg RNA) 4104 | THE PLANT CELL 2023: 35; 4091–4110 Kamble et al. D ow nloaded from https://academ ic.oup.com /plcell/article/35/11/4091/7246009 by guest on 19 January 2024 using the Synthesized:Article Title: Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1. Article Snippet: .. Isolation, cloning, and plasmid construction for PHS1 and BGC1 Total RNA was extracted from leaves of 2-wk-old wheat seedlings using RNeasy kit (Qiagen) with on-column DNase I digestion (Qiagen). cDNA was synthesized (using 2 μg RNA) 4104 | THE PLANT CELL 2023: 35; 4091–4110 Kamble et al. D ow nloaded from https://academ ic.oup.com /plcell/article/35/11/4091/7246009 by guest on 19 January 2024 using the |