alexa fluor 568 goat anti rabbit secondary antibody (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher alexa fluor 568 goat anti rabbit secondary antibody
Alexa Fluor 568 Goat Anti Rabbit Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+secondary/Triton+X-100/pm42306957-92-18-26
Average 99 stars, based on 1 article reviews

alexa fluor 568 goat anti rabbit secondary antibody - by Bioz Stars, 2026-09

99/100 stars

Images

Incubation:

Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation
Article Snippet: PG-hiHs were retrieved and fixed using 10% neutral buffered formalin (NBF) (Sigma). .. Following fixation, the 3D colonies were rinsed with wash buffer (DPBS with Ca 2+ /Mg 2+ supplemented with 0.2% Triton-X 100 and 0.1% gelatin from cold water (all from Sigma‒Aldrich) and subsequently incubated in 10% normal goat serum (Thermo Fisher) in wash buffer overnight for blocking. ..

Article Title: The pro-inflammatory cytokines IFN-α and TNF-α inhibit organoid-derived extravillous trophoblast invasion
Article Snippet: .. For whole-mount immunostaining in a 48-wells plate, non-specific binding sites of the tissue fragments were first blocked by incubation in 1% BSA/PBS with 1% Triton X-100 and 0.1% saponin (Acros Organics, 419231000) (blocking/permeabilization buffer) overnight at 4 °C. ..

Blocking Assay:

Article Title: Neonatal muscle-derived extracellular vesicles containing miR-542-3p rejuvenate aged skeletal muscle via a functional microneedle patch
Article Snippet: .. After washing frozen muscle sections or C2C12 cell coverslips with PBS, the samples were fixed 4% paraformaldehyde (PFA, Beyotime Biotechnology, China) for 10 min. After three PBS washes, the samples were permeabilized with 0.1% Triton X-100 in PBS for 15 min, followed by blocking with 3% bovine serum albumin (BSA, Thermo Fisher, USA) for 30 min. ..

Sterility:

Article Title: Neonatal expression of human FMRP isoform corrects cortical deficits and improves behavior in a mouse model of fragile X syndrome
Article Snippet: .. First, samples were added to sterile tubes pre-filled with ceramic beads and homogenized in 300 μL NE1 buffer (20 mM HEPES, 10 mM KCl, 1 mM MgCl2, 0.1% Triton X-100, 20% glycerol, 0.5 mM DTT, 1× Pierce Complete Protease Inhibitors) using a Bead Mill 24 homogenizer (Fisher Scientific, MA, USA). .. Samples were chilled on ice; 1 μL benzonase (#E1014-5KU; Sigma, MA, USA) was added per tube, briefly mixed, and allowed to incubate at room temperature for 15 min. A fraction of the homogenate was diluted in NE1 buffer, and protein concentrations were quantified using BioRad DC II (Bio-Rad, CA, USA) on an ID3 plate reader (Molecular Devices, CA, USA).

Immunostaining:

Binding Assay:

Control:

Article Title: Tangential flow filtration for isolating exomeres and other nanoscale extracellular particles.
Article Snippet: Samples were immediately quenched with 1× EDTA-free protease inhibitor cocktail (Thermo, 78425) for 5 minutes at RT to inactivate Proteinase K, then treated with 1 μg mL−1 RNase A as before. .. For Triton X-100/RNase A treatment, Triton X-100 was added to samples at a final ratio of 0.075% (v/v), vortexed for 30 seconds, then immediately treated with 1 μg mL−1 RNase A. Optimal Triton X-100 conditions to disrupt EV membranes were determined elsewhere.28 As a control for cotreatment effects, GAPDH siRNA from the QubitTM microRNA assay kit (Thermo, Q32880) was evaluated in parallel. ..

Staining:

Article Title: Multifunctional scaffold inspired by hepatocyte exosomes promotes bone regeneration by regulating osteogenic differentiation via PI3K/AKT pathway
Article Snippet: .. Cells were then fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and stained with 488-phalloidin (1:200, Invitrogen) and Hoechst 33342 (5 μg/mL, Beyotime). .. The uptake of the labeled exosomes by the cells was imaged using a confocal microscope (Nikon, Tokyo, Japan).

Article Title: Systematic evaluation of cryopreservation parameters for natural killer cells in clinically relevant cyclic olefin polymer vials.
Article Snippet: Background aims: Allogeneic cell therapy uses donor-derived cells to treat disease indications in oncology, autoimmune disorders, and other areas.. Unlike patient-specific autologous therapies, allogeneic therapies are manufactured in large batches.. For these products, the final containment is critical for maintaining cell viability and function.

Review