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rabbit secondary antibody  (Abmart Inc)

 
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    Abmart Inc rabbit secondary antibody
    Rabbit Secondary Antibody, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+secondary/anti+secondary/pm42309446-85-3-6
    Average 86 stars, based on 1 article reviews
    rabbit secondary antibody - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Harnessing serum exosomal snoRNAs: A novel liquid biopsy approach for NSCLC diagnosis.
    Article Snippet: Three commonly enriched exosomal surface markers were detected: CD63 (Abcam, USA; 1:1000), CD81 (ProteinTech, USA; 1:1000), and CD9 (ProteinTech, USA; 1:800). .. A freshly prepared rabbit secondary antibody (Abmart; 1:5000) was used, and protein bands were visualized after incubation for 1 h at 37 ◦C with gentle shaking. ..

    Article Title: Demineralized Dentin Matrix Promotes Bone Regeneration Through IDO1-Mediated Th17/Treg Cell Balance Modulation
    Article Snippet: .. The membrane was incubated with universal secondary antibodies (Abmart) for 1 hour, followed by 3 washes with Tris-Buffered Saline with Tween 20 (TBST) for 5 minutes each. .. Finally, the membrane was dried with absorbent paper and then visualised using ECL chemiluminescence imaging system (Tanon).

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development.
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 ◦C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Article Title: Ameliorative effects and mechanisms of Inonotus hispidus on PCOS via regulation of gut microbiota-ovarian metabolism axis and ferroptosis inhibition.
    Article Snippet: Polycystic ovary syndrome (PCOS) is a prevalent endocrine metabolic disorder with limited therapeutic options.. This study investigated the ameliorative effects and underlying mechanisms of the water extract of Inonotus hispidus (WE) on PCOS in a testosterone propionate induced rat model.. The results of this study indicate that WE significantly reduced abnormal body weight gain, restored disrupted estrous cycles, and ameliorated ovarian cystic degeneration, interstitial fibrosis, and hepatic steatosis in PCOS rats.

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 °C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Gentle:

    Article Title: Harnessing serum exosomal snoRNAs: A novel liquid biopsy approach for NSCLC diagnosis.
    Article Snippet: Three commonly enriched exosomal surface markers were detected: CD63 (Abcam, USA; 1:1000), CD81 (ProteinTech, USA; 1:1000), and CD9 (ProteinTech, USA; 1:800). .. A freshly prepared rabbit secondary antibody (Abmart; 1:5000) was used, and protein bands were visualized after incubation for 1 h at 37 ◦C with gentle shaking. ..

    Western Blot:

    Article Title: The microtubule-associated protein RIC1 forms biomolecular condensates to promote the polymerization of microtubule bundles in vitro.
    Article Snippet: The Arabidopsis thaliana RIC1, a key member of the ROP (Rho GTPase of Plants)-Interactive CRIB motifcontaining protein family, has been characterized as a plant-specific microtubule-associated protein.. RIC1 is thought to function as a key downstream effector of the small GTPase Rho-of-Plant 6 (ROP6), orchestrating the formation of well-ordered cortical microtubule arrays and promoting the interdigitated growth pattern of cotyledon or leaf epidermal pavement cells.. However, the precise physicochemical properties governing RIC1's function in microtubule organization regulation remain known.

    Membrane:

    Article Title: Demineralized Dentin Matrix Promotes Bone Regeneration Through IDO1-Mediated Th17/Treg Cell Balance Modulation
    Article Snippet: .. The membrane was incubated with universal secondary antibodies (Abmart) for 1 hour, followed by 3 washes with Tris-Buffered Saline with Tween 20 (TBST) for 5 minutes each. .. Finally, the membrane was dried with absorbent paper and then visualised using ECL chemiluminescence imaging system (Tanon).

    Article Title: Flurbiprofen@ZIF-8 nanoformulation with potential for analgesia.
    Article Snippet: .. The reagents mentioned in the study and their relevant information are summarized as follows: 2-methylimidazole (Innochem, 98%, Cat. No. A50320), Zn(NO3)2·6H2O (AR, Aladdin, 99.0%, Cat. No. Z111703), flurbiprofen (Aladdin, 98.0%, Cat. No. F134483), methanol (Aladdin, 99.5.0%, Cat. No. M116115), fluorescein isothiocyanate (FITC, Solarbio, Cat. No. F8070), potassium bromide (KBr, 99.0%, Cat. No. P116270), deionized water (Aladdin, Cat. No. W119424), Dulbecco's modified Eagle medium (DMEM, Aladdin, Cat. No. D1372055), fetal bovine serum (FBS, Solarbio, Cat. No. 11011-8611 ), MTT solution (Solarbio, Cat. No. M1025 ), dimethyl sulfoxide (DMSO, Aladdin, 99.5%, Cat. No. D670381 ), phosphate-buffered saline (PBS, Aladdin, Cat. No.P743267), trypsin (Aladdin, Cat. No.T755301), lipopolysaccharide (LPS, Aladdin, Cat. No. L381866), RIPA lysate (Macklin, Cat. No. R917927), protease and phosphatase inhibitors (Cayman, Cat. No. 10009305), polyvinylidene fluoride membrane (PVDF membrane, Aladdin, Cat. No. P766361), anhydrous methanol (Innochem, Cat. No. A63792), 5% skim milk (Aladdin, Cat. No.N752146), primary antibodies (TNF-α, IL-1β, IL-6, Abmart, Cat. No. PY19810, PC6319, TD6087), secondary antibodies (Abmart, Cat. No. M21001), ECL chemiluminescent color developer (Aladdin, Cat. No. E266188), 4% neutral paraformaldehyde (Aladdin, Cat. No. C104188), Triton X-100 (Aladdin, Cat. No. T434386), citrate antigen retrieval solution (Aladdin, Cat. No. C752119), mounting medium with DAPI( Aladdin, Cat. No. D609734), complete Freund's adjuvant (CFA, Adamas, Cat. No. 4150869A), and indocyanine green (ICG, Innochem, 75%, Cat. No. A34187). ..

    Saline:

    Article Title: Demineralized Dentin Matrix Promotes Bone Regeneration Through IDO1-Mediated Th17/Treg Cell Balance Modulation
    Article Snippet: .. The membrane was incubated with universal secondary antibodies (Abmart) for 1 hour, followed by 3 washes with Tris-Buffered Saline with Tween 20 (TBST) for 5 minutes each. .. Finally, the membrane was dried with absorbent paper and then visualised using ECL chemiluminescence imaging system (Tanon).

    Article Title: Flurbiprofen@ZIF-8 nanoformulation with potential for analgesia.
    Article Snippet: .. The reagents mentioned in the study and their relevant information are summarized as follows: 2-methylimidazole (Innochem, 98%, Cat. No. A50320), Zn(NO3)2·6H2O (AR, Aladdin, 99.0%, Cat. No. Z111703), flurbiprofen (Aladdin, 98.0%, Cat. No. F134483), methanol (Aladdin, 99.5.0%, Cat. No. M116115), fluorescein isothiocyanate (FITC, Solarbio, Cat. No. F8070), potassium bromide (KBr, 99.0%, Cat. No. P116270), deionized water (Aladdin, Cat. No. W119424), Dulbecco's modified Eagle medium (DMEM, Aladdin, Cat. No. D1372055), fetal bovine serum (FBS, Solarbio, Cat. No. 11011-8611 ), MTT solution (Solarbio, Cat. No. M1025 ), dimethyl sulfoxide (DMSO, Aladdin, 99.5%, Cat. No. D670381 ), phosphate-buffered saline (PBS, Aladdin, Cat. No.P743267), trypsin (Aladdin, Cat. No.T755301), lipopolysaccharide (LPS, Aladdin, Cat. No. L381866), RIPA lysate (Macklin, Cat. No. R917927), protease and phosphatase inhibitors (Cayman, Cat. No. 10009305), polyvinylidene fluoride membrane (PVDF membrane, Aladdin, Cat. No. P766361), anhydrous methanol (Innochem, Cat. No. A63792), 5% skim milk (Aladdin, Cat. No.N752146), primary antibodies (TNF-α, IL-1β, IL-6, Abmart, Cat. No. PY19810, PC6319, TD6087), secondary antibodies (Abmart, Cat. No. M21001), ECL chemiluminescent color developer (Aladdin, Cat. No. E266188), 4% neutral paraformaldehyde (Aladdin, Cat. No. C104188), Triton X-100 (Aladdin, Cat. No. T434386), citrate antigen retrieval solution (Aladdin, Cat. No. C752119), mounting medium with DAPI( Aladdin, Cat. No. D609734), complete Freund's adjuvant (CFA, Adamas, Cat. No. 4150869A), and indocyanine green (ICG, Innochem, 75%, Cat. No. A34187). ..

    Staining:

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development.
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 ◦C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 °C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Microscopy:

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development.
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 ◦C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development
    Article Snippet: .. The PBS group was incubated in 1 mL PBS for 1 h, the IgG group was incubated with 2 μL FITC-conjugated Mouse IgG (Cat#M212308S, Abmart, Shanghai, China) and 1 mL PBS for 1 h, and the PVR group was incubated with 2 μL FITC-conjugated anti-human CD155 antibody (Cat#81254, CST, Shanghai, China) and 1 mL PBS for 1 h. Following incubation, the cells were washed once with PBS to remove unbound antibodies and stained with Hoechst staining solution (Biosharp, Hefei, China) at 37 °C for 20–30 min. After staining, the cells were washed twice with PBS to remove excess dye and observed at 488 nm using a Nikon A1 HD25 laser confocal microscope (Nikon, Minato Ward, Japan). ..

    Modification:

    Article Title: Flurbiprofen@ZIF-8 nanoformulation with potential for analgesia.
    Article Snippet: .. The reagents mentioned in the study and their relevant information are summarized as follows: 2-methylimidazole (Innochem, 98%, Cat. No. A50320), Zn(NO3)2·6H2O (AR, Aladdin, 99.0%, Cat. No. Z111703), flurbiprofen (Aladdin, 98.0%, Cat. No. F134483), methanol (Aladdin, 99.5.0%, Cat. No. M116115), fluorescein isothiocyanate (FITC, Solarbio, Cat. No. F8070), potassium bromide (KBr, 99.0%, Cat. No. P116270), deionized water (Aladdin, Cat. No. W119424), Dulbecco's modified Eagle medium (DMEM, Aladdin, Cat. No. D1372055), fetal bovine serum (FBS, Solarbio, Cat. No. 11011-8611 ), MTT solution (Solarbio, Cat. No. M1025 ), dimethyl sulfoxide (DMSO, Aladdin, 99.5%, Cat. No. D670381 ), phosphate-buffered saline (PBS, Aladdin, Cat. No.P743267), trypsin (Aladdin, Cat. No.T755301), lipopolysaccharide (LPS, Aladdin, Cat. No. L381866), RIPA lysate (Macklin, Cat. No. R917927), protease and phosphatase inhibitors (Cayman, Cat. No. 10009305), polyvinylidene fluoride membrane (PVDF membrane, Aladdin, Cat. No. P766361), anhydrous methanol (Innochem, Cat. No. A63792), 5% skim milk (Aladdin, Cat. No.N752146), primary antibodies (TNF-α, IL-1β, IL-6, Abmart, Cat. No. PY19810, PC6319, TD6087), secondary antibodies (Abmart, Cat. No. M21001), ECL chemiluminescent color developer (Aladdin, Cat. No. E266188), 4% neutral paraformaldehyde (Aladdin, Cat. No. C104188), Triton X-100 (Aladdin, Cat. No. T434386), citrate antigen retrieval solution (Aladdin, Cat. No. C752119), mounting medium with DAPI( Aladdin, Cat. No. D609734), complete Freund's adjuvant (CFA, Adamas, Cat. No. 4150869A), and indocyanine green (ICG, Innochem, 75%, Cat. No. A34187). ..

    MTT Assay:

    Article Title: Flurbiprofen@ZIF-8 nanoformulation with potential for analgesia.
    Article Snippet: .. The reagents mentioned in the study and their relevant information are summarized as follows: 2-methylimidazole (Innochem, 98%, Cat. No. A50320), Zn(NO3)2·6H2O (AR, Aladdin, 99.0%, Cat. No. Z111703), flurbiprofen (Aladdin, 98.0%, Cat. No. F134483), methanol (Aladdin, 99.5.0%, Cat. No. M116115), fluorescein isothiocyanate (FITC, Solarbio, Cat. No. F8070), potassium bromide (KBr, 99.0%, Cat. No. P116270), deionized water (Aladdin, Cat. No. W119424), Dulbecco's modified Eagle medium (DMEM, Aladdin, Cat. No. D1372055), fetal bovine serum (FBS, Solarbio, Cat. No. 11011-8611 ), MTT solution (Solarbio, Cat. No. M1025 ), dimethyl sulfoxide (DMSO, Aladdin, 99.5%, Cat. No. D670381 ), phosphate-buffered saline (PBS, Aladdin, Cat. No.P743267), trypsin (Aladdin, Cat. No.T755301), lipopolysaccharide (LPS, Aladdin, Cat. No. L381866), RIPA lysate (Macklin, Cat. No. R917927), protease and phosphatase inhibitors (Cayman, Cat. No. 10009305), polyvinylidene fluoride membrane (PVDF membrane, Aladdin, Cat. No. P766361), anhydrous methanol (Innochem, Cat. No. A63792), 5% skim milk (Aladdin, Cat. No.N752146), primary antibodies (TNF-α, IL-1β, IL-6, Abmart, Cat. No. PY19810, PC6319, TD6087), secondary antibodies (Abmart, Cat. No. M21001), ECL chemiluminescent color developer (Aladdin, Cat. No. E266188), 4% neutral paraformaldehyde (Aladdin, Cat. No. C104188), Triton X-100 (Aladdin, Cat. No. T434386), citrate antigen retrieval solution (Aladdin, Cat. No. C752119), mounting medium with DAPI( Aladdin, Cat. No. D609734), complete Freund's adjuvant (CFA, Adamas, Cat. No. 4150869A), and indocyanine green (ICG, Innochem, 75%, Cat. No. A34187). ..

    Adjuvant:

    Article Title: Flurbiprofen@ZIF-8 nanoformulation with potential for analgesia.
    Article Snippet: .. The reagents mentioned in the study and their relevant information are summarized as follows: 2-methylimidazole (Innochem, 98%, Cat. No. A50320), Zn(NO3)2·6H2O (AR, Aladdin, 99.0%, Cat. No. Z111703), flurbiprofen (Aladdin, 98.0%, Cat. No. F134483), methanol (Aladdin, 99.5.0%, Cat. No. M116115), fluorescein isothiocyanate (FITC, Solarbio, Cat. No. F8070), potassium bromide (KBr, 99.0%, Cat. No. P116270), deionized water (Aladdin, Cat. No. W119424), Dulbecco's modified Eagle medium (DMEM, Aladdin, Cat. No. D1372055), fetal bovine serum (FBS, Solarbio, Cat. No. 11011-8611 ), MTT solution (Solarbio, Cat. No. M1025 ), dimethyl sulfoxide (DMSO, Aladdin, 99.5%, Cat. No. D670381 ), phosphate-buffered saline (PBS, Aladdin, Cat. No.P743267), trypsin (Aladdin, Cat. No.T755301), lipopolysaccharide (LPS, Aladdin, Cat. No. L381866), RIPA lysate (Macklin, Cat. No. R917927), protease and phosphatase inhibitors (Cayman, Cat. No. 10009305), polyvinylidene fluoride membrane (PVDF membrane, Aladdin, Cat. No. P766361), anhydrous methanol (Innochem, Cat. No. A63792), 5% skim milk (Aladdin, Cat. No.N752146), primary antibodies (TNF-α, IL-1β, IL-6, Abmart, Cat. No. PY19810, PC6319, TD6087), secondary antibodies (Abmart, Cat. No. M21001), ECL chemiluminescent color developer (Aladdin, Cat. No. E266188), 4% neutral paraformaldehyde (Aladdin, Cat. No. C104188), Triton X-100 (Aladdin, Cat. No. T434386), citrate antigen retrieval solution (Aladdin, Cat. No. C752119), mounting medium with DAPI( Aladdin, Cat. No. D609734), complete Freund's adjuvant (CFA, Adamas, Cat. No. 4150869A), and indocyanine green (ICG, Innochem, 75%, Cat. No. A34187). ..

    Amplification:

    Article Title: Functional Insights into the RCC1 Gene Family and UVR8-Mediated Regulation of Anthocyanin Biosynthesis in Grapevine.
    Article Snippet: The extracted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes following the standard protocol provided by the manufacturer (Bio-Rad, Shanghai, China). .. A primary antibody against GFP and GADPH (Proteintech, Wuhan, China) was employed for antigen recognition, and a secondary anti-mouse antibody (Abmart, Shanghai, China) was used as recommended for signal amplification. ..



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    Image Search Results


    Journal: iScience

    Article Title: Pentachlorophenol enhances bladder cancer cell invasion through altered protein stability

    doi: 10.1016/j.isci.2026.117357

    Figure Lengend Snippet:

    Article Snippet: HRP-conjugated secondary antibody , ABclonal , Cat# AS014; RRID: AB_2863475.

    Techniques: Recombinant, CCK-8 Assay, Bicinchoninic Acid Protein Assay, shRNA, Plasmid Preparation, Over Expression, Software, Transfection, Western Blot