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rabbit anti lc3  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti lc3
    Rabbit Anti Lc3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/LC3B+Antibody+-+BSA+Free/pm41912478-90-103-106
    Average 96 stars, based on 1654 article reviews
    rabbit anti lc3 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice.
    Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100- 2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti- Parkin antibody (1:500, ab77924, Abcam), anti- VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice
    Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100‐2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti‐Parkin antibody (1:500, ab77924, Abcam), anti‐VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Incubation:

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice.
    Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100- 2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti- Parkin antibody (1:500, ab77924, Abcam), anti- VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice
    Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100‐2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti‐Parkin antibody (1:500, ab77924, Abcam), anti‐VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Western Blot:

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice.
    Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100- 2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti- Parkin antibody (1:500, ab77924, Abcam), anti- VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice
    Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100‐2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti‐Parkin antibody (1:500, ab77924, Abcam), anti‐VDAC1 (1:1000, ab14734, Abcam), and Cytochrome c Antibody (1:1000, 4272, Cell Signaling). ..

    Article Title: Targeting VPS4 elicits STING-driven anti-tumor immunity to suppress rhabdomyosarcoma growth.
    Article Snippet: .. The following antibodies were used for immunoblotting: GFP (Cell Signaling Technology [Danvers, MA, USA], #2956S, 1:1000), IRF3 (Cell Signaling Technology #4302S, 1:1000), phospho-TBK1 (Cell Signaling Technology #5483S, 1:1000), TBK1 (Cell Signaling Technology #71543SF, 1:1000), phospho-STING (Cell Signaling Technology #72971S, 1:1000), STING (Cell Signaling Technology #13647S, 1:1000), Cleaved caspase 3 (Cell Signaling Technology #9661S, 1:1000), β-actin (Sigma-Aldrich [St. Louis, MI, USA], #A5441, 1:10,000), VPS4A (Sigma-Aldrich #SAB4200215, 1:1000), VPS4B (Santa Cruz Biotechnology [Dallas, TX, USA], #sc-377162, 1:200), cGAS (Cell Signaling Technology #31659S, 1:1000), p62/SQSTM1 (PROGEN Biotechnik [Heidelberg, Germany], #GP62-C, 1:1000), ATF-3 (Cell Signaling Technology #18665S, 1:1000), and LC3B (Novus Biologicals [Centennial, CO, USA] #NB100-2220, 1:5000). .. The following inhibitors were used to dissect innate immune signaling pathways: BMS-345541 (Sigma-Aldrich #B9935-5MG), 5Z-7-oxozeaenol (MedChemExpress [Monmouth Junction, NJ, USA], #HY-12686), GSK8612 (MedChemExpress #HY-111941), C-176 (MedChemExpress #HY-112906), NBP2-31226 (Novus Biologicals #NBP2-31226), RIG012 (MedChemExpress #HY-147124), and MSA-2 (MedChemExpress #HY-136927).

    Control:

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Nucleic Acid Electrophoresis:

    Article Title: Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulation
    Article Snippet: .. Proteins (20 μg per lane) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, and probed overnight at 4 °C with the following primary antibodies: anti-LC3B (microtubule-associated protein 1 light chain 3 beta; NOVUS, NB100-2220), anti-cleaved caspase-3 (CST, 9662) (1:1000), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Proteintech, 60004-1-Ig). ..

    Membrane:

    Article Title: Agrimol B inhibits pancreatic ductal adenocarcinoma by induction of lethal mitophagy through decreasing mitochondrial transcription termination factor 3
    Article Snippet: Primary antibodies against caspase 9 (680095), HADHA ( R24526 ), superoxide dismutase 2 (SOD2) (306 028), autophagy related 7 (ATG7) ( R23498 ), Sequestosome-1 (P62) (380612), cathepsin D (CTSD) (380946), and voltage dependent anion channel 1 (VDAC1) ( R26067 ) were acquired from Zenbio. .. An anti-microtubule-associated protein light chain 3 (LC3) antibody (NB100-2220) was purchased from Novus, while anti-MTERF3 (EM1701-29) and lysosomal associated membrane protein 2 (LAMP2) (M1603-5) antibodies were purchased from HuaBio. .. The anti-Parkin (14060–1-AP) and mitochondrial import inner membrane translocase subunit 23 (TIM23) (11123–1-AP) antibodies were purchased from Proteintech, and an anti-PINK1 antibody (ab216144) was purchased from Abcam.



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