Review



rabbit anti lc3b  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Novus Biologicals rabbit anti lc3b
    Rabbit Anti Lc3b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/LC3B+Antibody+-+BSA+Free/bio_rxiv__64898__2026__04__21__719846-157-12-15
    Average 96 stars, based on 1671 article reviews
    rabbit anti lc3b - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice.
    Article Snippet: Kaleidoscope or Precision Plus Protein Standards (1610375 and 1610374, Bio- Rad) were loaded at both ends of the membrane for reference.

    Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice
    Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight.

    Incubation:

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Control:

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Nucleic Acid Electrophoresis:

    Article Title: Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulation
    Article Snippet: .. Proteins (20 μg per lane) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, and probed overnight at 4 °C with the following primary antibodies: anti-LC3B (microtubule-associated protein 1 light chain 3 beta; NOVUS, NB100-2220), anti-cleaved caspase-3 (CST, 9662) (1:1000), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Proteintech, 60004-1-Ig). ..

    Membrane:

    Article Title: Agrimol B inhibits pancreatic ductal adenocarcinoma by induction of lethal mitophagy through decreasing mitochondrial transcription termination factor 3
    Article Snippet: Primary antibodies against caspase 9 (680095), HADHA ( R24526 ), superoxide dismutase 2 (SOD2) (306 028), autophagy related 7 (ATG7) ( R23498 ), Sequestosome-1 (P62) (380612), cathepsin D (CTSD) (380946), and voltage dependent anion channel 1 (VDAC1) ( R26067 ) were acquired from Zenbio. .. An anti-microtubule-associated protein light chain 3 (LC3) antibody (NB100-2220) was purchased from Novus, while anti-MTERF3 (EM1701-29) and lysosomal associated membrane protein 2 (LAMP2) (M1603-5) antibodies were purchased from HuaBio. .. The anti-Parkin (14060–1-AP) and mitochondrial import inner membrane translocase subunit 23 (TIM23) (11123–1-AP) antibodies were purchased from Proteintech, and an anti-PINK1 antibody (ab216144) was purchased from Abcam.

    Western Blot:

    Article Title: Targeting VPS4 elicits STING-driven anti-tumor immunity to suppress rhabdomyosarcoma growth.
    Article Snippet: .. The following antibodies were used for immunoblotting: GFP (Cell Signaling Technology [Danvers, MA, USA], #2956S, 1:1000), IRF3 (Cell Signaling Technology #4302S, 1:1000), phospho-TBK1 (Cell Signaling Technology #5483S, 1:1000), TBK1 (Cell Signaling Technology #71543SF, 1:1000), phospho-STING (Cell Signaling Technology #72971S, 1:1000), STING (Cell Signaling Technology #13647S, 1:1000), Cleaved caspase 3 (Cell Signaling Technology #9661S, 1:1000), β-actin (Sigma-Aldrich [St. Louis, MI, USA], #A5441, 1:10,000), VPS4A (Sigma-Aldrich #SAB4200215, 1:1000), VPS4B (Santa Cruz Biotechnology [Dallas, TX, USA], #sc-377162, 1:200), cGAS (Cell Signaling Technology #31659S, 1:1000), p62/SQSTM1 (PROGEN Biotechnik [Heidelberg, Germany], #GP62-C, 1:1000), ATF-3 (Cell Signaling Technology #18665S, 1:1000), and LC3B (Novus Biologicals [Centennial, CO, USA] #NB100-2220, 1:5000). .. The following inhibitors were used to dissect innate immune signaling pathways: BMS-345541 (Sigma-Aldrich #B9935-5MG), 5Z-7-oxozeaenol (MedChemExpress [Monmouth Junction, NJ, USA], #HY-12686), GSK8612 (MedChemExpress #HY-111941), C-176 (MedChemExpress #HY-112906), NBP2-31226 (Novus Biologicals #NBP2-31226), RIG012 (MedChemExpress #HY-147124), and MSA-2 (MedChemExpress #HY-136927).

    Cell-Signaling:

    Article Title: Targeting VPS4 elicits STING-driven anti-tumor immunity to suppress rhabdomyosarcoma growth.
    Article Snippet: .. The following antibodies were used for immunoblotting: GFP (Cell Signaling Technology [Danvers, MA, USA], #2956S, 1:1000), IRF3 (Cell Signaling Technology #4302S, 1:1000), phospho-TBK1 (Cell Signaling Technology #5483S, 1:1000), TBK1 (Cell Signaling Technology #71543SF, 1:1000), phospho-STING (Cell Signaling Technology #72971S, 1:1000), STING (Cell Signaling Technology #13647S, 1:1000), Cleaved caspase 3 (Cell Signaling Technology #9661S, 1:1000), β-actin (Sigma-Aldrich [St. Louis, MI, USA], #A5441, 1:10,000), VPS4A (Sigma-Aldrich #SAB4200215, 1:1000), VPS4B (Santa Cruz Biotechnology [Dallas, TX, USA], #sc-377162, 1:200), cGAS (Cell Signaling Technology #31659S, 1:1000), p62/SQSTM1 (PROGEN Biotechnik [Heidelberg, Germany], #GP62-C, 1:1000), ATF-3 (Cell Signaling Technology #18665S, 1:1000), and LC3B (Novus Biologicals [Centennial, CO, USA] #NB100-2220, 1:5000). .. The following inhibitors were used to dissect innate immune signaling pathways: BMS-345541 (Sigma-Aldrich #B9935-5MG), 5Z-7-oxozeaenol (MedChemExpress [Monmouth Junction, NJ, USA], #HY-12686), GSK8612 (MedChemExpress #HY-111941), C-176 (MedChemExpress #HY-112906), NBP2-31226 (Novus Biologicals #NBP2-31226), RIG012 (MedChemExpress #HY-147124), and MSA-2 (MedChemExpress #HY-136927).



    Similar Products

    98
    ABclonal Biotechnology anti lc3b
    Anti Lc3b, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/%5BKO+Validated%5D+LC3B+Rabbit+mAb/10__2147_slash_ijn__s618525-135-7-8
    Average 98 stars, based on 1 article reviews
    anti lc3b - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology lc3b rabbit mab
    Antibodies used in this study.
    Lc3b Rabbit Mab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/%5BKO+Validated%5D+LC3B+Rabbit+mAb/pmc13615372-7-0-4
    Average 98 stars, based on 1 article reviews
    lc3b rabbit mab - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology anti lc3b antibody
    Antibodies used in this study.
    Anti Lc3b Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/%5BKO+Validated%5D+LC3B+Rabbit+mAb/pm42778580-130-50-55
    Average 98 stars, based on 1 article reviews
    anti lc3b antibody - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology lc3b
    Antibodies used in this study.
    Lc3b, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/%5BKO+Validated%5D+LC3B+Rabbit+mAb/pmc13604869-86-30-33
    Average 98 stars, based on 1 article reviews
    lc3b - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology rabbit anti lc3b
    Antibodies used in this study.
    Rabbit Anti Lc3b, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/%5BKO+Validated%5D+LC3B+Rabbit+mAb/pm42711381-230-96-101
    Average 98 stars, based on 1 article reviews
    rabbit anti lc3b - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology lc3a lc3b
    Antibodies used in this study.
    Lc3a Lc3b, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3b/LC3A%2FLC3B+Rabbit+pAb/10__1016_slash_j__apsb__2026__09__023-90-33-35
    Average 95 stars, based on 1 article reviews
    lc3a lc3b - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    Antibodies used in this study.

    Journal: Pediatric Discovery

    Article Title: Early Growth Response 1‐Associated Epithelial Autophagy‐Related Changes in Allergic Airway Inflammation

    doi: 10.1002/pdi3.70075

    Figure Lengend Snippet: Antibodies used in this study.

    Article Snippet: LC3B rabbit mAb , ABclonal , A19665 , ARC0144.

    Techniques: Cell-Signaling

    House dust mite (HDM) stimulation induces early growth response 1 (EGR1) expression and autophagy‐associated changes in airway epithelial cells. (A–C) EGR1 mRNA and protein levels in BEAS‐2B cells stimulated with HDM extract (30 μg/mL, 3 hours) or vehicle control, assessed by quantitative polymerase chain reaction (qPCR) and Western blot. (D–F) Western blot analysis of LC3B and p62 protein levels. The LC3B‐II/I ratio was quantified by densitometry. (G) Representative immunofluorescence images of LC3B puncta (green) in BEAS‐2B cells. Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. (H) IL‐33 release into culture supernatants measured by ELISA. (I) Airway resistance in response to increasing concentrations of methacholine in HDM‐challenged mice and controls. Data were analyzed using repeated‐measures ANOVA. (J) Representative hematoxylin and eosin‐stained lung tissue sections. Scale bar, 100 μm. (K–M) Immunofluorescence detection of EGR1 (red) and LC3B (green) in lung tissue sections. Nuclei were stained with DAPI (blue). Fluorescence intensity was quantified and normalized to the control. Data are presented as mean ± standard deviation. For in vitro experiments: (A, G) n = 3 independent experiments; (B–F) n = 4 independent experiments. For in vivo experiments: n = 4 mice per group. Statistical significance was determined by an unpaired Student's t ‐test unless otherwise indicated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Journal: Pediatric Discovery

    Article Title: Early Growth Response 1‐Associated Epithelial Autophagy‐Related Changes in Allergic Airway Inflammation

    doi: 10.1002/pdi3.70075

    Figure Lengend Snippet: House dust mite (HDM) stimulation induces early growth response 1 (EGR1) expression and autophagy‐associated changes in airway epithelial cells. (A–C) EGR1 mRNA and protein levels in BEAS‐2B cells stimulated with HDM extract (30 μg/mL, 3 hours) or vehicle control, assessed by quantitative polymerase chain reaction (qPCR) and Western blot. (D–F) Western blot analysis of LC3B and p62 protein levels. The LC3B‐II/I ratio was quantified by densitometry. (G) Representative immunofluorescence images of LC3B puncta (green) in BEAS‐2B cells. Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. (H) IL‐33 release into culture supernatants measured by ELISA. (I) Airway resistance in response to increasing concentrations of methacholine in HDM‐challenged mice and controls. Data were analyzed using repeated‐measures ANOVA. (J) Representative hematoxylin and eosin‐stained lung tissue sections. Scale bar, 100 μm. (K–M) Immunofluorescence detection of EGR1 (red) and LC3B (green) in lung tissue sections. Nuclei were stained with DAPI (blue). Fluorescence intensity was quantified and normalized to the control. Data are presented as mean ± standard deviation. For in vitro experiments: (A, G) n = 3 independent experiments; (B–F) n = 4 independent experiments. For in vivo experiments: n = 4 mice per group. Statistical significance was determined by an unpaired Student's t ‐test unless otherwise indicated. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Article Snippet: LC3B rabbit mAb , ABclonal , A19665 , ARC0144.

    Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Western Blot, Analysis, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Staining, Tissue, Fluorescence, Standard Deviation, In Vitro, In Vivo

    Validation of early growth indicaton 1 ( EGR1 ) overexpression and knockdown efficiency, and the effects on autophagy‐related proteins and IL‐33 release in house dust mite (HDM)‐stimulated BEAS‐2B cells. (A–C) EGR1 mRNA and EGR1 protein levels in cells transfected with the EGR1 overexpression plasmid (oe‐EGR1) or empty vector, assessed by quantitative polymerase chain reaction (qPCR) and Western blot. (D–F) EGR1 mRNA and ERG1 protein levels in cells transfected with EGR1‐targeting siRNA (si‐EGR1) or scrambled control, assessed by qPCR and Western blot. (G–I) Western blot analysis of LC3B and p62 in oe‐EGR1‐transfected cells, followed by HDM stimulation (30 μg/mL, 3 h). The LC3B‐II/I ratio and p62 levels were quantified by densitometry. (J) IL‐33 release into culture supernatants under the same conditions, measured by ELISA. (K–M) Western blot analysis of LC3B and p62 in si‐EGR1‐transfected cells with HDM stimulation. (N) Corresponding IL‐33 release measured by ELISA. (O–P) Immunofluorescence detection of LC3B puncta (green) in oe‐EGR1 and si‐EGR1 cells stimulated with HDM. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. All data are presented as mean ± standard deviation ( n = 3 independent experiments). Statistical significance for panels (A–P) was determined by one‐way ANOVA followed by appropriate post‐hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Journal: Pediatric Discovery

    Article Title: Early Growth Response 1‐Associated Epithelial Autophagy‐Related Changes in Allergic Airway Inflammation

    doi: 10.1002/pdi3.70075

    Figure Lengend Snippet: Validation of early growth indicaton 1 ( EGR1 ) overexpression and knockdown efficiency, and the effects on autophagy‐related proteins and IL‐33 release in house dust mite (HDM)‐stimulated BEAS‐2B cells. (A–C) EGR1 mRNA and EGR1 protein levels in cells transfected with the EGR1 overexpression plasmid (oe‐EGR1) or empty vector, assessed by quantitative polymerase chain reaction (qPCR) and Western blot. (D–F) EGR1 mRNA and ERG1 protein levels in cells transfected with EGR1‐targeting siRNA (si‐EGR1) or scrambled control, assessed by qPCR and Western blot. (G–I) Western blot analysis of LC3B and p62 in oe‐EGR1‐transfected cells, followed by HDM stimulation (30 μg/mL, 3 h). The LC3B‐II/I ratio and p62 levels were quantified by densitometry. (J) IL‐33 release into culture supernatants under the same conditions, measured by ELISA. (K–M) Western blot analysis of LC3B and p62 in si‐EGR1‐transfected cells with HDM stimulation. (N) Corresponding IL‐33 release measured by ELISA. (O–P) Immunofluorescence detection of LC3B puncta (green) in oe‐EGR1 and si‐EGR1 cells stimulated with HDM. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. All data are presented as mean ± standard deviation ( n = 3 independent experiments). Statistical significance for panels (A–P) was determined by one‐way ANOVA followed by appropriate post‐hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Article Snippet: LC3B rabbit mAb , ABclonal , A19665 , ARC0144.

    Techniques: Biomarker Discovery, Over Expression, Knockdown, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Western Blot, Control, Analysis, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Standard Deviation

    PI3K‐III (Vps34) protein levels in response to house dust mite (HDM) and early growth response 1 (EGR1) manipulation, and the impact of 3‐methyladenine (3‐MA) on autophagy and IL‐33 release in EGR1‐overexpressing cells. (A–B) Western blot analysis and quantification of Vps34 protein levels in BEAS‐2B cells stimulated with HDM extract (30 μg/mL, 3 h) or vehicle control. (C–D) Western blot analysis of Vps34 protein levels in cells transfected with oe‐EGR1 or empty vector, with or without HDM stimulation. (E–F) Western blot analysis of Vps34 protein levels in cells transfected with si‐EGR1 or scrambled control, with or without HDM stimulation. (G–I) Western blot analysis of LC3B and p62 in oe‐EGR1‐transfected cells treated with or without Class III PI3K inhibitor 3‐methyladenine (3‐MA, 10 mmol/L, 6 h). (J) IL‐33 release in the same experimental setting, measured by ELISA. All data are presented as mean ± standard deviation ( n = 3 independent experiments). For panels (A–B), statistical significance was determined by an unpaired Student's t ‐test. For panels (C–J), one‐way ANOVA followed by appropriate post‐hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Journal: Pediatric Discovery

    Article Title: Early Growth Response 1‐Associated Epithelial Autophagy‐Related Changes in Allergic Airway Inflammation

    doi: 10.1002/pdi3.70075

    Figure Lengend Snippet: PI3K‐III (Vps34) protein levels in response to house dust mite (HDM) and early growth response 1 (EGR1) manipulation, and the impact of 3‐methyladenine (3‐MA) on autophagy and IL‐33 release in EGR1‐overexpressing cells. (A–B) Western blot analysis and quantification of Vps34 protein levels in BEAS‐2B cells stimulated with HDM extract (30 μg/mL, 3 h) or vehicle control. (C–D) Western blot analysis of Vps34 protein levels in cells transfected with oe‐EGR1 or empty vector, with or without HDM stimulation. (E–F) Western blot analysis of Vps34 protein levels in cells transfected with si‐EGR1 or scrambled control, with or without HDM stimulation. (G–I) Western blot analysis of LC3B and p62 in oe‐EGR1‐transfected cells treated with or without Class III PI3K inhibitor 3‐methyladenine (3‐MA, 10 mmol/L, 6 h). (J) IL‐33 release in the same experimental setting, measured by ELISA. All data are presented as mean ± standard deviation ( n = 3 independent experiments). For panels (A–B), statistical significance was determined by an unpaired Student's t ‐test. For panels (C–J), one‐way ANOVA followed by appropriate post‐hoc tests was used. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns, not significant.

    Article Snippet: LC3B rabbit mAb , ABclonal , A19665 , ARC0144.

    Techniques: Western Blot, Analysis, Control, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Standard Deviation