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GenScript corporation quickblocktm blocking buffer
Quickblocktm Blocking Buffer, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblocktm+western+blocking+buffer/quickblocktm+blocking+buffer/pm37371903-108-6-9
Average 90 stars, based on 1 article reviews
quickblocktm blocking buffer - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Exploring the pathophysiology underlying clozapine-induced enhancement of glutamatergic transmission through L-glutamate and D-serine release associated with pannexin1 hemichannels.
Article Snippet: Next, the capillaries were incubated with the blocking reagent for 15 min, and the target proteins were probed with primary antibodies, followed by incubation with HRP-conjugated secondary antibodies (Anti-Rabbit IgG HRP, A00098, RRID:AB_1968815, 10 μg ml 1; GenScript, Piscataway, NJ, USA).

Article Title: Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats.
Article Snippet: Next, the capillaries were incubated with a blocking reagent for 15 min and the target proteins were probed with primary antibodies, followed by incubation with HRP-conjugated secondary antibodies (anti-rabbit HRP-conjugated IgG, A00098, 10 μg/mL, GenScript, Piscataway, NJ, USA).

Article Title: Melatonin Alleviates Retinal Ischemia–Reperfusion Injury by Inhibiting p53–Mediated Ferroptosis
Article Snippet: After blocking for 10 min with QuickBlockTM Blocking Buffer (Genscript, Nanjing, China), the membranes were incubated with primary antibodies for 4 °C overnight followed by incubation with HRP–conjugated secondary antibodies for 2 h at room temperature.

Membrane:

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression.
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17 min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40 min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Blocking Assay:

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression.
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Article Title: IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy
Article Snippet: Protein lysates were separated on 12% polyacrylamide gels and electrotransferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Darmstadt, Germany). .. Membranes were blocked with QuickBlockTM Blocking Buffer (Genscript, Nanjing, China) for 20 minute and then incubated overnight with primary antibodies against α-SMA, COL1A1, ERK-, Phospho-ERK (p-ERK), STAT3, and phospho-STAT3 (p-STAT3). ..

Article Title: Melatonin Alleviates Retinal Ischemia-Reperfusion Injury by Inhibiting p53-Mediated Ferroptosis.
Article Snippet: The retinal protein concentration was determined using bicinchoninic acid (BCA) kit (KeyGen BioTECH), and equal amounts of total protein were electrophoresed on 10% polyacrylamide gel and subsequently transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA) following a standard protocol. .. After blocking for 10 min with QuickBlockTM Blocking Buffer (Genscript, Nanjing, China), the membranes were incubated with primary antibodies for 4 ◦C overnight followed by incubation with HRP–conjugated secondary antibodies for 2 h at room temperature. .. After washing three times with 0.1% Tween–20/TBS, the immunoreactive proteins were visualized with an enhanced chemiluminescence kit (Millipore) and a chemiluminescence imager (Tanon Science & Technology, Shanghai, China).

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17 min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40 min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Article Title: SOX9 Induces Orbital Fibroblast Activation in Thyroid Eye Disease Via MAPK/ERK1/2 Pathway
Article Snippet: Equal amounts of total protein were separated on sodium dodecyl sulfate–polyacrylamide gels (8% or 12%) and electrotransferred onto polyvinylidene fluoride membranes (Millipore, Darmstadt, Germany). .. To block any nonspecific binding, the membranes were incubated in QuickBlock Blocking Buffer (Genscript, Nanjing, China) for 15 minutes and subsequently incubated overnight with primary antibodies against α-smooth muscle actin (α-SMA), collagen type I alpha 1 (COL1α1), connective tissue growth factor (CTGF), epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK)1/2, p-ERK1/2, c-Jun N-terminal kinase (JNK), p-JNK, P38, p-P38 (all from CST, Beverly, MA, USA) and SOX9 (Abcam, Cambridge, MA, USA). ..

Incubation:

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression.
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Article Title: IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy
Article Snippet: Protein lysates were separated on 12% polyacrylamide gels and electrotransferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Darmstadt, Germany). .. Membranes were blocked with QuickBlockTM Blocking Buffer (Genscript, Nanjing, China) for 20 minute and then incubated overnight with primary antibodies against α-SMA, COL1A1, ERK-, Phospho-ERK (p-ERK), STAT3, and phospho-STAT3 (p-STAT3). ..

Article Title: Melatonin Alleviates Retinal Ischemia-Reperfusion Injury by Inhibiting p53-Mediated Ferroptosis.
Article Snippet: The retinal protein concentration was determined using bicinchoninic acid (BCA) kit (KeyGen BioTECH), and equal amounts of total protein were electrophoresed on 10% polyacrylamide gel and subsequently transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA) following a standard protocol. .. After blocking for 10 min with QuickBlockTM Blocking Buffer (Genscript, Nanjing, China), the membranes were incubated with primary antibodies for 4 ◦C overnight followed by incubation with HRP–conjugated secondary antibodies for 2 h at room temperature. .. After washing three times with 0.1% Tween–20/TBS, the immunoreactive proteins were visualized with an enhanced chemiluminescence kit (Millipore) and a chemiluminescence imager (Tanon Science & Technology, Shanghai, China).

Article Title: Circular RNA hsa_circ_0007367 promotes the progression of pancreatic ductal adenocarcinoma by sponging miR-6820-3p and upregulating YAP1 expression
Article Snippet: After the cell lysates were sonicated, and treated with the BCA Protein Analysis kit (Beyotime, China) to detect the concentration of protein by using an enzyme-labeled instrument (Molecular Devices, USA). .. Proteins were used to SDS-page and transferred to nitrocellulose filter membrane (Pall Corporation, USA), blocked with rapid blocking buffer (GenScript, USA) for 17 min and incubated with primary antibody overnight at 4 °C. .. The next day it was washed with TBST and incubated with secondary antibodies for 40 min, the membranes were scanned and analyzed with Odyssey infrared imaging instrument (LI-COR, USA).

Article Title: SOX9 Induces Orbital Fibroblast Activation in Thyroid Eye Disease Via MAPK/ERK1/2 Pathway
Article Snippet: Equal amounts of total protein were separated on sodium dodecyl sulfate–polyacrylamide gels (8% or 12%) and electrotransferred onto polyvinylidene fluoride membranes (Millipore, Darmstadt, Germany). .. To block any nonspecific binding, the membranes were incubated in QuickBlock Blocking Buffer (Genscript, Nanjing, China) for 15 minutes and subsequently incubated overnight with primary antibodies against α-smooth muscle actin (α-SMA), collagen type I alpha 1 (COL1α1), connective tissue growth factor (CTGF), epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK)1/2, p-ERK1/2, c-Jun N-terminal kinase (JNK), p-JNK, P38, p-P38 (all from CST, Beverly, MA, USA) and SOX9 (Abcam, Cambridge, MA, USA). ..

Binding Assay:

Article Title: SOX9 Induces Orbital Fibroblast Activation in Thyroid Eye Disease Via MAPK/ERK1/2 Pathway
Article Snippet: Equal amounts of total protein were separated on sodium dodecyl sulfate–polyacrylamide gels (8% or 12%) and electrotransferred onto polyvinylidene fluoride membranes (Millipore, Darmstadt, Germany). .. To block any nonspecific binding, the membranes were incubated in QuickBlock Blocking Buffer (Genscript, Nanjing, China) for 15 minutes and subsequently incubated overnight with primary antibodies against α-smooth muscle actin (α-SMA), collagen type I alpha 1 (COL1α1), connective tissue growth factor (CTGF), epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK)1/2, p-ERK1/2, c-Jun N-terminal kinase (JNK), p-JNK, P38, p-P38 (all from CST, Beverly, MA, USA) and SOX9 (Abcam, Cambridge, MA, USA). ..



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