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Laborclin BR diff-quick
Diff Quick, supplied by Laborclin BR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quick-diff/diff+quick/pm24760021-49-23-24
Average 90 stars, based on 1 article reviews
diff-quick - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Staining:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.

Diff-Quik:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.

Incubation:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.

Microscopy:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.

Histopathology:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.

Sterility:

Article Title: Functional Study of Leishmania braziliensis Protein Arginine Methyltransferases (PRMTs) Reveals That PRMT1 and PRMT5 Are Required for Macrophage Infection.
Article Snippet: The cells were incubated in fresh complete RPMI for 24 and 72 h. Coverslips were collected at each time point, stained using the Diff-Quick method (LaborClin, Brazil) and mounted onto glass slides.

Article Title: HSP90 inhibitor gedunin impairs glioblastoma proliferation and invasiveness
Article Snippet: The chamber was incubated in a humidified atmosphere with 5 % CO2 at 37 °C for 24 h. The membranes were removed and stained using a Diff-quick (Laborclin, Brazil).

Article Title: Ovarian and Oocyte Morphology During the Spring-Summer and Fall-Winter in Queens (Felis catus) Kept in a Tropical Climate.
Article Snippet: The objective of this study was to investigate the effects of seasonality and phase of the estrous cycle on ovarian and oocyte morphology in domestic cats kept in a tropical region.. The ovaries from 23 queens were collected after elective ovariohysterectomy at different stages of the estrous cycle and were classified according to the season upon collection (spring/summer vs. fall/winter).. Based on the findings, we concluded that queens in tropical regions experience no seasonal influence on oocyte quality, but there is a minimal effect on ovarian morphology, with an increase in ovarian length during proestrus/estrus in the spring/summer period.

Article Title: Perfil da microbiota fecal de gatas ovariohisterectomizadas submetidas à reposição estrogênica
Article Snippet: Vaginal smear slides were stained with Diff-Quick (Laborclin, Pinhais, Brazil) and examined at 40x magnification to provide interpretations regarding the hormonal phase and estrogenic stimulus.

Article Title: Richardia brasiliensis Gomes: phytochemical characterization, antiproliferative capacity and in vitro and in vivo toxicity.
Article Snippet: Richardia brasiliensis, known as poaia branca, is a medicinal species widely distributed throughout Brazil and used in folk medicine.. However, studies on its toxicity are practically non-existent, and little is known about its biological activity.. This study aimed to investigate its phytochemical compounds, assess its in vitro and in vivo toxicities, and determine its antiproliferative activity.

Article Title: Comparison of Diff-Quick and Spermac Staining Methods for Sperm Morphology Evaluation
Article Snippet: To assess the sperm morphology, liquefied semen was mixed and dried smears were stained using either the Diff-Quick (Panótico Rápido® kit; Laborclin, Brazil) or Spermac (Spermac stain®; FertiPro N.V., Belgium) method.

Article Title: Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?
Article Snippet: Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response.. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen.. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI.

Article Title: Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis.
Article Snippet: The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage.. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A.. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses.



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction