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ptat2 2 expression vector  (Addgene inc)


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    Addgene inc ptat2 2 expression vector
    Ptat2 2 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptat2+2+expression+vector/pTAT2%2E2+(Plasmid+%2335621)/pmc05056240-68-22-25
    Average 93 stars, based on 1 article reviews
    ptat2 2 expression vector - by Bioz Stars, 2026-09
    93/100 stars

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    Purification:

    Article Title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress
    Article Snippet: The bcp1 coding sequence was isolated from the pET30Bcp1 vector [ ] after digestion with the Nde I and Xho I restriction endonucleases. .. The bcp1 fragment was purified by agarose gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen, Milano, Italy) and cloned into the pTAT2.2 expression vector (Addgene, Cambridge, USA). .. E. coli BL21-CodonPlus (DE3)RIL competent cells were transformed with the obtained vector, pTAT2.2-Bcp1, and selected on LB agar plates with kanamycin (10 μ g mL −1 ) and chloramphenicol (34 μ g mL −1 ).

    Agarose Gel Electrophoresis:

    Article Title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress
    Article Snippet: The bcp1 coding sequence was isolated from the pET30Bcp1 vector [ ] after digestion with the Nde I and Xho I restriction endonucleases. .. The bcp1 fragment was purified by agarose gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen, Milano, Italy) and cloned into the pTAT2.2 expression vector (Addgene, Cambridge, USA). .. E. coli BL21-CodonPlus (DE3)RIL competent cells were transformed with the obtained vector, pTAT2.2-Bcp1, and selected on LB agar plates with kanamycin (10 μ g mL −1 ) and chloramphenicol (34 μ g mL −1 ).

    Gel Extraction:

    Article Title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress
    Article Snippet: The bcp1 coding sequence was isolated from the pET30Bcp1 vector [ ] after digestion with the Nde I and Xho I restriction endonucleases. .. The bcp1 fragment was purified by agarose gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen, Milano, Italy) and cloned into the pTAT2.2 expression vector (Addgene, Cambridge, USA). .. E. coli BL21-CodonPlus (DE3)RIL competent cells were transformed with the obtained vector, pTAT2.2-Bcp1, and selected on LB agar plates with kanamycin (10 μ g mL −1 ) and chloramphenicol (34 μ g mL −1 ).

    Clone Assay:

    Article Title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress
    Article Snippet: The bcp1 coding sequence was isolated from the pET30Bcp1 vector [ ] after digestion with the Nde I and Xho I restriction endonucleases. .. The bcp1 fragment was purified by agarose gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen, Milano, Italy) and cloned into the pTAT2.2 expression vector (Addgene, Cambridge, USA). .. E. coli BL21-CodonPlus (DE3)RIL competent cells were transformed with the obtained vector, pTAT2.2-Bcp1, and selected on LB agar plates with kanamycin (10 μ g mL −1 ) and chloramphenicol (34 μ g mL −1 ).

    Expressing:

    Article Title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress
    Article Snippet: The bcp1 coding sequence was isolated from the pET30Bcp1 vector [ ] after digestion with the Nde I and Xho I restriction endonucleases. .. The bcp1 fragment was purified by agarose gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen, Milano, Italy) and cloned into the pTAT2.2 expression vector (Addgene, Cambridge, USA). .. E. coli BL21-CodonPlus (DE3)RIL competent cells were transformed with the obtained vector, pTAT2.2-Bcp1, and selected on LB agar plates with kanamycin (10 μ g mL −1 ) and chloramphenicol (34 μ g mL −1 ).



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    ptat2 2 expression vector  (Addgene inc)
    93
    Addgene inc ptat2 2 expression vector
    Ptat2 2 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptat2+2+expression+vector/pTAT2%2E2+(Plasmid+%2335621)/pmc05056240-68-22-25
    Average 93 stars, based on 1 article reviews
    ptat2 2 expression vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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