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prmt5  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology prmt5
    A . Immunoprecipitation (IP) with the indicated antibodies from HEK293 whole cell lysate (WCL), followed by western blotting with the indicated antibodies. IP with IgG was used as a negative control. The blots indicate that POLR2A, SMN, FUS, TDP-43, <t>PRMT5</t> and the termination factors SETX and XRN2 interact directly or indirectly with each other . B . A summary of the interactions detected by co-IP experiments. All of these interactions, except the SMN-XRN2 and SMN-TDP-43 interactions, have been shown by various published experiments to be direct ( , , ).
    Prmt5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prmt5+knockdown+cells/bio_rxiv__788778-103-22-26?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 115 article reviews
    prmt5 - by Bioz Stars, 2026-08
    93/100 stars

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    1) Product Images from "Regulation of transcription termination by FUS and TDP-43"

    Article Title: Regulation of transcription termination by FUS and TDP-43

    Journal: bioRxiv

    doi: 10.1101/788778

    A . Immunoprecipitation (IP) with the indicated antibodies from HEK293 whole cell lysate (WCL), followed by western blotting with the indicated antibodies. IP with IgG was used as a negative control. The blots indicate that POLR2A, SMN, FUS, TDP-43, PRMT5 and the termination factors SETX and XRN2 interact directly or indirectly with each other . B . A summary of the interactions detected by co-IP experiments. All of these interactions, except the SMN-XRN2 and SMN-TDP-43 interactions, have been shown by various published experiments to be direct ( , , ).
    Figure Legend Snippet: A . Immunoprecipitation (IP) with the indicated antibodies from HEK293 whole cell lysate (WCL), followed by western blotting with the indicated antibodies. IP with IgG was used as a negative control. The blots indicate that POLR2A, SMN, FUS, TDP-43, PRMT5 and the termination factors SETX and XRN2 interact directly or indirectly with each other . B . A summary of the interactions detected by co-IP experiments. All of these interactions, except the SMN-XRN2 and SMN-TDP-43 interactions, have been shown by various published experiments to be direct ( , , ).

    Techniques Used: Immunoprecipitation, Western Blot, Negative Control, Co-Immunoprecipitation Assay

    A . IP with 8WG16 antibodies for POLR2A from HEK293 WCL upon stably knocking down PRMT5 or SMN, with the knock-down of GFP as negative control. Western blots were performed with the indicated antibodies. The knock-down of PRMT5 or SMN caused a reduction of FUS and TDP-43 interaction with RNAPII. B . IP with the indicated antibodies from HEK293 WCL, upon knocking out of SMN with the CRISPR/Cas9 system or using scrambled guide RNA as negative control. Western blots were performed with the indicated antibodies to show that the SMN knock-out leads to a loss of interaction of TDP-43 and SETX with POLR2A. IP with IgG as negative control. C, D . Quantifications of ChIP in HEK293 cells as FUS/POLR2A ( C ) or TDP-43/POLR2A ( D ) ratio to show the relative effects of knocking down PRMT5 or SMN. Error bars denote biological replicate s.e.m. (n=3).
    Figure Legend Snippet: A . IP with 8WG16 antibodies for POLR2A from HEK293 WCL upon stably knocking down PRMT5 or SMN, with the knock-down of GFP as negative control. Western blots were performed with the indicated antibodies. The knock-down of PRMT5 or SMN caused a reduction of FUS and TDP-43 interaction with RNAPII. B . IP with the indicated antibodies from HEK293 WCL, upon knocking out of SMN with the CRISPR/Cas9 system or using scrambled guide RNA as negative control. Western blots were performed with the indicated antibodies to show that the SMN knock-out leads to a loss of interaction of TDP-43 and SETX with POLR2A. IP with IgG as negative control. C, D . Quantifications of ChIP in HEK293 cells as FUS/POLR2A ( C ) or TDP-43/POLR2A ( D ) ratio to show the relative effects of knocking down PRMT5 or SMN. Error bars denote biological replicate s.e.m. (n=3).

    Techniques Used: Stable Transfection, Knockdown, Negative Control, Western Blot, CRISPR, Knock-Out



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