primescript 1st strand cdna synthesis kit (Vazyme Biotech Co)

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Vazyme Biotech Co primescript 1st strand cdna synthesis kit
Primescript 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+ii+reverse+transcriptase+kit/Vazyme+LAmp+Master+Mix/pm37739021-110-6-13
Average 95 stars, based on 65 article reviews

primescript 1st strand cdna synthesis kit - by Bioz Stars, 2026-11

95/100 stars

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RNA Extraction:

Article Title: The optimization of compound enzyme process for enzymatic hydrolyzate from black soldier fly (Hermetia illucens): Antioxidant and immune activity investigation in vitro and in vivo.
Article Snippet: The black soldier fly (BSF) is a promising edible insect, recognized for its high nutritional value and beneficial health effects.. This study was the first time to investigate the optimal conditions for the enzymatic hydrolysis of BSF (EBSF) by compound enzyme method and EBSF's reparative effects on hepatic injuries.. The findings indicated a ratio of alkaline proteinase, trypsin, and papain of 1:1:1, with an enzyme concentration of 6 %, a substrate concentration of 20 %, a temperature of 63 C, and a hydrolysis time of 5 h, yielded the highest amount of EBSF peptides.

Reverse Transcription:

Real-time Polymerase Chain Reaction:

Polymerase Chain Reaction:

Article Title: Molecular mechanism and risk assessment of Rhizoctonia solani resistance to the SDHI fungicide isopyrazam.
Article Snippet: Rice sheath blight is one of the three major rice diseases worldwide, and the succinate dehydrogenase inhibitor (SDHI) fungicide isopyrazam has not yet been registered for its control in China.. This study investigated the sensitivity of Rhizoctonia solani to isopyrazam and assessed its resistance risk.. Specifically, the sensitivity of 113 R. solani isolates to isopyrazam was evaluated, with EC50 values ranging from 0.0018 to 0.0336 μg/mL and an average of 0.0101 ± 0.0064 μg/mL.

Article Title: Detection of Porcine Circovirus Type 3 in Free-Ranging Wild Boars and Ticks in Jiangsu Province, China
Article Snippet: .. Each 50 μL PCR reaction contained 1 μL of sample DNA template, 25 μL of Vazyme Lamp Master Mix (Vazyme, Nanjing, China), and 0.4 μM of each primer. .. The PCR conditions were as follows: pre-denaturation at 94 ◦C for 5 min, followed by 35 cycles of denaturation at 94 ◦C for 30 s, annealing for 45 s, and extension at 72 ◦C for 40 s, with a final extension at 72 ◦C for 7 min. PCR products were purified using the Gel Extraction Kit (Omega Bio-tek, Norcross, USA) and subsequently cloned into the pEASY-Blunt Simple Cloning Vector (TransGen Biotech, Beijing, China).

Article Title: The miR ‐6631‐5p/ ACYP2 Axis Orchestrates Myogenesis and Slow‐Twitch Muscle Fiber Specification in Broilers
Article Snippet: Poultry skeletal muscle fiber composition, primarily defined by oxidative slowtwitch and glycolytic fasttwitch fibers, is a major determinant of meat quality.. Yet the miRNAmRNA regulatory mechanisms controlling fiber type specification remain largely unresolved.. Here, we compared the transcriptomic profiles of pectoralis major (PM) and soleus muscle (SM) in Tianfu broilers and identified ggamiR66315p as a key slowtwitchassociated microRNA (miRNA) through small RNA sequencing and weighted gene coexpression network analysis.

Article Title: Detection of Porcine Circovirus Type 3 in Free-Ranging Wild Boars and Ticks in Jiangsu Province, China
Article Snippet: .. Each 50 μL PCR reaction contained 1 μL of sample DNA template, 25 μL of Vazyme Lamp Master Mix (Vazyme, Nanjing, China), and 0.4 μM of each primer. .. The PCR conditions were as follows: pre-denaturation at 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing for 45 s, and extension at 72 °C for 40 s, with a final extension at 72 °C for 7 min. PCR products were purified using the Gel Extraction Kit (Omega Bio-tek, Norcross, USA) and subsequently cloned into the pEASY-Blunt Simple Cloning Vector (TransGen Biotech, Beijing, China).

RT Lamp Assay:

Article Title: ColorDetect RT-LAMP Assay for the Rapid, Sensitive, and Specific Detection of Porcine Abortion-Associated Pestivirus (PAAPeV)
Article Snippet: .. The ColorDetect RT-LAMP assay was performed using the ColorDetect LAMP/RTLAMP 2× Master Mix (Vazyme Biotech Co., Ltd., Nanjing, China). ..

Article Title: ColorDetect RT-LAMP Assay for the Rapid, Sensitive, and Specific Detection of Porcine Abortion-Associated Pestivirus (PAAPeV)
Article Snippet: .. The ColorDetect RT-LAMP assay was performed using the ColorDetect LAMP/RT-LAMP 2× Master Mix (Vazyme Biotech Co., Ltd., Nanjing, China). ..

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