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ProteoGenix polymorphprep
Polymorphprep, supplied by ProteoGenix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+density+gradient+medium/polymorphprep/pmc10272393-70-26-30
Average 90 stars, based on 1 article reviews
polymorphprep - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Purification:

Article Title: The landscape of immune dysregulation in pediatric sepsis at a single-cell resolution
Article Snippet: .. Leukocytes were purified by combining polymorphonuclear and mononuclear cell layers using Polymorphprep reagent (ProteoGenix, Schiltigheim, France) at room temperature. .. Leukocytes were immediately fixed with 10x fixing reagent (10x Genomics; Pleasanton, CA) and stored in liquid nitrogen until sequencing.

other:

Article Title: Cutibacterium acnes strains associated with bone prosthesis infections cannot evade the host immune system.
Article Snippet: Polymorphonuclear neutrophils were purified from whole human blood using PolymorphprepTM (ProteoGenix).

Article Title: Impairment of regulatory T cell stability in axial spondyloarthritis: role of EZH2 and pSTAT5
Article Snippet: Peripheral blood mononuclear cells (PBMC) were freshly isolated by density gradient centrifugation (Polymorphprep, Proteogenix) from EDTA blood samples from axSpA patients and from healthy donors.

Isolation:

Article Title: Circulating mucosal-like IgA responses increase with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
Article Snippet: After staining, the cells were washed once with PBS-EDTA and fixed with 1% paraformaldehyde before FACS analysis with a 4-laser (405, 488, 561 and 637 nm) Quanteon Novocyte (Agilent Technologies). .. Neutrophils were isolated from EDTA-anticoagulated blood of healthy controls using Polymorphprep (Proteogenix) and 2*10 6 /ml neutrophils in R10 were incubated with 10 μg/ml IgA in 10 μl for 4 hours at 37°C. .. Samples were then incubated with CellROX green dye (Thermo Fisher) and an antibody cocktail ( ) for 30min at RT.

Article Title: Circulating mucosal-like IgA responses associate with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
Article Snippet: After staining, the cells were washed once with PBS-EDTA and fixed with 1% paraformaldehyde before FACS analysis with a 4-laser (405, 488, 561 and 637 nm) Quanteon Novocyte (Agilent Technologies). .. Neutrophils were isolated from EDTA-anticoagulated blood of healthy controls using Polymorphprep (Proteogenix) and 2*10 6 /ml neutrophils in R10 were incubated with 10 μg/ml IgA in 10 μl for 4 hours at 37 °C. .. Samples were then incubated with CellROX green dye (Thermo Fisher) and an antibody cocktail ( ) for 30min at RT.

Article Title: Identification of autoantigens and their potential post-translational modification in EGPA and severe eosinophilic asthma
Article Snippet: .. Venous blood from healthy human volunteers (separate from the study serum donors) was collected in EDTA vacutainer tubes (BD Biosciences) and neutrophils were isolated using the density gradient medium Polymorphprep (Proteogenix) as per the manufacturer’s instructions. .. Isolation of neutrophils was confirmed by flow cytometry using a PerCP/Cyanine5.5 anti-human CD11b antibody (BioLegend) and a PE/Cyanine7 anti-human CD16 antibody (BioLegend).

Article Title: CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan
Article Snippet: Prior to co-culture experiments, NK cells were stimulated overnight with 1000 U/ml IL-2 (Proleukin S, Novartis, Basel, Schweiz). .. PMNs were isolated from 18 mL venous EDTA-anticoagulated blood from healthy donors using PolymorphPrep (ProteoGenix, Schiltigheim, France, #1114683) gradient centrifugation at 590 × g for 30 min. .. The interphase, containing PMNs, was carefully removed and washed with HBSS for 5 min at 590 × g . To lyse remaining erythrocytes, 5 mL erythrocyte lysis buffer (EL buffer, Qiagen, Hilden, Germany, #79217) was added and PMNs were centrifuged, counted with a hemocytometer, and resuspended in RPMI + 10% FCS.

Incubation:

Article Title: Circulating mucosal-like IgA responses increase with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
Article Snippet: After staining, the cells were washed once with PBS-EDTA and fixed with 1% paraformaldehyde before FACS analysis with a 4-laser (405, 488, 561 and 637 nm) Quanteon Novocyte (Agilent Technologies). .. Neutrophils were isolated from EDTA-anticoagulated blood of healthy controls using Polymorphprep (Proteogenix) and 2*10 6 /ml neutrophils in R10 were incubated with 10 μg/ml IgA in 10 μl for 4 hours at 37°C. .. Samples were then incubated with CellROX green dye (Thermo Fisher) and an antibody cocktail ( ) for 30min at RT.

Article Title: Circulating mucosal-like IgA responses associate with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome
Article Snippet: After staining, the cells were washed once with PBS-EDTA and fixed with 1% paraformaldehyde before FACS analysis with a 4-laser (405, 488, 561 and 637 nm) Quanteon Novocyte (Agilent Technologies). .. Neutrophils were isolated from EDTA-anticoagulated blood of healthy controls using Polymorphprep (Proteogenix) and 2*10 6 /ml neutrophils in R10 were incubated with 10 μg/ml IgA in 10 μl for 4 hours at 37 °C. .. Samples were then incubated with CellROX green dye (Thermo Fisher) and an antibody cocktail ( ) for 30min at RT.

Gradient Centrifugation:

Article Title: CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan
Article Snippet: Prior to co-culture experiments, NK cells were stimulated overnight with 1000 U/ml IL-2 (Proleukin S, Novartis, Basel, Schweiz). .. PMNs were isolated from 18 mL venous EDTA-anticoagulated blood from healthy donors using PolymorphPrep (ProteoGenix, Schiltigheim, France, #1114683) gradient centrifugation at 590 × g for 30 min. .. The interphase, containing PMNs, was carefully removed and washed with HBSS for 5 min at 590 × g . To lyse remaining erythrocytes, 5 mL erythrocyte lysis buffer (EL buffer, Qiagen, Hilden, Germany, #79217) was added and PMNs were centrifuged, counted with a hemocytometer, and resuspended in RPMI + 10% FCS.

Article Title: Neutrophils regulate ITPR2 levels in epithelia by direct injection of elastase
Article Snippet: .. Using density gradient centrifugation with PolymorphPrep (Proteogenix, Schiltigheim, France, AN1114683) according to the manufacturer’s instructions, blood was layered in PolymorphPrep solution and centrifuged at 20 °C, 500 x g for 35 minutes without brakes. ..



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