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anti‑il‑18  (Bioss)


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    Bioss anti‑il‑18
    Anti‑Il‑18, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+targeting+il+18/IL-18+Polyclonal+Antibody/pm34676876-69-57-85
    Average 93 stars, based on 7 article reviews
    anti‑il‑18 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole‑kindled mice.
    Article Snippet: Equal amounts of protein (50‐60 μg per lane) were separated by 10% SDS‐PAGE and then transferred to 0.22‐mm PVDF membranes (MilliporeSigma). .. To block non‐specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4 ̊C: Anti‐NLRP3 (cat. no. bs‐10021R), anti‐ASC (cat. no. bs‐6741R), anti‐IL‐1β, (cat. no. bs‐0812R) and anti‐caspase‐1p20 (cat. no. bs‐10442R) (all 1:500); anti‐IL‐6, (cat. no. bs‐6309R), anti‐IL‐18 (cat. no. bs‐4988R), anti‐TNF‐α (cat. no. bs‐10802R), anti‐active caspase‐3 (cat. no. bsm‐33199M), anti‐Bax (cat. no. bs‐0127R), anti‐Bcl‐2 (cat. no. bsm‐52304R) and anti‐β‐actin (cat. no. bs‐0061R) (all 1:1,000) (BIOSS); and anti‐β‐actin (cat. no. 66009‐1‐Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris‐buffered saline (TBS) containing 5‰ Tween‐20, and then incubated with goat anti‐mouse IgG 800RD (cat. no. 926‐32210; 1:5,000) or goat anti‐rabbit IgG 680RD (cat. no. 925‐68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141‐00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole-kindled mice
    Article Snippet: Equal amounts of protein (50-60 μ g per lane) were separated by 10% SDS-PAGE and then transferred to 0.22-mm PVDF membranes (MilliporeSigma). .. To block non-specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4°C: Anti-NLRP3 (cat. no. bs-10021R), anti-ASC (cat. no. bs-6741R), anti-IL-1β, (cat. no. bs-0812R) and anti-caspase-1p20 (cat. no. bs-10442R) (all 1:500); anti-IL-6, (cat. no. bs-6309R), anti-IL-18 (cat. no. bs-4988R), anti-TNF-α (cat. no. bs-10802R), anti-active caspase-3 (cat. no. bsm-33199M), anti-Bax (cat. no. bs-0127R), anti-Bcl-2 (cat. no. bsm-52304R) and anti-β-actin (cat. no. bs-0061R) (all 1:1,000) (BIOSS); and anti-β-actin (cat. no. 66009-1-Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris-buffered saline (TBS) containing 5‰ Tween-20, and then incubated with goat anti-mouse IgG 800RD (cat. no. 926-32210; 1:5,000) or goat anti-rabbit IgG 680RD (cat. no. 925-68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141-00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.2-μm polyvinylidene difluoride (PVDF) membranes (10600021, Amersham, Germany). .. Then, 5% bovine serum albumin (BSA) was applied to block non-specific binding at room temperature for 2 h. Next, the membrane was incubated with the following primary antibodies at 4◦C overnight: NLRP3 (bs-10021R, Bioss, China), ASC (bs-6741R, Bioss, China), caspase-1 p20 (bs-10442R, Bioss, China), IL-1β (bs-0812R, Bioss, China), IL-18 (bs-4988R, Bioss, China), TNF-α (bs10802R, Bioss, China), IL-6 (DF-6087, Affinity, USA), Active Caspase3 (bsm-33199M, Bioss, China), and β-actin (66009-1- Ig, Proteintech, USA) (all 1:1000). .. After washing three times in 0.1% tris-buffered saline containing 5% Tween-20, membranes were incubated in appropriate secondary antibodies at room temperature for 2 h: Goat anti-Mouse (1:4000, 926-32210, LICOR, USA) and Goat anti-Rabbit (1:4000, 926-32211, LI-COR, USA).

    Binding Assay:

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole‑kindled mice.
    Article Snippet: Equal amounts of protein (50‐60 μg per lane) were separated by 10% SDS‐PAGE and then transferred to 0.22‐mm PVDF membranes (MilliporeSigma). .. To block non‐specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4 ̊C: Anti‐NLRP3 (cat. no. bs‐10021R), anti‐ASC (cat. no. bs‐6741R), anti‐IL‐1β, (cat. no. bs‐0812R) and anti‐caspase‐1p20 (cat. no. bs‐10442R) (all 1:500); anti‐IL‐6, (cat. no. bs‐6309R), anti‐IL‐18 (cat. no. bs‐4988R), anti‐TNF‐α (cat. no. bs‐10802R), anti‐active caspase‐3 (cat. no. bsm‐33199M), anti‐Bax (cat. no. bs‐0127R), anti‐Bcl‐2 (cat. no. bsm‐52304R) and anti‐β‐actin (cat. no. bs‐0061R) (all 1:1,000) (BIOSS); and anti‐β‐actin (cat. no. 66009‐1‐Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris‐buffered saline (TBS) containing 5‰ Tween‐20, and then incubated with goat anti‐mouse IgG 800RD (cat. no. 926‐32210; 1:5,000) or goat anti‐rabbit IgG 680RD (cat. no. 925‐68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141‐00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole-kindled mice
    Article Snippet: Equal amounts of protein (50-60 μ g per lane) were separated by 10% SDS-PAGE and then transferred to 0.22-mm PVDF membranes (MilliporeSigma). .. To block non-specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4°C: Anti-NLRP3 (cat. no. bs-10021R), anti-ASC (cat. no. bs-6741R), anti-IL-1β, (cat. no. bs-0812R) and anti-caspase-1p20 (cat. no. bs-10442R) (all 1:500); anti-IL-6, (cat. no. bs-6309R), anti-IL-18 (cat. no. bs-4988R), anti-TNF-α (cat. no. bs-10802R), anti-active caspase-3 (cat. no. bsm-33199M), anti-Bax (cat. no. bs-0127R), anti-Bcl-2 (cat. no. bsm-52304R) and anti-β-actin (cat. no. bs-0061R) (all 1:1,000) (BIOSS); and anti-β-actin (cat. no. 66009-1-Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris-buffered saline (TBS) containing 5‰ Tween-20, and then incubated with goat anti-mouse IgG 800RD (cat. no. 926-32210; 1:5,000) or goat anti-rabbit IgG 680RD (cat. no. 925-68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141-00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.2-μm polyvinylidene difluoride (PVDF) membranes (10600021, Amersham, Germany). .. Then, 5% bovine serum albumin (BSA) was applied to block non-specific binding at room temperature for 2 h. Next, the membrane was incubated with the following primary antibodies at 4◦C overnight: NLRP3 (bs-10021R, Bioss, China), ASC (bs-6741R, Bioss, China), caspase-1 p20 (bs-10442R, Bioss, China), IL-1β (bs-0812R, Bioss, China), IL-18 (bs-4988R, Bioss, China), TNF-α (bs10802R, Bioss, China), IL-6 (DF-6087, Affinity, USA), Active Caspase3 (bsm-33199M, Bioss, China), and β-actin (66009-1- Ig, Proteintech, USA) (all 1:1000). .. After washing three times in 0.1% tris-buffered saline containing 5% Tween-20, membranes were incubated in appropriate secondary antibodies at room temperature for 2 h: Goat anti-Mouse (1:4000, 926-32210, LICOR, USA) and Goat anti-Rabbit (1:4000, 926-32211, LI-COR, USA).

    Membrane:

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole‑kindled mice.
    Article Snippet: Equal amounts of protein (50‐60 μg per lane) were separated by 10% SDS‐PAGE and then transferred to 0.22‐mm PVDF membranes (MilliporeSigma). .. To block non‐specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4 ̊C: Anti‐NLRP3 (cat. no. bs‐10021R), anti‐ASC (cat. no. bs‐6741R), anti‐IL‐1β, (cat. no. bs‐0812R) and anti‐caspase‐1p20 (cat. no. bs‐10442R) (all 1:500); anti‐IL‐6, (cat. no. bs‐6309R), anti‐IL‐18 (cat. no. bs‐4988R), anti‐TNF‐α (cat. no. bs‐10802R), anti‐active caspase‐3 (cat. no. bsm‐33199M), anti‐Bax (cat. no. bs‐0127R), anti‐Bcl‐2 (cat. no. bsm‐52304R) and anti‐β‐actin (cat. no. bs‐0061R) (all 1:1,000) (BIOSS); and anti‐β‐actin (cat. no. 66009‐1‐Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris‐buffered saline (TBS) containing 5‰ Tween‐20, and then incubated with goat anti‐mouse IgG 800RD (cat. no. 926‐32210; 1:5,000) or goat anti‐rabbit IgG 680RD (cat. no. 925‐68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141‐00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole-kindled mice
    Article Snippet: Equal amounts of protein (50-60 μ g per lane) were separated by 10% SDS-PAGE and then transferred to 0.22-mm PVDF membranes (MilliporeSigma). .. To block non-specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4°C: Anti-NLRP3 (cat. no. bs-10021R), anti-ASC (cat. no. bs-6741R), anti-IL-1β, (cat. no. bs-0812R) and anti-caspase-1p20 (cat. no. bs-10442R) (all 1:500); anti-IL-6, (cat. no. bs-6309R), anti-IL-18 (cat. no. bs-4988R), anti-TNF-α (cat. no. bs-10802R), anti-active caspase-3 (cat. no. bsm-33199M), anti-Bax (cat. no. bs-0127R), anti-Bcl-2 (cat. no. bsm-52304R) and anti-β-actin (cat. no. bs-0061R) (all 1:1,000) (BIOSS); and anti-β-actin (cat. no. 66009-1-Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris-buffered saline (TBS) containing 5‰ Tween-20, and then incubated with goat anti-mouse IgG 800RD (cat. no. 926-32210; 1:5,000) or goat anti-rabbit IgG 680RD (cat. no. 925-68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141-00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.2-μm polyvinylidene difluoride (PVDF) membranes (10600021, Amersham, Germany). .. Then, 5% bovine serum albumin (BSA) was applied to block non-specific binding at room temperature for 2 h. Next, the membrane was incubated with the following primary antibodies at 4◦C overnight: NLRP3 (bs-10021R, Bioss, China), ASC (bs-6741R, Bioss, China), caspase-1 p20 (bs-10442R, Bioss, China), IL-1β (bs-0812R, Bioss, China), IL-18 (bs-4988R, Bioss, China), TNF-α (bs10802R, Bioss, China), IL-6 (DF-6087, Affinity, USA), Active Caspase3 (bsm-33199M, Bioss, China), and β-actin (66009-1- Ig, Proteintech, USA) (all 1:1000). .. After washing three times in 0.1% tris-buffered saline containing 5% Tween-20, membranes were incubated in appropriate secondary antibodies at room temperature for 2 h: Goat anti-Mouse (1:4000, 926-32210, LICOR, USA) and Goat anti-Rabbit (1:4000, 926-32211, LI-COR, USA).

    Incubation:

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole‑kindled mice.
    Article Snippet: Equal amounts of protein (50‐60 μg per lane) were separated by 10% SDS‐PAGE and then transferred to 0.22‐mm PVDF membranes (MilliporeSigma). .. To block non‐specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4 ̊C: Anti‐NLRP3 (cat. no. bs‐10021R), anti‐ASC (cat. no. bs‐6741R), anti‐IL‐1β, (cat. no. bs‐0812R) and anti‐caspase‐1p20 (cat. no. bs‐10442R) (all 1:500); anti‐IL‐6, (cat. no. bs‐6309R), anti‐IL‐18 (cat. no. bs‐4988R), anti‐TNF‐α (cat. no. bs‐10802R), anti‐active caspase‐3 (cat. no. bsm‐33199M), anti‐Bax (cat. no. bs‐0127R), anti‐Bcl‐2 (cat. no. bsm‐52304R) and anti‐β‐actin (cat. no. bs‐0061R) (all 1:1,000) (BIOSS); and anti‐β‐actin (cat. no. 66009‐1‐Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris‐buffered saline (TBS) containing 5‰ Tween‐20, and then incubated with goat anti‐mouse IgG 800RD (cat. no. 926‐32210; 1:5,000) or goat anti‐rabbit IgG 680RD (cat. no. 925‐68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141‐00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole-kindled mice
    Article Snippet: Equal amounts of protein (50-60 μ g per lane) were separated by 10% SDS-PAGE and then transferred to 0.22-mm PVDF membranes (MilliporeSigma). .. To block non-specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4°C: Anti-NLRP3 (cat. no. bs-10021R), anti-ASC (cat. no. bs-6741R), anti-IL-1β, (cat. no. bs-0812R) and anti-caspase-1p20 (cat. no. bs-10442R) (all 1:500); anti-IL-6, (cat. no. bs-6309R), anti-IL-18 (cat. no. bs-4988R), anti-TNF-α (cat. no. bs-10802R), anti-active caspase-3 (cat. no. bsm-33199M), anti-Bax (cat. no. bs-0127R), anti-Bcl-2 (cat. no. bsm-52304R) and anti-β-actin (cat. no. bs-0061R) (all 1:1,000) (BIOSS); and anti-β-actin (cat. no. 66009-1-Ig; 1:5,000; ProteinTech Group, Inc.). .. Next, the membranes were washed with Tris-buffered saline (TBS) containing 5‰ Tween-20, and then incubated with goat anti-mouse IgG 800RD (cat. no. 926-32210; 1:5,000) or goat anti-rabbit IgG 680RD (cat. no. 925-68071; 1:5,000) (both BD Biosciences) at RT for 1.5 h. Finally, Odyssey CLX software (9141-00; BD Biosciences) was used for imaging, and protein signals were quantified using ImageJ 8.0 software (National Institutes of Health).

    Article Title: Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
    Article Snippet: Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.2-μm polyvinylidene difluoride (PVDF) membranes (10600021, Amersham, Germany). .. Then, 5% bovine serum albumin (BSA) was applied to block non-specific binding at room temperature for 2 h. Next, the membrane was incubated with the following primary antibodies at 4◦C overnight: NLRP3 (bs-10021R, Bioss, China), ASC (bs-6741R, Bioss, China), caspase-1 p20 (bs-10442R, Bioss, China), IL-1β (bs-0812R, Bioss, China), IL-18 (bs-4988R, Bioss, China), TNF-α (bs10802R, Bioss, China), IL-6 (DF-6087, Affinity, USA), Active Caspase3 (bsm-33199M, Bioss, China), and β-actin (66009-1- Ig, Proteintech, USA) (all 1:1000). .. After washing three times in 0.1% tris-buffered saline containing 5% Tween-20, membranes were incubated in appropriate secondary antibodies at room temperature for 2 h: Goat anti-Mouse (1:4000, 926-32210, LICOR, USA) and Goat anti-Rabbit (1:4000, 926-32211, LI-COR, USA).



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