streptavidin r phycoerythrin conjugate (Agilent technologies)

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Agilent technologies streptavidin r phycoerythrin conjugate

Fig. 2. The workflow of the Quantamatrix Multiplexed Assay Platform (QMAP) system. (a) QMAP kit, different types of shaped-disc graphically encoded carboxyl-functionalized magnetic microparticles on a glass plate. Target species genes were synthesized using a PCR process for amplification and labelled using biotin-dCTP. (b) Denaturated PCR products and a magnetic microdisc are mixed on a microwell plate, hybridized, washed and R-phycoerythrin-conjugated <t>streptavidin-processed</t> to produce fluorescent signals for auto- matic analysis (40 min/96 samples). (c, d) QMAP image analyser. The fluorescence intensity of all microdiscs in the image was auto- matically measured using the provided QMAP software (version 2.0) and positive fluorescence intensity signal (>500) for the identification of Mycobacterium species was auto-interpreted from Microsoft Excel program (30 s per sample).

Streptavidin R Phycoerythrin Conjugate, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pjrs20/Streptavidin-R-Phycoerythrin/pm28604333-93-30-33
Average 95 stars, based on 239 article reviews

streptavidin r phycoerythrin conjugate - by Bioz Stars, 2026-10

95/100 stars

Images

1) Product Images from "Performance of the Quantamatrix Multiplexed Assay Platform system for the differentiation and identification of Mycobacterium species."

Article Title: Performance of the Quantamatrix Multiplexed Assay Platform system for the differentiation and identification of Mycobacterium species.

Journal: Journal of medical microbiology

doi: 10.1099/jmm.0.000495

Figure Legend Snippet: Fig. 2. The workflow of the Quantamatrix Multiplexed Assay Platform (QMAP) system. (a) QMAP kit, different types of shaped-disc graphically encoded carboxyl-functionalized magnetic microparticles on a glass plate. Target species genes were synthesized using a PCR process for amplification and labelled using biotin-dCTP. (b) Denaturated PCR products and a magnetic microdisc are mixed on a microwell plate, hybridized, washed and R-phycoerythrin-conjugated streptavidin-processed to produce fluorescent signals for auto- matic analysis (40 min/96 samples). (c, d) QMAP image analyser. The fluorescence intensity of all microdiscs in the image was auto- matically measured using the provided QMAP software (version 2.0) and positive fluorescence intensity signal (>500) for the identification of Mycobacterium species was auto-interpreted from Microsoft Excel program (30 s per sample).

Techniques Used: Synthesized, Amplification, Fluorescence, Software

Incubation:

Article Title: Human immune response to primary cryptosporidiosis parallels murine infection models
Article Snippet: In brief, recombinant Cp17 and Cp23 proteins (Genscript, cat. no. For RQ-0000128619) were biotinylated with an EZ-Link Sulfo–NHS–LC–Biotinylation kit (Thermo Scientific, cat. no. 21435). .. Unreacted Sulfo-NHS-biotin was removed via size exclusion column before incubation with fluorophore-labeled streptavidin (SA-PE for Cp23, SA-APC for Cp17) (Agilent, cat. no. PJRS25; Cedarlane, cat. no. PJ27S). ..

Article Title: Human immune response to primary cryptosporidiosis parallels murine infection models.
Article Snippet: In brief, recombinant Cp17 and Cp23 proteins (Genscript, cat. no. For RQ-0000128619) were biotinylated with an EZ-Link Sulfo–NHS–LC–Biotinyla tion kit (Thermo Scientific, cat. no. 21435). .. Unreacted Sulfo-NHS-biotin was removed via size exclusion column before incubation with fluorophore-labeled streptavidin (SA-PE for Cp23, SA-APC for Cp17) (Agilent, cat. no. PJRS25; Cedarlane, cat. no. PJ27S). ..

Article Title: Transgenic mouse-derived human monoclonal antibodies targeting EBV gp350 and gp42 provide basis for therapeutic development.
Article Snippet: Wells containing hybridomas that displayed at least 50% neutralizing activity were sub-cloned using the ClonePix (Molecular Devices) and re-screened for binding and neutralization. .. Fluorescently labeled streptavidin tetramer production Biotinylated gp42 or gp350 were incubated with either streptavidin-R-phycoerythrin (SA-PE, Agilent) or streptavidin allophycocyanin (SA-APC, Agilent) at a 4:1 molar ratio. .. To produce recombinant mAbs, RNA was extracted from 1 × 10 6 hybridoma cells using the Monarch Total RNA Miniprep Kit, and cDNA encoding the heavy and light chain variable regions of the murine hybridomas were by reverse transcribed and amplified using the procedures outlined in Meyer et al. 102 Amplicons were Sanger sequenced directly or TOPO cloned (Thermo FisherScientific) and then sequenced.

Recombinant:

Article Title: Conjugated pneumococcal vaccination yields broadly functional, mucosal-directed responses detectable up to at least 42 months in older adults.
Article Snippet: .. The most frequent comorbidities in the pre-vaccination and at REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-guinea pig complement C3 goat IgG - FITC MP Biomedicals Cat # 0855385; RRID: AB_2334913 Mouse Anti-Human IgG Fc-PE (JDC-10) SouthernBiotech 9040–09; RRID: AB_2796601 Mouse Anti-Human IgG1 Hinge-PE (4E3) SouthernBiotech 9052–09; RRID: AB_2796621 Mouse Anti-Human IgG2 Fc-PE (31-7-4) SouthernBiotech 9060–09; RRID: AB_2796635 Mouse Anti-Human IgG3 Hinge-PE (HP6050) SouthernBiotech 9210–09; RRID: AB_279670 Mouse Anti-Human IgG4 Fc-PE (HP6025) SouthernBiotech 9200–09; RRID: AB_2796693 Mouse Anti-Human IgA1-PE (B3506B4) SouthernBiotech 9130–09; RRID: AB_2796656 Mouse Anti-Human IgA2-PE (A9604D2) SouthernBiotech 9140–09; RRID: AB_2796664 Mouse Anti-Human IgM-PE (SA-DA4) SouthernBiotech 9020–09; RRID: AB_2796577 Chemicals, Peptides, and Recombinant Proteins Poly-L-Lysine conjugated Pneumococcal serotype 1 Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 3 Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 6A Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 6B Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 7F Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 19A Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 19F Pfizer Custom Synthesized Poly-L-Lysine conjugated Pneumococcal serotype 23F Pfizer Custom Synthesized Human soluble Fcγ2A/293F Duke University Custom Order Human soluble Fcγ2B/293F Duke University Custom Order Human soluble Fcy3A/293F Duke University Custom Order Human soluble Fcy3B/293F Duke University Custom Order Human soluble FcαR/293F Duke University Custom Order Brefeldin A Sigma Aldrich Cat #B7651 LowTox Guinea Pig Complement CedarLane Labs Cat # CL4051 Streptavidin-R-Phycoerythrin Prozyme Cat # PJ31S Deposited Data HSPH GitHub Page – https://github.com/HSPHSystemsSerology/QW20250409 Software and Algorithms iQue Forecyt 9.1 Sartorius 60028 R Studio V 6.0 R Project SCR_000432 Other 384-well HydroSpeed Plate Washer Tecan 30190112 iQue Screener Plus Intellicyt/Sartorius 11811 MagPlex Microspheres Luminex MFG MC12001 12 Cell Reports 45, 117307, May 26, 2026 each vaccinated follow up timepoint in the treatment arms can be found in Table S1. ..

Synthesized:

Polyacrylamide Gel Electrophoresis:

Software:

Labeling:

Article Title: Isolation and characterization of broadly-neutralizing anti-HCMV-gB antibodies from human donors using a prefusion-stabilized HCMV gB variant.
Article Snippet: Excess biotin was removed using Zeba spin desalting columns (Thermo Fisher, Cat# 87766) and used immediately or stored at -80 °C after flash-freezing. .. PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013950 February 5, 2026 22 / 36 Fluorescently labeled streptavidin tetramer production Biotinylated pEW62 was conjugated to either streptavidin-R-phycoerythrin (SA-PE, Agilent, Cat# PJRS301-1) or streptavidin allophycocyanin (SA-APC, Agilent, Cat# PJ27S-1) at 4:1 molar ratio. .. A PE-decoy reagent was produced by conjugating SA-PE to DyLight 650 NHS Ester-(DL650;Thermo Fisher, Cat# 62266) by mixing 1 mg Agilent SA-PE into one vial of 50 μg DL650 and incubating at RT for 60 minutes.

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