nf κb p65 (Signalway Antibody)
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Nf κb P65, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/315+ac+anti+k314+nf%CE%BAb+p65/pm42298689-203-69-66
Average 86 stars, based on 1 article reviews
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other:Article Title: Anti-inflammatory activity and mechanism of action of Banxia Xiexin Decoction on DSS-induced ulcerative colitis in mice. Article Snippet: Ethnopharmacological relevance: Banxia Xiexin Decoction (BXD) is a Traditional Chinese Medicine (TCM) formulation, historically utilized in the treatment of ulcerative colitis (UC).. However, its precise active constituents and the underlying mechanisms of action remain insufficiently understood.. Aim of the study: We herein aimed to explore the molecular mechanisms by which BXD mitigates dextran sodium sulfate (DSS)-induced UC in mice. Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway Article Snippet: Reagents Western Blot:Article Title: Strongylocentrotus nudus egg polysaccharide (SEP) suppresses HBV replication via activation of TLR4-induced immune pathway. Article Snippet: .. Cell lysates were then collected and were used for immunoblotting with anti-TLR4 (College Park, MD, Signalway Antibody), Article Title: Regorefenib induces extrinsic/intrinsic apoptosis and inhibits MAPK/NF‐κB‐modulated tumor progression in bladder cancer in vitro and in vivo Article Snippet: Hygromycin B was purchased from Santa Cruz Biotechnology. .. The primary antibodies were purchased from different companies as described as follows: MMP‐9 (EMD Millipore, Billerica, MA, USA), VEGF (EMD Millipore), C‐FLIP (Cell signaling Technology, Danvers, MA, USA), Phospho‐p44/42 MAPK (Erk1/2) (Cell signaling), Phospho‐p38 MAPK (Thr180/Tyr182) (Cell signaling), p38 MAPK (Cell signaling), tERK (Santa Cruz, CA, USA), Blocking Assay:Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway. Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: Incubation:Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway. Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), Article Title: IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migration Article Snippet: Denatured proteins were separated on 12% denaturing polyacrylamide gels and transferred to nitrocellulose membrane (Hybond C; Amersham, Arlington Heights, IL). .. Membranes were blocked with a 5% (w/v) nonfat dried milk solution containing 10 mM Tris-Cl, pH 7.5, 140 mM NaCl, 1.5 mM MgCl 2 , and 0.1% Tween 20 (TBSTM), and incubated with JAK1 (sc-376996; dilution 1:200), STAT1 (Cell Signaling, #9172; dilution 1:1000), Ser-727 pSTAT1 (Cell Signaling, #8826; dilution 1:1000), p65 NFκB (sc-8008; dilution 1:200), Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: Control:Article Title: IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migration Article Snippet: Denatured proteins were separated on 12% denaturing polyacrylamide gels and transferred to nitrocellulose membrane (Hybond C; Amersham, Arlington Heights, IL). .. Membranes were blocked with a 5% (w/v) nonfat dried milk solution containing 10 mM Tris-Cl, pH 7.5, 140 mM NaCl, 1.5 mM MgCl 2 , and 0.1% Tween 20 (TBSTM), and incubated with JAK1 (sc-376996; dilution 1:200), STAT1 (Cell Signaling, #9172; dilution 1:1000), Ser-727 pSTAT1 (Cell Signaling, #8826; dilution 1:1000), p65 NFκB (sc-8008; dilution 1:200), |
