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Signalway Antibody nf κb p65
Nf κb P65, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/315+ac+anti+k314+nf%CE%BAb+p65/pm42298689-203-69-66
Average 86 stars, based on 1 article reviews
nf κb p65 - by Bioz Stars, 2026-09
86/100 stars

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other:

Article Title: Anti-inflammatory activity and mechanism of action of Banxia Xiexin Decoction on DSS-induced ulcerative colitis in mice.
Article Snippet: Ethnopharmacological relevance: Banxia Xiexin Decoction (BXD) is a Traditional Chinese Medicine (TCM) formulation, historically utilized in the treatment of ulcerative colitis (UC).. However, its precise active constituents and the underlying mechanisms of action remain insufficiently understood.. Aim of the study: We herein aimed to explore the molecular mechanisms by which BXD mitigates dextran sodium sulfate (DSS)-induced UC in mice.

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway
Article Snippet: Reagents NF-κB-p65 Antibody (Cat No. 21014), NF-κB p65 Mouse Monoclonal Antibody (Cat No. 38054), PIM2 Rabbit mAb (Cat No. 56914), IL10 Antibody (Cat No. 38392), and goat anti-rabbit IgG Secondary Antibody (Cat No. L3012) were purchased from Signalway Antibodies (Jiangsu, China).

Western Blot:

Article Title: Strongylocentrotus nudus egg polysaccharide (SEP) suppresses HBV replication via activation of TLR4-induced immune pathway.
Article Snippet: .. Cell lysates were then collected and were used for immunoblotting with anti-TLR4 (College Park, MD, Signalway Antibody), anti-p-p65 (College Park, MD, Signalway Antibody), antip65 (College Park, MD, Signalway Antibody), anti-IRF3 (College Park, MD, Signalway Antibody), anti-p-IRF3 (College Park, MD, Signalway Antibody), anti-HBcAg, and anti-β-actin (Cell Signaling Technology) antibodies for protein analysis. ..

Article Title: Regorefenib induces extrinsic/intrinsic apoptosis and inhibits MAPK/NF‐κB‐modulated tumor progression in bladder cancer in vitro and in vivo
Article Snippet: Hygromycin B was purchased from Santa Cruz Biotechnology. .. The primary antibodies were purchased from different companies as described as follows: MMP‐9 (EMD Millipore, Billerica, MA, USA), VEGF (EMD Millipore), C‐FLIP (Cell signaling Technology, Danvers, MA, USA), Phospho‐p44/42 MAPK (Erk1/2) (Cell signaling), Phospho‐p38 MAPK (Thr180/Tyr182) (Cell signaling), p38 MAPK (Cell signaling), tERK (Santa Cruz, CA, USA), Phospho‐p65 NF‐κB (Ser276) (Signalway Antibody LLC, MD, USA), p65 NF‐κB antibody (Abcam plc, Canary Wharf, London, UK), XIAP (Thermo Fisher Scientific, Fremont, CA, USA), Cyclin D1 (Thermo Fisher Scientific), β‐actin (Santa Cruz), TBP (Abcam plc.) for Western blotting evaluation. ..

Blocking Assay:

Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway.
Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), NF-κB p65 (mouse; 1:1000), p-NF-κB p65 (rabbit; 1:1000; #ab86299, Abcam, USA), NLRP3 (rabbit, 1:1000, #A21906; ABclonal, Wuhan, China), apoptosis- associated speck-like protein containing a CARD (ASC) (rabbit, 1:1000, #A16672; ABclonal, Wuhan, China), Cysteine-aspartic acid protease 1 (Caspase-1) (rabbit, 1:1000, #A0964; ABclonal, Wuhan, China), Cleaved Caspase-1 (rabbit, 1:1000, #89332; CST, USA); IL-1β (rabbit, 1:1000, #31202; CST, USA), β-actin (rabbit, 1:3000, 81115-1-RR; Proteintech, Wuhan, China). .. The membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse/rabbit IgG, 1:4000, SA00001-1, SA00001-2; Proteintech, Wuhan, China).

Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation
Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: p-NF-κB p65 (Ser536, 1:1000; Signalway), NLRP3 (SC06-23, 1:1500; HUABIO), ASC (HY- P80548 ; MCE), NF-κB p65 (L8F6, 1:500; Cell Signaling), caspase-1 (SU40-07, 1:80,000; HUABIO), cleaved-caspase-1 (Asp296, 1:1000; Affinity), and GAPDH (60004-1-Ig, 1:50000; Proteintech). .. On the following day, membranes were washed and exposed to secondary antibodies (SA00001-1; SA00001-2, 1:5000; Proteintech) for 1 hour at room temperature, and signals were subsequently detected using ECL chemiluminescent solution (BMU102; Abbkine).

Incubation:

Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway.
Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), NF-κB p65 (mouse; 1:1000), p-NF-κB p65 (rabbit; 1:1000; #ab86299, Abcam, USA), NLRP3 (rabbit, 1:1000, #A21906; ABclonal, Wuhan, China), apoptosis- associated speck-like protein containing a CARD (ASC) (rabbit, 1:1000, #A16672; ABclonal, Wuhan, China), Cysteine-aspartic acid protease 1 (Caspase-1) (rabbit, 1:1000, #A0964; ABclonal, Wuhan, China), Cleaved Caspase-1 (rabbit, 1:1000, #89332; CST, USA); IL-1β (rabbit, 1:1000, #31202; CST, USA), β-actin (rabbit, 1:3000, 81115-1-RR; Proteintech, Wuhan, China). .. The membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse/rabbit IgG, 1:4000, SA00001-1, SA00001-2; Proteintech, Wuhan, China).

Article Title: IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migration
Article Snippet: Denatured proteins were separated on 12% denaturing polyacrylamide gels and transferred to nitrocellulose membrane (Hybond C; Amersham, Arlington Heights, IL). .. Membranes were blocked with a 5% (w/v) nonfat dried milk solution containing 10 mM Tris-Cl, pH 7.5, 140 mM NaCl, 1.5 mM MgCl 2 , and 0.1% Tween 20 (TBSTM), and incubated with JAK1 (sc-376996; dilution 1:200), STAT1 (Cell Signaling, #9172; dilution 1:1000), Ser-727 pSTAT1 (Cell Signaling, #8826; dilution 1:1000), p65 NFκB (sc-8008; dilution 1:200), K314/315 ac-p65 NFκB (Signalway Antibody, HW136; dilution 1:200), and control actin (Sigma, A5060; dilution 1:2000) antibodies diluted in TBSTM. .. After washing, the membranes were incubated with HRP-labeled secondary anti-rabbit (Novus NB7185) or anti-mouse (Novus NB7570) antibodies (dilution 1:5000) and the labeled proteins were detected using the ECL detection system (Amersham, Arlington Heights, IL).

Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation
Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: p-NF-κB p65 (Ser536, 1:1000; Signalway), NLRP3 (SC06-23, 1:1500; HUABIO), ASC (HY- P80548 ; MCE), NF-κB p65 (L8F6, 1:500; Cell Signaling), caspase-1 (SU40-07, 1:80,000; HUABIO), cleaved-caspase-1 (Asp296, 1:1000; Affinity), and GAPDH (60004-1-Ig, 1:50000; Proteintech). .. On the following day, membranes were washed and exposed to secondary antibodies (SA00001-1; SA00001-2, 1:5000; Proteintech) for 1 hour at room temperature, and signals were subsequently detected using ECL chemiluminescent solution (BMU102; Abbkine).

Control:

Article Title: IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migration
Article Snippet: Denatured proteins were separated on 12% denaturing polyacrylamide gels and transferred to nitrocellulose membrane (Hybond C; Amersham, Arlington Heights, IL). .. Membranes were blocked with a 5% (w/v) nonfat dried milk solution containing 10 mM Tris-Cl, pH 7.5, 140 mM NaCl, 1.5 mM MgCl 2 , and 0.1% Tween 20 (TBSTM), and incubated with JAK1 (sc-376996; dilution 1:200), STAT1 (Cell Signaling, #9172; dilution 1:1000), Ser-727 pSTAT1 (Cell Signaling, #8826; dilution 1:1000), p65 NFκB (sc-8008; dilution 1:200), K314/315 ac-p65 NFκB (Signalway Antibody, HW136; dilution 1:200), and control actin (Sigma, A5060; dilution 1:2000) antibodies diluted in TBSTM. .. After washing, the membranes were incubated with HRP-labeled secondary anti-rabbit (Novus NB7185) or anti-mouse (Novus NB7570) antibodies (dilution 1:5000) and the labeled proteins were detected using the ECL detection system (Amersham, Arlington Heights, IL).



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Image Search Results


The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Expressing

The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Activity Assay