tsa dig (Akoya Biosciences)
Akoya Biosciences is a verified supplier 
Akoya Biosciences manufactures this product 
94
Structured Review
Akoya Biosciences
tsa dig
Tsa Dig, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nel748001kt/TSA+Plus+DIG/pmc11442383-92-7-14
Average 94 stars, based on 27 article reviews
Tsa Dig, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nel748001kt/TSA+Plus+DIG/pmc11442383-92-7-14
Average 94 stars, based on 27 article reviews
tsa dig - by Bioz Stars,
2026-10
94/100 stars
Images
Related Articles
Fluorescence:Article Title: The spatial transcriptome of the late-stage embryonic and postnatal mouse brain reveals spatiotemporal molecular markers Article Snippet: Next, the sections were treated with the protease for 10 min at 40 °C and exposed to the Nr4a2 , Etl4 , and Kcnip1 probes (Advanced Cell Diagnostics) for 2 h at 40 °C before signal amplification. .. The fluorescence signal was further amplified using the Article Title: The spatial transcriptome of the late-stage embryonic and postnatal mouse brain reveals spatiotemporal molecular markers. Article Snippet: Next, the sections were treated with the protease for 10 min at 40 °C and exposed to the Nr4a2, Etl4, and Kcnip1 probes (Advanced Cell Diagnostics) for 2 h at 40 °C before signal amplification. .. The fluorescence signal was further amplified using the Amplification:Article Title: The spatial transcriptome of the late-stage embryonic and postnatal mouse brain reveals spatiotemporal molecular markers Article Snippet: Next, the sections were treated with the protease for 10 min at 40 °C and exposed to the Nr4a2 , Etl4 , and Kcnip1 probes (Advanced Cell Diagnostics) for 2 h at 40 °C before signal amplification. .. The fluorescence signal was further amplified using the Article Title: Comparative analysis of the tumor immune microenvironment between thymoma and thymic carcinoma. Article Snippet: Background: Thymic epithelial tumors (TETs) often linked with autoimmune syndromes such as myasthenia gravis (MG), have an underexplored tumor immune microenvironment (TIME), limiting the effective use of Immune Checkpoint Inhibitors.. This study aims to address the immunology of TETs, focusing on the prognostic significance of TIME and its association with MG.. This study investigates the TIME in TET subtypes, thymic carcinoma (TC) and thymoma, assesses their prognoses, and examines the relationship between thymoma’s immune microenvironment and MG. Methods: This study included 157 patients with TET who underwent surgical resection at Juntendo Hospital between January 2012 and September 2020. Article Title: Evolutionary trajectories of teleost olfactory signaling genes shaped by long-term redundancy after whole-genome duplication Article Snippet: .. For dual-color detection, the procedures were generally identical to those for single-color detection, except that signal amplification was performed using the Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Article Title: The spatial transcriptome of the late-stage embryonic and postnatal mouse brain reveals spatiotemporal molecular markers. Article Snippet: Next, the sections were treated with the protease for 10 min at 40 °C and exposed to the Nr4a2, Etl4, and Kcnip1 probes (Advanced Cell Diagnostics) for 2 h at 40 °C before signal amplification. .. The fluorescence signal was further amplified using the Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Incubation:Article Title: PASTA: Versatile Tyramine-oligonucleotide Amplification for Multi-modal Spatial Biology Article Snippet: Meanwhile, tyramine oligo or fluorophore (for 4-channel positive control, see Table S3 ) were diluted in TSA diluent buffer (FP1498, Akoya Bio-sciences) to a concentration of 5 μM (tyramine oligo) or 1:100 (tyramine fluorophores) and added to the tissue for incubation at room temperature for 20 minutes in the dark. .. Slides were rinsed and washed twice, after which 3 drops of HRP blocker were added to each tissue and incubated at 40°C for 15 minutes followed by a final rinse and wash. For the fluorophore controls, the following tyramine conjugates were deposited in order (see Table S3 ): Article Title: Comparative analysis of the tumor immune microenvironment between thymoma and thymic carcinoma. Article Snippet: Background: Thymic epithelial tumors (TETs) often linked with autoimmune syndromes such as myasthenia gravis (MG), have an underexplored tumor immune microenvironment (TIME), limiting the effective use of Immune Checkpoint Inhibitors.. This study aims to address the immunology of TETs, focusing on the prognostic significance of TIME and its association with MG.. This study investigates the TIME in TET subtypes, thymic carcinoma (TC) and thymoma, assesses their prognoses, and examines the relationship between thymoma’s immune microenvironment and MG. Methods: This study included 157 patients with TET who underwent surgical resection at Juntendo Hospital between January 2012 and September 2020. Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Staining:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Labeling:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Blocking Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Activation Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Binding Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. |