cd45 ptprc (Novus Biologicals)
Novus Biologicals is a verified supplier 
Novus Biologicals manufactures this product 
94
Structured Review
Novus Biologicals
cd45 ptprc
Cd45 Ptprc, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-77417/CD45+Antibody+(30-F11)+-+BSA+Free/10__1523_slash_jneurosci__0555___21__2021-110-62-70
Average 94 stars, based on 70 article reviews
Cd45 Ptprc, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-77417/CD45+Antibody+(30-F11)+-+BSA+Free/10__1523_slash_jneurosci__0555___21__2021-110-62-70
Average 94 stars, based on 70 article reviews
cd45 ptprc - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Retinal Ganglion Cell Axon Regeneration Requires Complement and Myeloid Cell Activity within the Optic Nerve"
Article Title: Retinal Ganglion Cell Axon Regeneration Requires Complement and Myeloid Cell Activity within the Optic Nerve
Journal: The Journal of Neuroscience
doi: 10.1523/jneurosci.0555-21.2021
Figure Legend Snippet: Figure 5. Marked increase in CR3 near the site of ONI. Complement C3b phagocytosis receptor CR3 is microglia/monocyte-specific, and CR31 microglia/monocyte number and phagocytic activation increase within the injured optic nerve. A, Representative images of CR3 immunolabeled uninjured (–) and injured (1, 3, 5, 7, 14 DPI) optic nerves at the injury site. Indicated regions (☐) are shown enlarged to the right of each image. Note the accumulation of CR31 cells and the increased cellular signal intensity following ONI. B, C, Quantitation confirms that the approximate number of CR31 cells (B) increases rapidly (by 1 DPI) and is sustained for at least 14 DPI, with a 30-fold peak at 5 DPI; likewise, CR3 immunofluorescence intensity (C) is elevated at all postinjury time points evaluated (1-14 DPI). D, The specificity of the CR3 (CD11b) antibody was verified using 14 DPI nerves from CR31/1 (left) and CR3/ mice (right). E, The approximate number of Iba11 microglia/monocytes in the nerve increases dramatically by 5 DPI and remains elevated until at least 14 DPI. F, Quantitation of CR31Iba1 (red), CR3-Iba11 (green), and colocalized CR31Iba11 (yellow) cells at/near the injury site. While most CR31 optic nerve cells are microglia, some CR31 cells in the injured nerve are likely infiltrating peripheral blood monocytes. G, CR3 (red) colocalization with Iba1 (green; microglia) at nerve injury site (3, 5 DPI), in the distal nerve (3 DPI), and in the chiasm (14 DPI). H–J, Presence of myeloid cell markers CD206 (H), CD45 (I), and P2RY12 (J) in 14 DPI optic nerves. Iba11 and CR31 microglia/monocytes at/near the injury are large, round, and lack processes: morphologic changes associated with phagocytic activation. L, Similarly, these Iba11 (green) microglia/monocytes express strikingly high levels of lysosome marker CD68 (red; observed 3-14 DPI, 14 DPI shown). M, C1q deletion dampens the CR31 cell response to injury plus zymosan 1 CPT-cAMP treatment at 5 and 14 DPI. N, Pro-regenerative treatments may augment the CR31 cell response in the injured nerve at 14 DPI. Scale bars: A, 50 mm, 10 mm; D, G, H, I, J, L, 50 mm. Graphs represent either mean 6 SEM or 25th to 75th percentile (box), median (line), range (whiskers), and individual data points (circles). B, C, E, K, *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001, one-way ANOVA with Dunnett post-test vs uninjured or untreated. J, **p , 0.01, t test.
Techniques Used: Activation Assay, Immunolabeling, Quantitation Assay, Immunofluorescence, Marker
Related Articles
Immunohistochemistry:Article Title: A pontine-specific niche supports de novo gliomagenesis. Article Snippet: noclonal anti-phospho-AKT (Ser473) Cell Signaling Technology Cat#4060 S; RRID:AB_2315049 Rabbit monoclonal anti-H3K27me3 Cell Signaling Technology Cat#9733 S; RRID: AB_2616029 Rat monoclonal anti-CD45 Staining:Article Title: A pontine-specific niche supports de novo gliomagenesis. Article Snippet: noclonal anti-phospho-AKT (Ser473) Cell Signaling Technology Cat#4060 S; RRID:AB_2315049 Rabbit monoclonal anti-H3K27me3 Cell Signaling Technology Cat#9733 S; RRID: AB_2616029 Rat monoclonal anti-CD45 Incubation:Article Title: A pontine-specific niche supports de novo gliomagenesis. Article Snippet: noclonal anti-phospho-AKT (Ser473) Cell Signaling Technology Cat#4060 S; RRID:AB_2315049 Rabbit monoclonal anti-H3K27me3 Cell Signaling Technology Cat#9733 S; RRID: AB_2616029 Rat monoclonal anti-CD45 Immunostaining:Article Title: A pontine-specific niche supports de novo gliomagenesis. Article Snippet: noclonal anti-phospho-AKT (Ser473) Cell Signaling Technology Cat#4060 S; RRID:AB_2315049 Rabbit monoclonal anti-H3K27me3 Cell Signaling Technology Cat#9733 S; RRID: AB_2616029 Rat monoclonal anti-CD45 |