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nbqx  (Alomone Labs)


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    Structured Review

    Alomone Labs nbqx
    Nbqx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n-185/NBQX/pmc05611379-57-28-29
    Average 94 stars, based on 28 article reviews
    nbqx - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: GABA B Receptors Mediate Intracellular Calcium Release in Astrocytes of the Prefrontal Cortex
    Article Snippet: .. The reagents D‐2‐amino‐5‐phosphonovaleric acid (D‐APV; antagonist of NMDA receptors; #D‐145; working concentration: 100 μM), 2,3‐dioxo‐6‐nitro‐1,2,3,4‐tetrahydrobenzo[f]quinoxaline‐7‐sulfonamide (NBQX; antagonist of AMPA/kainate receptors; #N‐186; working concentration: 10 μM), tetrodotoxin (TTX; inhibiting voltage‐gated sodium channels; #T‐550; working concentration: 0.5 μM), and gabazine (antagonist of GABA A and Glycine receptors, #G‐215; working concentration: 5 μM) were purchased from Alomone Labs (Jerusalem, Israel). .. The compounds ( R , S )‐4‐amino‐3‐(4‐chlorophenyl)butanoic acid (R,S)‐baclofen; GABA B receptor agonist; #ab120149; working concentration: 200 μM); and cyclopiazonic acid (CPA; Ca 2+ ‐ATPase inhibitor; #ab120300; working concentration: 20 μM) were obtained from Abcam (Cambridge, UK).

    Article Title: Repeated administration of AMPA/kainate antagonists transiently suppresses nicotine-seeking in an operant rat model.
    Article Snippet: .. NBQX was purchased from Alomone Labs (Jerusalem, Israel) and dissolved in saline to obtain 5 mg/ml concentration. ..

    Transferring:

    Article Title: Oxytocin Improves Autistic Behaviors by Positively Shifting GABA Reversal Potential via NKCC1 in Early-Postnatal-Stage.
    Article Snippet: .. The sGABA-PSCs and mGABA-PSCs were recorded at a holding potential of −70 mV using high Cl− pipette solution in the presence of DAPV (50 μm; Tocris Bioscience, Bristol, UK, cat. number: No. 0106/1) and NBQX (20 μm; Tocris Bioscience, Bristol, UK, cat. number: No. 0373/10), with or without TTX (0.3 μm; Alomone Labs, Israel, cat. number: 18660-81- 6), respectively. .. Moreover, the sGlut-PSCs and mGlut-PSCs were recorded at a holding potential of −70 mV using a Cs+-methanesulfonate-based internal solution in the presence of SR95531 (20 μm; Tocris Bioscience, cat. number: No. 1262/10), with or without TTX (0.3 μm), respectively.

    Article Title: Oxytocin Improves Autistic Behaviors by Positively Shifting GABA Reversal Potential via NKCC1 in Early‐Postnatal‐Stage
    Article Snippet: .. The sGABA‐PSCs and mGABA‐PSCs were recorded at a holding potential of −70 mV using high Cl − pipette solution in the presence of D‐APV (50 μ m ; Tocris Bioscience, Bristol, UK, cat. number: No. 0106/1) and NBQX (20 μ m ; Tocris Bioscience, Bristol, UK, cat. number: No. 0373/10), with or without TTX (0.3 μ m ; Alomone Labs, Israel, cat. number: 18660‐81‐6), respectively. .. Moreover, the sGlut‐PSCs and mGlut‐PSCs were recorded at a holding potential of −70 mV using a Cs + ‐methanesulfonate‐based internal solution in the presence of SR95531 (20 μ m ; Tocris Bioscience, cat. number: No. 1262/10), with or without TTX (0.3 μ m ), respectively.

    Inhibition:

    Article Title: Synaptic Drive onto Inhibitory and Excitatory Principal Neurons of the Mouse Lateral Superior Olive
    Article Snippet: Cells were held at −70 mV during synaptic data collection. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated glutamatergic inputs were isolated by bath-applied pharmacological blockade of glycine receptors (1 μM strychnine, Sigma), GABA A receptors (2 μM gabazine, Sigma), GABA B receptors (1 μM CGP55845 , Sigma), and NMDA receptors (25 μM d -AP5, Alomone Labs). .. Glycine receptor (GlyR)-mediated inhibition was isolated by bath-applied pharmacological blockade of GABA A receptors (2 μM gabazine, Sigma), GABA B receptors (1 μM CGP55845 , Sigma), NMDA receptors (25 μM d -AP5, Alomone Labs), and AMPA/kainite receptors (10 μM NBQX, Alomone Labs). ..

    Article Title: Synaptic drive onto inhibitory and excitatory principal neurons of the mouse lateral superior olive
    Article Snippet: Cells were held at −70 mV during synaptic data collection. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated glutamatergic inputs were isolated by bath-applied pharmacological blockade of glycine receptors (1 μM strychnine, Sigma), GABAA receptors (2 μM gabazine, Sigma), GABAB receptors (1 μM CGP55845, Sigma), and NMDA receptors (25 μM D-AP5, Alomone Labs). .. Glycine receptor (GlyR)-mediated inhibition was isolated by bath-applied pharmacological blockade of GABAA receptors (2 μM gabazine, Sigma), GABAB receptors (1 μMCGP55845, Sigma), NMDA receptors (25 μM D-AP5, Alomone Labs), and AMPA/kainite receptors (10 μM NBQX, Alomone Labs). ..

    Isolation:

    Article Title: Synaptic Drive onto Inhibitory and Excitatory Principal Neurons of the Mouse Lateral Superior Olive
    Article Snippet: Cells were held at −70 mV during synaptic data collection. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated glutamatergic inputs were isolated by bath-applied pharmacological blockade of glycine receptors (1 μM strychnine, Sigma), GABA A receptors (2 μM gabazine, Sigma), GABA B receptors (1 μM CGP55845 , Sigma), and NMDA receptors (25 μM d -AP5, Alomone Labs). .. Glycine receptor (GlyR)-mediated inhibition was isolated by bath-applied pharmacological blockade of GABA A receptors (2 μM gabazine, Sigma), GABA B receptors (1 μM CGP55845 , Sigma), NMDA receptors (25 μM d -AP5, Alomone Labs), and AMPA/kainite receptors (10 μM NBQX, Alomone Labs). ..

    Article Title: Synaptic drive onto inhibitory and excitatory principal neurons of the mouse lateral superior olive
    Article Snippet: Cells were held at −70 mV during synaptic data collection. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated glutamatergic inputs were isolated by bath-applied pharmacological blockade of glycine receptors (1 μM strychnine, Sigma), GABAA receptors (2 μM gabazine, Sigma), GABAB receptors (1 μM CGP55845, Sigma), and NMDA receptors (25 μM D-AP5, Alomone Labs). .. Glycine receptor (GlyR)-mediated inhibition was isolated by bath-applied pharmacological blockade of GABAA receptors (2 μM gabazine, Sigma), GABAB receptors (1 μMCGP55845, Sigma), NMDA receptors (25 μM D-AP5, Alomone Labs), and AMPA/kainite receptors (10 μM NBQX, Alomone Labs). ..

    Saline:

    Article Title: Repeated administration of AMPA/kainate antagonists transiently suppresses nicotine-seeking in an operant rat model.
    Article Snippet: .. NBQX was purchased from Alomone Labs (Jerusalem, Israel) and dissolved in saline to obtain 5 mg/ml concentration. ..

    other:

    Article Title: Crosstalk of noradrenergic Ca 2+ and cAMP signaling in astrocytes of the murine olfactory bulb
    Article Snippet: The following drugs were used: (R)-(−)-Phenylephrine hydrochloride (3-[(1R)-1-hydroxy-2-(methylamino)ethyl]phenol;hydrochloride; Abcam, Cambridge, United Kingdom), 2-APB (2-diphenylboranyloxyethanamine; Calbiochem, Darmstadt, Germany), BMY7378 (8-[2-[4-(2-methoxyphenyl)piperazin-1-yl]ethyl]-8-azaspiro[4.5]decane-7,9-dione;dihydrochloride; Hello Bio, Bristol, United Kingdom), CGP 55845 hydrochloride (benzyl-[(2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino]-2-hydroxypropyl]phosphinic acid; hydrochloride; Hello Bio), Cyclopiazonic acid ((2R,3S,9R)-5-acetyl-4-hydroxy-8,8-dimethyl-7,16-diazapentacyclo[9.6.1.02,9.03,7.015,18]octadeca-1(17),4,11(18),12,14-pentaen-6-one; Hello Bio), D-AP5 ((2R)-2-amino-5-phosphonopentanoic acid; Alomone Labs, Jerusalem, Israel), Norepinephrine bitartrate (4-[(1 R )-2-amino-1-hydroxyethyl]benzene-1,2-diol;2,3-dihydroxybutanedioic acid; Merck KGaA, Darmstadt, Germany), Forskolin ([(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-5,6,6a,8,9,10-hexahydro-2H-benzo[f]chromen-5-yl] acetate; Cayman Chemical, Ann Arbor, Michigan, USA), Gabazine (4-[6-imino-3-(4-methoxyphenyl)pyridazin-1-yl]butanoic acid; Abcam), HC-067047 (2-methyl-1-(3-morpholin-4-ylpropyl)-5-phenyl-N-[3-(trifluoromethyl)phenyl]pyrrole-3-carboxamide; Calbiochem), ICI 118,551 hydrochloride ((2R,3S)-1-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]-3-(propan-2-ylamino)butan-2-ol;hydrochloride; Tocris Bioscience, Bristol, United Kingdom), Isoprenaline hydrochloride (4-[1-hydroxy-2-(propan-2-ylamino)ethyl]benzene-1,2-diol;hydrochloride; Abcam), NBQX (disodium;6-nitro-7-sulfamoylbenzo[f]quinoxaline-2,3-diolate; Alomone Labs), NKY80 (2-amino-7-(furan-2-yl)-7,8-dihydro-6H-quinazolin-5-one; Tocris Bioscience), Prazosin hydrochloride ([4-(4-amino-6,7-dimethoxyquinazolin-2-yl)piperazin-1-yl]-(furan-2-yl)methanone;hydrochloride; Tocris Bioscience), Rauwolscine hydrochloride (methyl (1S,15S,18S,19S,20S)-18-hydroxy-1,3,11,12,14,15,16,17,18,19,20,21-dodecahydroyohimban-19-carboxylate;hydrochloride; Tocris Bioscience), RS100329 hydochloride (5-methyl-3-[3-[4-[2-(2,2,2-trifluoroethoxy)phenyl]piperazin-1-yl]propyl]-1H-pyrimidine-2,4-dione;hydrochloride; Tocris Bioscience), ST 034307 (6-chloro-2-(trichloromethyl)chromen-4-one; Tocris Bioscience), tetrodotoxin citrate ((1R,5R,6R,7R,9S,11S,12S,13S,14S)-3-amino-14-(hydroxymethyl)-8,10-dioxa-2,4-diazatetracyclo[7.3.1.17,11.01,6]tetradec-3-ene-5,9,12,13,14-pentol; Hello Bio), Xylazine (N-(2,6-dimethylphenyl)-5,6-dihydro-4H-1,3-thiazin-2-amine;hydrochloride; Merck).



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    Image Search Results


    Quantification of reactive oxygen species (ROS) induced by blue light irradiation. A549 cells were pretreated for 1 h with the indicated concentrations of Ir­(III) complexes. Cells were then irradiated for 1 h with blue light. Intracellular ROS were quantified using the CellRox Green probe and analyzed via flow cytometry. ROS levels are normalized to irradiated, untreated control samples. Data represent the mean ± standard deviation from three independent experiments, with duplicate samples in each run.

    Journal: Journal of Medicinal Chemistry

    Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

    doi: 10.1021/acs.jmedchem.5c03280

    Figure Lengend Snippet: Quantification of reactive oxygen species (ROS) induced by blue light irradiation. A549 cells were pretreated for 1 h with the indicated concentrations of Ir­(III) complexes. Cells were then irradiated for 1 h with blue light. Intracellular ROS were quantified using the CellRox Green probe and analyzed via flow cytometry. ROS levels are normalized to irradiated, untreated control samples. Data represent the mean ± standard deviation from three independent experiments, with duplicate samples in each run.

    Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

    Techniques: Irradiation, Flow Cytometry, Control, Standard Deviation

    Intracellular colocalization studies of iridium complexes 4 and 5 in A549 cells. A549 cells were incubated with 5 μM iridium complexes for 2 h, followed by costaining with specific cell trackers for mitochondria, endoplasmic reticulum, and lysosomes. Confocal microscopy was used for imaging. Scale bars represent 20 μm.

    Journal: Journal of Medicinal Chemistry

    Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

    doi: 10.1021/acs.jmedchem.5c03280

    Figure Lengend Snippet: Intracellular colocalization studies of iridium complexes 4 and 5 in A549 cells. A549 cells were incubated with 5 μM iridium complexes for 2 h, followed by costaining with specific cell trackers for mitochondria, endoplasmic reticulum, and lysosomes. Confocal microscopy was used for imaging. Scale bars represent 20 μm.

    Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

    Techniques: Incubation, Confocal Microscopy, Imaging

    Intracellular NADH depletion in A549 cells. A549 cells were treated with the respective complexes at their IC 50 concentrations for 2 h (1 h incubation in the dark followed by 1 h irradiation with blue light, 420 nm, 58 W/m 2 ). Experiments were performed in duplicate with triplicate samples per condition. Data are shown as mean ± SD. Statistical significance between dark-incubated and irradiated samples was assessed using a two-tailed t test. Significance levels are indicated as follows: * P ≤ 0.05, ** P ≤ 0.01; “ns” denotes a nonsignificant difference.

    Journal: Journal of Medicinal Chemistry

    Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

    doi: 10.1021/acs.jmedchem.5c03280

    Figure Lengend Snippet: Intracellular NADH depletion in A549 cells. A549 cells were treated with the respective complexes at their IC 50 concentrations for 2 h (1 h incubation in the dark followed by 1 h irradiation with blue light, 420 nm, 58 W/m 2 ). Experiments were performed in duplicate with triplicate samples per condition. Data are shown as mean ± SD. Statistical significance between dark-incubated and irradiated samples was assessed using a two-tailed t test. Significance levels are indicated as follows: * P ≤ 0.05, ** P ≤ 0.01; “ns” denotes a nonsignificant difference.

    Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

    Techniques: Incubation, Irradiation, Two Tailed Test