paper n a a549 ruvbl2 ko  (ATCC)

Journal: Journal of Medicinal Chemistry

Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

doi: 10.1021/acs.jmedchem.5c03280

Figure Lengend Snippet: Quantification of reactive oxygen species (ROS) induced by blue light irradiation. A549 cells were pretreated for 1 h with the indicated concentrations of Ir­(III) complexes. Cells were then irradiated for 1 h with blue light. Intracellular ROS were quantified using the CellRox Green probe and analyzed via flow cytometry. ROS levels are normalized to irradiated, untreated control samples. Data represent the mean ± standard deviation from three independent experiments, with duplicate samples in each run.

Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

Techniques: Irradiation, Flow Cytometry, Control, Standard Deviation

Journal: Journal of Medicinal Chemistry

Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

doi: 10.1021/acs.jmedchem.5c03280

Figure Lengend Snippet: Intracellular colocalization studies of iridium complexes 4 and 5 in A549 cells. A549 cells were incubated with 5 μM iridium complexes for 2 h, followed by costaining with specific cell trackers for mitochondria, endoplasmic reticulum, and lysosomes. Confocal microscopy was used for imaging. Scale bars represent 20 μm.

Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

Techniques: Incubation, Confocal Microscopy, Imaging

Journal: Journal of Medicinal Chemistry

Article Title: Novel 2‑(2′-Benzothiazolyl)-benzimidazole-Based Iridium(III) Photocatalysts Exhibit Antiproliferative Effects in 2D and 3D Cancer Cells to Bypass Hypoxia-Induced Resistance

doi: 10.1021/acs.jmedchem.5c03280

Figure Lengend Snippet: Intracellular NADH depletion in A549 cells. A549 cells were treated with the respective complexes at their IC 50 concentrations for 2 h (1 h incubation in the dark followed by 1 h irradiation with blue light, 420 nm, 58 W/m 2 ). Experiments were performed in duplicate with triplicate samples per condition. Data are shown as mean ± SD. Statistical significance between dark-incubated and irradiated samples was assessed using a two-tailed t test. Significance levels are indicated as follows: * P ≤ 0.05, ** P ≤ 0.01; “ns” denotes a nonsignificant difference.

Article Snippet: Elemental analysis (C, H, N, and S) of the ancillary ligand and the iridium (III) complexes was conducted in a TruSpec Leco microanalyzer equipped with nondispersive IR detectors for C, H, and S, and a thermal conductivity detector for N. A549 and HCC827 lung adenocarcinoma cell lines were from ATCC and colon carcinoma HCT-116 cells were from ECACC.

Techniques: Incubation, Irradiation, Two Tailed Test