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e15 5  (New England Biolabs)


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    Structured Review

    New England Biolabs e15 5
    E15 5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+kit/LunaScript+Multiplex+One-Step+RT-PCR+Kit/pmc03412014-52-76-78
    Average 94 stars, based on 26 article reviews
    e15 5 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Purification:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Amplification:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Multiplex Assay:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Article Title: NanoHIVSeq: A Long-Read Bioinformatics Pipeline for High-Throughput Processing of HIV Env Sequences
    Article Snippet: AM2616), and nuclease-free water was from ThermoFisher (Cat. #. .. J71786-AP), QIAamp viral RNA mini kit (Cat. No. 52904), RNaseOut (Invitrogen, Cat. No. 10777019), SuperScript III (Invitrogen, Cat. No. 18080085), Platinum Taq High Fidelity DNA polymerase (Invitrogen, Cat. No.11304029), ddPCR Super Mix for Probes (No dUTP): BioRad (#1863023), Droplets generated on BioRad DG32 auto generator with associated cartridges (#1864108) and oil for probes (#1863005), Master Mix for Env Droplet PCR: NEB LunaScript Multiplex One-Step RT-PCR kit (E1555L). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: Primers and qPCR probes of N1 (Centers for Disease Control and Prevention (CDC) primers and probe, Lu et al. 2020) and E (World Health Organization (WHO) primers and probe, Corman et al. 2020) genes were synthesized by Integrated DNA Technologies (IDT) and listed in Supplementary Table T1. .. Extracted RNA samples were reverse transcribed to cDNA and target amplicons were generated by multiplex PCR in a single step reaction using the LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. Primers for tiling amplicon library generation were designed using Primalscheme (Quick et al. 2017) and analyzed for primer dimers using Multiple Primer Analyzer (Thermo Fisher Scientific).

    Article Title: The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism
    Article Snippet: Contaminating genomic DNA was removed using the TURBO DNA-freeTM Kit (Invitrogen). .. RT-qPCR was performed with the LunaScript Multiplex One-Step RT-PCR Kit (NEB). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: The MagMax Microbiome Ultra Nucleic Acid Isolation kit (A42357) was purchased from Applied Biosystems (Foster City, CA). .. LunaScript Multiplex OneStep RT-PCR kit (E1555L) was purchased from New England Biolabs (NEB, Ipswich, USA). .. Custom primers were purchased from Invitrogen (Carlsbad, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Article Title: Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes
    Article Snippet: .. RNA was extracted from mixed-stage wild-type animals via Trizol and Zymo RNA Quick-RNA Miniprep kit according to manufacturer instructions (Zymo R1054), followed by RT-PCR using NEB Lunascript one-step RT-PCR kits (NEB E1555L). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: The MagMax Microbiome Ultra Nucleic Acid Isolation kit (A42357) was purchased from Applied Biosystems (Foster City, CA). .. LunaScript Multiplex OneStep RT-PCR kit (E1555L) was purchased from New England Biolabs (NEB, Ipswich, USA). .. Custom primers were purchased from Invitrogen (Carlsbad, USA).

    One Step RT-PCR:

    Article Title: Environmental air monitoring in international airports: A novel approach for enhanced pathogen surveillance
    Article Snippet: .. For targeted SARS-CoV-2 sequencing, purified total nucleic acid (TNA) was amplified by multiplex RT-PCR (NEB LunaScript Multiplex One-Step RT-PCR) using the ARTIC primer set (IDT ARTIC V5.3.2 NCOV-2019 Panel). .. Amplicons were pooled, and libraries prepared with a modified Illumina DNA Prep protocol.

    Article Title: NanoHIVSeq: A Long-Read Bioinformatics Pipeline for High-Throughput Processing of HIV Env Sequences
    Article Snippet: AM2616), and nuclease-free water was from ThermoFisher (Cat. #. .. J71786-AP), QIAamp viral RNA mini kit (Cat. No. 52904), RNaseOut (Invitrogen, Cat. No. 10777019), SuperScript III (Invitrogen, Cat. No. 18080085), Platinum Taq High Fidelity DNA polymerase (Invitrogen, Cat. No.11304029), ddPCR Super Mix for Probes (No dUTP): BioRad (#1863023), Droplets generated on BioRad DG32 auto generator with associated cartridges (#1864108) and oil for probes (#1863005), Master Mix for Env Droplet PCR: NEB LunaScript Multiplex One-Step RT-PCR kit (E1555L). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: Primers and qPCR probes of N1 (Centers for Disease Control and Prevention (CDC) primers and probe, Lu et al. 2020) and E (World Health Organization (WHO) primers and probe, Corman et al. 2020) genes were synthesized by Integrated DNA Technologies (IDT) and listed in Supplementary Table T1. .. Extracted RNA samples were reverse transcribed to cDNA and target amplicons were generated by multiplex PCR in a single step reaction using the LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. Primers for tiling amplicon library generation were designed using Primalscheme (Quick et al. 2017) and analyzed for primer dimers using Multiple Primer Analyzer (Thermo Fisher Scientific).

    Article Title: Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes
    Article Snippet: .. RNA was extracted from mixed-stage wild-type animals via Trizol and Zymo RNA Quick-RNA Miniprep kit according to manufacturer instructions (Zymo R1054), followed by RT-PCR using NEB Lunascript one-step RT-PCR kits (NEB E1555L). ..

    Article Title: The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism
    Article Snippet: Contaminating genomic DNA was removed using the TURBO DNA-freeTM Kit (Invitrogen). .. RT-qPCR was performed with the LunaScript Multiplex One-Step RT-PCR Kit (NEB). ..

    Generated:

    Article Title: NanoHIVSeq: A Long-Read Bioinformatics Pipeline for High-Throughput Processing of HIV Env Sequences
    Article Snippet: AM2616), and nuclease-free water was from ThermoFisher (Cat. #. .. J71786-AP), QIAamp viral RNA mini kit (Cat. No. 52904), RNaseOut (Invitrogen, Cat. No. 10777019), SuperScript III (Invitrogen, Cat. No. 18080085), Platinum Taq High Fidelity DNA polymerase (Invitrogen, Cat. No.11304029), ddPCR Super Mix for Probes (No dUTP): BioRad (#1863023), Droplets generated on BioRad DG32 auto generator with associated cartridges (#1864108) and oil for probes (#1863005), Master Mix for Env Droplet PCR: NEB LunaScript Multiplex One-Step RT-PCR kit (E1555L). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: Primers and qPCR probes of N1 (Centers for Disease Control and Prevention (CDC) primers and probe, Lu et al. 2020) and E (World Health Organization (WHO) primers and probe, Corman et al. 2020) genes were synthesized by Integrated DNA Technologies (IDT) and listed in Supplementary Table T1. .. Extracted RNA samples were reverse transcribed to cDNA and target amplicons were generated by multiplex PCR in a single step reaction using the LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. Primers for tiling amplicon library generation were designed using Primalscheme (Quick et al. 2017) and analyzed for primer dimers using Multiple Primer Analyzer (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: NanoHIVSeq: A Long-Read Bioinformatics Pipeline for High-Throughput Processing of HIV Env Sequences
    Article Snippet: AM2616), and nuclease-free water was from ThermoFisher (Cat. #. .. J71786-AP), QIAamp viral RNA mini kit (Cat. No. 52904), RNaseOut (Invitrogen, Cat. No. 10777019), SuperScript III (Invitrogen, Cat. No. 18080085), Platinum Taq High Fidelity DNA polymerase (Invitrogen, Cat. No.11304029), ddPCR Super Mix for Probes (No dUTP): BioRad (#1863023), Droplets generated on BioRad DG32 auto generator with associated cartridges (#1864108) and oil for probes (#1863005), Master Mix for Env Droplet PCR: NEB LunaScript Multiplex One-Step RT-PCR kit (E1555L). ..

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: Primers and qPCR probes of N1 (Centers for Disease Control and Prevention (CDC) primers and probe, Lu et al. 2020) and E (World Health Organization (WHO) primers and probe, Corman et al. 2020) genes were synthesized by Integrated DNA Technologies (IDT) and listed in Supplementary Table T1. .. Extracted RNA samples were reverse transcribed to cDNA and target amplicons were generated by multiplex PCR in a single step reaction using the LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. Primers for tiling amplicon library generation were designed using Primalscheme (Quick et al. 2017) and analyzed for primer dimers using Multiple Primer Analyzer (Thermo Fisher Scientific).

    Reverse Transcription:

    Article Title: Wastewater surveillance of SARS-CoV-2 variants to track community outbreaks in Hong Kong
    Article Snippet: Primers and qPCR probes of N1 (Centers for Disease Control and Prevention (CDC) primers and probe, Lu et al. 2020) and E (World Health Organization (WHO) primers and probe, Corman et al. 2020) genes were synthesized by Integrated DNA Technologies (IDT) and listed in Supplementary Table T1. .. Extracted RNA samples were reverse transcribed to cDNA and target amplicons were generated by multiplex PCR in a single step reaction using the LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. Primers for tiling amplicon library generation were designed using Primalscheme (Quick et al. 2017) and analyzed for primer dimers using Multiple Primer Analyzer (Thermo Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism
    Article Snippet: Contaminating genomic DNA was removed using the TURBO DNA-freeTM Kit (Invitrogen). .. RT-qPCR was performed with the LunaScript Multiplex One-Step RT-PCR Kit (NEB). ..



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