mouse monoclonal anti trf2 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mouse monoclonal anti trf2

a The diagram shows the workflow on a timeline: on the day after cell seeding, cells were treated with RHPS4 (0.2 and/or 0.5 μM). At 48 h after treatment, cells were again treated with RHPS4 (0.2 and/or 0.5 μM) or harvested and counted. At 72 h after the first treatment, the cell viability was checked, and finally at 96 h after the first treatment, the cells were harvested and processed. b Representative images showing U251MG cells stained by immunofluorescence using anti-BLM (red signals), and anti-TRF1 <t>or</t> <t>anti-TRF2</t> (green signals) antibodies. Merged images allow visualization of colocalizing dots (yellow signals). Yellow arrows indicate BLM and TRF1-TRF2 colocalizations. c Quantification of the colocalizations between BLM and TRF1 or TRF2 proteins in U251MG cell line upon RHPS4 treatment (0.2 and 0.5 μM). d , e Sensitivity of U251MG and BLM −/− cell lines to RHPS4 concentrations ranging from 0.01 to 2 μM (0.01; 0.125; 0.25; 0.5; 1; 2 μM), evaluated 96 h after the first treatment. Mitomycin C (MMC) was used as a positive control at concentrations of 0.1; 0.5; 1; 2; 5 μg/ml. The Sulforhodamine B (SRB) cytotoxicity assay showed that RHPS4 sensitivity was unchanged in U251MG and BLM −/− (IC50 was 0.56 μM in both cell lines). f Short-term cell proliferation in untreated cells, U251MG and BLM −/− , and in RHPS4-treated cells (0.5 μM) as evaluated 48 and 96 h after the first treatment. g Long-term cell proliferation in U251MG and BLM −/− untreated and RHPS4-treated cells (0.5 μM). Scale bars represent 5 μm. * p < 0.05, ** p < 0.01 (two-way ANOVA; n = 3). Error bars denote the standard deviation of the mean.

Mouse Monoclonal Anti Trf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti-trf2/TRF2+Antibody/pmc12830668-280-42-48
Average 93 stars, based on 166 article reviews

mouse monoclonal anti trf2 - by Bioz Stars, 2026-10

93/100 stars

Images

1) Product Images from "BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress"

Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress

Journal: Communications Biology

doi: 10.1038/s42003-025-09367-z

Figure Legend Snippet: a The diagram shows the workflow on a timeline: on the day after cell seeding, cells were treated with RHPS4 (0.2 and/or 0.5 μM). At 48 h after treatment, cells were again treated with RHPS4 (0.2 and/or 0.5 μM) or harvested and counted. At 72 h after the first treatment, the cell viability was checked, and finally at 96 h after the first treatment, the cells were harvested and processed. b Representative images showing U251MG cells stained by immunofluorescence using anti-BLM (red signals), and anti-TRF1 or anti-TRF2 (green signals) antibodies. Merged images allow visualization of colocalizing dots (yellow signals). Yellow arrows indicate BLM and TRF1-TRF2 colocalizations. c Quantification of the colocalizations between BLM and TRF1 or TRF2 proteins in U251MG cell line upon RHPS4 treatment (0.2 and 0.5 μM). d , e Sensitivity of U251MG and BLM −/− cell lines to RHPS4 concentrations ranging from 0.01 to 2 μM (0.01; 0.125; 0.25; 0.5; 1; 2 μM), evaluated 96 h after the first treatment. Mitomycin C (MMC) was used as a positive control at concentrations of 0.1; 0.5; 1; 2; 5 μg/ml. The Sulforhodamine B (SRB) cytotoxicity assay showed that RHPS4 sensitivity was unchanged in U251MG and BLM −/− (IC50 was 0.56 μM in both cell lines). f Short-term cell proliferation in untreated cells, U251MG and BLM −/− , and in RHPS4-treated cells (0.5 μM) as evaluated 48 and 96 h after the first treatment. g Long-term cell proliferation in U251MG and BLM −/− untreated and RHPS4-treated cells (0.5 μM). Scale bars represent 5 μm. * p < 0.05, ** p < 0.01 (two-way ANOVA; n = 3). Error bars denote the standard deviation of the mean.

Techniques Used: Staining, Immunofluorescence, Positive Control, Cytotoxicity Assay, Standard Deviation

Incubation:

Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress
Article Snippet: .. The slides were then incubated in blocking buffer (1% BSA dissolved in 1X PBS [w/v]) and then incubated overnight (ON) at 4 °C with the primary antibodies (rabbit polyclonal anti-BLM (#A300-110A, Bethyl) (1:100); mouse monoclonal anti-TRF1 (4E4 clone, GTX70304, GeneTex) (1:20) or mouse monoclonal anti-TRF2 (9F10 clone, sc-47693, Santa Cruz Biotechnology) (1:100)). .. The day after, the slides were washed three times in 1% BSA and then incubated with secondary antibodies (Alexa Fluor 488 goat anti-mouse (Invitrogen) and Alexa Fluor 546 goat anti-rabbit (Invitrogen)) diluted in blocking buffer.

Article Title: TRF2 Non-Telomeric Function is Indispensable for Neural stemness
Article Snippet: .. After blocking, cells were treated with the appropriate antibodies: TRF2 Santa Cruz #sc52968, β-III Tubulin abcam # ab18207, PAX6 abcam #ab78545, 53BP1 abcam #ab36823 and incubated overnight at 4°C in a humid chamber. ..

Article Title: TRF2 Non-Telomeric Function is Indispensable for Neural stemness
Article Snippet: .. Primary antibodies (Ki67 abcam#ab15580, γH2AX CST #S139 (20E3), 53BP1 abcam #ab36823, p-ATM CST #S1981(D25E5), MAP2 abcam #ab32454, BDNF abcam #ab108319, L1CAM abcam #ab208155, and TRF2 Santa Cruz #sc52968/ Merck #4A794)were diluted in 1% BSA (in PBS) at a 1:250 volume ratio and incubated with the cells as a cocktail for 2 hours at room temperature. ..

Article Title: Sex differential effects of early maternal separation on PTSD susceptibility in adult rats accompanied by telomere shortening in the hippocampus.
Article Snippet: Early life stress (ELS) is thought to be a leading cause of mental disorders in adulthood, including PTSD.. Recent studies have found that such stress has a gender and resilient specific effect on adult PTSD.. This study aimed to assess emotion, and cognitive behavior, and to examine the sex differences and resilience of ELS on adult PTSD.

Blocking Assay:

other:

Article Title: Apurinic/apyrimidinic endodeoxyribonuclease 1 contributes to the repair of damaged intercalated-motif of telomeric sequences
Article Snippet: The membrane was colored with Revert 700 Staining (926-11010, Li-Cor Biosciences) and acquired with Odyssey CLx scanner/ImageStudio Software (Li-Cor Biosciences).

Article Title: Apurinic/apyrimidinic endodeoxyribonuclease 1 contributes to the repair of damaged intercalated-motif of telomeric sequences
Article Snippet: Next, cells were incubated with the secondary antibody (Alexa Fluor 488, 111-545-003, Jackson ImmunoResearch; Alexa Fluor 633, A211050, A-21071, Invitrogen; Alexa Fluor 555, ab150114, Abcam), diluted in FBS 10% in WBA for 1 hour at RT.

Ubiquitin Proteomics:

Article Title: UBE2D3 facilitates NHEJ by orchestrating ATM signalling through multi-level control of RNF168.
Article Snippet: Horseradish peroxidase (HRP)-conjugated secondary antibodies were used for detection by enhanced chemiluminescence (Supersignal and Supersignal West Pico Plus, Thermo Fisher Scientific), either on film (Amersham Hyperfilm MP), on a Chemidoc XRS + 4.0.1 (Bio-Rad) or a G: box mini Syngene (with Genesys v1.6.9.0 software). .. Primary antibodies used were against UBE2D3 (Y-25, sc-100618, SCBT, 1:500; 11677-1-AP, Proteintech, 1:500; 4330S, CST, 1:500 and A615, Boston Biochem, 1:2000), KAP1 (22553, Abcam, 1:1000), phospho-Kap1 S824 (A300 767A, Bethyl, 1:1000), 53BP1 (NB100-305, Novus, 1:500 and A300-272A, Bethyl, 1:2000), phospho-ATMS1981 (4526, CST, 1:1000), phospho-H2AX S139 (5636, Millipore, 1:1000), CHK2 (611570, BD, 1:500), c-myc (9E10, sc-40, SCBT, 1:250), HA (MMS101R, Covance, 1:1000), TRF2 (NB110-57130, Novus, 1:500), RNF8 (sc133971, SCBT, 1:250), MAD2L2 (14, sc-135977, SCBT, 1:500), GFP IgG fraction (A11122, Thermo Fisher Scientific, 1:1000), FLAG M2 (F1804, Sigma-Aldrich, 1:1000), Histone H3 (ab1791, Abcam, 1:10,000), hRNF168 (ABE367, Millipore, 1:500), hRNF168 (ABE467, Merck-Millipore, 1:1000), mRnf168 (gift from D. Durocher, 1:1000), hRIF1 (A300569A, Bethyl, 1:1000), mRIF1 (gift from S. Boulton and R. Chapman, 1:1000), Ligase 4 (H-300, sc-28232, SCBT, 1:300; NB110-57379, Novus, 1:500), FK2 (04-263, Millipore, 1:2000), HP1α (2616S, CST, 1:1000), Ubiquitin (P4D1, sc-8017, SCBT, 1:1000), HDAC1 (PA1-860, Thermo Fisher Scientific, 1:1000), PP2A C subunit, clone 1D6 antibody (05-421, Sigma-Aldrich/Millipore, 1:500), CDK4 (C-22, sc-260, SCBT, 1:500), HSP90 α/β (H-114, sc-7947, SCBT, 1:1000), γ-tubulin (T6557, SigmaAldrich, 1:10,000) β-actin (A5316, Sigma-Aldrich, 1:10,000), β-catenin (610154, BD, 1:10,000) and GAPDH (PA1-987, Thermo Fisher Scientific, 1:1000).HSP90α/β, γ-tubulin,β-actin,β-catenin andGAPDHwere used for loading controls. ..

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