Review



mouse monoclonal anti trf2 antibody  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology mouse monoclonal anti trf2 antibody
    Fig. 4 <t>TRF2</t> foci quantification detected by immunofluorescence on A2780 and A2780cis cells treated with cisplatin, Pt-ttpy, and Pt-tpy for 96 h at their respective IC80 concentration. (A) A2780 cells were processed for immunofluorescence using antibodies against TRF2. (B) % of TRF2 foci after A2780 cell treatments with cisplatin, Pt-ttpy, and Pt-tpy. (C) % of TRF2 foci after A2780cis cell treatments with cisplatin, Pt-ttpy, and Pt-tpy (mean of at least three experiments). ** Indicates a Mann and Withney test P-value < 0.01 and * P < 0.05 (GraphPad PRISM software).
    Mouse Monoclonal Anti Trf2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+mab+anti-trf2+clone+4a794/pm34021581-60-31-46?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 165 article reviews
    mouse monoclonal anti trf2 antibody - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage."

    Article Title: Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage.

    Journal: Metallomics : integrated biometal science

    doi: 10.1093/mtomcs/mfab029

    Fig. 4 TRF2 foci quantification detected by immunofluorescence on A2780 and A2780cis cells treated with cisplatin, Pt-ttpy, and Pt-tpy for 96 h at their respective IC80 concentration. (A) A2780 cells were processed for immunofluorescence using antibodies against TRF2. (B) % of TRF2 foci after A2780 cell treatments with cisplatin, Pt-ttpy, and Pt-tpy. (C) % of TRF2 foci after A2780cis cell treatments with cisplatin, Pt-ttpy, and Pt-tpy (mean of at least three experiments). ** Indicates a Mann and Withney test P-value < 0.01 and * P < 0.05 (GraphPad PRISM software).
    Figure Legend Snippet: Fig. 4 TRF2 foci quantification detected by immunofluorescence on A2780 and A2780cis cells treated with cisplatin, Pt-ttpy, and Pt-tpy for 96 h at their respective IC80 concentration. (A) A2780 cells were processed for immunofluorescence using antibodies against TRF2. (B) % of TRF2 foci after A2780 cell treatments with cisplatin, Pt-ttpy, and Pt-tpy. (C) % of TRF2 foci after A2780cis cell treatments with cisplatin, Pt-ttpy, and Pt-tpy (mean of at least three experiments). ** Indicates a Mann and Withney test P-value < 0.01 and * P < 0.05 (GraphPad PRISM software).

    Techniques Used: Immunofluorescence, Concentration Assay, Software

    Fig. 5 Proteins bound to telomeres quantified from dot blots. Dot blot membranes of ChIP of TRF2, TRF1, POT1, and H3 on A2780 cells treated by doses of Pt-ttpy (A), Pt-tpy (B), and cisplatin (C) for 96 h at their respective IC80 concentration. (D) % of proteins bound to telomeres of A2780 cells. (E) % of proteins bound to telomeres of A2780cis cells. Telomeric sequences were evidenced in a DNA fraction immunoprecipitated by an anti-TRF1, anti-TRF2, anti-H3 antibody using a 32P radio labelled 800pb telomeric probe and normalized with α 32P radio labelled Alu sequences in untreated, cisplatin, Pt-ttpy, and Pt-tpy treated cells. 200 ng of DNA were blotted for each sample. The % represents the quantitative values of telomeric DNA signals in the samples originating from cells with treatment compared to the cells without any treatment. Quantitative values of telomeric DNA signals are calculated as the ratio between telomeric DNA signal precipitation and telomeric DNA signals in the INPUT for the same amount of blotted DNA. These values have been normalized by the amount of blotted DNA for each sample quantified by the non-specific Alu probe, following the formula: (telomere IP/telomere INPUT)/(Alu IP/Alu INPUT). (Means of at least three experiments) ** Indicates a Mann and Withney test P-value < 0.01 and * P < 0.05 (GraphPad PRISM software). Statistical analysis was made by comparing the amount of protein bound to telomeres for each treatment.
    Figure Legend Snippet: Fig. 5 Proteins bound to telomeres quantified from dot blots. Dot blot membranes of ChIP of TRF2, TRF1, POT1, and H3 on A2780 cells treated by doses of Pt-ttpy (A), Pt-tpy (B), and cisplatin (C) for 96 h at their respective IC80 concentration. (D) % of proteins bound to telomeres of A2780 cells. (E) % of proteins bound to telomeres of A2780cis cells. Telomeric sequences were evidenced in a DNA fraction immunoprecipitated by an anti-TRF1, anti-TRF2, anti-H3 antibody using a 32P radio labelled 800pb telomeric probe and normalized with α 32P radio labelled Alu sequences in untreated, cisplatin, Pt-ttpy, and Pt-tpy treated cells. 200 ng of DNA were blotted for each sample. The % represents the quantitative values of telomeric DNA signals in the samples originating from cells with treatment compared to the cells without any treatment. Quantitative values of telomeric DNA signals are calculated as the ratio between telomeric DNA signal precipitation and telomeric DNA signals in the INPUT for the same amount of blotted DNA. These values have been normalized by the amount of blotted DNA for each sample quantified by the non-specific Alu probe, following the formula: (telomere IP/telomere INPUT)/(Alu IP/Alu INPUT). (Means of at least three experiments) ** Indicates a Mann and Withney test P-value < 0.01 and * P < 0.05 (GraphPad PRISM software). Statistical analysis was made by comparing the amount of protein bound to telomeres for each treatment.

    Techniques Used: Dot Blot, Concentration Assay, Immunoprecipitation, Software

    Fig. 6 Western blot of TRF2, pTRF2, and telomere length from A2780 treated cells with cisplatin, Pt-ttpy, and Pt-tpy for 96 h. (A) Western blot of TRF2 membranes, (B) TRF2 relative protein level normalized with actin (Mean of three experiments), (C) Western blot of pTRF2 membranes for 50 μM 8 h treatment, and 85 mM etoposide as positive control (D) Southern blot or (E) Relative telomere length measured by qPCR. The values were normalized using 36B4u gene (mean of three experiments).
    Figure Legend Snippet: Fig. 6 Western blot of TRF2, pTRF2, and telomere length from A2780 treated cells with cisplatin, Pt-ttpy, and Pt-tpy for 96 h. (A) Western blot of TRF2 membranes, (B) TRF2 relative protein level normalized with actin (Mean of three experiments), (C) Western blot of pTRF2 membranes for 50 μM 8 h treatment, and 85 mM etoposide as positive control (D) Southern blot or (E) Relative telomere length measured by qPCR. The values were normalized using 36B4u gene (mean of three experiments).

    Techniques Used: Western Blot, Positive Control, Southern Blot



    Similar Products

    mouse mab anti-trf2 clone 4a794  (Millipore)
    90
    Millipore mouse mab anti-trf2 clone 4a794
    Mouse Mab Anti Trf2 Clone 4a794, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+mab+anti-trf2+clone+4a794/pmc08537501-309-8-13?v=Millipore
    Average 90 stars, based on 1 article reviews
    mouse mab anti-trf2 clone 4a794 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results