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minion min-101b sequencer  (Oxford Nanopore)

 
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    Oxford Nanopore minion min-101b sequencer
    Minion Min 101b Sequencer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/min-101b/minion+device/pm39907824-74-8-10
    Average 90 stars, based on 1 article reviews
    minion min-101b sequencer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title:
    Article Snippet: Conventional RNA sequencing of iPSC lines R24-26 against their respective starting material and hESCs was carried out at Alacris Theranostics using Illumina instrumentation and standard bioinformatics analysis tools. .. RNA-seq of undifferentiated R26 iPSCs against differentiated progeny was performed using Oxford Nanopore MinION R9 flowcells (Oxford Nanopore # FLO-MIN106D) on a corresponding MinION device. ..

    Nanopore Sequencing:

    Article Title: Wastewater-Based Surveillance of Human Adenoviruses in Italy: Quantification by Digital PCR and Molecular Typing via Nanopore Amplicon Sequencing.
    Article Snippet: .. In brief, nanopore sequencing was conducted on a MinION device (Oxford Nanopore Technologies, Oxford, UK), using the cDNA-PCR Sequencing Kit (SQK-PCS109) along with native barcodes, as per the manufacturer’s instructions. ..

    Sequencing:

    Article Title: Wastewater-Based Surveillance of Human Adenoviruses in Italy: Quantification by Digital PCR and Molecular Typing via Nanopore Amplicon Sequencing.
    Article Snippet: .. In brief, nanopore sequencing was conducted on a MinION device (Oxford Nanopore Technologies, Oxford, UK), using the cDNA-PCR Sequencing Kit (SQK-PCS109) along with native barcodes, as per the manufacturer’s instructions. ..

    Article Title: An emerging Platynota sp. (Lepidoptera: Tortricidae) infesting blueberry ( Vaccinium corymbosum ) in the central coast of Peru
    Article Snippet: The PCR library was prepared using Oxford Nanopore Technologies (ONT) sequencing (SQK-LSK110) and barcode kits (EXP-NBD196), following manufacturer’s protocols. .. The final library was loaded onto a Flongle flow cell (FLO-FLG110) and run for 24 h. A total DNA library was prepared with the same sequencing kit, loaded onto a R9.4.1 flow cell (FLO-MIN106) and run for 72 h. Sequencing was conducted on the MinION device (Oxford Nanopore Technologies, UK) using the ONT MinKNOW Software. ..

    Article Title: Allelism of Rps3b and Rps11 revealed by NLR gene capture of resistance genes to Phytophthora sojae in soybean.
    Article Snippet: .. 2.6.4 Whole-genome sequencing and synteny analysis Soybean accessions PI 591509 (Rps3b), PI 172901 (Rps3b), L88-1479 (Rps3b), and Haro33 (Rps3b) were sequenced on a MinIon device from Oxford Nanopore Technologies. ..

    Article Title: Rapid identification of a Serratia marcescens outbreak in a neonatal intensive care unit by third-generation long-read nanopore sequencing.
    Article Snippet: Final DNA concentration was measured using a QubitTM dsDNA HS Assay Kit on Qubit® 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Whole genome sequencing by nanopore and quality control WGS of S. marcescens isolates was performed by the Oxford Nanopore Technologies (ONT) MinION device according to the manufacturer’s instructions. ..

    Article Title: Changes in the Anopheles arabiensis transcriptome and gut microbiota profiles associated with Microsporidia MB
    Article Snippet: The purity of both RNA and DNA was determined using NanoDrop One/Onec Microvolume UV-Vis Spectrophotometer (ThermoFischer Scientific, Waltham, Massachusetts, United States), while quantification of both RNA and DNA was done using QubitTM 4 Fluorometer (Invitrogen, Waltham, Massachusetts, United States) throughout the entire study. .. For each sample, 50 ng of the extracted RNA was prepared for sequencing using the Oxford Nanopore Technology (ONT) MinION device. ..

    Software:

    Article Title: An emerging Platynota sp. (Lepidoptera: Tortricidae) infesting blueberry ( Vaccinium corymbosum ) in the central coast of Peru
    Article Snippet: The PCR library was prepared using Oxford Nanopore Technologies (ONT) sequencing (SQK-LSK110) and barcode kits (EXP-NBD196), following manufacturer’s protocols. .. The final library was loaded onto a Flongle flow cell (FLO-FLG110) and run for 24 h. A total DNA library was prepared with the same sequencing kit, loaded onto a R9.4.1 flow cell (FLO-MIN106) and run for 72 h. Sequencing was conducted on the MinION device (Oxford Nanopore Technologies, UK) using the ONT MinKNOW Software. ..

    Control:

    Article Title: Rapid identification of a Serratia marcescens outbreak in a neonatal intensive care unit by third-generation long-read nanopore sequencing.
    Article Snippet: Final DNA concentration was measured using a QubitTM dsDNA HS Assay Kit on Qubit® 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Whole genome sequencing by nanopore and quality control WGS of S. marcescens isolates was performed by the Oxford Nanopore Technologies (ONT) MinION device according to the manufacturer’s instructions. ..

    Whole Complete genome sequencing:

    Article Title: Rapid identification of a Serratia marcescens outbreak in a neonatal intensive care unit by third-generation long-read nanopore sequencing.
    Article Snippet: Final DNA concentration was measured using a QubitTM dsDNA HS Assay Kit on Qubit® 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Whole genome sequencing by nanopore and quality control WGS of S. marcescens isolates was performed by the Oxford Nanopore Technologies (ONT) MinION device according to the manufacturer’s instructions. ..



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    ( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
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    ( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
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    Image Search Results


    ( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore sequencing using v10 chemistry native barcoding and sequenced on a MinION sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.

    Journal: bioRxiv

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples

    doi: 10.1101/2025.04.03.647077

    Figure Lengend Snippet: ( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore sequencing using v10 chemistry native barcoding and sequenced on a MinION sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.

    Article Snippet: MinION Mk1B Sequencing Device (MIN-101B), Ligation Sequencing Kit (SQK-LSK109) with the Adapter Mix II Expansion (EXP-AMII001), Spot-ON Flow Cell, R9 Version (FLO-MIN106D), MinION Flow Cell, R10 Version (FLO-MIN114), Flongle Flow Cell, R10 Version (FLO-FLG114), Flongle Sequencing Expansion (EXP-FSE002), and the Native Barcoding Kit 96 v14 (SQK-NBD114.96) were obtained from Oxford Nanopore Technologies (Oxford, United Kingdom). dPTP (6H,8H-3,4-Dihydro-pyrimido(4,5-c)(1,2)oxazin-7-one-8-β-D-2’-deoxy-ribofuranoside-5’-triphosphate, Sodium salt) (NU-1119S) was obtained from Jena Bioscience (Jena, Germany).

    Techniques: Binding Assay, Amplification, Nanopore Sequencing, Isolation, Sequencing, Plasmid Preparation, Labeling