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150 penicilin strreptomycin wisent 450 201 el zeocin thermofisher r25001 blasticidin bioshop bla477 25 pcdna  (Thermo Fisher)


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    Thermo Fisher 150 penicilin strreptomycin wisent 450 201 el zeocin thermofisher r25001 blasticidin bioshop bla477 25 pcdna
    150 Penicilin Strreptomycin Wisent 450 201 El Zeocin Thermofisher R25001 Blasticidin Bioshop Bla477 25 Pcdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/md-201/Hygromycin+B/pm32814032-311-69-74
    Average 99 stars, based on 1 article reviews
    150 penicilin strreptomycin wisent 450 201 el zeocin thermofisher r25001 blasticidin bioshop bla477 25 pcdna - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Human dental pulp stem cells grafted into C57BL/6J hippocampus differentiate towards immature neuronal like cells displaying action potential firing activity
    Article Snippet: .. Cells were infected with the pLenti-CMV-GFP-Hygro vector (656–4) (Addgene viral prep # 17446-LV), kindly provided by Eric Campeu and Paul Kaufman, which confers resistance to hygromycin-B antibiotic (#31282-04-9, Thermo Fisher) and was purchased from the addgene non-profit repository ( ). ..

    Article Title: A primordial synaptotagmin-independent function of complexin in regulated exocytosis.
    Article Snippet: .. After lentiviral infection, cells were sequentially selected using 1 μg/mL puromycin (Sigma-Aldrich, #P8833) and 500 μg/mL hygromycin B (Thermo Fisher Scientific, #10687010). .. After lentiviral infection, cells were sequentially selected using 1 μg/mL puromycin (Sigma-Aldrich, #P8833) and 500 μg/mL hygromycin B (Thermo Fisher Scientific, #10687010).

    Plasmid Preparation:

    Article Title: Human dental pulp stem cells grafted into C57BL/6J hippocampus differentiate towards immature neuronal like cells displaying action potential firing activity
    Article Snippet: .. Cells were infected with the pLenti-CMV-GFP-Hygro vector (656–4) (Addgene viral prep # 17446-LV), kindly provided by Eric Campeu and Paul Kaufman, which confers resistance to hygromycin-B antibiotic (#31282-04-9, Thermo Fisher) and was purchased from the addgene non-profit repository ( ). ..

    Recombinant:


    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies EMC3/TM111 Recombinant Polyclonal Antibody Invitrogen 711771; RRID: AB_2716909 Calnexin Enzo ADI-SPA-865; RRID: AB_11180747 Sec61α Gilmore lab Ref: 84 RRID: n/a Sec61β Hegde lab Ref: 85 RRID: n/a RPL8 Abcam ab169538 RRID: AB_2714187 FLAG-HRP Sigma A8592 RRID: AB_439702 CCDC47 Bethyl A305-100A RRID: AB_2631495 TMCO1 Invitrogen PA5-43350 RRID: AB_2576547 TMEM147 Invitrogen PA5-95876 RRID: AB_2807678 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch 111-035-003 RRID: AB_2313567 Chemicals, peptides, and recombinant proteins Bismaleimidohexane (BMH) Thermo 22330 TransIT 293 Mirus MIR 2704 m7G(5’)ppp(5’)G RNA Cap Structure Analog New England Biolabs S1404L RNasin® Ribonuclease Inhibitor Promega N2515 Amino acid kit Promega L9961/L996B SP6 Polymerase NEB M0207L Creatine kinase Roche CK-RO, SKU# 10127566001 Creatine phosphate Roche CRPHO-RO, SKU# 10621714001 Fetal bovine serum Gibco 10270106 Rabbit Reticulocyte Lysate Mix Green Hectares N/A EDTA VWR 20302.260 DMEM, high glucose, GlutaMAX, pyruvate Gibco 10569-010 FluoroBrite DMEM Gibco A18967-01 Hygromycin B Thermo Fisher 10687-010 SuperSignal West Pico Chemiluminescent substrate Thermo Fisher 34080 Doxycycline Sigma D9891 NEBuilder® HiFi DNA Assembly Master Mix NEB E2621L Sepharose, Strep-Tactin IBA IB212010102-1201-010 Concanavalin A (ConA) resin Sigma GE17-0440-03 LipofectamineTM RNAiMAX transfection reagent Invitrogen 13778075 PonceauS Solution Sigma P-7170-1L Digitonin Sigma 300410 DAPI Sigma D9542 ATP Roche ATPDS-RO, SKU#10519979001 UTP Sigma U6875 CTP Sigma C1506 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–16.e1–e5, August 6, 2026 e1 .. This work was supported by the UK Medical Research Council (grant MC_UP_A022_1007 to R.S.H.), MRC International PhD Programme (to L.S.), and a European Molecular Biology Organization Postdoctoral Fellowship (to H.W.).

    Protease Inhibitor:


    Magnetic Beads:


    Real-time Polymerase Chain Reaction:

    Article Title: Histidine to glutamine substitutants, a regulated process that impacts cell survival and suggests histidine shortage in cancer.
    Article Snippet: .. 24 h later, transduced cells were selected by addition of 2 μg/mL puromycin (Bio-connect), a range of 5-10 μg/mL blasticidin (Invivogen) to the medium or 100 μg/mL hygromycin B (GIBCO) to the medium. qPCR Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific) following the manufacturer’s instructions. ..

    Isolation:

    Article Title: Histidine to glutamine substitutants, a regulated process that impacts cell survival and suggests histidine shortage in cancer.
    Article Snippet: .. 24 h later, transduced cells were selected by addition of 2 μg/mL puromycin (Bio-connect), a range of 5-10 μg/mL blasticidin (Invivogen) to the medium or 100 μg/mL hygromycin B (GIBCO) to the medium. qPCR Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific) following the manufacturer’s instructions. ..

    Transfection:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies EMC3/TM111 Recombinant Polyclonal Antibody Invitrogen 711771; RRID: AB_2716909 Calnexin Enzo ADI-SPA-865; RRID: AB_11180747 Sec61α Gilmore lab Ref: 84 RRID: n/a Sec61β Hegde lab Ref: 85 RRID: n/a RPL8 Abcam ab169538 RRID: AB_2714187 FLAG-HRP Sigma A8592 RRID: AB_439702 CCDC47 Bethyl A305-100A RRID: AB_2631495 TMCO1 Invitrogen PA5-43350 RRID: AB_2576547 TMEM147 Invitrogen PA5-95876 RRID: AB_2807678 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch 111-035-003 RRID: AB_2313567 Chemicals, peptides, and recombinant proteins Bismaleimidohexane (BMH) Thermo 22330 TransIT 293 Mirus MIR 2704 m7G(5’)ppp(5’)G RNA Cap Structure Analog New England Biolabs S1404L RNasin® Ribonuclease Inhibitor Promega N2515 Amino acid kit Promega L9961/L996B SP6 Polymerase NEB M0207L Creatine kinase Roche CK-RO, SKU# 10127566001 Creatine phosphate Roche CRPHO-RO, SKU# 10621714001 Fetal bovine serum Gibco 10270106 Rabbit Reticulocyte Lysate Mix Green Hectares N/A EDTA VWR 20302.260 DMEM, high glucose, GlutaMAX, pyruvate Gibco 10569-010 FluoroBrite DMEM Gibco A18967-01 Hygromycin B Thermo Fisher 10687-010 SuperSignal West Pico Chemiluminescent substrate Thermo Fisher 34080 Doxycycline Sigma D9891 NEBuilder® HiFi DNA Assembly Master Mix NEB E2621L Sepharose, Strep-Tactin IBA IB212010102-1201-010 Concanavalin A (ConA) resin Sigma GE17-0440-03 LipofectamineTM RNAiMAX transfection reagent Invitrogen 13778075 PonceauS Solution Sigma P-7170-1L Digitonin Sigma 300410 DAPI Sigma D9542 ATP Roche ATPDS-RO, SKU#10519979001 UTP Sigma U6875 CTP Sigma C1506 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–16.e1–e5, August 6, 2026 e1 .. This work was supported by the UK Medical Research Council (grant MC_UP_A022_1007 to R.S.H.), MRC International PhD Programme (to L.S.), and a European Molecular Biology Organization Postdoctoral Fellowship (to H.W.).

    Milk:

    Article Title: Mutant huntingtin disrupts TET1 transcription and alters DNA methylation in a Huntington's disease knock-in pig model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IP/IF: Rabbit anti-HTT (EM48) serum Proteintech N/A WB: Anti-Polyglutamine-Expansion Diseases Marker (mouse monoclonal 5TF11C2) Millipore Cat# MAB1574; RRID: AB_94263 WB: Anti-Huntingtin (rabbit monoclonal, EPR5526) Abcam Cat# ab109115; RRID: AB_10863082 WB: Anti-Huntingtin (mouse monoclonal 1HU-4C8) Millipore Cat# MAB2166; RRID:AB_11213141 WB: Anti-Vinculin antibody (mouse monoclonal 7F9) Millipore Cat# MAB3574; RRID: AB_2304338 WB: Anti- beta Actin Santa Cruz Cat# sc-47778; RRID: RRID:AB_626632 WB/IF: Anti-NeuN (rabbit monoclonal) Abcam Cat# ab177487; RRID: AB_2532109 WB: Anti-TET1 (Rabbit polyclonal) EpigenTek Cat# A-1020 IF: Anti-TET1 - C-terminal (rabbit polyclonal) Abcam Cat# ab191698; RRID: AB_2858250 WB/IF: Anti-TBP(1TBP18) (mouse monoclonal) Santa Cruz Cat# sc-56795; RRID: AB_786004 DB: Anti-5-Methylcytosine (5-mC) (mouse monoclonal) Active Motif Cat# 61479; RRID: AB_2793653 DB/IF: Anti-5-Hydroxymethylcytosine (5- hmC) (rabbit polyclonal) Active Motif Cat# 39791; RRID: AB_2630381 IF: DIPI (4‘,6-Diamidino-2-Phenylindole, Dihydrochloride) Beyotime Cat# C1006 IF: Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen, ThermoFisher Cat# A-11029; RRID: AB_2534088 IF: Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 594 Invitrogen, ThermoFisher Cat# A-32742; RRID: AB_2762825 IF: lexa Fluor® 594 AffiniPure® Goat AntiRabbit IgG (H + L) Jackson Cat# 111-585-144; RRID:AB_2307325 IF: Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 Invitrogen, ThermoFisher Cat# A32731TR; RRID:AB_2866491 WB: HRP Conjugated AffiniPure Donkey Anti-Mouse IgG(H + L) Boster Cat# BA1062 WB: HRP Conjugated AffiniPure Donkey Anti-Rabbit IgG(H + L) Boster Cat# BA1061 Bacterial and virus strains Trans5α Chemically Competent Cell Transgen Cat# CD201-02 Chemicals, peptides, and recombinant proteins 10× TE buffer Meilunbio Cat# 1MA0011 Ampicillin sodium salt VMR Cat# 10339-100G AMPure XP beads Beckman Cat# A63882 Bovine serum albumin Solarbio Cat# 1A8020 DMEM medium Gibco, ThermoFisher Cat# 11965118 DynabeadsTM MyOneTM Streptavidin C1 Life Technologies, Thermo Scientific Cat# 65002 EDTA BBI, Sangon Biotech Cat# 1A600142-0500 (Continued on next page) 14 Cell Reports 45, 117584, July 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER EGTA VWR Cat# 10732 Fetal bovine serum Gibco, ThermoFisher Cat# 16000044 HEPES Solarbio Cat# H8090 Hygromycin B Gibco Cat# 110687010 LipofectamineTM 3000 Invitrogen, ThermoFisher Cat# L3000015 Methylene blue Sigma-Aldrich Cat# 77720-25G NaCl BBI, Sangon Biotech Cat# 1A610476-0005 NaOH Coolaber Cat# SL9069-500ML NON-Fat Powdered Milk BBI, Sangon Biotech Cat# A600669 NP-40 BBI, Sangon Biotech Cat# 1A600385-0100 Opti-MEMTM Gibco, ThermoFisher Cat# 31985070 Paraformaldehyde Sigma-Aldrich Cat# 158127-3KG Penicillin-Streptomycin Gibco, ThermoFisher Cat# 15140122 Phenylmethylsulfonyl fluoride (PMSF) Sigma Aldrich Cat# P7626 Protein A-agarose Thermo Scientific Cat# 20333 Proteinase K Beyotime Cat# ST532 SDS Amresco Cat# 0227-1KG Sucrose Sangon Biotech Cat# A502792-0005 T4 β-glucosyltransferase New England Biolabs Cat# M0357L TaKaRa EpiTaqTM HS Takara Cat# R110B Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat# 78444 Tissue-Tek® O.C.T. .. Compound Takara Cat# 4583 Tris BBI, Sangon Biotech Cat# 1A100193-0005 Triton X-100 Amresco Cat# 0694-1L TRIzolTM reagent Invitrogen, ThermoFisher Cat# 15596018CN Trypsin-EDTA (0.25%) Gibco, ThermoFisher Cat# 25200072 Tween 20 Solarbio Cat# 1T8220 UDP-6-N3-Glu Jena Bioscience Cat# CLK-076 TET1 inhibitor Medchem Express Cat# HY-122258 NSC-370284 Critical commercial assays PrimeScriptTM RT reagent Kit Takara Cat# RR037B QuantiNova Probe PCR Kit Qiagen Cat# 208052 PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) Takara Cat# RR047B Luciferase Assay System reagent Promega Cat# E1500 MethylCodeTM Bisulfite Conversion Kit ThermoFisher Cat# MECOV50 Qubit High Sensitivity Kit ThermoFisher Cat# Q33230 NEBNext® UltraTM II DNA Library Prep Kit New England Biolabs Cat# E7645S MinElute PCR Purification Kit Qiagen Cat# 28006 QIAquick Nucleotide Removal Kit Qiagen Cat# 28306 Deposited data 5mC sequencing This paper Database: BioProject Accession #: PRJNA1454197 5hmC sequencing This paper Database: BioProject Accession #: PRJNA1455152 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 N2a ATCC Cat# CCL-131 (Continued on next page) Cell Reports 45, 117584, July 28, 2026 15

    other:

    Article Title: <scp> <i>RRAGD</i> </scp> p.( <scp>Ser76Leu</scp> ) Variant Causes Dysregulated Expression of Muscle Development and Cytoskeleton Genes in Cardiomyocytes
    Article Snippet: 1.8 × 106 T- REx HeLa cells (Invitrogen, Massachusetts, USA) were seeded into a 10- cm petri dish and 6 h later, the cells were co- transfected with 1 μg pOG44 plasmid (Invitrogen, Massachusetts, USA; K650001) and 1 μg of either empty pcDNA5/FRT/TO- eGFP (mock), pcDNA5/FRT/TO- eGFP RRAGD WT, or p.(Ser76Leu) plasmid, totaling to 2 μg DNA.

    Article Title: Establishment of a Protoplast Transformation Method in the Oleaginous Yeast Rhodotorula toruloides.
    Article Snippet: YNB medium contained 1.7 g L-1 YNB without ammonium 119 sulphate (Formedium), 2.3 g L-1 K2HPO4, 11.83 g L-1 KH2PO4, 10 g L-1 ammonium sulphate, and 120 20 g L-1 glucose, adjusted to pH 5.5.



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