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rfp ef1a puro  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology rfp ef1a puro
    Rfp Ef1a Puro, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lvp429/RFP+(EF1a)-Puro+lentiviral+particles/10__3390_slash_biomedicines14040776-60-2-5
    Average 98 stars, based on 3 article reviews
    rfp ef1a puro - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Photo-controlled removal of targets in vitro and in vivo
    Article Snippet: .. RFP stably expressed Balb/3T3 cells were established with transfection by RFP (EF1a)-Puro lentiviral particles (AMSBIO, Cambridge, Mass., USA). ..

    Transfection:

    Article Title: Photo-controlled removal of targets in vitro and in vivo
    Article Snippet: .. RFP stably expressed Balb/3T3 cells were established with transfection by RFP (EF1a)-Puro lentiviral particles (AMSBIO, Cambridge, Mass., USA). ..

    Expressing:

    Article Title: Impact of Silver-Coated Dressing on Adipose-Derived Stem Cells and Fibroblasts in 2D and 3D Cultures
    Article Snippet: The ADSC transfection was performed using GFP (EF1a)-Puro (LVP426, Amsbio, Abingdon, UK) in order to express the green fluorescent protein with emission at 525 nm. .. For NHDF, RFP (EF1a)-Puro (LVP429, Amsbio) red fluorescent protein expression with emission at 620 nm was used. ..



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    <t>Ki67/nuclei</t> <t>fluorescent</t> area in 2D culture systems. ( A ) ADSC monocultures—the ratio significantly increased following P3 treatment compared to untreated <t>ADSCs</t> and decreased after P2 treatment; ( B ) NHDF monocultures—no significant ratio shifts; ( C ) A80N20 coculture—no significant ratio shifts; ( D ) A20N80 cocultures—the ratios decreased after P2 treatment compared to untreated conditions (image acquisition was performed 5 days after treatment; for each condition, a minimum of 2 images were analyzed; * p ˂ 0.0002); the results represent the overall immuno-fluorescence of Ki67 normalized to the total Hoechst area, i.e., a surrogate for total cell number; all data are displayed as means with standard errors (SEM; UT—untreated).
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    Image Search Results


    Ki67/nuclei fluorescent area in 2D culture systems. ( A ) ADSC monocultures—the ratio significantly increased following P3 treatment compared to untreated ADSCs and decreased after P2 treatment; ( B ) NHDF monocultures—no significant ratio shifts; ( C ) A80N20 coculture—no significant ratio shifts; ( D ) A20N80 cocultures—the ratios decreased after P2 treatment compared to untreated conditions (image acquisition was performed 5 days after treatment; for each condition, a minimum of 2 images were analyzed; * p ˂ 0.0002); the results represent the overall immuno-fluorescence of Ki67 normalized to the total Hoechst area, i.e., a surrogate for total cell number; all data are displayed as means with standard errors (SEM; UT—untreated).

    Journal: Biomedicines

    Article Title: Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone–Iodine and Chlorhexidine in Mono and Coculture Models

    doi: 10.3390/biomedicines11071855

    Figure Lengend Snippet: Ki67/nuclei fluorescent area in 2D culture systems. ( A ) ADSC monocultures—the ratio significantly increased following P3 treatment compared to untreated ADSCs and decreased after P2 treatment; ( B ) NHDF monocultures—no significant ratio shifts; ( C ) A80N20 coculture—no significant ratio shifts; ( D ) A20N80 cocultures—the ratios decreased after P2 treatment compared to untreated conditions (image acquisition was performed 5 days after treatment; for each condition, a minimum of 2 images were analyzed; * p ˂ 0.0002); the results represent the overall immuno-fluorescence of Ki67 normalized to the total Hoechst area, i.e., a surrogate for total cell number; all data are displayed as means with standard errors (SEM; UT—untreated).

    Article Snippet: The lentivirus contained an elongation factor 1α as promoter, a puromycin resistance gene, and either a green fluorescent protein gene (LVP426, Amsbio, Abingdon, UK) for ADSCs or a red fluorescent protein gene (LVP429, Amsbio, Abingdon, UK) for NHDFs.

    Techniques: Fluorescence