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SuperArray Bioscience Corporation rt2 first strand kit
Rt2 First Strand Kit, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt2 first strand kit - by Bioz Stars, 2026-10
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Reverse Transcription Polymerase Chain Reaction:

Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway
Article Snippet: .. The RNA samples were kept on ice for another 5 min and then converted into cDNA with the RT PCR Array First Strand Kit (SuperArray) according to the manufacturer’s protocol. .. cDNA (20 ng) was combined with RT SYBR Green/Fluorescein PCR master mix (SuperArray), and equal amounts of this mixture (25 μL) were added to each well of the RT Profiler PCR plate containing the predispensed gene-specific primer sets.

RNA Extraction:

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, Md.). ..

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, MD). ..

Synthesized:

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, Md.). ..

Article Title: Inhibition of acid sphingomyelinase by imipramine abolishes the synergy between metabolic syndrome and periodontitis on alveolar bone loss.
Article Snippet: .. First- strand cDNA was synthesized from RNA using RT2 First Strand Kit (SuperArray Bioscience Corp.). .. Mouse toll- like receptor (TLR) pathway- focused PCR Arrays (SuperArray Bioscience Corp.) were performed using 2× SuperArray RT2 qPCR master mix and the first- strand cDNA by following the instructions from the manufacturer.

Article Title: GPR40 deficiency worsens metabolic syndrome-associated periodontitis in mice.
Article Snippet: © 2023 John Wiley & Sons Ltd.. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.. 1Division of Endocrinology, Diabetes and Metabolic Diseases, Department of Medicine, College of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA 2Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, USA 3Department of Head & Neck/Plastic & Reconstructive Surgery, Roswell Park Comprehensive Cancer Center, Buffalo, New York, USA 4National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, USA 5Integrative Physiology and Neuroscience, Washington State University, Pullman, Washington, USA 6Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina, USA

Article Title: Vaccine and methods for detecting and preventing filariasis
Article Snippet: After 72 hours, cells were centrifuged (1000 rpm for 5 minutes) and total RNA was extracted from the cell pellets using TRIZOL (phenol, guanidinium and thiocyanate) reagent (Invitrogen) as per description of the manufacturer. .. Followed by RNA extraction, first-strand cDNA was synthesized by RT2 First Strand Kit (SuperArray Bioscience Corporation, Frederick, MD). ..

Polymerase Chain Reaction:

Article Title: Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway.
Article Snippet: .. The RNA samples were kept on ice for another 5 min and then converted into cDNA with the RT2 PCR Array First Strand Kit (SuperArray) according to the manufacturer’s protocol. cDNA (20 ng) was combined with RT2 SYBR Green/ Fluorescein PCR master mix (SuperArray), and equal amounts of this mixture (25 μL) were added to each well of the RT2 Profiler PCR plate containing the predispensed gene-specific primer sets. ..

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.

Concentration Assay:

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.

Reverse Transcription:

Article Title: Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Article Snippet: .. Thereafter, the RNA concentration of the pools was measured, and 1 μg of total RNA from each pool was reverse transcribed using the RT 2 PCR Array First Strand Kit (C-02, SuperArray Bioscience, Frederick, MD, USA). .. The generated cDNA was diluted with an appropriate volume of instrument-specific 2x SuperArray RT2 Real-TimeTM SYBR Green PCR Master Mix (PA-012, SuperArray Bioscience, Frederick, MD, USA) and ultra-pure water, and 25 μL of this reaction mix was added to each well of the PCR array.



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Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n <t>RT-qPCR</t> of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM
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Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Loss of type I IFN responsiveness impairs natural killer cell antitumor activity in breast cancer

doi: 10.1007/s00262-021-02857-z

Figure Lengend Snippet: Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

Article Snippet: When required, cells were transfected with poly I:C (10 μg/ml) overnight, prior to RNA extraction. cDNA was generated using the iScript Reverse Transcriptase Supermix cDNA for RT-qPCR kit (BioRad).

Techniques: Cell Function Assay, Injection, Staining, Expressing, Quantitative RT-PCR, IV Injection, Fluorescence, Imaging