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v v ipg buffer  (Cytiva Europe)


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    Cytiva Europe v v ipg buffer
    V V Ipg Buffer, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 3282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipg+buffer/IPG+buffer/pmc03350990-73-18-24
    Average 95 stars, based on 3282 article reviews
    v v ipg buffer - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: AMH regulates ovary size by counteracting the positive influence of clustered ovarian follicle growth.
    Article Snippet: Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots (625 ng) of recombinant human AMH (produced in HEK293 cells by PX Therapeutics under contract to University of Otago, �20% proAMH, 80% AMHN, C) were combined with 130 μl of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/l urea, 2 mol/l thiourea, 2% w/v CHAPS, 50 mM DTT, 5 mmol/l TCEP (triscarboxyethylphosphine), to which 25 μl of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 ml of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3–10, 7 cm, linear gradient IEF strips (GE Healthcare). .. The rehydrated IEF strips were then subjected to electrophoresis in an IEF flatbed electrophoresis system (IPGphor, Pharmacia Biotech) in a ceramic-welled plate with the IEF strips submerged under mineral oil, and using a voltage programme from 200 to 8000 V over 10 h, with a V/h target of 30 000 V/h.

    Produced:

    Article Title: AMH regulates ovary size by counteracting the positive influence of clustered ovarian follicle growth.
    Article Snippet: Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots (625 ng) of recombinant human AMH (produced in HEK293 cells by PX Therapeutics under contract to University of Otago, �20% proAMH, 80% AMHN, C) were combined with 130 μl of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/l urea, 2 mol/l thiourea, 2% w/v CHAPS, 50 mM DTT, 5 mmol/l TCEP (triscarboxyethylphosphine), to which 25 μl of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 ml of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3–10, 7 cm, linear gradient IEF strips (GE Healthcare). .. The rehydrated IEF strips were then subjected to electrophoresis in an IEF flatbed electrophoresis system (IPGphor, Pharmacia Biotech) in a ceramic-welled plate with the IEF strips submerged under mineral oil, and using a voltage programme from 200 to 8000 V over 10 h, with a V/h target of 30 000 V/h.

    Electrofocusing:

    Article Title: AMH regulates ovary size by counteracting the positive influence of clustered ovarian follicle growth.
    Article Snippet: Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots (625 ng) of recombinant human AMH (produced in HEK293 cells by PX Therapeutics under contract to University of Otago, �20% proAMH, 80% AMHN, C) were combined with 130 μl of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/l urea, 2 mol/l thiourea, 2% w/v CHAPS, 50 mM DTT, 5 mmol/l TCEP (triscarboxyethylphosphine), to which 25 μl of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 ml of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3–10, 7 cm, linear gradient IEF strips (GE Healthcare). .. The rehydrated IEF strips were then subjected to electrophoresis in an IEF flatbed electrophoresis system (IPGphor, Pharmacia Biotech) in a ceramic-welled plate with the IEF strips submerged under mineral oil, and using a voltage programme from 200 to 8000 V over 10 h, with a V/h target of 30 000 V/h.



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